{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T15:44:36Z","timestamp":1784907876376,"version":"3.55.0"},"reference-count":57,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:00:00Z","timestamp":1679616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Health and ISCIII","award":["FIS-PI17-01819"],"award-info":[{"award-number":["FIS-PI17-01819"]}]},{"name":"Spanish Ministry of Health and ISCIII","award":["FIS-PI20-01690"],"award-info":[{"award-number":["FIS-PI20-01690"]}]},{"name":"Spanish Ministry of Health and ISCIII","award":["EuroNanoMed III AC17-0027"],"award-info":[{"award-number":["EuroNanoMed III AC17-0027"]}]},{"name":"Spanish Ministry of Health and ISCIII","award":["RD16\/0012\/0011"],"award-info":[{"award-number":["RD16\/0012\/0011"]}]},{"name":"FOREUM, Foundation for Research in Rheumatology","award":["FIS-PI17-01819"],"award-info":[{"award-number":["FIS-PI17-01819"]}]},{"name":"FOREUM, Foundation for Research in Rheumatology","award":["FIS-PI20-01690"],"award-info":[{"award-number":["FIS-PI20-01690"]}]},{"name":"FOREUM, Foundation for Research in Rheumatology","award":["EuroNanoMed III AC17-0027"],"award-info":[{"award-number":["EuroNanoMed III AC17-0027"]}]},{"name":"FOREUM, Foundation for Research in Rheumatology","award":["RD16\/0012\/0011"],"award-info":[{"award-number":["RD16\/0012\/0011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by the generation of anti-DNA autoantibodies due to exposure of immune cells to excessive amounts of extracellular DNA. Lack of P-selectin in mice induces the development of a lupus-like syndrome and patients with cutaneous lupus have reduced P-selectin expression in skin vessels. Using flow cytometry we analyzed in healthy donors and patients the expression of P-selectin Glycoprotein Ligand-1 (PSGL-1) in circulating neutrophils and the implication of PSGL-1\/P-selectin interaction in neutrophil extracellular traps (NETs) generation. We found a statistical significance that neutrophils from active SLE patients have a reduced expression of PSGL-1 and low levels of PSGL-1 in neutrophils from SLE patients associated with the presence of anti-dsDNA antibodies, clinical lung involvement, Raynaud\u2019s phenomenon, and positive lupus anticoagulant. PSGL-1 is present along the DNA in the NET. In healthy donors, neutrophil interaction with immobilized P-selectin triggers Syk activation, increases the NETs percentage and reduces the amount of DNA extruded in the NETs. In active SLE patients, neutrophil interaction with P-selectin does not activate Syk or reduce the amount of DNA extruded in the NETs, that might contribute to increase the extracellular level of DNA and hence, to disease pathogenesis.<\/jats:p>","DOI":"10.3390\/ijms24076144","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T04:31:05Z","timestamp":1679632265000},"page":"6144","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Low P-Selectin Glycoprotein Ligand-1 Expression in Neutrophils Associates with Disease Activity and Deregulated NET Formation in Systemic Lupus Erythematosus"],"prefix":"10.3390","volume":"24","author":[{"given":"Antonio","family":"Mu\u00f1oz-Callejas","sequence":"first","affiliation":[{"name":"Immunology Department, Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica (FIB), Instituto de Investigaci\u00f3n Sanitaria-Princesa (IIS-Princesa), Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Gonz\u00e1lez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Immunology Department, Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica (FIB), Instituto de Investigaci\u00f3n Sanitaria-Princesa (IIS-Princesa), Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Silv\u00e1n","sequence":"additional","affiliation":[{"name":"Immunology Department, Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica (FIB), Instituto de Investigaci\u00f3n Sanitaria-Princesa (IIS-Princesa), Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8667-7247","authenticated-orcid":false,"given":"Esther","family":"San Antonio","sequence":"additional","affiliation":[{"name":"Immunology Department, Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica (FIB), Instituto de Investigaci\u00f3n Sanitaria-Princesa (IIS-Princesa), Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Gonz\u00e1lez-Tajuelo","sequence":"additional","affiliation":[{"name":"Immunology Department, Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica (FIB), Instituto de Investigaci\u00f3n Sanitaria-Princesa (IIS-Princesa), Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6376-058X","authenticated-orcid":false,"given":"Alejandra","family":"Ramos-Manzano","sequence":"additional","affiliation":[{"name":"Immunology Department, Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica (FIB), Instituto de Investigaci\u00f3n Sanitaria-Princesa (IIS-Princesa), Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u00e9s","family":"S\u00e1nchez-Abad","sequence":"additional","affiliation":[{"name":"Immunology Department, Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica (FIB), Instituto de Investigaci\u00f3n Sanitaria-Princesa (IIS-Princesa), Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9614-5199","authenticated-orcid":false,"given":"Isidoro","family":"Gonz\u00e1lez-Alvaro","sequence":"additional","affiliation":[{"name":"Rheumatology Department, Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica (FIB), Instituto de Investigaci\u00f3n Sanitaria-Princesa (IIS-Princesa), Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0026-1043","authenticated-orcid":false,"given":"Javier","family":"Garc\u00eda-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Pulmonology Department, Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica (FIB), Instituto de Investigaci\u00f3n Sanitaria-Princesa (IIS-Princesa), Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva G.","family":"Tomero","sequence":"additional","affiliation":[{"name":"Rheumatology Department, Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica (FIB), Instituto de Investigaci\u00f3n Sanitaria-Princesa (IIS-Princesa), Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9096-3780","authenticated-orcid":false,"given":"Rosario Garc\u00eda","family":"de 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Princesa, 28006 Madrid, Spain"},{"name":"Rheumatology Department, Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica (FIB), Instituto de Investigaci\u00f3n Sanitaria-Princesa (IIS-Princesa), Hospital de la Princesa, 28006 Madrid, Spain"},{"name":"Catedra UAM-Roche, EPID-Future, Department of Medicine, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9326-6112","authenticated-orcid":false,"given":"Ana","family":"Urzainqui","sequence":"additional","affiliation":[{"name":"Immunology Department, Fundaci\u00f3n de Investigaci\u00f3n Biom\u00e9dica (FIB), Instituto de Investigaci\u00f3n Sanitaria-Princesa (IIS-Princesa), Hospital de la Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1007\/s00441-017-2753-2","article-title":"Partners in crime: Neutrophils and monocytes\/macrophages in inflammation and disease","volume":"371","author":"Nicholls","year":"2018","journal-title":"Cell Tissue Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1126\/science.1092385","article-title":"Neutrophil extracellular traps kill bacteria","volume":"303","author":"Brinkmann","year":"2004","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2344","DOI":"10.1016\/S0140-6736(19)30546-X","article-title":"Novel paradigms in systemic lupus 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