{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:08:09Z","timestamp":1791590889975,"version":"4.3.4"},"reference-count":25,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,3,26]],"date-time":"2023-03-26T00:00:00Z","timestamp":1679788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japan Society for the Promotion of Science","award":["19K18859"],"award-info":[{"award-number":["19K18859"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Zinn\u2019s zonule is a fragile and thin tissue, and little is known about its pathogenesis. The aim of this study was to develop an experimental setup for a comprehensive analysis of Zinn\u2019s zonule. Rats were divided into two groups: a control group (n = 4) and an alkali injury group (n = 4). Seven days after injury, the eyes were enucleated, the anterior eye was dissected and embedded in gelatin, and macroscopic observations were made. The gelatin specimens were then embedded in paraffin and observed in detail by low-vacuum scanning electron microscopy, immunofluorescence, and quantitative reverse transcription polymerase chain reaction (RT-qPCR). The results show qualitative changes in Zinn\u2019s zonules in both macroscopic and microscopic observations. In addition, macrophage infiltration and increased matrix metalloproteinase 2 (MMP2) expression were observed in the injured group, consistent with the RT-qPCR results. The experimental system in this study allowed us to capture the morphological and molecular biological changes of Zinn\u2019s zonule and to gain insight into its pathogenesis. In conclusion, this study presents a new experimental setup for the comprehensive analysis of the rat Zinn\u2019s zonule. The results suggest that this system can be used in the future to study and analyze a variety of paraffin-embedded tissues and specimens.<\/jats:p>","DOI":"10.3390\/ijms24076254","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T04:05:40Z","timestamp":1679889940000},"page":"6254","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Novel Multi-Observation System to Study the Effects of Anterior Ocular Inflammation in Zinn\u2019s Zonule Using One Specimen"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7538-4059","authenticated-orcid":false,"given":"Akira","family":"Takahashi","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Nippon Medical School, Tokyo 113-8602, Japan"},{"name":"Department of Analytic Human Pathology, Nippon Medical School, Tokyo 113-8602, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1006-4430","authenticated-orcid":false,"given":"Takeshi","family":"Arima","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Nippon Medical School, Tokyo 113-8602, Japan"},{"name":"Department of Analytic Human Pathology, Nippon Medical School, Tokyo 113-8602, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3091-8686","authenticated-orcid":false,"given":"Etsuko","family":"Toda","sequence":"additional","affiliation":[{"name":"Department of Analytic Human Pathology, Nippon Medical School, Tokyo 113-8602, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichiro","family":"Kobayakawa","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Nippon Medical School, Musashikosugi Hospital, Kanagawa 211-8533, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4364-9251","authenticated-orcid":false,"given":"Akira","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Analytic Human Pathology, Nippon Medical School, Tokyo 113-8602, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Nippon Medical School, Tokyo 113-8602, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"805706","DOI":"10.1155\/2015\/805706","article-title":"Epidemiology, etiology, and prevention of late IOL-capsular bag complex dislocation: Review of the literature","volume":"2015","author":"Ascaso","year":"2015","journal-title":"J. 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