{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T12:02:26Z","timestamp":1785931346058,"version":"3.56.0"},"reference-count":47,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T00:00:00Z","timestamp":1679961600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Science Foundation","award":["N23-26-10018"],"award-info":[{"award-number":["N23-26-10018"]}]},{"name":"Krasnoyarsk Regional Science Foundation","award":["N23-26-10018"],"award-info":[{"award-number":["N23-26-10018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The current paper considers the effects of a water-soluble polymer (polyethylene glycol (PEG)) on the bioluminescent reaction of the photoprotein obelin from the marine coelenterate Obelia longissima and the product of this bioluminescent reaction: a coelenteramide-containing fluorescent protein (CCFP). We varied PEG concentrations (0\u20131.44 mg\/mL) and molecular weights (1000, 8000, and 35,000 a.u.). The presence of PEG significantly increased the bioluminescent intensity of obelin but decreased the photoluminescence intensity of CCFP; the effects did not depend on the PEG concentration or the molecular weight. The photoluminescence spectra of CCFP did not change, while the bioluminescence spectra changed in the course of the bioluminescent reaction. The changes can be explained by different rigidity of the media in the polymer solutions affecting the stability of the photoprotein complex and the efficiency of the proton transfer in the bioluminescent reaction. The results predict and explain the change in the luminescence intensity and color of the marine coelenterates in the presence of water-soluble polymers. The CCFP appeared to be a proper tool for the toxicity monitoring of water-soluble polymers (e.g., PEGs).<\/jats:p>","DOI":"10.3390\/ijms24076345","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T02:58:21Z","timestamp":1679972301000},"page":"6345","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Water-Soluble Polymer Polyethylene Glycol: Effect on the Bioluminescent Reaction of the Marine Coelenterate Obelia and Coelenteramide-Containing Fluorescent Protein"],"prefix":"10.3390","volume":"24","author":[{"given":"Tatiana S.","family":"Siniakova","sequence":"first","affiliation":[{"name":"Biophysics Department, Siberian Federal University, 660041 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander V.","family":"Raikov","sequence":"additional","affiliation":[{"name":"Biophysics Department, Siberian Federal University, 660041 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5315-8002","authenticated-orcid":false,"given":"Nadezhda S.","family":"Kudryasheva","sequence":"additional","affiliation":[{"name":"Biophysics Department, Siberian Federal University, 660041 Krasnoyarsk, Russia"},{"name":"Institute of Biophysics SB RAS, FRC KSC SB RAS, 660036 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"64","DOI":"10.17516\/1997-1389-0186","article-title":"Thermoinactivation of Coupled Enzyme System of Luminous Bacteria NADH:FMN-Oxidoreductase-Luciferase in Gelatin","volume":"4","author":"Bezrukikh","year":"2011","journal-title":"J. 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