{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T01:27:56Z","timestamp":1777166876463,"version":"3.51.4"},"reference-count":87,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T00:00:00Z","timestamp":1679961600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)"},{"name":"Coordination for the Improvement of Higher Education Personnel"},{"name":"Brazil (CAPES)\u2014Finance Code 001"},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)"},{"name":"Carlos Chagas Filho Research Foundation of the State of Rio de Janeiro (FAPERJ)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Several cytogenetic studies have already been performed in Triatominae, such that different karyotypes could be characterized (ranging from 2n = 21 to 25 chromosomes), being the changes in the number of chromosomes related mainly to fusion and fission events. These changes have been associated with reproductive isolation and speciation events in other insect groups. Thus, we evaluated whether different karyotypes could act in the reproductive isolation of triatomines and we analyzed how the events of karyotypic evolution occurred along the diversification of these vectors. For this, experimental crosses were carried out between triatomine species with different karyotypes. Furthermore, based on a phylogeny with 88 triatomine taxa (developed with different molecular markers), a reconstruction of ancestral karyotypes and of anagenetic and cladogenetic events related to karyotypic alterations was performed through the ChromoSSE chromosomal evolution model. All crosses performed did not result in hybrids (prezygotic isolation in both directions). Our modeling results suggest that during Triatominae diversification, at least nine cladogenetic events may be associated with karyotype change. Thus, we emphasize that these alterations in the number of chromosomes can act as a prezygotic barrier in Triatominae (karyotypic isolation), being important evolutionary events during the diversification of the species of Chagas disease vectors.<\/jats:p>","DOI":"10.3390\/ijms24076350","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T04:37:15Z","timestamp":1679978235000},"page":"6350","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Karyotype Evolution in Triatominae (Hemiptera, Reduviidae): The Role of Chromosomal Rearrangements in the Diversification of Chagas Disease Vectors"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1861-6703","authenticated-orcid":false,"given":"Yago Visinho dos","family":"Reis","sequence":"first","affiliation":[{"name":"Instituto de Bioci\u00eancias de Botucatu, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Rua Dr. Ant\u00f4nio Celso Wagner Zanin, 250, Distrito de Rubi\u00e3o Junior, Botucatu 18618-689, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2588-1911","authenticated-orcid":false,"given":"Jader","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Entomologia em Sa\u00fade P\u00fablica, Faculdade de Sa\u00fade P\u00fablica, Universidade de S\u00e3o Paulo (USP), Av. Dr. Arnaldo 715, S\u00e3o Paulo 01246-904, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Fernandez","family":"Madeira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular, Instituto de Bioci\u00eancias, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Letras e Ci\u00eancias Exatas, Rua Crist\u00f3v\u00e3o Colombo 2265, S\u00e3o Jos\u00e9 do Rio Preto 15054-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda","family":"Ravazi","sequence":"additional","affiliation":[{"name":"Instituto de Bioci\u00eancias de Botucatu, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Rua Dr. Ant\u00f4nio Celso Wagner Zanin, 250, Distrito de Rubi\u00e3o Junior, Botucatu 18618-689, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Beatriz Bortolozo de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular, Instituto de Bioci\u00eancias, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Letras e Ci\u00eancias Exatas, Rua Crist\u00f3v\u00e3o Colombo 2265, S\u00e3o Jos\u00e9 do Rio Preto 15054-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isadora da Silva","family":"Bittinelli","sequence":"additional","affiliation":[{"name":"Instituto de Bioci\u00eancias de Botucatu, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Rua Dr. Ant\u00f4nio Celso Wagner Zanin, 250, Distrito de Rubi\u00e3o Junior, Botucatu 18618-689, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiza Maria Grzyb","family":"Delgado","sequence":"additional","affiliation":[{"name":"Instituto de Bioci\u00eancias de Botucatu, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Rua Dr. Ant\u00f4nio Celso Wagner Zanin, 250, Distrito de Rubi\u00e3o Junior, Botucatu 18618-689, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Terc\u00edlia Vilela","family":"de Azeredo-Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular, Instituto de Bioci\u00eancias, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Letras e Ci\u00eancias Exatas, Rua Crist\u00f3v\u00e3o Colombo 2265, S\u00e3o Jos\u00e9 do Rio Preto 15054-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Aristeu da","family":"Rosa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia, Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Rodovia Araraquara-Ja\u00fa km 1, Araraquara 14801-902, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4027-9205","authenticated-orcid":false,"given":"Cleber","family":"Galv\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Nacional e Internacional de Refer\u00eancia em Taxonomia de Triatom\u00edneos, Instituto Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, Pavilh\u00e3o Rocha Lima, sala 505, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaio Cesar Chaboli","family":"Alevi","sequence":"additional","affiliation":[{"name":"Instituto de Bioci\u00eancias de Botucatu, Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d (UNESP), Rua Dr. Ant\u00f4nio Celso Wagner Zanin, 250, Distrito de Rubi\u00e3o Junior, Botucatu 18618-689, SP, Brazil"},{"name":"Laborat\u00f3rio de Entomologia em Sa\u00fade P\u00fablica, Faculdade de Sa\u00fade P\u00fablica, Universidade de S\u00e3o Paulo (USP), Av. Dr. Arnaldo 715, S\u00e3o Paulo 01246-904, SP, Brazil"},{"name":"Laborat\u00f3rio Nacional e Internacional de Refer\u00eancia em Taxonomia de Triatom\u00edneos, Instituto Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, Pavilh\u00e3o Rocha Lima, sala 505, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1016\/S0140-6736(10)60061-X","article-title":"Chagas disease","volume":"375","author":"Rassi","year":"2010","journal-title":"Lancet"},{"key":"ref_2","unstructured":"World Health Organization (2022, October 06). Chagas Disease (American trypanosomiasis). Available online: http:\/\/www.who.int\/news-room\/fact-sheets\/detail\/chagas-disease-(american-trypanosomiasis)."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Galv\u00e3o, C. (2014). 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