{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T03:30:01Z","timestamp":1788838201664,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T00:00:00Z","timestamp":1679961600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Development Program Project of Jilin Province","award":["20200201389JC"],"award-info":[{"award-number":["20200201389JC"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["IJMS"],"abstract":"<jats:p>Periodontitis is a long-term inflammatory illness and a leading contributor to tooth loss in humans. Due to the influence of the anatomic parameters of teeth, such as root bifurcation lesions and the depth of the periodontal pocket, basic periodontal treatment on its own often does not completely obliterate flora microorganisms. As a consequence, topical medication has become a significant supplement in the treatment of chronic periodontitis. Berberine (BBR) has various pharmacological effects, such as hypoglycemic, antitumor, antiarrhythmic, anti-inflammatory, etc. The target of our project is to develop a safe and non-toxic carrier that can effectively release berberine, which can significantly reduce periodontal tissue inflammation, and to investigate whether berberine thermosensitive hydrogel can exert anti-inflammatory and osteogenic effects by modulating phosphatifylinositol-3-kinase\/Protein Kinase B (PI3K\/AKT) signaling pathway. Consequently, firstly berberine temperature-sensitive hydrogel was prepared, and its characterizations showed that the mixed solution gelated within 3 min under 37 \u00b0C with a hole diameter of 10\u2013130 \u00b5m, and the accumulation of berberine release amounted to 89.99% at 21 days. CCK-8 and live-dead cell staining results indicated that this hydrogel was not biotoxic, and it is also presumed that the optimum concentration of berberine is 5 \u00b5M, which was selected for subsequent experiments. Real-time polymerase chain reaction (qRT-PCR) and Western blotting (WB)results demonstrated that inflammatory factors, as well as protein levels, were significantly reduced in the berberine-loaded hydrogel group, and LY294002 (PI3K inhibitor) could enhance this effect (p &lt; 0.05). In the berberine-loaded hydrogel group, osteogenesis-related factor levels and protein profiles were visibly increased, along with an increase in alkaline phosphatase expression, which was inhibited by LY294002 (p &lt; 0.05). Therefore, berberine thermosensitive hydrogel may be an effective treatment for periodontitis, and it may exert anti-inflammatory and osteogenic effects through the PI3K\/AKT signaling pathway.<\/jats:p>","DOI":"10.3390\/ijms24076364","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T07:05:25Z","timestamp":1679987125000},"page":"6364","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Role of Berberine Thermosensitive Hydrogel in Periodontitis via PI3K\/AKT Pathway In Vitro"],"prefix":"10.3390","volume":"24","author":[{"given":"Chang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Periodontology, Hospital of Stomatology, Jilin University, 1500 Tsinghua Road, Chaoyang District, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Hospital of Stomatology, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Hospital of Stomatology, Jilin University, 1500 Tsinghua Road, Chaoyang District, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyuan","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Hospital of Stomatology, Jilin University, 1500 Tsinghua Road, Chaoyang District, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinying","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Endodontics, Hospital of Stomatology, Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Hospital of Stomatology, Jilin University, 1500 Tsinghua Road, Chaoyang District, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Hospital of Stomatology, Jilin University, 1500 Tsinghua Road, Chaoyang District, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Hospital of Stomatology, Jilin University, 1500 Tsinghua Road, Chaoyang District, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1057\/jcb.2010.37","article-title":"The economic potential of plant-made pharmaceuticals in the manufacture of biologic pharmaceuticals","volume":"17","author":"Kaufman","year":"2011","journal-title":"J. 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