{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T18:12:00Z","timestamp":1760551920916,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T00:00:00Z","timestamp":1680134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Mexican government for the Children\u2019s Hospital of Mexico Federico G\u00f3mez","award":["HIM\/2013\/015.SSa.1061"],"award-info":[{"award-number":["HIM\/2013\/015.SSa.1061"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Asthma is a heterogeneous entity encompassing distinct endotypes and varying phenotypes, characterized by common clinical manifestations, such as shortness of breath, wheezing, and variable airflow obstruction. Two major asthma endotypes based on molecular patterns are described: type 2 endotype (allergic-asthma) and T2 low endotype (obesity-related asthma). Long noncoding RNAs (lncRNAs) are transcripts of more than 200 nucleotides in length, currently involved in many diverse biological functions, such as chromatin remodeling, gene transcription, protein transport, and microRNA processing. Despite the efforts to accurately classify and discriminate all the asthma endotypes and phenotypes, if long noncoding RNAs could play a role as biomarkers in allergic asthmatic and adolescent obesity-related asthma, adolescents remain unknown. To compare expression levels of lncRNAs: HOTAIRM1, OIP5-AS1, MZF1-AS1, and GAS5 from whole blood of Healthy Adolescents (HA), Obese adolescents (O), allergic asthmatic adolescents (AA) and Obesity-related asthma adolescents (OA). We measured and compared expression levels from the whole blood of the groups mentioned above through RT-q-PCR. We found differentially expressed levels of these lncRNAs between the groups of interest. In addition, we found a discriminative value of previously mentioned lncRNAs between studied groups. Finally, we generated an interaction network through bioinformatics. Expression levels of OIP5-AS1, MZF1-AS1, HOTAIRM1, and GAS5 in whole blood from the healthy adolescent population, obese adolescents, allergic asthma adolescents, and obesity-related asthma adolescents are differently expressed. Moreover, these lncRNAs could act as molecular biomarkers that help to discriminate between all studied groups, probably through molecular mechanisms with several genes and miRNAs implicated.<\/jats:p>","DOI":"10.3390\/ijms24076481","type":"journal-article","created":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T03:17:35Z","timestamp":1680146255000},"page":"6481","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Whole Blood Expression Levels of Long Noncoding RNAs: HOTAIRM1, GAS5, MZF1-AS1, and OIP5-AS1 as Biomarkers in Adolescents with Obesity-Related Asthma"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7178-1786","authenticated-orcid":false,"given":"Jos\u00e9 J.","family":"Leija-Mart\u00ednez","sequence":"first","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Obesidad y Asma, Hospital Infantil de M\u00e9xico Federico G\u00f3mez, Ciudad de M\u00e9xico 06720, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7365-9023","authenticated-orcid":false,"given":"Carlos A.","family":"Guzm\u00e1n-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Departamento de Inmunolog\u00eda, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7541-8773","authenticated-orcid":false,"given":"Javier","family":"Gonz\u00e1lez-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Celular, Facultad de Enfermer\u00eda, Universidad Aut\u00f3noma de Baja California Campus Mexicali, Mexicali 21280, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1076-6731","authenticated-orcid":false,"given":"Abraham","family":"Giacoman-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Obesidad y Asma, Hospital Infantil de M\u00e9xico Federico G\u00f3mez, Ciudad de M\u00e9xico 06720, Mexico"},{"name":"Laboratorio de Farmacolog\u00eda, Departamento de Ciencias de la Salud, DCBS, Universidad Aut\u00f3noma Metropolitana-Iztapalapa (UAM-I), Ciudad de M\u00e9xico 09340, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Blanca E.","family":"Del-R\u00edo-Navarro","sequence":"additional","affiliation":[{"name":"Departamento de Inmunolog\u00eda Cl\u00ednica de Alergia Pedi\u00e1trica, Hospital Infantil de M\u00e9xico Federico G\u00f3mez, Ciudad de M\u00e9xico 06720, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo","family":"Romero-Nava","sequence":"additional","affiliation":[{"name":"Laboratorio de Se\u00f1alizaci\u00f3n Intracelular, Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Ciudad de M\u00e9xico 07738, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santiago","family":"Villafa\u00f1a","sequence":"additional","affiliation":[{"name":"Laboratorio de Se\u00f1alizaci\u00f3n Intracelular, Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Ciudad de M\u00e9xico 07738, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Luis","family":"Flores-Saenz","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda, Departamento de Ciencias de la Salud, DCBS, Universidad Aut\u00f3noma Metropolitana-Iztapalapa (UAM-I), Ciudad de M\u00e9xico 09340, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6556-1632","authenticated-orcid":false,"given":"Fausto","family":"S\u00e1nchez-Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Departamento de Inmunolog\u00eda, Instituto Nacional de Cardiolog\u00eda Ignacio Ch\u00e1vez, Ciudad de M\u00e9xico 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6503-694X","authenticated-orcid":false,"given":"Fengyang","family":"Huang","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Obesidad y Asma, Hospital Infantil de M\u00e9xico Federico G\u00f3mez, Ciudad de M\u00e9xico 06720, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s12016-018-8712-1","article-title":"Understanding Asthma Phenotypes, Endotypes, and Mechanisms of Disease","volume":"1556","author":"Kuruvilla","year":"2019","journal-title":"Clin. 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