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In this sense, with scarce detailed data on the subfields of hippocampus in dementia neurodegeneration, there is no information about astrocytic responses in its different layers. Thus, considering this context, the present study evaluated the effects of deprivation and rehabilitation of masticatory activity, aging, and environmental enrichment on the stereological quantification of hippocampal astrocytes from layers CA1, CA3, and DG. For this purpose, we examined mature (6-month-old; 6M), and aged (18-month-old; 18M) mice, subjected to distinct masticatory regimens and environments. Three different regimens of masticatory activity were applied: continuous normal mastication with hard pellets (HD); normal mastication followed by deprived mastication with equal periods of pellets followed by soft powder (HD\/SD); or rehabilitated masticatory activity with equal periods of HD, followed by powder, followed by pellets (HD\/SD\/HD). Under each specific regimen, half of the animals were raised in standard cages (impoverished environment (IE)) and the other half in enriched cages (enriched environment (EE)), mimicking sedentary or active lifestyles. Microscopic stereological, systematic, and random sampling approaches with an optical dissector of GFAP-immunolabeled astrocytes were done, allowing for an astrocyte numerical estimate. Stratum moleculare and hilus, from the dentate gyrus (DG) and Strata Lacunosum-Moleculare, Oriens, and Radiatum, similarly to the dentate gyrus, showed no significant change in any of the investigated variables (age, diet, or environment) in these layers. However, in Stratum radiatum, it was possible to observe significant differences associated with diet regimens and age. Therefore, diet-related differences were found when the HD 18M IE group was compared to the HD\/SD\/HD 18-month-old group in the same environment (IE) (p = 0.007). In the present study, we present modulatory factors (masticatory function, environmental enrichment, and aging) for the differentiated quantitative laminar response in the hippocampal regions, suggesting other studies to read the plasticity and responsiveness of astrocytes, including the molecular background.<\/jats:p>","DOI":"10.3390\/ijms24076529","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T02:30:20Z","timestamp":1680229820000},"page":"6529","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Masticatory Activity Interference in Quantitative Estimation of CA1, CA3 and Dentate Gyrus Hippocampal Astrocytes of Aged Murine Models and under Environmental Stimulation"],"prefix":"10.3390","volume":"24","author":[{"given":"Mar\u00edlia da","family":"Cunha Feio Leal","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00f5es em Neurodegenera\u00e7\u00e3o e Infec\u00e7\u00e3o, Instituto de Ci\u00eancias Biol\u00f3gicas, Hospital Universit\u00e1rio Jo\u00e3o de Barros Barreto, Universidade Federal do Par\u00e1, Bel\u00e9m 66073-005, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2755-2801","authenticated-orcid":false,"given":"Fabio Leite do","family":"Amaral Junior","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00f5es em Neurodegenera\u00e7\u00e3o e Infec\u00e7\u00e3o, Instituto de Ci\u00eancias Biol\u00f3gicas, Hospital Universit\u00e1rio Jo\u00e3o de Barros Barreto, Universidade Federal do Par\u00e1, Bel\u00e9m 66073-005, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernardo Freire da","family":"Silva Arruda","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00f5es em Neurodegenera\u00e7\u00e3o e Infec\u00e7\u00e3o, Instituto de Ci\u00eancias Biol\u00f3gicas, Hospital Universit\u00e1rio Jo\u00e3o de Barros Barreto, Universidade Federal do Par\u00e1, Bel\u00e9m 66073-005, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Ayumi Azevedo","family":"Kurosawa","sequence":"additional","affiliation":[{"name":"Curso de Medicina, Centro Universit\u00e1rio do Estado do Par\u00e1, Bel\u00e9m 66613-903, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Almeida","family":"Vieira","sequence":"additional","affiliation":[{"name":"Curso de Medicina, Centro Universit\u00e1rio do Estado do Par\u00e1, Bel\u00e9m 66613-903, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00falia Corr\u00eaa Campos","family":"Maia","sequence":"additional","affiliation":[{"name":"Curso de Medicina, Centro Universit\u00e1rio do Estado do Par\u00e1, Bel\u00e9m 66613-903, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana Virg\u00ednia Bezerra","family":"Scalfoni","sequence":"additional","affiliation":[{"name":"Curso de Medicina, Centro Universit\u00e1rio do Estado do Par\u00e1, Bel\u00e9m 66613-903, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Morais da","family":"Silveira Junior","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00f5es em Neurodegenera\u00e7\u00e3o e Infec\u00e7\u00e3o, Instituto de Ci\u00eancias Biol\u00f3gicas, Hospital Universit\u00e1rio Jo\u00e3o de Barros Barreto, Universidade Federal do Par\u00e1, Bel\u00e9m 66073-005, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus Oliveira","family":"Feij\u00f3","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00f5es em Neurodegenera\u00e7\u00e3o e Infec\u00e7\u00e3o, Instituto de Ci\u00eancias Biol\u00f3gicas, Hospital Universit\u00e1rio Jo\u00e3o de Barros Barreto, Universidade Federal do Par\u00e1, Bel\u00e9m 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