{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:02:47Z","timestamp":1792789367807,"version":"4.5.0"},"reference-count":48,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Angelman Syndrome Alliance (ASA)","award":["2021"],"award-info":[{"award-number":["2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Intranasal drug delivery is convenient and provides a high bioavailability but requires the use of mucoadhesive nanocarriers. Chitosan is a well-established polymer for mucoadhesive applications but can suffer from poor cytocompatibility and stability upon administration. In this work, we present a method to obtain stable and cytocompatible crosslinked chitosan nanoparticles. We used 2,6-pyridinedicarboxylic acid as a biocompatible crosslinker and compared the obtained particles with those prepared by ionotropic gelation using sodium tripolyphosphate. Nanoparticles were tested to evaluate the size and the surface charge, as well as their stability in storage conditions (4 \u00b0C), at the nasal cavity temperature (32 \u00b0C), and at the body temperature (37 \u00b0C). The crosslinked chitosan nanoparticles showed a size around 150 nm and a surface charge of 10.3 mV \u00b1 0.9 mV, both compatible with the intranasal drug administration. Size and surface charge parameters did not significantly vary over time, indicating the good stability of these nanoparticles. We finally tested their cytocompatibility in vitro using SHSY5Y human neuroblastoma and RPMI 2650 human nasal epithelial cells, with positive results. In conclusion, the proposed synthetic system shows an interesting potential as a drug carrier for intranasal delivery.<\/jats:p>","DOI":"10.3390\/ijms24076590","type":"journal-article","created":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T03:08:31Z","timestamp":1680491311000},"page":"6590","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Crosslinked Chitosan Nanoparticles with Muco-Adhesive Potential for Intranasal Delivery Applications"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5860-3141","authenticated-orcid":false,"given":"Mariacristina","family":"Gagliardi","sequence":"first","affiliation":[{"name":"National Enterprise for nanoScience and nanoTechnology (NEST), Nanoscience Institute\u2014National Research Council (CNR) and Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0131-8275","authenticated-orcid":false,"given":"Sara","family":"Chiarugi","sequence":"additional","affiliation":[{"name":"National Enterprise for nanoScience and nanoTechnology (NEST), Nanoscience Institute\u2014National Research Council (CNR) and Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"De Cesari","sequence":"additional","affiliation":[{"name":"National Enterprise for nanoScience and nanoTechnology (NEST), Nanoscience Institute\u2014National Research Council (CNR) and Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5349-814X","authenticated-orcid":false,"given":"Giulia","family":"Di Gregorio","sequence":"additional","affiliation":[{"name":"National Enterprise for nanoScience and nanoTechnology (NEST), Nanoscience Institute\u2014National Research Council (CNR) and Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6997-4176","authenticated-orcid":false,"given":"Alessandra","family":"Diodati","sequence":"additional","affiliation":[{"name":"National Enterprise for nanoScience and nanoTechnology (NEST), Nanoscience Institute\u2014National Research Council (CNR) and Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6739-2860","authenticated-orcid":false,"given":"Laura","family":"Baroncelli","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, National Research Council (CNR), Via Giuseppe Moruzzi 1, 56124 Pisa, Italy"},{"name":"Department of Developmental Neuroscience, Scientific Institute Stella Maris Foundation, Viale del Tirreno 331, Calambrone, 56128 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9688-2356","authenticated-orcid":false,"given":"Marco","family":"Cecchini","sequence":"additional","affiliation":[{"name":"National Enterprise for nanoScience and nanoTechnology (NEST), Nanoscience Institute\u2014National Research Council (CNR) and Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7323-2174","authenticated-orcid":false,"given":"Ilaria","family":"Tonazzini","sequence":"additional","affiliation":[{"name":"National Enterprise for nanoScience and nanoTechnology (NEST), Nanoscience Institute\u2014National Research Council (CNR) and Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Montegiove, N., Calzoni, E., Emiliani, C., and Cesaretti, A. 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