{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T19:32:30Z","timestamp":1785353550013,"version":"3.55.0"},"reference-count":68,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T00:00:00Z","timestamp":1680480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Government Contract","award":["FWRM-2021-0006"],"award-info":[{"award-number":["FWRM-2021-0006"]}]},{"name":"Government Contract","award":["121032300337-5"],"award-info":[{"award-number":["121032300337-5"]}]},{"name":"Government Contract","award":["122022800132-1"],"award-info":[{"award-number":["122022800132-1"]}]},{"name":"State Assignment of the Ministry of Health of the Russian Federation","award":["FWRM-2021-0006"],"award-info":[{"award-number":["FWRM-2021-0006"]}]},{"name":"State Assignment of the Ministry of Health of the Russian Federation","award":["121032300337-5"],"award-info":[{"award-number":["121032300337-5"]}]},{"name":"State Assignment of the Ministry of Health of the Russian Federation","award":["122022800132-1"],"award-info":[{"award-number":["122022800132-1"]}]},{"name":"State Assignment of the Russian Federation","award":["FWRM-2021-0006"],"award-info":[{"award-number":["FWRM-2021-0006"]}]},{"name":"State Assignment of the Russian Federation","award":["121032300337-5"],"award-info":[{"award-number":["121032300337-5"]}]},{"name":"State Assignment of the Russian Federation","award":["122022800132-1"],"award-info":[{"award-number":["122022800132-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This paper focuses on the surface modification of the Ti-6Al-4V alloy substrate via a-C:H:SiOx coating deposition. Research results concern the a-C:H:SiOx coating structure, investigated using transmission electron microscopy and in vitro endothelization to study the coating. Based on the analysis of the atomic radial distribution function, a model is proposed for the atomic short-range order structure of the a-C:H:SiOx coating, and chemical bonds (C\u2013O, C\u2013C, Si\u2013C, Si\u2013O, and Si\u2013Si) are identified. It is shown that the a-C:H:SiOx coating does not possess prolonged cytotoxicity in relation to EA.hy926 endothelial cells. In vitro investigations showed that the adhesion, cell number, and nitric oxide production by EA.hy926 endothelial cells on the a-C:H:SiOx-coated Ti-6Al-4V substrate are significantly lower than those on the uncoated surface. The findings suggest that the a-C:H:SiOx coating can reduce the risk of endothelial cell hyperproliferation on implants and medical devices, including mechanical prosthetic heart valves, endovascular stents, and mechanical circulatory support devices.<\/jats:p>","DOI":"10.3390\/ijms24076675","type":"journal-article","created":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T02:30:15Z","timestamp":1680575415000},"page":"6675","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Endothelial Cell Behavior and Nitric Oxide Production on a-C:H:SiOx-Coated Ti-6Al-4V Substrate"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3465-8452","authenticated-orcid":false,"given":"Igor A.","family":"Khlusov","sequence":"first","affiliation":[{"name":"Laboratory of Cellular and Microfluidic Technologies, Siberian State Medical University, 2, Moskovskii Tract, 634050 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6013-0200","authenticated-orcid":false,"given":"Alexander S.","family":"Grenadyorov","sequence":"additional","affiliation":[{"name":"The Institute of High Current Electronics SB RAS, 2\/3, Akademichesky Ave., 634055 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7775-9769","authenticated-orcid":false,"given":"Andrey A.","family":"Solovyev","sequence":"additional","affiliation":[{"name":"The Institute of High Current Electronics SB RAS, 2\/3, Akademichesky Ave., 634055 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vyacheslav A.","family":"Semenov","sequence":"additional","affiliation":[{"name":"The Institute of High Current Electronics SB RAS, 2\/3, Akademichesky Ave., 634055 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maksim O.","family":"Zhulkov","sequence":"additional","affiliation":[{"name":"E.N. Meshalkin National Medical Research Center of Ministry of Health of Russian Federation, 15, Rechkunovskaya Str., 630055 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry A.","family":"Sirota","sequence":"additional","affiliation":[{"name":"E.N. Meshalkin National Medical Research Center of Ministry of Health of Russian Federation, 15, Rechkunovskaya Str., 630055 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0540-3357","authenticated-orcid":false,"given":"Aleksander M.","family":"Chernyavskiy","sequence":"additional","affiliation":[{"name":"E.N. Meshalkin National Medical Research Center of Ministry of Health of Russian Federation, 15, Rechkunovskaya Str., 630055 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga V.","family":"Poveshchenko","sequence":"additional","affiliation":[{"name":"E.N. Meshalkin National Medical Research Center of Ministry of Health of Russian Federation, 15, Rechkunovskaya Str., 630055 Novosibirsk, Russia"},{"name":"Research Institute of Clinical and Experimental Lymphology, Branch of Institute of Cytology and Genetics SB RAS, 2, Timakov Str., 630060 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4752-988X","authenticated-orcid":false,"given":"Maria A.","family":"Surovtseva","sequence":"additional","affiliation":[{"name":"E.N. Meshalkin National Medical Research Center of Ministry of Health of Russian Federation, 15, Rechkunovskaya Str., 630055 Novosibirsk, Russia"},{"name":"Research Institute of Clinical and Experimental Lymphology, Branch of Institute of Cytology and Genetics SB RAS, 2, Timakov Str., 630060 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina I.","family":"Kim","sequence":"additional","affiliation":[{"name":"E.N. Meshalkin National Medical Research Center of Ministry of Health of Russian Federation, 15, Rechkunovskaya Str., 630055 Novosibirsk, Russia"},{"name":"Research Institute of Clinical and Experimental Lymphology, Branch of Institute of Cytology and Genetics SB RAS, 2, Timakov Str., 630060 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalya A.","family":"Bondarenko","sequence":"additional","affiliation":[{"name":"E.N. 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