{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:54:50Z","timestamp":1785804890315,"version":"3.56.0"},"reference-count":48,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T00:00:00Z","timestamp":1680739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Joint Funds of the National Natural Science Foundation of China","award":["U1804114"],"award-info":[{"award-number":["U1804114"]}]},{"name":"Joint Funds of the National Natural Science Foundation of China","award":["GZS2020007"],"award-info":[{"award-number":["GZS2020007"]}]},{"name":"Joint Funds of the National Natural Science Foundation of China","award":["HARS-22-09-G1"],"award-info":[{"award-number":["HARS-22-09-G1"]}]},{"name":"Henan Province Outstanding Foreign Scholar Program","award":["U1804114"],"award-info":[{"award-number":["U1804114"]}]},{"name":"Henan Province Outstanding Foreign Scholar Program","award":["GZS2020007"],"award-info":[{"award-number":["GZS2020007"]}]},{"name":"Henan Province Outstanding Foreign Scholar Program","award":["HARS-22-09-G1"],"award-info":[{"award-number":["HARS-22-09-G1"]}]},{"name":"Special Fund for Henan Agricultural Research System","award":["U1804114"],"award-info":[{"award-number":["U1804114"]}]},{"name":"Special Fund for Henan Agricultural Research System","award":["GZS2020007"],"award-info":[{"award-number":["GZS2020007"]}]},{"name":"Special Fund for Henan Agricultural Research System","award":["HARS-22-09-G1"],"award-info":[{"award-number":["HARS-22-09-G1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Controlling the tree size of fruit species such as peach can reduce the amount of labor and input needed for orchard management. The phytohormone gibberellin (GA) positively regulates tree size by inducing degradation of the GA signaling repressor DELLA. The N-terminal DELLA domain in this protein is critical for its GA-dependent interaction with the GA receptor GID1 and the resulting degradation of the DELLA protein, which allows for growth-promoting GA signaling. In this study, a DELLA family member, PpeDGYLA, contains a DELLA domain but has amino acid changes in three conserved motifs (DELLA into DGYLA, LEQLE into LERLE, and TVHYNP into AVLYNP). In the absence or presence of GA3, the PpeDGYLA protein did not interact with PpeGID1c and was stable in 35S-PpeDGYLA peach transgenic callus. The overexpression of PpeDGYLA in both polar and Arabidopsis showed an extremely dwarfed phenotype, and these transgenic plants were insensitive to GA3 treatment. PpeDGYLA could interact with PpeARF6-1 and -2, supposed growth-promoting factors. It is suggested that the changes in the DELLA domain of PpeDGYLA may, to some extent, account for the severe dwarf phenotype of poplar and Arabidopsis transgenic plants. In addition, our study showed that the DELLA family contained three clades (DELLA-like, DELLA, and DGLLA). PpeDGYLA clustered into the DGLLA clade and was expressed in all of the analyzed tissues. These results lay the foundation for the further study of the repression of tree size by PpeDGYLA.<\/jats:p>","DOI":"10.3390\/ijms24076789","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T02:29:58Z","timestamp":1680748198000},"page":"6789","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Peach DELLA Protein PpeDGYLA Is Not Degraded in the Presence of Active GA and Causes Dwarfism When Overexpressed in Poplar and Arabidopsis"],"prefix":"10.3390","volume":"24","author":[{"given":"Yun","family":"Chen","sequence":"first","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengmeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Shao","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianbo","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Tan","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0503-5467","authenticated-orcid":false,"given":"Jidong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Li","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Langlang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7468-8491","authenticated-orcid":false,"given":"Jiancan","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/nph.13772","article-title":"A brachytic dwarfism trait (dw) in peach trees is caused by a nonsense mutation within the gibberellic acid receptor PpeGID1c","volume":"210","author":"Hollender","year":"2015","journal-title":"New Phytol."},{"key":"ref_2","first-page":"46","article-title":"Understanding how rootstocks dwarf fruit trees","volume":"34","author":"Atkinson","year":"2001","journal-title":"Compact. Fruit Tree"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"151","DOI":"10.17660\/ActaHortic.2001.557.19","article-title":"Lethal sensitivity of some new cherry rootstocks to pollen-borne viruses","volume":"557","author":"Lang","year":"2001","journal-title":"Acta Hortic."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1007\/s11248-010-9370-0","article-title":"Effects of transgenic rootstocks on growth and development of non-transgenic scion cultivars in apple","volume":"19","author":"Smolka","year":"2010","journal-title":"Transgenic Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1111\/pbi.13094","article-title":"A single nucleotide mutation in GID1c disrupts its interaction with DELLA1 and causes a GA-insensitive dwarf phenotype in peach","volume":"17","author":"Cheng","year":"2019","journal-title":"Plant Biotechnol. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1104\/pp.112.200956","article-title":"Gibberellin Signaling: A Theme and Variations on DELLA Repression","volume":"160","author":"Hauvermale","year":"2012","journal-title":"Plant Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3058","DOI":"10.1105\/tpc.107.051524","article-title":"The GID1-Mediated Gibberellin Perception Mechanism Is Conserved in the Lycophyte Selaginella moellendorffii but Not in the Bryophyte Physcomitrella patens","volume":"19","author":"Hirano","year":"2007","journal-title":"Plant Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1105\/tpc.13.5.999","article-title":"slender Rice, a Constitutive Gibberellin Response Mutant, Is Caused by a Null Mutation of the SLR1 Gene, an Ortholog of the Height-Regulating Gene GAI\/RGA\/RHT\/D8","volume":"13","author":"Ikeda","year":"2001","journal-title":"Plant Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s00497-018-0333-6","article-title":"Control of grain size in rice","volume":"31","author":"Li","year":"2018","journal-title":"Plant Reprod."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1146\/annurev.arplant.59.032607.092804","article-title":"Gibberellin Metabolism and its Regulation","volume":"59","author":"Yamaguchi","year":"2008","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"9043","DOI":"10.1073\/pnas.132266399","article-title":"Semidwarf (sd-1), \u201cgreen revolution\u201d rice, contains a defective gibberellin 20-oxidase gene","volume":"99","author":"Spielmeyer","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2028","DOI":"10.1104\/pp.114.247486","article-title":"The Maize DWARF1 Encodes a Gibberellin 3-Oxidase and Is Dual Localized to the Nucleus and Cytosol","volume":"166","author":"Chen","year":"2014","journal-title":"Plant Physiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1111\/j.1365-313X.2007.03356.x","article-title":"The gibberellin biosynthetic genes AtGA20ox1 and AtGA20ox2 act, partially redundantly, to promote growth and development throughout the Arabidopsis life cycle","volume":"53","author":"Rieu","year":"2008","journal-title":"Plant J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1104\/pp.103.020354","article-title":"Activation Tagging of a Dominant Gibberellin Catabolism Gene (GA 2-oxidase) from Poplar That Regulates Tree Stature","volume":"132","author":"Busov","year":"2003","journal-title":"Plant Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/nature04028","article-title":"GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin","volume":"437","author":"Ashikari","year":"2005","journal-title":"Nature"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1105\/tpc.010319","article-title":"The Gibberellin Signaling Pathway Is Regulated by the Appearance and Disappearance of SLENDER RICE1 in Nuclei","volume":"14","author":"Itoh","year":"2002","journal-title":"Plant Cell"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1111\/j.1365-313X.2003.01990.x","article-title":"GID2, an F-box subunit of the SCF E3 complex, specifically interacts with phosphorylated SLR1 protein and regulates the gibberellin-dependent degradation of SLR1 in rice","volume":"37","author":"Gomi","year":"2004","journal-title":"Plant J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1104\/pp.110.161554","article-title":"Gibberellin-GID1-DELLA: A Pivotal Regulatory Module for Plant Growth and Development","volume":"154","author":"Sun","year":"2010","journal-title":"Plant Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/nature06520","article-title":"A molecular framework for light and gibberellin control of cell elongation","volume":"451","author":"Pontin","year":"2008","journal-title":"Nature"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1038\/nature06448","article-title":"Coordinated regulation of Arabidopsis thaliana development by light and gibberellins","volume":"451","author":"Feng","year":"2008","journal-title":"Nature"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e3031","DOI":"10.7554\/eLife.03031","article-title":"Cell elongation is regulated through a central circuit of interacting transcription factors in the Arabidopsis hypocotyl","volume":"3","author":"Oh","year":"2014","journal-title":"Elife"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Vera-Sirera, F., Gomez, M.D., and Perez-Amador, M.A. (2016). DELLA Proteins, a Group of GRAS Transcription Regulators that Mediate Gibberellin Signaling\u2014ScienceDirect. Plant Transcr. Factors, 313\u2013328.","DOI":"10.1016\/B978-0-12-800854-6.00020-8"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2140","DOI":"10.1105\/tpc.106.043729","article-title":"Molecular Interactions of a Soluble Gibberellin Receptor, GID1, with a Rice DELLA Protein, SLR1, and Gibberellin","volume":"19","author":"Nakajima","year":"2007","journal-title":"Plant Cell"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1093\/molbev\/msz009","article-title":"Origin of Gibberellin-Dependent Transcriptional Regulation by Molecular Exploitation of a Transactivation Domain in DELLA Proteins","volume":"36","author":"Dumas","year":"2019","journal-title":"Mol. Biol. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/j.1365-313X.2012.05000.x","article-title":"The suppressive function of the rice DELLA protein SLR1 is dependent on its transcriptional activation activity","volume":"71","author":"Hirano","year":"2012","journal-title":"Plant J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1038\/416847a","article-title":"Association of dwarfism and floral induction with a grape \u2018green revolution\u2019 mutation","volume":"416","author":"Boss","year":"2002","journal-title":"Nature"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"111003","DOI":"10.1016\/j.scienta.2022.111003","article-title":"Genome-wide analysis of the GRAS transcription factor gene family in peach (Prunus persica) and ectopic expression of PpeDELLA1 and PpeDELLA2 in Arabidopsis result in dwarf phenotypes","volume":"298","author":"Jiang","year":"2022","journal-title":"Sci. Hortic."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1038\/ncb2546","article-title":"Brassinosteroid, gibberellin and phytochrome impinge on a common transcription module in Arabidopsis","volume":"14","author":"Bai","year":"2012","journal-title":"Nat. Cell Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"111409","DOI":"10.1016\/j.plantsci.2022.111409","article-title":"PpPIF8, a DELLA2-interacting protein, regulates peach shoot elongation possibly through auxin signaling","volume":"323","author":"Chen","year":"2022","journal-title":"Plant Sci."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Zhang, M., Ma, Y., Zheng, X., Bin Tan, B., Ye, X., Wang, W., Zhang, L., Li, J., Li, Z., and Cheng, J. (2022). The distribution of bioactive gibberellins along peach annual shoots is closely associated with PpGA20ox and PpGA2ox expression profiles. BMC Genom., 23.","DOI":"10.1186\/s12864-022-08943-5"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1007\/s00425-004-1203-z","article-title":"The role of GRAS proteins in plant signal transduction and development","volume":"218","author":"Bolle","year":"2004","journal-title":"Planta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1038\/nature07519","article-title":"Gibberellin-induced DELLA recognition by the gibberellin receptor GID1","volume":"456","author":"Murase","year":"2008","journal-title":"Nature"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2680","DOI":"10.1105\/tpc.110.075549","article-title":"Characterization of the Molecular Mechanism Underlying Gibberellin Perception Complex Formation in Rice","volume":"22","author":"Hirano","year":"2010","journal-title":"Plant Cell"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"698","DOI":"10.4161\/psb.4.8.9176","article-title":"GRAS-domain transcription factors that regulate plant development","volume":"4","author":"Hirsch","year":"2009","journal-title":"Plant Signal. Behav."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1111\/j.1365-313X.2005.02562.x","article-title":"Overexpression of a GRAS protein lacking the DELLA domain confers altered gibberellin responses in rice","volume":"44","author":"Itoh","year":"2005","journal-title":"Plant J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"14162","DOI":"10.1073\/pnas.251534098","article-title":"The DELLA motif is essential for gibberellin-induced degradation of RGA","volume":"98","author":"Dill","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.jplph.2011.09.012","article-title":"Functional characterization of the apple MhGAI1 gene through ectopic expression and grafting experiments in tomatoes","volume":"169","author":"Wang","year":"2012","journal-title":"J. Plant Physiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2369","DOI":"10.1007\/s11738-012-1041-8","article-title":"Ectopic expression of the apple mhgai2 gene brings about GA-insensitive phenotypes in tomatoes","volume":"34","author":"Wang","year":"2012","journal-title":"Acta Physiol. Plant."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1111\/ede.12365","article-title":"Evolution of DELLA function and signaling in land plants","volume":"23","author":"Phokas","year":"2021","journal-title":"Evol. Dev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1093\/jxb\/eru493","article-title":"Knockdown of a JmjC domain-containing gene JMJ524 confers altered gibberellin responses by transcriptional regulation of GRAS protein lacking the DELLA domain genes in tomato","volume":"66","author":"Li","year":"2015","journal-title":"J. Exp. Bot."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1016\/j.tplants.2017.07.010","article-title":"Exploiting DELLA Signaling in Cereals","volume":"22","author":"Ruelens","year":"2017","journal-title":"Trends Plant Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1046\/j.1365-313x.1998.00343.x","article-title":"Floral dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana","volume":"16","author":"Clough","year":"1998","journal-title":"Plant J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1093\/treephys\/tpq093","article-title":"Enhanced resistance to fungal pathogens in transgenic Populus tomentosa Carr. by overexpression of an nsLTP-like antimicrobial protein gene from motherwort (Leonurus japonicus)","volume":"30","author":"Jia","year":"2010","journal-title":"Tree Physiol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Sun, Y., Ren, S., Ye, S., Tian, Q., and Luo, K. (2020). Identification and Functional Characterization of PtoMYB055 Involved in the Regulation of the Lignin Biosynthesis Pathway in Populus tomentosa. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21144857"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Tian, Q., Wang, X., Li, C., Lu, W., Yang, L., Jiang, Y., and Luo, K. (2013). Functional Characterization of the Poplar R2R3-MYB Transcription Factor PtoMYB216 Involved in the Regulation of Lignin Biosynthesis during Wood Formation. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0076369"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2836","DOI":"10.1038\/s41598-020-59626-8","article-title":"Development of a fast and efficient root transgenic system for functional genomics and genetic engineering in peach","volume":"10","author":"Xu","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2522","DOI":"10.1002\/pmic.200300789","article-title":"A critical evaluation of sample extraction techniques for enhanced proteomic analysis of recalcitrant plant tissues","volume":"4","author":"Saravanan","year":"2004","journal-title":"Proteomics"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1111\/ppa.12786","article-title":"Identification of wheat blue dwarf phytoplasma effectors targeting plant proliferation and defence responses","volume":"67","author":"Wang","year":"2017","journal-title":"Plant Pathol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/7\/6789\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:10:44Z","timestamp":1760123444000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/7\/6789"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,6]]},"references-count":48,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["ijms24076789"],"URL":"https:\/\/doi.org\/10.3390\/ijms24076789","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,6]]}}}