{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T23:33:59Z","timestamp":1783035239736,"version":"3.54.6"},"reference-count":68,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T00:00:00Z","timestamp":1680739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["857418"],"award-info":[{"award-number":["857418"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["101079349"],"award-info":[{"award-number":["101079349"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>One of the most critical steps in mammalian reproduction is implantation. Embryos with an impaired capacity for embryo\u2013maternal crosstalk are thought to have a reduced potential for implantation. One agent of embryo\u2013maternal communication is extracellular vesicles (EV). EVs are lipid bilayer-bound biological nanoparticles implicated in intercellular communication between many of the known cell types. In the current study, we isolated EVs from trophoblast analogue JAr spheroids and supplemented the EVs with receptive endometrium analogue RL95-2 cells to simulate pre-implantation embryo\u2013maternal dialogue. The transcriptome of the endometrial cells was examined at 30 min, 4 h and 48 h intervals using Oxford Nanopore\u00ae technology. At the time points, 30 min, 4 h and 48 h, the endometrial cells showed a significantly altered transcriptome. It seems trophoblast EVs induce a swift and drastic effect on the endometrial transcriptome. The effect peaks at around 4 h of EV supplementation, indicating a generalized effect on cell physiology. Alterations are especially apparent in biological pathways critical to embryonic implantation, such as extracellular matrix\u2013receptor interactions and cytokine\u2013receptor interactions. These observations can be helpful in elucidating the dynamics of embryo\u2013maternal communication in the pre-implantation period.<\/jats:p>","DOI":"10.3390\/ijms24076811","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T02:29:58Z","timestamp":1680748198000},"page":"6811","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Rhythm of the First Language: Dynamics of Extracellular Vesicle-Based Embryo\u2013Maternal Communication in the Pre-Implantation Microenvironment"],"prefix":"10.3390","volume":"24","author":[{"given":"Kasun","family":"Godakumara","sequence":"first","affiliation":[{"name":"Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, 50411 Tartu, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8385-1438","authenticated-orcid":false,"given":"Paul R.","family":"Heath","sequence":"additional","affiliation":[{"name":"Sheffield Institute for Translational Neuroscience (SITRAN), University of Sheffield, 385a Glossop Rd., Broomhall, Sheffield S10 2HQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Fazeli","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, 50411 Tartu, Estonia"},{"name":"Department of Pathophysiology, Institute of Biomedicine and Translational Medicine, Faculty of Medicine, University of Tartu, 14B Ravila, 50411 Tartu, Estonia"},{"name":"Academic Unit of Reproductive and Developmental Medicine, Department of Oncology and Metabolism, The Medical School, University of Sheffield, Sheffield S10 2RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2765","DOI":"10.12688\/f1000research.8937.1","article-title":"Early embryo mortality in natural human reproduction: What the data say","volume":"5","author":"Jarvis","year":"2016","journal-title":"F1000Research"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.rbmo.2017.06.015","article-title":"The Vienna consensus: Report of an expert meeting on the development of ART laboratory performance indicators","volume":"35","author":"Apter","year":"2017","journal-title":"Reprod. 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