{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T02:28:21Z","timestamp":1789784901216,"version":"4.0.1"},"reference-count":51,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,8]],"date-time":"2023-04-08T00:00:00Z","timestamp":1680912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2019YFD1000900"],"award-info":[{"award-number":["2019YFD1000900"]}]},{"name":"National Key R&amp;D Program of China","award":["cstc2021jscx-gksbX0010"],"award-info":[{"award-number":["cstc2021jscx-gksbX0010"]}]},{"name":"National Key R&amp;D Program of China","award":["YuLinKeYan2022-14"],"award-info":[{"award-number":["YuLinKeYan2022-14"]}]},{"name":"National Key R&amp;D Program of China","award":["[2022]164"],"award-info":[{"award-number":["[2022]164"]}]},{"name":"National Key R&amp;D Program of China","award":["2020[3]01"],"award-info":[{"award-number":["2020[3]01"]}]},{"name":"Chongqing Science and Technology Commission","award":["2019YFD1000900"],"award-info":[{"award-number":["2019YFD1000900"]}]},{"name":"Chongqing Science and Technology Commission","award":["cstc2021jscx-gksbX0010"],"award-info":[{"award-number":["cstc2021jscx-gksbX0010"]}]},{"name":"Chongqing Science and Technology Commission","award":["YuLinKeYan2022-14"],"award-info":[{"award-number":["YuLinKeYan2022-14"]}]},{"name":"Chongqing Science and Technology Commission","award":["[2022]164"],"award-info":[{"award-number":["[2022]164"]}]},{"name":"Chongqing Science and Technology Commission","award":["2020[3]01"],"award-info":[{"award-number":["2020[3]01"]}]},{"name":"Chongqing Forestry Administration","award":["2019YFD1000900"],"award-info":[{"award-number":["2019YFD1000900"]}]},{"name":"Chongqing Forestry Administration","award":["cstc2021jscx-gksbX0010"],"award-info":[{"award-number":["cstc2021jscx-gksbX0010"]}]},{"name":"Chongqing Forestry Administration","award":["YuLinKeYan2022-14"],"award-info":[{"award-number":["YuLinKeYan2022-14"]}]},{"name":"Chongqing Forestry Administration","award":["[2022]164"],"award-info":[{"award-number":["[2022]164"]}]},{"name":"Chongqing Forestry Administration","award":["2020[3]01"],"award-info":[{"award-number":["2020[3]01"]}]},{"name":"Characteristic Fruit Industry and Technology System Innovation Team of Chongqing Agriculture and Rural Affairs Commission","award":["2019YFD1000900"],"award-info":[{"award-number":["2019YFD1000900"]}]},{"name":"Characteristic Fruit Industry and Technology System Innovation Team of Chongqing Agriculture and Rural Affairs Commission","award":["cstc2021jscx-gksbX0010"],"award-info":[{"award-number":["cstc2021jscx-gksbX0010"]}]},{"name":"Characteristic Fruit Industry and Technology System Innovation Team of Chongqing Agriculture and Rural Affairs Commission","award":["YuLinKeYan2022-14"],"award-info":[{"award-number":["YuLinKeYan2022-14"]}]},{"name":"Characteristic Fruit Industry and Technology System Innovation Team of Chongqing Agriculture and Rural Affairs Commission","award":["[2022]164"],"award-info":[{"award-number":["[2022]164"]}]},{"name":"Characteristic Fruit Industry and Technology System Innovation Team of Chongqing Agriculture and Rural Affairs Commission","award":["2020[3]01"],"award-info":[{"award-number":["2020[3]01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Loquat (Eriobotrya japonica Lindl.) is an evergreen fruit tree of Chinese origin, and its autumn\u2013winter flowering and fruiting growth habit means that its fruit development is susceptible to low-temperature stress. In a previous study, the triploid loquat (B431 \u00d7 GZ23) has been identified with high photosynthetic efficiency and strong resistance under low-temperature stress. Analysis of transcriptomic and lipidomic data revealed that the fatty acid desaturase gene EjFAD8 was closely associated with low temperatures. Phenotypic observations and measurements of physiological indicators in Arabidopsis showed that overexpressing-EjFAD8 transgenic plants were significantly more tolerant to low temperatures compared to the wild-type. Heterologous overexpression of EjFAD8 enhanced some lipid metabolism genes in Arabidopsis, and the unsaturation of lipids was increased, especially for SQDG (16:0\/18:1; 16:0\/18:3), thereby improving the cold tolerance of transgenic lines. The expression of ICE-CBF-COR genes were further analyzed so that the relationship between fatty acid desaturase and the ICE-CBF-COR pathway can be clarified. These results revealed the important role of EjFAD8 under low-temperature stress in triploid loquat, the increase expression of FAD8 in loquat under low temperatures lead to desaturation of fatty acids. On the one hand, overexpression of EjFAD8 in Arabidopsis increased the expression of ICE-CBF-COR genes in response to low temperatures. On the other hand, upregulation of EjFAD8 at low temperatures increased fatty acid desaturation of SQDG to maintain the stability of photosynthesis under low temperatures. This study not only indicates that the EjFAD8 gene plays an important role in loquat under low temperatures, but also provides a theoretical basis for future molecular breeding of loquat for cold resistance.<\/jats:p>","DOI":"10.3390\/ijms24086946","type":"journal-article","created":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T03:49:29Z","timestamp":1681098569000},"page":"6946","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["EjFAD8 Enhances the Low-Temperature Tolerance of Loquat by Desaturation of Sulfoquinovosyl Diacylglycerol (SQDG)"],"prefix":"10.3390","volume":"24","author":[{"given":"Xun","family":"Xu","sequence":"first","affiliation":[{"name":"Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China"},{"name":"Academy of Agricultural Sciences of Southwest 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