{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T13:20:50Z","timestamp":1774272050803,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T00:00:00Z","timestamp":1681084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Projects of Hunan Education Authorities","award":["22B0417"],"award-info":[{"award-number":["22B0417"]}]},{"name":"Projects of Hunan Education Authorities","award":["2021JJ40455"],"award-info":[{"award-number":["2021JJ40455"]}]},{"name":"Projects of Hunan Education Authorities","award":["2014-5"],"award-info":[{"award-number":["2014-5"]}]},{"name":"Projects of Hunan Education Authorities","award":["202250045337"],"award-info":[{"award-number":["202250045337"]}]},{"name":"Natural Science Foundation of Hunan Province","award":["22B0417"],"award-info":[{"award-number":["22B0417"]}]},{"name":"Natural Science Foundation of Hunan Province","award":["2021JJ40455"],"award-info":[{"award-number":["2021JJ40455"]}]},{"name":"Natural Science Foundation of Hunan Province","award":["2014-5"],"award-info":[{"award-number":["2014-5"]}]},{"name":"Natural Science Foundation of Hunan Province","award":["202250045337"],"award-info":[{"award-number":["202250045337"]}]},{"name":"Hunan Provincial Key Laboratory","award":["22B0417"],"award-info":[{"award-number":["22B0417"]}]},{"name":"Hunan Provincial Key Laboratory","award":["2021JJ40455"],"award-info":[{"award-number":["2021JJ40455"]}]},{"name":"Hunan Provincial Key Laboratory","award":["2014-5"],"award-info":[{"award-number":["2014-5"]}]},{"name":"Hunan Provincial Key Laboratory","award":["202250045337"],"award-info":[{"award-number":["202250045337"]}]},{"name":"Hengyang Science and Technology Planning Project","award":["22B0417"],"award-info":[{"award-number":["22B0417"]}]},{"name":"Hengyang Science and Technology Planning Project","award":["2021JJ40455"],"award-info":[{"award-number":["2021JJ40455"]}]},{"name":"Hengyang Science and Technology Planning Project","award":["2014-5"],"award-info":[{"award-number":["2014-5"]}]},{"name":"Hengyang Science and Technology Planning Project","award":["202250045337"],"award-info":[{"award-number":["202250045337"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chlamydia psittaci (C. psittaci), a zoonotic pathogen, poses a potential threat to public health security and the development of animal husbandry. Vaccine-based preventative measures for infectious diseases have a promising landscape. DNA vaccines, with many advantages, have become one of the dominant candidate strategies in preventing and controlling the chlamydial infection. Our previous study showed that CPSIT_p7 protein is an effective candidate for a vaccine against C. psittaci. Thus, this study evaluated the protective immunity of pcDNA3.1(+)\/CPSIT_p7 against C. psittaci infection in BALB\/c mice. We found that pcDNA3.1(+)\/CPSIT_p7 can induce strong humoral and cellular immune responses. The IFN-\u03b3 and IL-6 levels in the infected lungs of mice immunized with pcDNA3.1(+)\/CPSIT_p7 reduced substantially. In addition, the pcDNA3.1(+)\/CPSIT_p7 vaccine diminished pulmonary pathological lesions and reduced the C. psittaci load in the lungs of infected mice. It is worth noting that pcDNA3.1(+)\/CPSIT_p7 suppressed C. psittaci dissemination in BALB\/c mice. In a word, these results demonstrate that the pcDNA3.1(+)\/CPSIT_p7 DNA vaccine has good immunogenicity and immunity protection effectiveness against C. psittaci infection in BALB\/c mice, especially pulmonary infection, and provides essential practical experience and insights for the development of a DNA vaccine against chlamydial infection.<\/jats:p>","DOI":"10.3390\/ijms24087013","type":"journal-article","created":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T02:02:33Z","timestamp":1681178553000},"page":"7013","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Protective Immunity against Chlamydia psittaci Lung Infection Induced by a DNA Plasmid Vaccine Carrying CPSIT_p7 Gene Inhibits Dissemination in BALB\/c Mice"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8743-9408","authenticated-orcid":false,"given":"Chuan","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Pathogenic Biology, School of Basic Medicine, Hengyang Medical College, University of South China, Hengyang 421001, China"},{"name":"Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang 421001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingqi","family":"Jin","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology, School of Basic Medicine, Hengyang Medical College, University of South China, Hengyang 421001, China"},{"name":"Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang 421001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiewen","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology, School of Basic Medicine, Hengyang Medical College, University of South China, Hengyang 421001, China"},{"name":"Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang 421001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kang","family":"Zheng","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology, School of Basic Medicine, Hengyang Medical College, University of South China, Hengyang 421001, China"},{"name":"Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang 421001, China"},{"name":"Department of Clinical Laboratory, Hengyang Central Hospital, Hengyang 421001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aihua","family":"Lei","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology, School of Basic Medicine, Hengyang Medical College, University of South China, Hengyang 421001, China"},{"name":"Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang 421001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunxue","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology, School of Basic Medicine, Hengyang Medical College, University of South China, Hengyang 421001, China"},{"name":"Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang 421001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuzhi","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology, School of Basic Medicine, Hengyang Medical College, University of South China, Hengyang 421001, China"},{"name":"Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang 421001, China"},{"name":"Department of Pharmacology, School of Pharmaceutical Science, Hengyang Medical College, University of South China, Hengyang 421001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yimou","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology, School of Basic Medicine, Hengyang Medical College, University of South China, Hengyang 421001, China"},{"name":"Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang 421001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/femspd\/ftu007","article-title":"Chlamydia psittaci: Update on an underestimated zoonotic agent","volume":"73","author":"Knittler","year":"2015","journal-title":"Pathog. 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