{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:03:23Z","timestamp":1760148203884,"version":"build-2065373602"},"reference-count":49,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T00:00:00Z","timestamp":1681171200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The National Natural Science Foundation of China","award":["62175198","61975160","U22A2092","2022ZDLSF04-09"],"award-info":[{"award-number":["62175198","61975160","U22A2092","2022ZDLSF04-09"]}]},{"name":"Key Research and Development Program of Shaanxi Province","award":["62175198","61975160","U22A2092","2022ZDLSF04-09"],"award-info":[{"award-number":["62175198","61975160","U22A2092","2022ZDLSF04-09"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Simulated-daylight photodynamic therapy (SD-PDT) may be an efficacious strategy for treating melanoma because it can overcome the severe stinging pain, erythema, and edema experienced during conventional PDT. However, the poor daylight response of existing common photosensitizers leads to unsatisfactory anti-tumor therapeutic effects and limits the development of daylight PDT. Hence, in this study, we utilized Ag nanoparticles to adjust the daylight response of TiO2, acquire efficient photochemical activity, and then enhance the anti-tumor therapeutic effect of SD-PDT on melanoma. The synthesized Ag-doped TiO2 showed an optimal enhanced effect compared to Ag-core TiO2. Doping Ag into TiO2 produced a new shallow acceptor impurity level in the energy band structure, which expanded optical absorption in the range of 400\u2013800 nm, and finally improved the photodamage effect of TiO2 under SD irradiation. Plasmonic near-field distributions were enhanced due to the high refractive index of TiO2 at the Ag-TiO2 interface, and then the amount of light captured by TiO2 was increased to induce the enhanced SD-PDT effect of Ag-core TiO2. Hence, Ag could effectively improve the photochemical activity and SD-PDT effect of TiO2 through the change in the energy band structure. Generally, Ag-doped TiO2 is a promising photosensitizer agent for treating melanoma via SD-PDT.<\/jats:p>","DOI":"10.3390\/ijms24087061","type":"journal-article","created":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T02:57:08Z","timestamp":1681268228000},"page":"7061","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Improved Simulated-Daylight Photodynamic Therapy and Possible Mechanism of Ag-Modified TiO2 on Melanoma"],"prefix":"10.3390","volume":"24","author":[{"given":"Jing","family":"Xin","sequence":"first","affiliation":[{"name":"Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Institute of Biomedical Analytical Technology and Instrumentation, Xi\u2019an Jiaotong University, Xi\u2019an 710048, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Institute of Biomedical Analytical Technology and Instrumentation, Xi\u2019an Jiaotong University, Xi\u2019an 710048, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanping","family":"Yao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Institute of Biomedical Analytical Technology and Instrumentation, Xi\u2019an Jiaotong University, Xi\u2019an 710048, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2573-5030","authenticated-orcid":false,"given":"Sijia","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Institute of Biomedical Analytical Technology and Instrumentation, Xi\u2019an Jiaotong University, Xi\u2019an 710048, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenxi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Institute of Biomedical Analytical Technology and Instrumentation, Xi\u2019an Jiaotong University, Xi\u2019an 710048, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuiping","family":"Yao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Institute of Biomedical Analytical Technology and Instrumentation, Xi\u2019an Jiaotong University, Xi\u2019an 710048, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1615\/CritRevEukaryotGeneExpr.2020028454","article-title":"Malignant Melanoma: Skin Cancer-Diagnosis, Prevention, and Treatment","volume":"30","author":"Ahmed","year":"2020","journal-title":"Crit. Rev. Eukaryot. Gene Expr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"713","DOI":"10.2217\/fon.13.9","article-title":"Molecular networks in melanoma invasion and metastasis","volume":"9","author":"Aladowicz","year":"2013","journal-title":"Future Oncol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"391","DOI":"10.2174\/156800909788166574","article-title":"Mechanism of drug sensitivity and resistance in melanoma","volume":"9","year":"2009","journal-title":"Curr. Cancer Drug Targets"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.bbcan.2019.02.002","article-title":"Many ways to resistance: How melanoma cells evade targeted therapies","volume":"1871","author":"Kozar","year":"2019","journal-title":"Biochim. Biophys. Acta Rev. Cancer"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.1080\/15384047.2019.1640032","article-title":"Current state of melanoma diagnosis and treatment","volume":"20","author":"Davis","year":"2019","journal-title":"Cancer Biol. Ther."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.jid.2020.03.943","article-title":"Treatment of Advanced Melanoma in 2020 and Beyond","volume":"141","author":"Jenkins","year":"2021","journal-title":"J. Invest. Dermatol."},{"key":"ref_7","first-page":"561","article-title":"Photodynamic combinational therapy in cancer treatment","volume":"23","author":"Zhang","year":"2018","journal-title":"J. BUON"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.ccr.2019.05.016","article-title":"Type I photodynamic therapy by organic-inorganic hybrid materials: From strategies to applications","volume":"395","author":"Wang","year":"2019","journal-title":"Coordin. Chem. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e2006742","DOI":"10.1002\/smll.202006742","article-title":"Type I Photosensitizers Revitalizing Photodynamic Oncotherapy","volume":"17","author":"Chen","year":"2021","journal-title":"Small"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.pdpdt.2006.11.002","article-title":"The history of PDT in Norway Part one: Identification of basic mechanisms of general PDT","volume":"4","author":"Juzeniene","year":"2007","journal-title":"Photodiagn. Photodyn. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.det.2014.03.009","article-title":"Photodynamic therapy","volume":"32","author":"Rkein","year":"2014","journal-title":"Dermatol. Clin."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Rodrigues, J.A., and Correia, J.H. (2022). Enhanced Photodynamic Therapy: A Review of Combined Energy Sources. Cells, 11.","DOI":"10.3390\/cells11243995"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Algorri, J.F., Ochoa, M., Roldan-Varona, P., Rodriguez-Cobo, L., and Lopez-Higuera, J.M. (2021). Light Technology for Efficient and Effective Photodynamic Therapy: A Critical Review. Cancers, 13.","DOI":"10.3390\/cancers13143484"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1111\/php.13219","article-title":"Light Sources and Dosimetry Techniques for Photodynamic Therapy","volume":"96","author":"Kim","year":"2020","journal-title":"Photochem. Photobiol."},{"key":"ref_15","first-page":"353","article-title":"Photodynamic therapy induces less pain in patients treated with methyl aminolevulinate compared to aminolevulinic acid","volume":"5","author":"Kasche","year":"2006","journal-title":"J. Drugs Dermatol."},{"key":"ref_16","first-page":"61","article-title":"Painless versus conventional photodynamic therapy for treatment of actinic keratosis: Comparison of cell death and immune response in a murine model","volume":"10860","author":"Anand","year":"2019","journal-title":"Proc. SPIE Int. Soc. Opt. Eng."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1042\/BST20200822","article-title":"Daylight-PDT: Everything under the sun","volume":"50","author":"Beiki","year":"2022","journal-title":"Biochem. Soc. Trans."},{"key":"ref_18","first-page":"e15879","article-title":"Fully home-based methyl aminolevulinate daylight photodynamic therapy for actinic keratosis of the face or scalp: A real life open study","volume":"35","author":"Gilaberte","year":"2022","journal-title":"Dermatol. Ther."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"adv00355","DOI":"10.2340\/00015555-3717","article-title":"Repetitive Daylight Photodynamic Therapy versus Cryosurgery for Prevention of Actinic Keratoses in Photodamaged Facial Skin: A Prospective, Randomized Controlled Multicentre Two-armed Study","volume":"101","author":"Karrer","year":"2021","journal-title":"Acta Derm. Venereol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"O\u2019Mahoney, P., Khazova, M., Eadie, E., and Ibbotson, S. (2019). Measuring Daylight: A Review of Dosimetry in Daylight Photodynamic Therapy. Pharmaceuticals, 12.","DOI":"10.3390\/ph12040143"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1111\/phpp.12792","article-title":"Measurements of illuminance in simulated daylight photodynamic therapy","volume":"38","author":"Sjoholm","year":"2022","journal-title":"Photodermatol. Photoimmunol. Photomed."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1590\/abd1806-4841.20175175","article-title":"Daylight photodynamic therapy\u2014Experience and safety in treatment of actinic keratoses of the face and scalp in low latitude and high brightness region","volume":"92","author":"Galvao","year":"2017","journal-title":"An. Bras. Dermatol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.2217\/nnm-2019-0094","article-title":"The effectiveness and safety of X-PDT for cutaneous squamous cell carcinoma and melanoma","volume":"14","author":"Shi","year":"2019","journal-title":"Nanomedicine"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ziental, D., Czarczynska-Goslinska, B., Mlynarczyk, D.T., Glowacka-Sobotta, A., Stanisz, B., Goslinski, T., and Sobotta, L. (2020). Titanium Dioxide Nanoparticles: Prospects and Applications in Medicine. Nanomaterials, 10.","DOI":"10.20944\/preprints202002.0271.v1"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3447","DOI":"10.2147\/IJN.S249441","article-title":"Biomedical Applications of TiO2 Nanostructures: Recent Advances","volume":"15","author":"Jafari","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s40820-019-0362-1","article-title":"Insights into Theranostic Properties of Titanium Dioxide for Nanomedicine","volume":"12","author":"Kafshgari","year":"2020","journal-title":"Nano-Micro Lett."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Paul, S., Rahman, M.A., Bin Sharif, S., Kim, J.H., Siddiqui, S.E.T., and Hossain, M.A. (2022). TiO2 as an Anode of High-Performance Lithium-Ion Batteries: A Comprehensive Review towards Practical Application. Nanomaterials, 12.","DOI":"10.3390\/nano12122034"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1093\/nsr\/nwaa021","article-title":"Recent advances in the synthesis of hierarchically mesoporous TiO(2) materials for energy and environmental applications","volume":"7","author":"Zhang","year":"2020","journal-title":"Natl. Sci. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1574-6968.1985.tb00864.x","article-title":"Photoelectrochemical Sterilization of Microbial-Cells by Semiconductor Powders","volume":"29","author":"Matsunaga","year":"1985","journal-title":"Fems. Microbiol. Lett."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Wang, P., Zhang, L.W., Zhang, Z.X., Wang, S.J., and Yao, C.P. (2022). Influence of Parameters on Photodynamic Therapy of Au@TiO2-HMME Core-Shell Nanostructures. Nanomaterials, 12.","DOI":"10.3390\/nano12081358"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Yang, C.C., Tsai, M.H., Li, K.Y., Hou, C.H., and Lin, F.H. (2019). Carbon-Doped TiO(2) Activated by X-Ray Irradiation for the Generation of Reactive Oxygen Species to Enhance Photodynamic Therapy in Tumor Treatment. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20092072"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"128001","DOI":"10.1117\/1.JBO.21.12.128001","article-title":"Sensitized TiO2 nanocomposites through HMME linkage for photodynamic effects","volume":"21","author":"He","year":"2016","journal-title":"J. Biomed. Opt."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Liao, C., Li, Y., and Tjong, S.C. (2020). Visible-Light Active Titanium Dioxide Nanomaterials with Bactericidal Properties. Nanomaterials, 10.","DOI":"10.3390\/nano10010124"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"58738","DOI":"10.18632\/oncotarget.17639","article-title":"Visible-light-sensitive titanium dioxide nanoplatform for tumor-responsive Fe2+ liberating and artemisinin delivery","volume":"8","author":"Zhang","year":"2017","journal-title":"Oncotarget"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4622","DOI":"10.1021\/am405510a","article-title":"Structure of nitrogen and zirconium co-doped titania with enhanced visible-light photocatalytic activity","volume":"6","author":"Zhang","year":"2014","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"41253","DOI":"10.1038\/srep41253","article-title":"UV and visible light photocatalytic activity of Au\/TiO(2) nanoforests with Anatase\/Rutile phase junctions and controlled Au locations","volume":"7","author":"Yu","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"11293","DOI":"10.1039\/D0CS00912A","article-title":"Silver nanomaterials: Synthesis and (electro\/photo) catalytic applications","volume":"50","author":"Sharma","year":"2021","journal-title":"Chem. Soc. Rev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"479","DOI":"10.2147\/CCID.S401206","article-title":"Global Trends and Research Progress of Photodynamic Therapy in Skin Cancer: A Bibliometric Analysis and Literature Review","volume":"16","author":"Sun","year":"2023","journal-title":"Clin. Cosmet. Investig. Dermatol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Lerche, C.M., Heerfordt, I.M., Heydenreich, J., and Wulf, H.C. (2016). Alternatives to Outdoor Daylight Illumination for Photodynamic Therapy--Use of Greenhouses and Artificial Light Sources. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17030309"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1111\/bjd.15146","article-title":"Use of illuminance as a guide to effective light delivery during daylight photodynamic therapy in the U.K.","volume":"176","author":"Khazova","year":"2017","journal-title":"Br. J. Dermatol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1007\/s13555-022-00783-1","article-title":"Randomized Clinical Trial of Conventional versus Indoor Daylight Photodynamic Therapy for Treatment of Actinic Cheilitis","volume":"12","author":"Arisi","year":"2022","journal-title":"Dermatol. Ther."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Wu, L., Pei, X., Mei, M., Li, Z., and Lu, S. (2022). Study on Photocatalytic Performance of Ag\/TiO(2) Modified Cement Mortar. Materials, 15.","DOI":"10.3390\/ma15114031"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"17662","DOI":"10.1038\/s41598-017-17559-9","article-title":"Ag-doping regulates the cytotoxicity of TiO(2) nanoparticles via oxidative stress in human cancer cells","volume":"7","author":"Ahamed","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"19433","DOI":"10.1038\/srep19433","article-title":"Bonding and Anti-bonding Modes of Plasmon Coupling Effects in TiO2-Ag Core-shell Dimers","volume":"6","author":"Li","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"44638","DOI":"10.1007\/s11356-021-14996-y","article-title":"Recent progress on Ag\/TiO(2) photocatalysts: Photocatalytic and bactericidal behaviors","volume":"28","author":"Chakhtouna","year":"2021","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2553","DOI":"10.2147\/IJN.S130753","article-title":"Cuprous oxide nanoparticle-inhibited melanoma progress by targeting melanoma stem cells","volume":"12","author":"Yu","year":"2017","journal-title":"Int. J. Nanomed."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Elegbeleye, I.F., Maluta, N.E., and Maphanga, R.R. (2021). Density Functional Theory Study of Optical and Electronic Properties of (TiO2) (n = 5,8,68) Clusters for Application in Solar Cells. Molecules, 26.","DOI":"10.3390\/molecules26040955"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2608","DOI":"10.1039\/D2NA00140C","article-title":"Review on LSPR assisted photocatalysis: Effects of physical fields and opportunities in multifield decoupling","volume":"4","author":"Lv","year":"2022","journal-title":"Nanoscale Adv."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1007\/s11671-009-9511-7","article-title":"Accurate Computation of Electric Field Enhancement Factors for Metallic Nanoparticles Using the Discrete Dipole Approximation","volume":"5","author":"DePrince","year":"2010","journal-title":"Nanoscale Res. Lett."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/8\/7061\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:13:55Z","timestamp":1760123635000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/8\/7061"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,11]]},"references-count":49,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["ijms24087061"],"URL":"https:\/\/doi.org\/10.3390\/ijms24087061","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2023,4,11]]}}}