{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:48:31Z","timestamp":1787028511700,"version":"build-2736575974"},"reference-count":57,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,15]],"date-time":"2023-04-15T00:00:00Z","timestamp":1681516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL-139047, DK116591 and AR-71789"],"award-info":[{"award-number":["HL-139047, DK116591 and AR-71789"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Renal denervation (RDN) protects against hypertension, hypertrophy, and heart failure (HF); however, it is not clear whether RDN preserves ejection fraction (EF) during heart failure (HFpEF). To test this hypothesis, we simulated a chronic congestive cardiopulmonary heart failure (CHF) phenotype by creating an aorta-vena cava fistula (AVF) in the C57BL\/6J wild type (WT) mice. Briefly, there are four ways to create an experimental CHF: (1) myocardial infarction (MI), which is basically ligating the coronary artery by instrumenting and injuring the heart; (2) trans-aortic constriction (TAC) method, which mimics the systematic hypertension, but again constricts the aorta on top of the heart and, in fact, exposes the heart; (3) acquired CHF condition, promoted by dietary factors, diabetes, salt, diet, etc., but is multifactorial in nature; and finally, (4) the AVF, which remains the only one wherein AVF is created ~1 cm below the kidneys in which the aorta and vena cava share the common middle-wall. By creating the AVF fistula, the red blood contents enter the vena cava without an injury to the cardiac tissue. This model mimics or simulates the CHF phenotype, for example, during aging wherein with advancing age, the preload volume keeps increasing beyond the level that the aging heart can pump out due to the weakened cardiac myocytes. Furthermore, this procedure also involves the right ventricle to lung to left ventricle flow, thus creating an ideal condition for congestion. The heart in AVF transitions from preserved to reduced EF (i.e., HFpEF to HFrEF). In fact, there are more models of volume overload, such as the pacing-induced and mitral valve regurgitation, but these are also injurious models in nature. Our laboratory is one of the first laboratories to create and study the AVF phenotype in the animals. The RDN was created by treating the cleaned bilateral renal artery. After 6 weeks, blood, heart, and renal samples were analyzed for exosome, cardiac regeneration markers, and the renal cortex proteinases. Cardiac function was analyzed by echocardiogram (ECHO) procedure. The fibrosis was analyzed with a trichrome staining method. The results suggested that there was a robust increase in the exosomes\u2019 level in AVF blood, suggesting a compensatory systemic response during AVF-CHF. During AVF, there was no change in the cardiac eNOS, Wnt1, or \u03b2-catenin; however, during RDN, there were robust increases in the levels of eNOS, Wnt1, and \u03b2-catenin compared to the sham group. As expected in HFpEF, there was perivascular fibrosis, hypertrophy, and pEF. Interestingly, increased levels of eNOS suggested that despite fibrosis, the NO generation was higher and that it most likely contributed to pEF during HF. The RDN intervention revealed an increase in renal cortical caspase 8 and a decrease in caspase 9. Since caspase 8 is protective and caspase 9 is apoptotic, we suggest that RDN protects against the renal stress and apoptosis. It should be noted that others have demonstrated a role of vascular endothelium in preserving the ejection by cell therapy intervention. In the light of foregoing evidence, our findings also suggest that RDN is cardioprotective during HFpEF via preservation of the eNOS and accompanied endocardial-endothelial function.<\/jats:p>","DOI":"10.3390\/ijms24087302","type":"journal-article","created":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T03:14:09Z","timestamp":1681701249000},"page":"7302","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Renal Denervation Helps Preserve the Ejection Fraction by Preserving Endocardial-Endothelial Function during Heart Failure"],"prefix":"10.3390","volume":"24","author":[{"given":"Sathnur","family":"Pushpakumar","sequence":"first","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Louisville, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2415-3314","authenticated-orcid":false,"given":"Mahavir","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Louisville, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Louisville, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluwaseun E.","family":"Akinterinwa","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Louisville, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sri Prakash L.","family":"Mokshagundam","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Metabolism and Diabetes and Robley Rex VA Medical Center, University of Louisville School of Medicine, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Utpal","family":"Sen","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Louisville, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5254-4067","authenticated-orcid":false,"given":"Dinesh K.","family":"Kalra","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, University of Louisville School of Medicine, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh C.","family":"Tyagi","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Louisville, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1016\/0735-1097(95)00381-9","article-title":"Prevalence, clinical features, and prognosis of diastolic heart failure: An epidemiologic perspective","volume":"26","author":"Vasan","year":"1995","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1046\/j.1365-2796.1997.129150000.x","article-title":"Congestive heart failure in old age: Prevalence, mechanisms and 4-year prognosis in the Helsinki Ageing Study","volume":"241","author":"Kupari","year":"1997","journal-title":"J. Intern. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1016\/S0735-1097(99)00118-7","article-title":"Congestive heart failure in subjects with normal versus reduced left ventricular ejection fraction: Prevalence and mortality in a population-based cohort","volume":"33","author":"Vasan","year":"1999","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1016\/S0002-9149(00)01165-6","article-title":"Congestive heart failure despite normal left ventricular systolic function in a population-based sample: The Strong Heart Study","volume":"86","author":"Devereux","year":"2000","journal-title":"Am. J. Cardiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/S0002-9149(00)01393-X","article-title":"Importance of heart failure with preserved systolic function in patients \u226565 years of age","volume":"87","author":"Kitzman","year":"2001","journal-title":"Am. J. Cardiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"631","DOI":"10.7326\/0003-4819-137-8-200210150-00006","article-title":"Outcome of Congestive Heart Failure in Elderly Persons: Influence of Left Ventricular Systolic Function: The Cardiovascular Health Study","volume":"137","author":"Gottdiener","year":"2002","journal-title":"Ann. Intern. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1093\/eurheartj\/ehm554","article-title":"Prognosis of heart failure with preserved ejection fraction: A 5-year prospective population-based study","volume":"29","author":"Tribouilloy","year":"2007","journal-title":"Eur. Heart J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1016\/j.ehj.2004.06.006","article-title":"Differences between patients with a preserved and a depressed left ventricular function: A report from the EuroHeart Failure Survey","volume":"25","author":"Lenzen","year":"2004","journal-title":"Eur. Heart J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.jacc.2005.09.022","article-title":"Clinical presentation, management, and in-hospital outcomes of patients admitted with acute decompensated heart failure with preserved systolic function: A report from the Acute Decompensated Heart Failure National Registry (ADHERE) Database","volume":"47","author":"Yancy","year":"2006","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1016\/j.jacc.2007.04.064","article-title":"Characteristics, treatments, and outcomes of patients with preserved systolic function hospitalized for heart failure: A report from the OPTIMIZE-HF Registry","volume":"50","author":"Fonarow","year":"2007","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1056\/NEJMoa052256","article-title":"Trends in Prevalence and Outcome of Heart Failure with Preserved Ejection Fraction","volume":"355","author":"Owan","year":"2006","journal-title":"N. Engl. J. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1161\/CIRCRESAHA.115.302922","article-title":"Heart failure with preserved ejection fraction: Mechanisms, clinical features, and therapies","volume":"115","author":"Sharma","year":"2014","journal-title":"Circ. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"H1033","DOI":"10.1152\/ajpheart.00680.2017","article-title":"Coronary microvascular dysfunction in patients with heart failure with preserved ejection fraction","volume":"314","author":"Dryer","year":"2018","journal-title":"Am. J. Physiol. Circ. Physiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1161\/CIRCULATIONAHA.119.041886","article-title":"Research Priorities for Heart Failure with Preserved Ejection Fraction: National Heart, Lung, and Blood Institute Working Group Summary","volume":"141","author":"Shah","year":"2020","journal-title":"Circulation"},{"key":"ref_15","first-page":"678","article-title":"Temporal Trends in the Incidence of and Mortality Associated with Heart Failure with Preserved and Reduced Ejection Fraction","volume":"6","author":"Tsao","year":"2018","journal-title":"JACC: Heart Fail."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e012190","DOI":"10.1161\/JAHA.119.012190","article-title":"Impact of Sex on Ventricular-Vascular Stiffness and Long-Term Outcomes in Heart Failure with Preserved Ejection Fraction: TOPCAT Trial Substudy","volume":"8","author":"Beale","year":"2019","journal-title":"J. Am. Heart Assoc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e003330","DOI":"10.1161\/JAHA.116.003330","article-title":"Sex- and Race-Related Differences in Characteristics and Outcomes of Hospitalizations for Heart Failure with Preserved Ejection Fraction","volume":"6","author":"Goyal","year":"2017","journal-title":"J. Am. Heart Assoc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e014669","DOI":"10.1161\/JAHA.119.014669","article-title":"Predictors of Mortality by Sex and Race in Heart Failure with Preserved Ejection Fraction: ARIC Community Surveillance Study","volume":"9","author":"Sharma","year":"2020","journal-title":"J. Am. Heart Assoc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e018574","DOI":"10.1161\/JAHA.120.018574","article-title":"Sex Differences in Heart Failure with Preserved Ejection Fraction","volume":"10","author":"Sotomi","year":"2021","journal-title":"J. Am. Heart Assoc."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.1161\/CIRCRESAHA.119.313572","article-title":"Heart Failure with Preserved Ejection Fraction In Perspective","volume":"124","author":"Pfeffer","year":"2019","journal-title":"Circ. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.jacc.2014.10.031","article-title":"Remote ischemic conditioning","volume":"65","author":"Heusch","year":"2015","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2416","DOI":"10.1093\/cvr\/cvab009","article-title":"Reciprocal organ interactions during heart failure: A position paper from the ESC Working Group on Myocardial Function","volume":"117","author":"Ciccarelli","year":"2021","journal-title":"Cardiovasc. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1002\/ejhf.2209","article-title":"Surgical ablation of the right greater splanchnic nerve for the treatment of heart failure with preserved ejection fraction: First-in-human clinical trial","volume":"23","author":"Gajewski","year":"2021","journal-title":"Eur. J. Heart Fail."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1002\/ehf2.13067","article-title":"Myocardial expression of somatotropic axis, adrenergic signalling, and calcium handling genes in heart failure with preserved ejection fraction and heart failure with reduced ejection fraction","volume":"8","author":"Arcopinto","year":"2021","journal-title":"ESC Heart Fail."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"102862","DOI":"10.1016\/j.ebiom.2020.102862","article-title":"Hematopoietic stem-cell senescence and myocardial repair\u2014Coronary artery disease genotype\/phenotype analysis of post-MI myocardial regeneration response induced by CABG\/CD133+ bone marrow hematopoietic stem cell treatment in RCT PERFECT Phase 3","volume":"57","author":"Wolfien","year":"2020","journal-title":"Ebiomedicine"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"16042","DOI":"10.1073\/pnas.1514250112","article-title":"Musclin is an activity-stimulated myokine that enhances physical endurance","volume":"112","author":"Subbotina","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"745328","DOI":"10.3389\/fphys.2021.745328","article-title":"Remote Hind-Limb Ischemia Mechanism of Preserved Ejection Fraction During Heart Failure","volume":"12","author":"Homme","year":"2021","journal-title":"Front. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"69","DOI":"10.31083\/j.rcm2302069","article-title":"Interrelation between heart failure with preserved ejection fraction and renal impairment","volume":"23","author":"Joslin","year":"2022","journal-title":"Rev. Cardiovasc. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1172\/JCI114941","article-title":"Role of intact cardiac nerves and reflex mechanisms in desensitization to catecholamines in conscious dogs","volume":"86","author":"Nejima","year":"1990","journal-title":"J. Clin. Investig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"R248","DOI":"10.1152\/ajpregu.00490.2013","article-title":"Postmenopausal hypertension: Role of the sympathetic nervous system in an animal model","volume":"306","author":"Maranon","year":"2014","journal-title":"Am. J. Physiol. Integr. Comp. Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0014-2999(01)01542-4","article-title":"Renal effects of glucagon-like peptide in rats","volume":"434","author":"Moreno","year":"2001","journal-title":"Eur. J. Pharmacol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1681\/ASN.2021060757","article-title":"Angiopoietins as Prognostic Markers for Future Kidney Disease and Heart Failure Events after Acute Kidney Injury","volume":"33","author":"Mansour","year":"2022","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1159\/000522377","article-title":"Congenital Heart Disease in Adults with Autosomal Dominant Polycystic Kidney Disease","volume":"53","author":"Chedid","year":"2022","journal-title":"Am. J. Nephrol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"F589","DOI":"10.1152\/ajprenal.00372.2021","article-title":"Mitochondria as mediators of systemic inflammation and organ cross talk in acute kidney injury","volume":"322","author":"Hepokoski","year":"2022","journal-title":"Am. J. Physiol. Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5204","DOI":"10.1074\/jbc.H120.013321","article-title":"Learning the ABCs of ATP release","volume":"295","author":"Libby","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"H892","DOI":"10.1152\/ajpheart.00287.2022","article-title":"Cell therapy attenuates endothelial dysfunction in hypertensive rats with heart failure and preserved ejection fraction","volume":"323","author":"Mesquita","year":"2022","journal-title":"Am. J. Physiol. Circ. Physiol."},{"key":"ref_37","first-page":"563","article-title":"Mechanisms of disease: Erythropoietin\u2014An old hormone with a new mission? Nature clinical practice","volume":"3","author":"Fliser","year":"2006","journal-title":"Cardiovasc. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2919","DOI":"10.1007\/s11255-022-03207-3","article-title":"Evaluation of eryptosis in patients with chronic kidney disease","volume":"54","author":"Gok","year":"2022","journal-title":"Int. Urol. Nephrol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"465","DOI":"10.31128\/AJGP-03-19-4873","article-title":"Heart failure with preserved ejection fraction: A growing global epidemic","volume":"48","author":"Naing","year":"2019","journal-title":"Aust. J. Gen. Pract."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"12888","DOI":"10.1038\/s41598-017-13105-9","article-title":"Denervation-related alterations and biological activity of miRNAs contained in exosomes released by skeletal muscle fibers","volume":"7","author":"Hamidi","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1111\/apha.12793","article-title":"Beneficial effects of renal denervation on cardiac angiogenesis in rats with prolonged pressure overload","volume":"220","author":"Lu","year":"2016","journal-title":"Acta Physiol."},{"key":"ref_42","first-page":"1609","article-title":"[Renal sympathetic denervation can significantly reduce blood pressure and improve arterial stiffness in hypertensive beagles]","volume":"41","author":"Ye","year":"2021","journal-title":"Nan Fang Yi Ke Da Xue Xue Bao = J. South. Med. Univ."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1007\/s00592-020-01532-6","article-title":"Renal denervation improves vascular endothelial dysfunction by inducing autophagy via AMPK\/mTOR signaling activation in a rat model of type 2 diabetes mellitus with insulin resistance","volume":"57","author":"Wang","year":"2020","journal-title":"Acta Diabetol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.ijcard.2015.04.041","article-title":"The effect of renal denervation on endothelial function and inflammatory markers in patients with resistant hypertension","volume":"188","author":"Eikelis","year":"2015","journal-title":"Int. J. Cardiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e001353","DOI":"10.1161\/JAHA.114.001353","article-title":"Potential Reduction of Interstitial Myocardial Fibrosis with Renal Denervation","volume":"3","author":"Doltra","year":"2014","journal-title":"J. Am. Heart Assoc."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1080\/10641963.2020.1833022","article-title":"Renal sympathetic denervation attenuates left ventricle hypertrophy in spontaneously hypertensive rats by suppressing the Raf\/MEK\/ERK signaling pathway","volume":"43","author":"Xiao","year":"2020","journal-title":"Clin. Exp. Hypertens."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.1161\/CIR.0b013e31829e8807","article-title":"2013 ACCF\/AHA guideline for the management of heart failure: Executive summary: A report of the American College of Cardiology Foundation\/American Heart Association Task Force on practice guidelines","volume":"128","author":"Yancy","year":"2013","journal-title":"Circulation"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1093\/eurheartj\/ehw128","article-title":"2016 ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure: The Task Force for the diagnosis and treatment of acute and chronic heart failure of the European Society of Cardiology (ESC)Developed with the special contribution of the Heart Failure Association (HFA) of the ESC","volume":"37","author":"Ponikowski","year":"2016","journal-title":"Eur. Heart J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e007312","DOI":"10.1161\/JAHA.117.007312","article-title":"Impact of Renal Denervation on Atrial Arrhythmogenic Substrate in Ischemic Model of Heart Failure","volume":"7","author":"Yamada","year":"2018","journal-title":"J. Am. Heart Assoc."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1152\/ajplegacy.1961.200.6.1157","article-title":"Compensations of cardiac output and other circulatory functions in areflex dogs with large A-V fistulas","volume":"200","author":"Guyton","year":"1961","journal-title":"Am. J. Physiol. Content"},{"key":"ref_51","first-page":"H2071","article-title":"Temporal evaluation of left ventricular remodeling and function in rats with chronic volume overload","volume":"271","author":"Brower","year":"1996","journal-title":"Am. J. Physiol. Content"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"H1197","DOI":"10.1152\/ajpheart.00483.2001","article-title":"Apoptosis in the left ventricle of chronic volume overload causes endocardial endothelial dysfunction in rats","volume":"282","author":"Cox","year":"2002","journal-title":"Am. J. Physiol. Circ. Physiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1006\/jmcc.1996.0132","article-title":"Post-transcriptional Regulation of Extracellular Matrix Metalloproteinase in Human Heart End-stage Failure Secondary to Ischemic Cardiomyopathy","volume":"28","author":"Tyagi","year":"1996","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_54","first-page":"352","article-title":"Sustained BP reduction with renal denervation","volume":"19","author":"Lim","year":"2022","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1002\/(SICI)1097-4644(19961101)63:2<185::AID-JCB6>3.0.CO;2-U","article-title":"Differential gene expression of extracellular matrix components in dilated cardiomyopathy","volume":"63","author":"Tyagi","year":"1996","journal-title":"J. Cell. Biochem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1002\/jcb.20581","article-title":"Reduced alpha adrenergic mediated contraction of renal preglomerular blood vessels as a function of gender and aging","volume":"96","author":"Passmore","year":"2005","journal-title":"J. Cell. Biochem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"515","DOI":"10.2307\/2531456","article-title":"A modified Bonferroni method for discrete data","volume":"46","author":"Tarone","year":"1990","journal-title":"Biometrics"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/8\/7302\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:16:16Z","timestamp":1760123776000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/8\/7302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,15]]},"references-count":57,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["ijms24087302"],"URL":"https:\/\/doi.org\/10.3390\/ijms24087302","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,15]]}}}