{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:04:44Z","timestamp":1760148284644,"version":"build-2065373602"},"reference-count":42,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,16]],"date-time":"2023-04-16T00:00:00Z","timestamp":1681603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"InFlectis BioScience an Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-15-CE14-0004-02","ANR: 20-ASTC-0032-01-hErOiSmE\/Agence Nationale de la Recherche"],"award-info":[{"award-number":["ANR-15-CE14-0004-02","ANR: 20-ASTC-0032-01-hErOiSmE\/Agence Nationale de la Recherche"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"name":"ANR heroism","award":["ANR-15-CE14-0004-02","ANR: 20-ASTC-0032-01-hErOiSmE\/Agence Nationale de la Recherche"],"award-info":[{"award-number":["ANR-15-CE14-0004-02","ANR: 20-ASTC-0032-01-hErOiSmE\/Agence Nationale de la Recherche"]}]},{"name":"the F\u00e9d\u00e9ration Fran\u00e7aise de Cardiologie and the association Sauve ton Coeur","award":["ANR-15-CE14-0004-02","ANR: 20-ASTC-0032-01-hErOiSmE\/Agence Nationale de la Recherche"],"award-info":[{"award-number":["ANR-15-CE14-0004-02","ANR: 20-ASTC-0032-01-hErOiSmE\/Agence Nationale de la Recherche"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In clinical practice, extracorporeal circulation (ECC) is associated with coagulopathy and inflammation, eventually leading to organ injuries without preventive systemic pharmacological treatment. Relevant models are needed to reproduce the pathophysiology observed in humans and preclinical tests. Rodent models are less expensive than large models but require adaptations and validated comparisons to clinics. This study aimed to develop a rat ECC model and to establish its clinical relevance. One hour of veno-arterial ECC or a sham procedure were achieved on mechanically ventilated rats after cannulations with a mean arterial pressure objective &gt; 60 mmHg. Five hours post-surgery, the rats\u2019 behavior, plasmatic\/blood biomarkers, and hemodynamics were measured. Blood biomarkers and transcriptomic changes were compared in 41 patients undergoing on-pump cardiac surgery. Five hours post-ECC, the rats presented hypotension, hyperlactatemia, and behavioral alterations. The same patterns of marker measurements (Lactate dehydrogenase, Creatinine kinase, ASAT, ALAT, and Troponin T) were observed in both rats and human patients. Transcriptome analyses showed similarity in both humans and rats in the biological processes involved in the ECC response. This new ECC rat model seems to resemble both ECC clinical procedures and the associated pathophysiology, but with early organ injury corresponding to a severe phenotype. Although the mechanisms at stake in the post-ECC pathophysiology of rats or humans need to be described, this new rat model appears to be a relevant and costless preclinical model of human ECC.<\/jats:p>","DOI":"10.3390\/ijms24087338","type":"journal-article","created":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T03:14:09Z","timestamp":1681701249000},"page":"7338","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Rat Model of Clinically Relevant Extracorporeal Circulation Develops Early Organ Dysfunctions"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4722-9422","authenticated-orcid":false,"given":"Antoine","family":"Persello","sequence":"first","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, CNRS, INSERM, l\u2019institut du thorax, F-44000 Nantes, France"},{"name":"InFlectis BioScience, 44200 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fouzia","family":"Souab","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, CNRS, INSERM, l\u2019institut du thorax, F-44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8827-3526","authenticated-orcid":false,"given":"Thomas","family":"Dupas","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, CNRS, INSERM, l\u2019institut du thorax, F-44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginie","family":"Aillerie","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, CNRS, INSERM, l\u2019institut du thorax, F-44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edith","family":"Bigot","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, CHU de Nantes, 44800 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manon","family":"Denis","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, CNRS, INSERM, l\u2019institut du thorax, F-44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5962-215X","authenticated-orcid":false,"given":"Ang\u00e9lique","family":"Erraud","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, CNRS, INSERM, l\u2019institut du thorax, F-44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Pel\u00e9","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, CNRS, INSERM, l\u2019institut du thorax, F-44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ang\u00e9lique","family":"Blangy-Letheule","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, CNRS, INSERM, l\u2019institut du thorax, F-44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Miniou","sequence":"additional","affiliation":[{"name":"InFlectis BioScience, 44200 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7214-1414","authenticated-orcid":false,"given":"Philippe","family":"Guedat","sequence":"additional","affiliation":[{"name":"InFlectis BioScience, 44200 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2782-9615","authenticated-orcid":false,"given":"Michel","family":"De Waard","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, CNRS, INSERM, l\u2019institut du thorax, F-44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuelle","family":"Abgueguen","sequence":"additional","affiliation":[{"name":"InFlectis 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