{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T01:16:54Z","timestamp":1782523014107,"version":"3.54.5"},"reference-count":54,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T00:00:00Z","timestamp":1682035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Scholarship Council","award":["OCENW.KLEIN.558"],"award-info":[{"award-number":["OCENW.KLEIN.558"]}]},{"name":"NWO","award":["OCENW.KLEIN.558"],"award-info":[{"award-number":["OCENW.KLEIN.558"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The pentameric \u03b3-Aminobutyric acid type A receptors (GABAARs) are ligand-gated ion channels that mediate the majority of inhibitory neurotransmission in the brain. In the cerebellum, the two main receptor subtypes are the 2\u03b11\/2\u03b2\/\u03b3 and 2\u03b16\/2\u03b2\/\u03b4 subunits. In the present study, an interaction proteomics workflow was used to reveal additional subtypes that contain both \u03b11 and \u03b16 subunits. Immunoprecipitation of the \u03b16 subunit from mouse brain cerebellar extract co-purified the \u03b11 subunit. In line with this, pre-incubation of the cerebellar extract with anti-\u03b16 antibodies and analysis by blue native gel electrophoresis mass-shifted part of the \u03b11 complexes, indicative of the existence of an \u03b11\u03b16-containing receptor. Subsequent mass spectrometry of the blue native gel showed the \u03b11\u03b16-containing receptor subtype to exist in two main forms, i.e., with or without Neuroligin-2. Immunocytochemistry on a cerebellar granule cell culture revealed co-localization of \u03b16 and \u03b11 in post-synaptic puncta that apposed the presynaptic marker protein Vesicular GABA transporter, indicative of the presence of this synaptic GABAAR subtype.<\/jats:p>","DOI":"10.3390\/ijms24087632","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T04:48:11Z","timestamp":1682052491000},"page":"7632","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Blue Native PAGE\u2013Antibody Shift in Conjunction with Mass Spectrometry to Reveal Protein Subcomplexes: Detection of a Cerebellar \u03b11\/\u03b16-Subunits Containing \u03b3-Aminobutyric Acid Type A Receptor Subtype"],"prefix":"10.3390","volume":"24","author":[{"given":"Miao","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4973-5732","authenticated-orcid":false,"given":"Frank","family":"Koopmans","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iryna","family":"Paliukhovich","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie J. F.","family":"van der Spek","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"August B.","family":"Smit","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6983-5055","authenticated-orcid":false,"given":"Ka Wan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10682","DOI":"10.1038\/ncomms10682","article-title":"Shisa6 Traps AMPA Receptors at Postsynaptic Sites and Prevents Their Desensitization during Synaptic Activity","volume":"7","author":"Klaassen","year":"2016","journal-title":"Nat. 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