{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T08:04:21Z","timestamp":1777104261564,"version":"3.51.4"},"reference-count":42,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T00:00:00Z","timestamp":1682294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministerium f\u00fcr Wissenschaft, Forschung und Kunst Baden-W\u00fcrttemberg","award":["K5272\/3-1"],"award-info":[{"award-number":["K5272\/3-1"]}]},{"name":"Ministerium f\u00fcr Wissenschaft, Forschung und Kunst Baden-W\u00fcrttemberg","award":["80205PHJUS"],"award-info":[{"award-number":["80205PHJUS"]}]},{"name":"European Social Fund","award":["K5272\/3-1"],"award-info":[{"award-number":["K5272\/3-1"]}]},{"name":"European Social Fund","award":["80205PHJUS"],"award-info":[{"award-number":["80205PHJUS"]}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["K5272\/3-1"],"award-info":[{"award-number":["K5272\/3-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["80205PHJUS"],"award-info":[{"award-number":["80205PHJUS"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009139","name":"German Center for Infection Research","doi-asserted-by":"publisher","award":["K5272\/3-1"],"award-info":[{"award-number":["K5272\/3-1"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009139","name":"German Center for Infection Research","doi-asserted-by":"publisher","award":["80205PHJUS"],"award-info":[{"award-number":["80205PHJUS"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]},{"name":"J\u00fcrgen Manchot Foundation","award":["K5272\/3-1"],"award-info":[{"award-number":["K5272\/3-1"]}]},{"name":"J\u00fcrgen Manchot Foundation","award":["80205PHJUS"],"award-info":[{"award-number":["80205PHJUS"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Newborns and especially preterm infants are much more susceptible to infections than adults. Due to immature adaptive immunity, especially innate immune cells play an important role in a newborn\u2019s infection defense. Neonatal neutrophils exhibit profound differences in their functionality compared to neutrophils of adults. In particular, neonates possess a relevant population of suppressive neutrophils, which not only inhibit but also specifically modulate the function of T-cells. In this study, we investigated whether neonatal neutrophils are already involved in T-cell development in the thymus. For this purpose, we used a newly developed model of antibody-mediated immune cell depletion in which we administered a depleting antibody to pregnant and then lactating dams. Using this method, we were able to sufficiently deplete Ly6G-positive neutrophils in offspring. We demonstrated that the depletion of neutrophils in newborn mice resulted in altered peripheral T-cell homeostasis with a decreased CD4+\/CD8+ T-cell ratio and decreased expression of CD62L. Neutrophil depletion even affected T-cell development in the thymus, with increased double positive thymocytes and a decreased CD4+\/CD8+ single positive thymocyte ratio. Altogether, we demonstrated a previously unknown mechanism mediating neutrophils\u2019 immunomodulatory effects in newborns.<\/jats:p>","DOI":"10.3390\/ijms24097763","type":"journal-article","created":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T06:20:42Z","timestamp":1682317242000},"page":"7763","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Depletion of Ly6G-Expressing Neutrophilic Cells Leads to Altered Peripheral T-Cell Homeostasis and Thymic Development in Neonatal Mice"],"prefix":"10.3390","volume":"24","author":[{"given":"Jessica","family":"R\u00fchle","sequence":"first","affiliation":[{"name":"Department of Neonatology, University of T\u00fcbingen, D-72076 T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Ginzel","sequence":"additional","affiliation":[{"name":"Department of Pediatric and Adolescent Surgery, Paracelsus Medical University Hospital, A-5020 Salzburg, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Dietz","sequence":"additional","affiliation":[{"name":"Department of Neonatology, University of T\u00fcbingen, D-72076 T\u00fcbingen, Germany"},{"name":"Department of Neonatology, Heidelberg University Children\u2019s Hospital, D-69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Department of Neonatology, University of T\u00fcbingen, D-72076 T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2584-589X","authenticated-orcid":false,"given":"Trim","family":"Lajqi","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Heidelberg University Children\u2019s Hospital, D-69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Beer-Hammer","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Experimental Therapy and Toxicology, Institute of Experimental and Clinical Pharmacology and Pharmacogenomic and ICePhA, University of T\u00fcbingen, D-72074 T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1072-0066","authenticated-orcid":false,"given":"Christian F.","family":"Poets","sequence":"additional","affiliation":[{"name":"Department of Neonatology, University of T\u00fcbingen, D-72076 T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Gille","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Heidelberg University Children\u2019s Hospital, D-69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3718-5507","authenticated-orcid":false,"given":"Natascha","family":"K\u00f6stlin-Gille","sequence":"additional","affiliation":[{"name":"Department of Neonatology, University of T\u00fcbingen, D-72076 T\u00fcbingen, Germany"},{"name":"Department of Neonatology, Heidelberg University Children\u2019s Hospital, D-69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.1016\/S0140-6736(17)31002-4","article-title":"Neonatal sepsis","volume":"390","author":"Shane","year":"2017","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"F257","DOI":"10.1136\/archdischild-2014-306213","article-title":"Late-onset neonatal sepsis: Recent developments","volume":"100","author":"Dong","year":"2015","journal-title":"Arch. 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