{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:39:35Z","timestamp":1783489175067,"version":"3.55.0"},"reference-count":53,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,4,25]],"date-time":"2023-04-25T00:00:00Z","timestamp":1682380800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017607","name":"Shenzhen Fundamental Research Program","doi-asserted-by":"publisher","award":["JCYJ20180306173803232"],"award-info":[{"award-number":["JCYJ20180306173803232"]}],"id":[{"id":"10.13039\/501100017607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017607","name":"Shenzhen Fundamental Research Program","doi-asserted-by":"publisher","award":["cstc2020jcyj-msxmX0186"],"award-info":[{"award-number":["cstc2020jcyj-msxmX0186"]}],"id":[{"id":"10.13039\/501100017607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017607","name":"Shenzhen Fundamental Research Program","doi-asserted-by":"publisher","award":["GJHZ20200731095006018"],"award-info":[{"award-number":["GJHZ20200731095006018"]}],"id":[{"id":"10.13039\/501100017607","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Chongqing, China","award":["JCYJ20180306173803232"],"award-info":[{"award-number":["JCYJ20180306173803232"]}]},{"name":"Natural Science Foundation of Chongqing, China","award":["cstc2020jcyj-msxmX0186"],"award-info":[{"award-number":["cstc2020jcyj-msxmX0186"]}]},{"name":"Natural Science Foundation of Chongqing, China","award":["GJHZ20200731095006018"],"award-info":[{"award-number":["GJHZ20200731095006018"]}]},{"name":"Science, Technology, and Innovation Commission of Shenzhen","award":["JCYJ20180306173803232"],"award-info":[{"award-number":["JCYJ20180306173803232"]}]},{"name":"Science, Technology, and Innovation Commission of Shenzhen","award":["cstc2020jcyj-msxmX0186"],"award-info":[{"award-number":["cstc2020jcyj-msxmX0186"]}]},{"name":"Science, Technology, and Innovation Commission of Shenzhen","award":["GJHZ20200731095006018"],"award-info":[{"award-number":["GJHZ20200731095006018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Epididymitis is an epididymal inflammation that may lead to male infertility. Dendritic cells (DCs) and myeloid differentiation primary response gene 88 (Myd88) were associated with epididymitis in rodents. However, the functions of Myd88 on epididymal DCs remain unclear. This study investigated the role of Myd88 in DCs for epididymitis. The Myd88 signaling pathway, phenotypes of DC subsets, and cytokines were investigated in lipopolysaccharide (LPS)-induced epididymitis in mice. CRISPR-Cas9 was used to knockout Myd88 in bone-marrow-derived dendritic cells (BMDCs) and immortalized mouse epididymal (DC2) cell line. In the vivo experiments, levels of the proinflammatory cytokines IL-1\u03b1, IL-6, IL-17A, TNF-\u03b1, IL-1\u03b2, MCP-1, and GM-CSF, mRNA for MyD88 related genes, and the percentages of monocyte-derived DCs (Mo-DCs) were significantly elevated in mice with epididymitis. In the vitro experiments, LPS significantly promoted the apoptosis of BMDCs. In addition, the concentration of inflammatory cytokines in BMDCs and DC2s were increased in the LPS group, while decreasing after the knockout of Myd88. These findings indicate that Myd88 on DCs is involved in the inflammation of epididymitis in mice, which may be a potential target for better strategies regarding the treatment of immunological male infertility.<\/jats:p>","DOI":"10.3390\/ijms24097838","type":"journal-article","created":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T02:30:52Z","timestamp":1682476252000},"page":"7838","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Myd88 Signaling Is Involved in the Inflammatory Response in LPS-Induced Mouse Epididymitis and Bone-Marrow-Derived Dendritic Cells"],"prefix":"10.3390","volume":"24","author":[{"given":"Jin-Chuan","family":"Liu","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China"},{"name":"Department of Obstetrics and Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology, Daping Hospital, Army Medical University, Chongqing 400042, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun-Xiong","family":"Zeng","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China"},{"name":"Department of Obstetrics and Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Yang","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minmin","family":"Lyu","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology, Daping Hospital, Army Medical University, Chongqing 400042, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William Shu-Biu","family":"Yeung","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China"},{"name":"Department of Obstetrics and Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip Chi-Ngong","family":"Chiu","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China"},{"name":"Department of Obstetrics and Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Haidl","sequence":"additional","affiliation":[{"name":"Department of Andrology, Bonn University Hospital, Campus-Venusberg 1, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Allam","sequence":"additional","affiliation":[{"name":"Department of Andrology, Bonn University Hospital, Campus-Venusberg 1, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5350-892X","authenticated-orcid":false,"given":"Yong-Gang","family":"Duan","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S770","DOI":"10.1093\/cid\/civ812","article-title":"Epididymitis","volume":"61","author":"Taylor","year":"2015","journal-title":"Clin. 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