{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T19:12:07Z","timestamp":1789672327688,"version":"4.0.1"},"reference-count":41,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T00:00:00Z","timestamp":1682726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Science Foundation","award":["18-74-10019"],"award-info":[{"award-number":["18-74-10019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Rapid and reliable techniques for virus identification are required in light of recurring epidemics and pandemics throughout the world. Several techniques have been distributed for testing the flow of patients. Polymerase chain reaction with reverse transcription is a reliable and sensitive, though not rapid, tool. The antibody-based strip is a rapid, though not reliable, and sensitive tool. A set of alternative tools is being developed to meet all the needs of the customer. Surface-enhanced Raman spectroscopy (SERS) provides the possibility of single molecule detection taking several minutes. Here, a multiplex lithographic SERS aptasensor was developed aiming at the detection of several respiratory viruses in one pot within 17 min. The four labeled aptamers were anchored onto the metal surface of four SERS zones; the caught viruses affect the SERS signals of the labels, providing changes in the analytical signals. The sensor was able to decode mixes of SARS-CoV-2 (severe acute respiratory syndrome coronavirus two), influenza A virus, respiratory syncytial virus, and adenovirus within a single experiment through a one-stage recognition process.<\/jats:p>","DOI":"10.3390\/ijms24098081","type":"journal-article","created":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T13:54:46Z","timestamp":1682949286000},"page":"8081","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Multiplex Lithographic SERS Aptasensor for Detection of Several Respiratory Viruses in One Pot"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6731-9508","authenticated-orcid":false,"given":"Vladimir","family":"Kukushkin","sequence":"first","affiliation":[{"name":"Osipyan Institute of Solid State Physics, Russian Academy of Science, 142432 Chernogolovka, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oganes","family":"Ambartsumyan","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexei","family":"Subekin","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Astrakhantseva","sequence":"additional","affiliation":[{"name":"Osipyan Institute of Solid State Physics, Russian Academy of Science, 142432 Chernogolovka, Russia"},{"name":"Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9397-3762","authenticated-orcid":false,"given":"Vladimir","family":"Gushchin","sequence":"additional","affiliation":[{"name":"N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Nikonova","sequence":"additional","affiliation":[{"name":"Mechnikov Research Institute of Vaccines and Sera, 105064 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Dorofeeva","sequence":"additional","affiliation":[{"name":"Mechnikov Research Institute of Vaccines and Sera, 105064 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitaly","family":"Zverev","sequence":"additional","affiliation":[{"name":"Mechnikov Research Institute of Vaccines and Sera, 105064 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Keshek","sequence":"additional","affiliation":[{"name":"Chemistry Department of Lomonosov, Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadezda","family":"Meshcheryakova","sequence":"additional","affiliation":[{"name":"Chemistry Department of Lomonosov, Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Zaborova","sequence":"additional","affiliation":[{"name":"Chemistry Department of Lomonosov, Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1892-0548","authenticated-orcid":false,"given":"Alexandra","family":"Gambaryan","sequence":"additional","affiliation":[{"name":"Chumakov Federal Scientific Center for Research, Development of Immune and Biological Products RAS, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5260-1973","authenticated-orcid":false,"given":"Elena","family":"Zavyalova","sequence":"additional","affiliation":[{"name":"Chemistry Department of Lomonosov, Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,29]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2023, April 05). 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