{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T10:36:53Z","timestamp":1769942213241,"version":"3.49.0"},"reference-count":45,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T00:00:00Z","timestamp":1682985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SPP1656"],"award-info":[{"award-number":["SPP1656"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GL 899\/1-1"],"award-info":[{"award-number":["GL 899\/1-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["TRR241"],"award-info":[{"award-number":["TRR241"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Deutsche Z\u00f6liakie-Gesellschaft e.V.","award":["SPP1656"],"award-info":[{"award-number":["SPP1656"]}]},{"name":"Deutsche Z\u00f6liakie-Gesellschaft e.V.","award":["GL 899\/1-1"],"award-info":[{"award-number":["GL 899\/1-1"]}]},{"name":"Deutsche Z\u00f6liakie-Gesellschaft e.V.","award":["TRR241"],"award-info":[{"award-number":["TRR241"]}]},{"name":"Charit\u00e9 Medizinische Bibliothek","award":["SPP1656"],"award-info":[{"award-number":["SPP1656"]}]},{"name":"Charit\u00e9 Medizinische Bibliothek","award":["GL 899\/1-1"],"award-info":[{"award-number":["GL 899\/1-1"]}]},{"name":"Charit\u00e9 Medizinische Bibliothek","award":["TRR241"],"award-info":[{"award-number":["TRR241"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>To demonstrate and analyze the specific T-cell response following barrier disruption and antigen translocation, circulating food antigen-specific effector T-cells isolated from peripheral blood were analyzed in patients suffering from celiac disease (CeD) as well as inflammatory bowel disease (IBD). We applied the antigen-reactive T-cell enrichment (ARTE) technique allowing for phenotypical and functional flow cytometric analyses of rare nutritional antigen-specific T-cells, including the celiac disease-causing gliadin (gluten). For CeD, patient groups, including treatment-refractory cases, differ significantly from healthy controls. Even symptom-free patients on a gluten-free diet were distinguishable from healthy controls, without being previously challenged with gluten. Moreover, frequency and phenotype of nutritional antigen-specific T-cells of IBD patients directly correlated to the presence of small intestinal inflammation. Specifically, the frequency of antigen specific T-cells as well as pro-inflammatory cytokines was increased in patients with active CeD or Crohn\u2019s disease, respectively. These results suggest active small intestinal inflammation as key for the development of a peripheral food antigen-specific T-cell response in Crohn\u2019s disease and celiac disease.<\/jats:p>","DOI":"10.3390\/ijms24098153","type":"journal-article","created":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T01:36:38Z","timestamp":1683077798000},"page":"8153","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Analysis of Circulating Food Antigen-Specific T-Cells in Celiac Disease and Inflammatory Bowel Disease"],"prefix":"10.3390","volume":"24","author":[{"given":"Yasmina","family":"Rodr\u00edguez-Sillke","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charit\u00e9-University Medicine Berlin, 13125 Berlin, Germany"},{"name":"Institute of Nutrition, University of Potsdam, 14558 Nuthetal, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Schumann","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charit\u00e9-University Medicine Berlin, 13125 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donata","family":"Lissner","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charit\u00e9-University Medicine Berlin, 13125 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9217-8241","authenticated-orcid":false,"given":"Federica","family":"Branchi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charit\u00e9-University Medicine Berlin, 13125 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4306-033X","authenticated-orcid":false,"given":"Fabian","family":"Proft","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charit\u00e9-University Medicine Berlin, 13125 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Steinhoff","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hospital Hygiene, Philipps University of Marburg, 35043 Marburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0055-958X","authenticated-orcid":false,"given":"Britta","family":"Siegmund","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charit\u00e9-University Medicine Berlin, 13125 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rainer","family":"Glauben","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Infectious Diseases, and Rheumatology, Campus Benjamin Franklin, Charit\u00e9-University Medicine Berlin, 13125 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.4049\/jimmunol.1102190","article-title":"Frequency of epitope-specific naive CD4(+) T cells correlates with immunodominance in the human memory repertoire","volume":"188","author":"Kwok","year":"2012","journal-title":"J. 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