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(ISCIII)","award":["PT17\/0019"],"award-info":[{"award-number":["PT17\/0019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Fasciola hepatica is the main causative agent of fasciolosis, a zoonotic parasitic disease of growing public health concern. F. hepatica metacercariae are ingested by the host and excyst in the intestine, thereby releasing the newly excysted juveniles (FhNEJ), which traverse the gut wall and migrate towards the biliary ducts. Since blocking F. hepatica development is challenging after crossing of the intestinal wall, targeting this first step of migration might result in increased therapeutic success. The intestinal extracellular matrix (ECM) is constituted by a network of structural proteins, including laminin (LM) and fibronectin (FN), that provide mechanical support while acting as physical barrier against intestinal pathogens. Here, we employed ELISA and immunofluorescent assays to test for the presence of LM- and FN-binding proteins on a tegument-enriched antigenic fraction of FhNEJ, and further determined their identity by two-dimensional electrophoresis coupled to mass spectrometry. Additionally, we performed enzymatic assays that revealed for the first time the capability of the juvenile-specific cathepsin L3 to degrade LM, and that LM degradation by FhNEJ proteins is further potentiated in the presence of host plasminogen. Finally, a proteomic analysis showed that the interaction with LM triggers protein changes in FhNEJ that may be relevant for parasite growth and adaptation inside the mammalian host. Altogether, our study provides valuable insights into the molecular interplay between FhNEJ and the intestinal ECM, which may lead to the identification of targetable candidates for the development of more effective control strategies against fasciolosis.<\/jats:p>","DOI":"10.3390\/ijms24098165","type":"journal-article","created":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T02:03:18Z","timestamp":1683165798000},"page":"8165","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Molecular Characterization of the Interplay between Fasciola hepatica Juveniles and Laminin as a Mechanism to Adhere to and Break through the Host Intestinal Wall"],"prefix":"10.3390","volume":"24","author":[{"given":"Judit","family":"Serrat","sequence":"first","affiliation":[{"name":"Laboratory of Helminth Parasites of Zoonotic Importance (ATENEA), Institute of Natural Resources and Agrobiology of Salamanca (IRNASA-CSIC), C\/Cordel de Merinas 40-52, 37008 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Torres-Valle","sequence":"additional","affiliation":[{"name":"Laboratory of Helminth Parasites of Zoonotic Importance (ATENEA), Institute of Natural Resources and Agrobiology of Salamanca (IRNASA-CSIC), C\/Cordel de Merinas 40-52, 37008 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7412-4597","authenticated-orcid":false,"given":"Marta","family":"L\u00f3pez-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Laboratory of Helminth Parasites of Zoonotic Importance (ATENEA), Institute of Natural Resources and Agrobiology of Salamanca (IRNASA-CSIC), C\/Cordel de Merinas 40-52, 37008 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Becerro-Recio","sequence":"additional","affiliation":[{"name":"Laboratory of Helminth Parasites of Zoonotic Importance (ATENEA), Institute of Natural Resources and Agrobiology of Salamanca (IRNASA-CSIC), C\/Cordel de Merinas 40-52, 37008 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar","family":"Siles-Lucas","sequence":"additional","affiliation":[{"name":"Laboratory of Helminth Parasites of Zoonotic Importance (ATENEA), Institute of Natural Resources and Agrobiology of Salamanca (IRNASA-CSIC), C\/Cordel de Merinas 40-52, 37008 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4279-4761","authenticated-orcid":false,"given":"Javier","family":"Gonz\u00e1lez-Miguel","sequence":"additional","affiliation":[{"name":"Laboratory of Helminth Parasites of Zoonotic Importance (ATENEA), Institute of Natural Resources and Agrobiology of Salamanca (IRNASA-CSIC), C\/Cordel de Merinas 40-52, 37008 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.rvsc.2020.10.011","article-title":"Fascioliasis and fasciolopsiasis: Current knowledge and future trends","volume":"134","author":"Serrat","year":"2021","journal-title":"Res. Vet. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1016\/j.ijpara.2014.07.011","article-title":"Liver fluke vaccines in ruminants: Strategies, progress and future opportunities","volume":"44","author":"Toet","year":"2014","journal-title":"Int. J. Parasitol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.vetpar.2013.04.005","article-title":"Global food security: The impact of veterinary parasites and parasitologists","volume":"195","author":"Fitzpatrick","year":"2013","journal-title":"Vet. Parasitol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/978-3-030-18616-6_4","article-title":"Fascioliasis","volume":"Volume 1154","author":"Toledo","year":"2019","journal-title":"Digenetic Trematodes"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"786195","DOI":"10.1155\/2015\/786195","article-title":"Fascioliasis: An ongoing zoonotic trematode infection","volume":"2015","author":"Nyindo","year":"2015","journal-title":"Biomed. Res. Int."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.pt.2020.09.007","article-title":"Insights into Fasciola hepatica juveniles: Crossing the fasciolosis Rubicon","volume":"37","year":"2021","journal-title":"Trends. Parasitol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"610189","DOI":"10.3389\/fmed.2021.610189","article-title":"The charming world of the extracellular matrix: A dynamic and protective network of the intestinal wall","volume":"8","author":"Pompili","year":"2021","journal-title":"Front. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1111\/j.1574-6976.2012.00340.x","article-title":"Human pathogens utilize host extracellular matrix proteins laminin and collagen for adhesion and invasion of the host","volume":"36","author":"Singh","year":"2012","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Betanzos, A., Ba\u00f1uelos, C., and Orozco, E. (2019). Host invasion by pathogenic amoebae: Epithelial disruption by parasite proteins. Genes, 10.","DOI":"10.3390\/genes10080618"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1038\/nri3738","article-title":"Regional specialization within the intestinal immune system","volume":"14","author":"Mowat","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/bph.14195","article-title":"The extracellular matrix as a key regulator of intracellular signalling networks","volume":"176","author":"Hastings","year":"2019","journal-title":"Br. J. Pharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.chom.2009.05.011","article-title":"Bacterial adhesins in host-microbe interactions","volume":"5","author":"Kline","year":"2009","journal-title":"Cell Host Microbe"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/j.1365-2141.2005.05444.x","article-title":"Molecular mechanisms of fibrinolysis","volume":"129","author":"Hajjar","year":"2005","journal-title":"Br. J. Haematol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Serrat, J., Becerro-Recio, D., Torres-Valle, M., Sim\u00f3n, F., Valero, M.A., Bargues, M.D., Mas-Coma, S., Siles-Lucas, M., and Gonz\u00e1lez-Miguel, J. (2023). Fasciola hepatica juveniles interact with the host fibrinolytic system as a potential early-stage invasion mechanism. PLoS Negl. Trop. Dis., 17.","DOI":"10.1371\/journal.pntd.0010936"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1523\/JNEUROSCI.20-05-02003.2000","article-title":"Laminin degradation by plasmin regulates long-term potentiation","volume":"20","author":"Nakagami","year":"2000","journal-title":"J. Neurosci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"101","DOI":"10.2174\/157016106776359880","article-title":"Plasminogen activator system and vascular disease","volume":"4","author":"Nicholl","year":"2006","journal-title":"Curr. Vasc. Pharmacol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Becerro-Recio, D., Serrat, J., L\u00f3pez-Garc\u00eda, M., Sotillo, J., Sim\u00f3n, F., Gonz\u00e1lez-Miguel, J., and Siles-Lucas, M. (2022). Proteomics coupled with in vitro model to study the early crosstalk occurring between newly excysted juveniles of Fasciola hepatica and host intestinal cells. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010811"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1891","DOI":"10.1074\/mcp.M900045-MCP200","article-title":"An integrated transcriptomics and proteomics analysis of the secretome of the helminth pathogen Fasciola hepatica","volume":"8","author":"Robinson","year":"2009","journal-title":"Mol. Cell. Proteom."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Becerro-Recio, D., Serrat, J., L\u00f3pez-Garc\u00eda, M., Molina-Hern\u00e1ndez, V., P\u00e9rez-Ar\u00e9valo, J., Mart\u00ednez-Moreno, \u00c1., Sotillo, J., Sim\u00f3n, F., Gonz\u00e1lez-Miguel, J., and Siles-Lucas, M. (2022). Study of the migration of Fasciola hepatica juveniles across the intestinal barrier of the host by quantitative proteomics in an ex vivo model. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010766"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1016\/j.tcb.2020.06.004","article-title":"Cytoskeletal crosstalk in cell migration","volume":"30","author":"Seetharaman","year":"2020","journal-title":"Trends Cell. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1002\/(SICI)1097-0177(200006)218:2<213::AID-DVDY1>3.0.CO;2-R","article-title":"Form and function: The laminin family of heterotrimers","volume":"218","author":"Colognato","year":"2000","journal-title":"Dev. Dyn."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1007\/s00109-007-0182-5","article-title":"Laminin isoforms in development and disease","volume":"85","author":"Durbeej","year":"2007","journal-title":"J. Mol. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1182\/blood-2002-03-0796","article-title":"Laminin isoform\u2013specific promotion of adhesion and migration of human bone marrow progenitor cells","volume":"101","author":"Gu","year":"2003","journal-title":"Blood"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"McCusker, P., McVeigh, P., Rathinasamy, V., Toet, H., McCammick, E., O\u2019Connor, A., Marks, N.J., Mousley, A., Brennan, G.P., and Halton, D.W. (2016). Stimulating neoblast-like cell proliferation in juvenile Fasciola hepatica supports growth and progression towards the adult phenotype in vitro. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004994"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Cwiklinski, K., Robinson, M.W., Donnelly, S., and Dalton, J.P. (2021). Complementary transcriptomic and proteomic analyses reveal the cellular and molecular processes that drive growth and development of Fasciola hepatica in the host liver. BMC Genom., 22.","DOI":"10.1186\/s12864-020-07326-y"},{"key":"ref_26","first-page":"1491","article-title":"Laminin A5 modulates fibroblast proliferation in epidural fibrosis through the PI3K\/AKT\/MTOR signalling pathway","volume":"21","author":"Liu","year":"2020","journal-title":"Mol. Med. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Corvo, I., O\u2019Donoghue, A.J., Pastro, L., Pi-Denis, N., Eroy-Reveles, A., Roche, L., McKerrow, J.H., Dalton, J.P., Craik, C.S., and Caffrey, C.R. (2013). Dissecting the active Site of the collagenolytic cathepsin L3 protease of the invasive stage of Fasciola hepatica. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002269"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.molbiopara.2009.04.005","article-title":"The major cathepsin L secreted by the invasive juvenile Fasciola hepatica prefers proline in the S2 subsite and can cleave collagen","volume":"167","author":"Corvo","year":"2009","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Robinson, M.W., Corvo, I., Jones, P.M., George, A.M., Padula, M.P., To, J., Cancela, M., Rinaldi, G., Tort, J.F., and Roche, L. (2011). Collagenolytic activities of the major secreted cathepsin L peptidases involved in the virulence of the helminth pathogen, Fasciola hepatica. PLoS Negl. Trop. Dis., 5.","DOI":"10.1371\/journal.pntd.0001012"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1016\/j.biochi.2010.12.004","article-title":"Adult and juvenile Fasciola cathepsin L proteases: Different enzymes for different roles","volume":"93","author":"Norbury","year":"2011","journal-title":"Biochimie"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2307\/3284308","article-title":"Proteinases secreted by Fasciola hepatica degrade extracellular matrix and basement membrane components","volume":"83","author":"Berasain","year":"1997","journal-title":"J. Parasitol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1074\/jbc.275.2.817","article-title":"Self-assembly of laminin induced by acidic pH","volume":"275","author":"Freire","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Barbour, T., Cwiklinski, K., Lalor, R., Dalton, J.P., and de Marco Verissimo, C. (2021). The zoonotic helminth parasite Fasciola hepatica: Virulence-associated cathepsin B and cathepsin L cysteine peptidases secreted by infective newly excysted juveniles (NEJ). Animals, 11.","DOI":"10.3390\/ani11123495"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1055\/s-0033-1334483","article-title":"Plasminogen receptors: The first quarter century","volume":"39","author":"Miles","year":"2013","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"13778","DOI":"10.1074\/jbc.M111290200","article-title":"Exposure of cryptic domains in the alpha 1-chain of laminin-1 by elastase stimulates macrophages urokinase and matrix metalloproteinase-9 expression","volume":"277","author":"Laurie","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.actatropica.2013.06.023","article-title":"Parasitism in optima forma: Exploiting the host fibrinolytic system for invasion","volume":"128","author":"Figuera","year":"2013","journal-title":"Acta Tropica"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1051\/parasite\/2022034","article-title":"Interaction of helminth parasites with the haemostatic system of their vertebrate hosts: A scoping review","volume":"29","author":"Diosdado","year":"2022","journal-title":"Parasite"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.pt.2015.12.012","article-title":"Plasmin in parasitic chronic infections: Friend or foe?","volume":"32","author":"Kartashev","year":"2016","journal-title":"Trends Parasitol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1136\/gut.43.5.656","article-title":"Urokinase and the intestinal mucosa: Evidence for a role in epithelial cell turnover","volume":"43","author":"Gibson","year":"1998","journal-title":"Gut"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1134\/S1068162014060028","article-title":"Structure and function of plasminogen\/plasmin System","volume":"40","author":"Aisina","year":"2014","journal-title":"Russ. J. Bioorganic Chem."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Mattos, E.C., Canuto, G., Manchola, N.C., Magalh\u00e3es, R.D.M., Crozier, T.W.M., Lamont, D.J., Tavares, M.F.M., Colli, W., Ferguson, M.A.J., and Alves, M.J.M. (2019). Reprogramming of Trypanosoma cruzi metabolism triggered by parasite interaction with the host cell extracellular matrix. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007103"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0966-842X(96)10033-0","article-title":"Role of signalling and cytoskeletal rearrangements in the pathogenesis of Entamoeba histolytica","volume":"4","year":"1996","journal-title":"Trends Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Dalton, J.P. (2021). Fasciolosis, CABI. [2nd ed.].","DOI":"10.1079\/9781789246162.0000"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1002\/jimd.12248","article-title":"A biallelic pathogenic variant in the OGDH gene results in a neurological disorder with features of a mitochondrial disease","volume":"44","author":"Yap","year":"2021","journal-title":"J. Inherit. Metab. Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1007\/BF00714273","article-title":"2-ketoglutarate dehydrogenase deficiency, a rare cause of primary hyperlactataemia: Report of a new case","volume":"16","author":"Guffon","year":"1993","journal-title":"J. Inherit. Metab. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3383","DOI":"10.1074\/jbc.M109.060368","article-title":"Helminth cysteine proteases inhibit TRIF-dependent activation of macrophages via degradation of TLR3","volume":"285","author":"Donnelly","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/bs.apar.2019.01.001","article-title":"The cathepsin-like cysteine peptidases of trematodes of the genus Fasciola","volume":"Volume 104","author":"Cwiklinski","year":"2019","journal-title":"Advances in Parasitology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.3389\/fimmu.2020.02182","article-title":"Fasciola hepatica-derived molecules as regulators of the host immune response","volume":"11","author":"Ryan","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.molbiopara.2010.04.003","article-title":"Proteomic analysis of in vitro newly excysted juveniles from Fasciola hepatica","volume":"172","author":"Valero","year":"2010","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Garcia-Campos, A., Ravid\u00e0, A., Nguyen, D.L., Cwiklinski, K., Dalton, J.P., Hokke, C.H., O\u2019Neill, S., and Mulcahy, G. (2016). Tegument glycoproteins and cathepsins of newly excysted juvenile Fasciola hepatica carry mannosidic and paucimannosidic N-glycans. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004688"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"82","DOI":"10.3389\/fcimb.2015.00082","article-title":"Identification of novel laminin- and fibronectin-binding proteins by Far-Western Blot: Capturing the adhesins of Streptococcus suis type 2","volume":"5","author":"Li","year":"2015","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1111\/j.1567-1364.2012.00806.x","article-title":"Surface-expressed enolase contributes to the adhesion of Paracoccidioides brasiliensis to host cells","volume":"12","author":"Marcos","year":"2012","journal-title":"FEMS Yeast Res."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Miguel, J., Morch\u00f3n, R., Siles-Lucas, M., and Sim\u00f3n, F. (2015). Fibrinolysis and proliferative endarteritis: Two related processes in chronic infections? The model of the blood-borne pathogen Dirofilaria immitis. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0124445"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"De Marco Verissimo, C., Jewhurst, H.L., Dob\u00f3, J., G\u00e1l, P., Dalton, J.P., and Cwiklinski, K. (2022). Fasciola hepatica is refractory to complement killing by preventing attachment of mannose binding lectin (MBL) and inhibiting MBL-associated serine proteases (MASPs) with serpins. PLoS Pathog., 18.","DOI":"10.1371\/journal.ppat.1010226"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"14440","DOI":"10.1073\/pnas.93.25.14440","article-title":"Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gels","volume":"93","author":"Shevchenko","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1638","DOI":"10.1074\/mcp.T600050-MCP200","article-title":"The Paragon algorithm, a next generation search engine that uses sequence temperature values and feature probabilities to identify peptides from tandem mass spectra","volume":"6","author":"Shilov","year":"2007","journal-title":"Mol. Cell. Proteom."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Mattos, E.C., Schumacher, R.I., Colli, W., and Alves, M.J.M. (2012). Adhesion of Trypanosoma cruzi trypomastigotes to fibronectin or laminin modifies tubulin and paraflagellar rod protein phosphorylation. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0046767"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"16043","DOI":"10.1038\/mtm.2016.43","article-title":"Moving toward a higher efficiency of microcell-mediated chromosome transfer","volume":"3","author":"Liskovykh","year":"2016","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"ref_60","unstructured":"Fox, J., and Bouchet-Valat, M. (2022, March 10). Rcmdr: R Commander. R Package Version 2.8-0. Available online: https:\/\/socialsciences.mcmaster.ca\/jfox\/Misc\/Rcmdr\/."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/9\/8165\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:28:34Z","timestamp":1760124514000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/9\/8165"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,3]]},"references-count":60,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["ijms24098165"],"URL":"https:\/\/doi.org\/10.3390\/ijms24098165","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,3]]}}}