{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:55:51Z","timestamp":1790686551779,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T00:00:00Z","timestamp":1683244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical University in Lublin","award":["DS 120\/2018-2020"],"award-info":[{"award-number":["DS 120\/2018-2020"]}]},{"name":"Medical University in Lublin","award":["DS 640"],"award-info":[{"award-number":["DS 640"]}]},{"name":"Medical University in Lublin","award":["GI\/1"],"award-info":[{"award-number":["GI\/1"]}]},{"name":"Medical University in Lublin","award":["DS 29"],"award-info":[{"award-number":["DS 29"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>According to recent data, changes in the vaginal microbiota could affect the risk of gynaecological cancers. Women suffering from endometrial cancer present significant changes in cervicovaginal microbiota composition. The objective of our study was to characterize the cervicovaginal microbiota of women undergoing hysterectomy due to benign disease, atypical hyperplasia, and endometrial cancer; The study included 96 patients, who undergone surgical treatment due to benign uterine disease, precancerous endometrial lesion, and endometrial cancer. Quantitative and qualitative real-time PCR analysis of DNA isolated from vaginal fornix and endocervical canal samples was performed to detect the 19 most commonly identified microorganisms, including different Lactobacillus spp., Atopobium, Bifidobacterium, Chlamydia, and Gardnerella; At least one of the tested microorganisms was identified in 88.5% of vaginal and 83.3% of cervical samples. Lactobacillus iners was significantly more frequent in patients with benign condition, whereas Dialister pneumosintes and Mobiluncus curtisii was more frequent in cancer patients; Mobiluncus curtisi and Dialister pneumosintes, which were identified as significantly more common in endometrial cancer vaginal samples, may be considered as potential endometrial cancer co-factors which promote\/stimulate carcinogenesis. However, the exact mechanism of such activity remains unexplained and requires further investigations.<\/jats:p>","DOI":"10.3390\/ijms24098266","type":"journal-article","created":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T03:24:32Z","timestamp":1683257072000},"page":"8266","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Vaginal and Cervical Microbiota Composition in Patients with Endometrial Cancer"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0455-0371","authenticated-orcid":false,"given":"Bart\u0142omiej","family":"Barczy\u0144ski","sequence":"first","affiliation":[{"name":"1st Department of Oncological Gynaecology and Gynaecology, Medical University in Lublin, 20-081 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Fr\u0105szczak","sequence":"additional","affiliation":[{"name":"1st Department of Oncological Gynaecology and Gynaecology, Medical University in Lublin, 20-081 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0451-4741","authenticated-orcid":false,"given":"Ewelina","family":"Grywalska","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunology, Medical University in Lublin, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2300-3266","authenticated-orcid":false,"given":"Jan","family":"Kotarski","sequence":"additional","affiliation":[{"name":"Independent Laboratory of Cancer Diagnostics and Immunology, Medical University in Lublin, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1634-0387","authenticated-orcid":false,"given":"Izabela","family":"Korona-G\u0142owniak","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Medical University in Lublin, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/s41585-020-0286-z","article-title":"The microbiome and gynaecological cancer development, prevention and therapy","volume":"17","author":"Ilhan","year":"2020","journal-title":"Nat. Rev. Urol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1038\/nm.4517","article-title":"Current understanding of the human microbiome","volume":"24","author":"Gilbert","year":"2018","journal-title":"Nat. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.ygyno.2015.04.036","article-title":"The vaginal and gastrointestinal microbiomes in gynecologic cancers: A review of applications in etiology, symptoms and treatment","volume":"138","author":"Chase","year":"2015","journal-title":"Gynecol. Oncol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Hernandes, C., Silveira, P., Rodrigues Sereia, A.F., Christoff, A.P., Mendes, H., Valter de Oliveira, L.F., and Podgaec, S. (2020). Microbiome Profile of Deep Endometriosis Patients: Comparison of Vaginal Fluid, Endometrium and Lesion. Diagnostics, 10.","DOI":"10.3390\/diagnostics10030163"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.fertnstert.2019.05.015","article-title":"Endometrial Microbiota in Infertile Women with and without Chronic Endometritis as Diagnosed Using a Quantitative and Reference Range-Based Method","volume":"112","author":"Liu","year":"2019","journal-title":"Fertil. Steril."},{"key":"ref_6","first-page":"1581","article-title":"Barcoded sequencing reveals diverse intrauterine microbiomes in patients suffering with endometrial polyps","volume":"8","author":"Fang","year":"2016","journal-title":"Am. J. Transl. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1007\/s10482-017-0992-6","article-title":"A Role for the Endometrial Microbiome in Dysfunctional Menstrual Bleeding","volume":"111","author":"Pelzer","year":"2018","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"19213","DOI":"10.1038\/s41598-019-55720-8","article-title":"Postmenopause as a Key Factor in the Composition of the Endometrial Cancer Microbiome (ECbiome)","volume":"9","author":"Walsh","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1111\/ajo.12754","article-title":"A Retrospective Pilot Study to Determine Whether the Reproductive Tract Microbiota Differs between Women with a History of Infertility and Fertile Women","volume":"58","author":"Wee","year":"2018","journal-title":"Aust. N. Z. J. Obstet. Gynaecol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1097\/PPO.0000000000000048","article-title":"Microbiome, Inflammation, and Cancer","volume":"20","author":"Francescone","year":"2014","journal-title":"Cancer J."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Sobstyl, M., Brecht, P., Sobstyl, A., Mertowska, P., and Grywalska, E. (2022). The Role of Microbiota in the Immunopathogenesis of Endometrial Cancer. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23105756"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1097\/PPO.0000000000000049","article-title":"The microbiome and obesity-an established risk for certain types of cancer","volume":"20","author":"Rogers","year":"2014","journal-title":"Cancer J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"dmm035147","DOI":"10.1242\/dmm.035147","article-title":"The relationship between sex hormones, the vaginal microbiome and immunity in HIV-1 susceptibility in women","volume":"11","author":"Wessels","year":"2018","journal-title":"Dis. Model. Mech."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/j.1574-695X.2009.00618.x","article-title":"The vaginal bacterial communities of Japanese women resemble those of women in other racial groups","volume":"58","author":"Zhou","year":"2010","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"97","DOI":"10.3389\/fphys.2015.00097","article-title":"Individualized medicine and the microbiome in reproductive tract","volume":"6","author":"Braundmeier","year":"2015","journal-title":"Front Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4680","DOI":"10.1073\/pnas.1002611107","article-title":"Vaginal microbiome of reproductive-age women","volume":"108","author":"Ravel","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1186\/s13073-016-0368-y","article-title":"Potential contribution of the uterine microbiome in the development of endometrial cancer","volume":"8","author":"Chen","year":"2016","journal-title":"Genome Med."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Gressel, G.M., Usyk, M., Frimer, M., Kuo, D.Y.S., and Burk, R.D. (2021). Characterization of the endometrial, cervicovaginal and anorectal microbiota in post-menopausal women with endometrioid and serous endometrial cancers. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0259188"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1158\/2767-9764.CRC-22-0075","article-title":"The vaginal microbiome is associated with endometrial cancer grade and histology","volume":"2","author":"Hakimjavadi","year":"2022","journal-title":"Cancer Res. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"928475","DOI":"10.3389\/fimmu.2022.928475","article-title":"\u201cIron triangle\u201d of regulating the uterine microecology: Endometrial microbiota, immunity and endometrium","volume":"13","author":"Zhu","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Baj, J., Korona-G\u0142owniak, I., Forma, A., Maani, A., Sitarz, E., Rahnama-Hezavah, M., Radzikowska, E., and Portincasa, P. (2020). Mechanisms of the Epithelial-Mesenchymal Transition and Tumor Microenvironment in Helicobacter pylori-Induced Gastric Cancer. Cells, 23.","DOI":"10.3390\/cells9041055"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.tim.2022.08.010","article-title":"Fusobacterium nucleatum, a key pathogenic factor and microbial biomarker for colorectal cancer","volume":"31","author":"Wang","year":"2023","journal-title":"Trends Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s12672-019-0358-9","article-title":"Estrogen Signaling in Endometrial Cancer: A Key Oncogenic Pathway with Several Open Questions","volume":"10","author":"Rodriguez","year":"2019","journal-title":"Horm. Cancer"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.chom.2011.10.003","article-title":"Microbiome and malignancy","volume":"20","author":"Plottel","year":"2011","journal-title":"Cell Host Microbe"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1111\/j.1574-6941.2008.00520.x","article-title":"Distribution of beta-glucosidase and beta-glucuronidase activity and of beta-glucuronidase gene gus in human colonic bacteria","volume":"66","author":"Dabek","year":"2008","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4539","DOI":"10.1073\/pnas.1000066107","article-title":"A metagenomic \u03b2-glucuronidase uncovers a core adaptive function of the human intestinal microbiome","volume":"15","author":"Gloux","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"826994","DOI":"10.3389\/fmicb.2022.826994","article-title":"\u03b2-Glucuronidase Pattern Predicted From Gut Metagenomes Indicates Potentially Diversified Pharmacomicrobiomics","volume":"13","author":"Candeliere","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1059825","DOI":"10.3389\/fcimb.2022.1059825","article-title":"Gut and genital tract microbiomes: Dysbiosis and link to gynecological disorders","volume":"12","author":"Elkafas","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1677\/ERC-10-0053","article-title":"Obesity, inflammatory markers, and endometrial cancer risk: A prospective case-control study","volume":"17","author":"Dossus","year":"2010","journal-title":"Endocr. Relat. Cancer"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"501","DOI":"10.3748\/wjg.v22.i2.501","article-title":"Gut microbiota imbalance and colorectal cancer","volume":"22","author":"Raisch","year":"2016","journal-title":"World J. Gastroenterol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.1002\/ijc.33428","article-title":"Dysbiosis of the endometrial microbiota and its association with inflammatory cytokines in endometrial cancer","volume":"148","author":"Lu","year":"2021","journal-title":"Int. J. Cancer"},{"key":"ref_32","first-page":"56","article-title":"Characterization of Gardnerella vaginalis isolates: Correlations among clades, biofilm formation and cytokine stimulation","volume":"46","author":"Morselli","year":"2023","journal-title":"New Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/s40168-022-01317-9","article-title":"Gardnerella vaginalis alters cervicovaginal epithelial cell function through microbe-specific immune responses","volume":"10","author":"Anton","year":"2022","journal-title":"Microbiome"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"9747480","DOI":"10.1155\/2016\/9747480","article-title":"Slight Pro-Inflammatory Immunomodulation Properties of Dendritic Cells by Gardnerella vaginalis: The \u201cInvisible Man\u201d of Bacterial Vaginosis?","volume":"2016","author":"Bertran","year":"2016","journal-title":"J. Immunol. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1099\/mic.0.000608","article-title":"Anti-inflammatory effect of two Lactobacillus strains during infection with Gardnerella vaginalis and Candida albicans in a HeLa cell culture model","volume":"164","author":"Santos","year":"2018","journal-title":"Microbiology"},{"key":"ref_36","first-page":"88","article-title":"Bacterial vaginosis and health-associated bacteria modulate the immunometabolic landscape in 3D model of human cervix","volume":"13","year":"2021","journal-title":"NPJ Biofilms Microbiomes"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"11974","DOI":"10.1038\/s41598-017-12198-6","article-title":"A longitudinal analysis of the vaginal microbiota and vaginal immune mediators in women from sub-Saharan Africa","volume":"7","author":"Jespers","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Wu, Y., Wang, C.Z., Wan, J.Y., Yao, H., and Yuan, C.S. (2021). Dissecting the Interplay Mechanism between Epigenetics and Gut Microbiota: Health Maintenance and Disease Prevention. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22136933"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Inoue, F., Sone, K., Toyohara, Y., Takahashi, Y., Kukita, A., Hara, A., Taguchi, A., Tanikawa, M., Tsuruga, T., and Osuga, Y. (2021). Targeting Epigenetic Regulators for Endometrial Cancer Therapy: Its Molecular Biology and Potential Clinical Applications. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22052305"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"171","DOI":"10.23736\/S0026-4784.18.04322-8","article-title":"Epigenetics and the vaginal microbiome: Influence of the microbiota on the histone deacetylase level in vaginal epithelial cells from pregnant women","volume":"71","author":"Witkin","year":"2019","journal-title":"Minerva Ginecol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.3389\/fmicb.2018.02181","article-title":"Common Cervicovaginal Microbial Supernatants Alter Cervical Epithelial Function: Mechanisms by Which Lactobacillus crispatus Contributes to Cervical Health","volume":"9","author":"Anton","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.ygyno.2022.02.021","article-title":"Differences in the microbial profiles of early stage endometrial cancers between Black and White women","volume":"165","author":"Hawkins","year":"2022","journal-title":"Gynecol. Oncol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e11574","DOI":"10.7717\/peerj.11574","article-title":"Shifts in gut and vaginal microbiomes are associated with cancer recurrence time in women with ovarian cancer","volume":"9","author":"Jacobson","year":"2021","journal-title":"PeerJ."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4654","DOI":"10.1158\/0008-5472.CAN-22-0455","article-title":"Disruption of the Gut Microbiota Confers Cisplatin Resistance in Epithelial Ovarian Cancer","volume":"82","author":"Chambers","year":"2022","journal-title":"Cancer Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/9\/8266\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:29:29Z","timestamp":1760124569000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/9\/8266"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,5]]},"references-count":44,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["ijms24098266"],"URL":"https:\/\/doi.org\/10.3390\/ijms24098266","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,5]]}}}