{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T02:22:31Z","timestamp":1776046951681,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T00:00:00Z","timestamp":1683244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["R01 AI127401"],"award-info":[{"award-number":["R01 AI127401"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Proteus mirabilis is a Gram-negative Gammaproteobacterium and a major causative agent of urinary tract infections in humans. It is characterized by its ability to switch between swimming motility in liquid media and swarming on solid surfaces. Here, we used cryo-electron tomography and subtomogram averaging to reveal the structure of the flagellar motor of P. mirabilis at nanometer resolution in intact cells. We found that P. mirabilis has a motor that is structurally similar to those of Escherichia coli and Salmonella enterica, lacking the periplasmic elaborations that characterize other more specialized gammaproteobacterial motors. In addition, no density corresponding to stators was present in the subtomogram average suggesting that the stators are dynamic. Finally, several assembly intermediates of the motor were seen that support the inside-out assembly pathway.<\/jats:p>","DOI":"10.3390\/ijms24098292","type":"journal-article","created":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T09:18:09Z","timestamp":1683278289000},"page":"8292","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Structure and Assembly of the Proteus mirabilis Flagellar Motor by Cryo-Electron Tomography"],"prefix":"10.3390","volume":"24","author":[{"given":"Mohammed","family":"Kaplan","sequence":"first","affiliation":[{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Yao","sequence":"additional","affiliation":[{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1556-4864","authenticated-orcid":false,"given":"Grant J.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA"},{"name":"Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84604, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1099\/00221287-40-1-29","article-title":"Development of Flagella by Proteus mirabilis","volume":"40","author":"Hoeniger","year":"1965","journal-title":"J. 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