{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T14:28:31Z","timestamp":1774880911470,"version":"3.50.1"},"reference-count":51,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T00:00:00Z","timestamp":1683590400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["SQ2022YFD1300007"],"award-info":[{"award-number":["SQ2022YFD1300007"]}]},{"name":"National Key Research and Development Program of China","award":["32272889"],"award-info":[{"award-number":["32272889"]}]},{"name":"National Key Research and Development Program of China","award":["NCTIP XD\/B04"],"award-info":[{"award-number":["NCTIP XD\/B04"]}]},{"name":"National Natural Science Foundation of China","award":["SQ2022YFD1300007"],"award-info":[{"award-number":["SQ2022YFD1300007"]}]},{"name":"National Natural Science Foundation of China","award":["32272889"],"award-info":[{"award-number":["32272889"]}]},{"name":"National Natural Science Foundation of China","award":["NCTIP XD\/B04"],"award-info":[{"award-number":["NCTIP XD\/B04"]}]},{"name":"National Center of Technology Innovation for Pigs","award":["SQ2022YFD1300007"],"award-info":[{"award-number":["SQ2022YFD1300007"]}]},{"name":"National Center of Technology Innovation for Pigs","award":["32272889"],"award-info":[{"award-number":["32272889"]}]},{"name":"National Center of Technology Innovation for Pigs","award":["NCTIP XD\/B04"],"award-info":[{"award-number":["NCTIP XD\/B04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study was conducted to evaluate the effects of a low-protein (LP) diet supplemented with sodium butyrate (SB), medium-chain fatty acids (MCFAs) and n-3 polyunsaturated fatty acids (PUFAs) on nutrient utilization and lipid and amino acid metabolism in weaned pigs. A total of 120 Duroc \u00d7 Landrace \u00d7 Yorkshire pigs (initial body weight: 7.93 \u00b1 0.65 kg) were randomly assigned to five dietary treatments, including the control diet (CON), LP diet, LP + 0.2% SB diet (LP + SB), LP + 0.2% MCFA diet (LP + MCFA) and LP + 0.2% n-3 PUFA diet (LP + PUFA). The results show that the LP + MCFA diet increased (p &lt; 0.05) the digestibility of dry matter and total P in pigs compared with the CON and LP diets. In the liver of the pigs, the metabolites involved in sugar metabolism and oxidative phosphorylation significantly changed with the LP diet compared with the CON diet. Compared with the LP diet, the altered metabolites in the liver of the pigs fed with the LP + SB diet were mainly associated with sugar metabolism and pyrimidine metabolism; the altered metabolites in the liver of pigs fed with the LP + MCFA and LP + PUFA diets were mainly associated with lipid metabolism and amino acid metabolism. In addition, the LP + PUFA diet increased (p &lt; 0.05) the concentration of glutamate dehydrogenase in the liver of pigs compared with the LP diet. Furthermore, the LP + MCFA and LP + PUFA diets increased (p &lt; 0.05) the mRNA abundance of sterol regulatory element-binding protein 1 and acetyl-CoA carboxylase in the liver compared with the CON diet. The LP + PUFA diet increased (p &lt; 0.05) mRNA abundances of fatty acid synthase in the liver compared with the CON and LP diets. Collectively, the LP diet supplemented with MCFAs improved nutrient digestibility, and the LP diet supplemented with MCFAs and n-3 PUFAs promoted lipid and amino acid metabolisms.<\/jats:p>","DOI":"10.3390\/ijms24108501","type":"journal-article","created":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T03:32:27Z","timestamp":1683689547000},"page":"8501","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Different Fatty Acid Supplementation in Low-Protein Diets Regulate Nutrient Utilization and Lipid and Amino Acid Metabolism in Weaned Pigs Model"],"prefix":"10.3390","volume":"24","author":[{"given":"Qingsong","family":"Tang","sequence":"first","affiliation":[{"name":"Laboratory for Bio-Feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenxue","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory for Bio-Feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongxiang","family":"Ren","sequence":"additional","affiliation":[{"name":"Laboratory for Bio-Feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Ding","sequence":"additional","affiliation":[{"name":"Laboratory for Bio-Feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xie","family":"Peng","sequence":"additional","affiliation":[{"name":"Laboratory for Bio-Feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8465-4631","authenticated-orcid":false,"given":"Zhiru","family":"Tang","sequence":"additional","affiliation":[{"name":"Laboratory for Bio-Feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaman","family":"Pang","sequence":"additional","affiliation":[{"name":"Laboratory for Bio-Feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yetong","family":"Xu","sequence":"additional","affiliation":[{"name":"Laboratory for Bio-Feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihong","family":"Sun","sequence":"additional","affiliation":[{"name":"Laboratory for Bio-Feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s40104-018-0276-7","article-title":"Advances in low-protein diets for swine","volume":"9","author":"Wang","year":"2018","journal-title":"J. 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