{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:14:09Z","timestamp":1788455649716,"version":"build-2803163510"},"reference-count":39,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T00:00:00Z","timestamp":1684108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Grant-in-Aid for Scientific Research (A)","doi-asserted-by":"publisher","award":["19H00965"],"award-info":[{"award-number":["19H00965"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Grant-in-Aid for Scientific Research (A)","doi-asserted-by":"publisher","award":["23H00354"],"award-info":[{"award-number":["23H00354"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Grant-in-Aid for Scientific Research (A)","doi-asserted-by":"publisher","award":["JPJ007097"],"award-info":[{"award-number":["JPJ007097"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Grant-in-Aid for Scientific Research (A)","doi-asserted-by":"publisher","award":["JP21zf0127001"],"award-info":[{"award-number":["JP21zf0127001"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Grant-in-Aid for Scientific Research (A)","doi-asserted-by":"publisher","award":["JPMJFS2102"],"award-info":[{"award-number":["JPMJFS2102"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Project of the Bio-Oriented Technology Research Advancement Institution","award":["19H00965"],"award-info":[{"award-number":["19H00965"]}]},{"name":"Project of the Bio-Oriented Technology Research Advancement Institution","award":["23H00354"],"award-info":[{"award-number":["23H00354"]}]},{"name":"Project of the Bio-Oriented Technology Research Advancement Institution","award":["JPJ007097"],"award-info":[{"award-number":["JPJ007097"]}]},{"name":"Project of the Bio-Oriented Technology Research Advancement Institution","award":["JP21zf0127001"],"award-info":[{"award-number":["JP21zf0127001"]}]},{"name":"Project of the Bio-Oriented Technology Research Advancement Institution","award":["JPMJFS2102"],"award-info":[{"award-number":["JPMJFS2102"]}]},{"name":"Frontier Research in Duo","award":["19H00965"],"award-info":[{"award-number":["19H00965"]}]},{"name":"Frontier Research in Duo","award":["23H00354"],"award-info":[{"award-number":["23H00354"]}]},{"name":"Frontier Research in Duo","award":["JPJ007097"],"award-info":[{"award-number":["JPJ007097"]}]},{"name":"Frontier Research in Duo","award":["JP21zf0127001"],"award-info":[{"award-number":["JP21zf0127001"]}]},{"name":"Frontier Research in Duo","award":["JPMJFS2102"],"award-info":[{"award-number":["JPMJFS2102"]}]},{"name":"Japan Science and Technology Agency","award":["19H00965"],"award-info":[{"award-number":["19H00965"]}]},{"name":"Japan Science and Technology Agency","award":["23H00354"],"award-info":[{"award-number":["23H00354"]}]},{"name":"Japan Science and Technology Agency","award":["JPJ007097"],"award-info":[{"award-number":["JPJ007097"]}]},{"name":"Japan Science and Technology Agency","award":["JP21zf0127001"],"award-info":[{"award-number":["JP21zf0127001"]}]},{"name":"Japan Science and Technology Agency","award":["JPMJFS2102"],"award-info":[{"award-number":["JPMJFS2102"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Klebsiella pneumoniae is an opportunistic pathogen that can produce moderate and severe infections in immunosuppressed hosts. In recent years, an increase in the isolation of hypermucoviscous carbapenem-resistant K. pneumoniae with sequence type 25 (ST25) in hospitals in Norwest Argentina was observed. This work aimed to study the virulence and inflammatory potential of two K. pneumoniae ST25 strains (LABACER01 and LABACER27) in the intestinal mucosa. The human intestinal Caco-2 cells were infected with the K. pneumoniae ST25 strains, and their adhesion and invasion rates and changes in the expression of tight junction and inflammatory factors genes were evaluated. ST25 strains were able to adhere and invade Caco-2 cells, reducing their viability. Furthermore, both strains reduced the expression of tight junction proteins (occludin, ZO-1, and claudin-5), altered permeability, and increased the expression of TGF-\u03b2 and TLL1 and the inflammatory factors (COX-2, iNOS, MCP-1, IL-6, IL-8, and TNF-\u03b1) in Caco-2 cells. The inflammatory response induced by LABACER01 and LABACER27 was significantly lower than the one produced by LPS or other intestinal pathogens, including K. pneumoniae NTUH-K2044. No differences in virulence and inflammatory potential were found between LABACER01 and LABACER27. In line with these findings, no major differences between the strains were found when the comparative genomic analysis of virulence factors associated with intestinal infection\/colonization was performed. This work is the first to demonstrate that hypermucoviscous carbapenem-resistant K. pneumoniae ST25 infects human intestinal epithelial cells and induces moderate inflammation.<\/jats:p>","DOI":"10.3390\/ijms24108804","type":"journal-article","created":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T02:37:51Z","timestamp":1684204671000},"page":"8804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1371-0688","authenticated-orcid":false,"given":"Stefania","family":"Dentice Maidana","sequence":"first","affiliation":[{"name":"Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina"},{"name":"Laboratory of Antimicrobials, Institute of Microbiology \u201cLuis C. Verna\u201d, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucuman, Tucuman 4000, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano","family":"Elean","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohtaro","family":"Fukuyama","sequence":"additional","affiliation":[{"name":"Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan"},{"name":"Livestock Immunology Unit, International Education and Research Centre for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiya","family":"Imamura","sequence":"additional","affiliation":[{"name":"Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan"},{"name":"Livestock Immunology Unit, International Education and Research Centre for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Albarrac\u00edn","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudeb","family":"Saha","sequence":"additional","affiliation":[{"name":"Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan"},{"name":"Livestock Immunology Unit, International Education and Research Centre for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan"},{"name":"Department of Dairy Science, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet 3100, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihito","family":"Suda","sequence":"additional","affiliation":[{"name":"Department of Food, Agriculture and Environment, Miyagi University, Sendai 980-8572, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoichiro","family":"Kurata","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda \u00c1ngela","family":"Jure","sequence":"additional","affiliation":[{"name":"Laboratory of Antimicrobials, Institute of Microbiology \u201cLuis C. Verna\u201d, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucuman, Tucuman 4000, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4586-5553","authenticated-orcid":false,"given":"Haruki","family":"Kitazawa","sequence":"additional","affiliation":[{"name":"Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan"},{"name":"Livestock Immunology Unit, International Education and Research Centre for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4941-4960","authenticated-orcid":false,"given":"Julio","family":"Villena","sequence":"additional","affiliation":[{"name":"Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina"},{"name":"Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8577, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.30802\/AALAS-CM-19-000067","article-title":"Clinical and pathologic features of spontaneous Klebsiella pneumoniae infection in 9 rhesus macaques (Macaca mulatta)","volume":"70","author":"Keesler","year":"2020","journal-title":"Comp. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1128\/MMBR.00078-15","article-title":"Klebsiella pneumoniae: Going on the offense with a strong defense","volume":"80","author":"Paczosa","year":"2016","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"64","DOI":"10.12703\/P6-64","article-title":"Klebsiella: A long way to go towards understanding this enigmatic jet-setter","volume":"6","author":"Broberg","year":"2014","journal-title":"F1000Prime Rep."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Lin, Y.T., Siu, L.K., Lin, J.C., Chen, T.L., Tseng, C.P., Yeh, K.M., Chang, F.Y., and Fung, C.P. (2012). Seroepidemiology of Klebsiella pneumoniae colonizing the intestinal tract of healthy Chinese and overseas Chinese adults in Asian countries. BMC Microbiol., 12.","DOI":"10.1186\/1471-2180-12-13"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e00001-19","DOI":"10.1128\/CMR.00001-19","article-title":"Hypervirulent Klebsiella pneumoniae","volume":"32","author":"Russo","year":"2019","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1016\/S1473-3099(22)00275-4","article-title":"Global evolution and geographic diversity of hypervirulent carbapenem-resistant Klebsiella pneumoniae","volume":"22","author":"Wu","year":"2022","journal-title":"Lancet Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e00071-20","DOI":"10.1128\/IAI.00071-20","article-title":"Animal model to study Klebsiella pneumoniae gastrointestinal colonization and host-to-host transmission","volume":"88","author":"Young","year":"2020","journal-title":"Infect. Immun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"151561","DOI":"10.1016\/j.ijmm.2022.151561","article-title":"Klebsiella pneumoniae activates the TGF-beta signaling pathway to adhere to and invade intestinal epithelial cells via enhancing TLL1 expression","volume":"312","author":"Liu","year":"2022","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1099\/jmm.0.46165-0","article-title":"Investigation of the putative virulence gene magA in a worldwide collection of 495 Klebsiella isolates: magA is restricted to the gene cluster of Klebsiella pneumoniae capsule serotype K1","volume":"54","author":"Struve","year":"2005","journal-title":"J. Med. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.3389\/fmicb.2015.01435","article-title":"Rapid Detection of K1 Hypervirulent Klebsiella pneumoniae by MALDI-TOF MS","volume":"6","author":"Huang","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"642484","DOI":"10.3389\/fmicb.2021.642484","article-title":"Virulence factors in hypervirulent Klebsiella pneumoniae","volume":"12","author":"Zhu","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1128\/JB.185.3.788-800.2003","article-title":"RmpA2, an activator of capsule biosynthesis in Klebsiella pneumoniae CG43, regulates K2 cps gene expression at the transcriptional level","volume":"185","author":"Lai","year":"2003","journal-title":"J. Bacteriol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3144","DOI":"10.1128\/JB.00031-10","article-title":"RmpA regulation of capsular polysaccharide biosynthesis in Klebsiella pneumoniae CG43","volume":"192","author":"Cheng","year":"2010","journal-title":"J. Bacteriol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e02663-18","DOI":"10.1128\/mBio.02663-18","article-title":"Genome-wide screening for enteric colonization factors in carbapenem-resistant ST258 Klebsiella pneumoniae","volume":"10","author":"Jung","year":"2019","journal-title":"mBio"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1093\/infdis\/jiy534","article-title":"Klebsiella pneumoniae type VI secretion system contributes to bacterial competition, cell invasion, type-1 fimbriae expression, and in vivo colonization","volume":"219","author":"Hsieh","year":"2019","journal-title":"J. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1128\/IAI.02345-14","article-title":"Klebsiella pneumoniae translocates across the intestinal epithelium via Rho GTPase- and phosphatidylinositol 3-kinase\/Akt-dependent cell invasion","volume":"83","author":"Hsu","year":"2015","journal-title":"Infect. Immun."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e01829","DOI":"10.1016\/j.heliyon.2019.e01829","article-title":"Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae","volume":"5","author":"Vargas","year":"2019","journal-title":"Heliyon"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Dentice Maidana, S., Ortiz Moyano, R., Vargas, J.M., Fukuyama, K., Kurata, S., Melnikov, V., Jure, M.A., Kitazawa, H., and Villena, J. (2022). Respiratory Commensal bacteria increase protection against hypermucoviscous carbapenem-resistant Klebsiella pneumoniae ST25 infection. Pathogens, 11.","DOI":"10.3390\/pathogens11091063"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Albarracin, L., Ortiz Moyano, R., Vargas, J.M., Andrade, B.G.N., Cortez Zamar, J., Dentice Maidana, S., Fukuyama, K., Kurata, S., Jure, M.A., and Kitazawa, H. (2022). Genomic and immunological characterization of hypermucoviscous carbapenem-resistant Klebsiella pneumoniae ST25 isolates from Northwest Argentina. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23137361"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.jgar.2021.05.018","article-title":"Draft genome sequences of two hypermucoviscous carbapenem-resistant ST25 Klebsiella pneumoniae strains causing respiratory and systemic infections","volume":"26","author":"Jure","year":"2021","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"11537","DOI":"10.1073\/pnas.1001261107","article-title":"Escherichia coli induces DNA damage in vivo and triggers genomic instability in mammalian cells","volume":"107","author":"Petit","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.3390\/biom5031762","article-title":"Bacterial Genotoxins: Merging the DNA damage response into infection biology","volume":"5","author":"Grasso","year":"2015","journal-title":"Biomolecules"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"103","DOI":"10.3389\/fcimb.2017.00103","article-title":"Colibactin Contributes to the hypervirulence of pks(+) K1 CC23 Klebsiella pneumoniae in mouse meningitis infections","volume":"7","author":"Lu","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3789","DOI":"10.1038\/s41598-022-07827-8","article-title":"An animal model of limitation of gut colonization by carbapenemase-producing Klebsiella pneumoniae using rifaximin","volume":"12","author":"Xenofontos","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1126\/science.aan4526","article-title":"Ectopic colonization of oral bacteria in the intestine drives T(H)1 cell induction and inflammation","volume":"358","author":"Atarashi","year":"2017","journal-title":"Science"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3380","DOI":"10.1038\/s41598-019-39887-8","article-title":"Microbial colonization coordinates the pathogenesis of a Klebsiella pneumoniae infant isolate","volume":"9","author":"Pope","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Caballero, S., Carter, R., Ke, X., Susac, B., Leiner, I.M., Kim, G.J., Miller, L., Ling, L., Manova, K., and Pamer, E.G. (2015). Distinct but spatially overlapping intestinal niches for vancomycin-resistant Enterococcus faecium and carbapenem-resistant Klebsiella pneumoniae. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1005132"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"684","DOI":"10.3109\/00365521.2011.560678","article-title":"Klebsiella pneumoniae increases the risk of inflammation and colitis in a murine model of intestinal bowel disease","volume":"46","author":"Lee","year":"2011","journal-title":"Scand. J. Gastroenterol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Shi, Y., Zhao, W., Liu, G., Ali, T., Chen, P., Liu, Y., Kastelic, J.P., Han, B., and Gao, J. (2021). Bacteriophages isolated from dairy farm mitigated Klebsiella pneumoniae-induced inflammation in bovine mammary epithelial cells cultured in vitro. BMC Vet. Res., 17.","DOI":"10.1186\/s12917-020-02738-0"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1128\/IAI.00843-13","article-title":"Klebsiella pneumoniae induces an inflammatory response in an in vitro model of blood-retinal barrier","volume":"82","author":"Motta","year":"2014","journal-title":"Infect. Immun."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Fodah, R.A., Scott, J.B., Tam, H.H., Yan, P., Pfeffer, T.L., Bundschuh, R., and Warawa, J.M. (2014). Correlation of Klebsiella pneumoniae comparative genetic analyses with virulence profiles in a murine respiratory disease model. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0107394"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4596","DOI":"10.1128\/IAI.02212-14","article-title":"Early innate immunity to bacterial infection in the lung is regulated systemically by the commensal microbiota via nod-like receptor ligands","volume":"82","author":"Clarke","year":"2014","journal-title":"Infect. Immun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3446","DOI":"10.1099\/mic.0.050336-0","article-title":"The role of Klebsiella pneumoniae rmpA in capsular polysaccharide synthesis and virulence revisited","volume":"157","author":"Hsu","year":"2011","journal-title":"Microbiology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4492","DOI":"10.1128\/JB.00315-09","article-title":"Genome sequencing and comparative analysis of Klebsiella pneumoniae NTUH-K2044, a strain causing liver abscess and meningitis","volume":"191","author":"Wu","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1084\/jem.20030857","article-title":"A novel virulence gene in Klebsiella pneumoniae strains causing primary liver abscess and septic metastatic complications","volume":"199","author":"Fang","year":"2004","journal-title":"J. Exp. Med."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Storey, D., McNally, A., Astrand, M., Sa-Pessoa Graca Santos, J., Rodriguez-Escudero, I., Elmore, B., Palacios, L., Marshall, H., Hobley, L., and Molina, M. (2020). Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent. PLoS Pathog., 16.","DOI":"10.1371\/journal.ppat.1007969"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"16968","DOI":"10.1038\/s41598-022-21396-w","article-title":"Role of Klebsiella pneumoniae Type VI secretion system (T6SS) in long-term gastrointestinal colonization","volume":"12","author":"Merciecca","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Aziz, R.K., Bartels, D., Best, A.A., DeJongh, M., Disz, T., Edwards, R.A., Formsma, K., Gerdes, S., Glass, E.M., and Kubal, M. (2008). The RAST Server: Rapid annotations using subsystems technology. BMC Genom., 9.","DOI":"10.1186\/1471-2164-9-75"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1093\/bioinformatics\/btr039","article-title":"Easyfig: A genome comparison visualizer","volume":"27","author":"Sullivan","year":"2011","journal-title":"Bioinformatics"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/10\/8804\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:35:36Z","timestamp":1760124936000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/10\/8804"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,15]]},"references-count":39,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["ijms24108804"],"URL":"https:\/\/doi.org\/10.3390\/ijms24108804","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,15]]}}}