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Pigs","award":["2021YFYZ0007"],"award-info":[{"award-number":["2021YFYZ0007"]}]},{"name":"National Center of Technology Innovation for Pigs","award":["2021YFYZ0030"],"award-info":[{"award-number":["2021YFYZ0030"]}]},{"name":"National Center of Technology Innovation for Pigs","award":["scsztd-2023-08-09"],"award-info":[{"award-number":["scsztd-2023-08-09"]}]},{"name":"National Center of Technology Innovation for Pigs","award":["CARS-pig-35"],"award-info":[{"award-number":["CARS-pig-35"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Spermatogenesis is temperature-dependent, and the increase in testicular temperature seriously affects mammalian spermatogenesis and semen quality. In this study, the testicular heat stress model of mice was made with a 43 \u00b0C water bath for 25 min, and the effects of heat stress on semen quality and spermatogenesis-related regulators were analyzed. On the 7th day after heat stress, testis weight shrank to 68.45% and sperm density dropped to 33.20%. High-throughput sequencing analysis showed that 98 microRNAs (miRNAs) and 369 mRNAs were down-regulated, while 77 miRNAs and 1424 mRNAs were up-regulated after heat stress. Through gene ontology (GO) analysis of differentially expressed genes and miRNA\u2013mRNA co-expression networks, it was found that heat stress may be involved in the regulation of testicular atrophy and spermatogenesis disorders by affecting cell meiosis process and cell cycle. In addition, through functional enrichment analysis, co-expression regulatory network, correlation analysis and in vitro experiment, it was found that miR-143-3p may be a representative potential key regulatory factor affecting spermatogenesis under heat stress. In summary, our results enrich the understanding of miRNAs in testicular heat stress and provide a reference for the prevention and treatment of heat-stress-induced spermatogenesis disorders.<\/jats:p>","DOI":"10.3390\/ijms24108809","type":"journal-article","created":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T02:37:51Z","timestamp":1684204671000},"page":"8809","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9900-3559","authenticated-orcid":false,"given":"Mailin","family":"Gan","sequence":"first","affiliation":[{"name":"Key Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Farm Animal Genetic Resource Exploration and Innovation 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuewei","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, 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