{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:10:49Z","timestamp":1787505049327,"version":"build-2736575974"},"reference-count":55,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T00:00:00Z","timestamp":1684281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sheikh Hamdan Foundation for Medical Research, UAEU, Zayed Center for Health Sciences"},{"name":"College of Medicine and Health Sciences"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Silver nanoparticles are widely used in various industrial and biomedical applications; however, little is known about their potential cardiotoxicity after pulmonary exposure, particularly in hypertensive subjects. We assessed the cardiotoxicity of polyethylene glycol (PEG)-coated AgNPs in hypertensive (HT) mice. Saline (control) or PEG\u2013AgNPs (0.5 mg\/kg) were intratracheally (i.t.) instilled four times (on days 7, 14, 21, and 28 post-angiotensin II or vehicle [saline] infusion). On day 29, various cardiovascular parameters were evaluated. Systolic blood pressure and heart rate were higher in PEG\u2013AgNPs-treated HT mice than in saline-treated HT or PEG\u2013AgNPs-treated normotensive mice. The heart histology of PEG\u2013AgNPs-treated HT mice had comparatively larger cardiomyocyte damage with fibrosis and inflammatory cells when compared with saline-treated HT mice. Similarly, the relative heart weight and the activities of lactate dehydrogenase and creatine kinase-MB and the concentration of brain natriuretic peptide concentration were significantly augmented in heart homogenates of HT mice treated with PEG\u2013AgNPs compared with HT mice treated with saline or normotensive animals exposed to PEG\u2013AgNPs. Similarly, the concentrations of endothelin-1, P-selectin, vascular cell adhesion molecule-1, and intercellular adhesion molecule-1 in heart homogenates were significantly higher than in the other two groups when HT mice were exposed to PEG\u2013AgNPs. Markers of inflammation and oxidative and nitrosative stress were significantly elevated in heart homogenates of HT mice given PEG\u2013AgNPs compared with HT mice treated with saline or normotensive animals exposed to PEG\u2013AgNPs. The hearts of HT mice exposed to PEG\u2013AgNPs had significantly increased DNA damage than those of HT mice treated with saline or normotensive mice treated with AgNPs. In conclusion, the cardiac injury caused by PEG\u2013AgNPs was aggravated in hypertensive mice. The cardiotoxicity of PEG\u2013AgNPs in HT mice highlights the importance of an in-depth assessment of their toxicity before using them in clinical settings, particularly in patients with pre-existing cardiovascular diseases.<\/jats:p>","DOI":"10.3390\/ijms24108890","type":"journal-article","created":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T08:09:44Z","timestamp":1684397384000},"page":"8890","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Impact of Intratracheal Administration of Polyethylene Glycol-Coated Silver Nanoparticles on the Heart of Normotensive and Hypertensive Mice"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0699-1015","authenticated-orcid":false,"given":"Abderrahim","family":"Nemmar","sequence":"first","affiliation":[{"name":"Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain P.O. Box 17666, United Arab Emirates"},{"name":"Zayed Center for Health Sciences, United Arab Emirates University, Al Ain P.O. Box 17666, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhail","family":"Al-Salam","sequence":"additional","affiliation":[{"name":"Zayed Center for Health Sciences, United Arab Emirates University, Al Ain P.O. Box 17666, United Arab Emirates"},{"name":"Department of Pathology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain P.O Box 17666, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7147-6773","authenticated-orcid":false,"given":"Yaser E.","family":"Greish","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, United Arab Emirates University, Al Ain P.O. Box 17551, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumaya","family":"Beegam","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain P.O. Box 17666, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nur E.","family":"Zaaba","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain P.O. Box 17666, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6491-6278","authenticated-orcid":false,"given":"Badreldin H.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Clinical Pharmacy, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat 123, Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1007\/s00204-014-1447-8","article-title":"Role of silver nanoparticles (AgNPs) on the cardiovascular system","volume":"90","author":"Gonzalez","year":"2016","journal-title":"Arch. Toxicol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ferdous, Z., and Nemmar, A. (2020). Health Impact of Silver Nanoparticles: A Review of the Biodistribution and Toxicity Following Various Routes of Exposure. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21072375"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"279371","DOI":"10.1155\/2013\/279371","article-title":"Recent advances in particulate matter and nanoparticle toxicology: A review of the in vivo and in vitro studies","volume":"2013","author":"Nemmar","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.pharmthera.2013.06.008","article-title":"New perspectives in nanomedicine","volume":"140","author":"Thorley","year":"2013","journal-title":"Pharmacol. Ther."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"5564","DOI":"10.1021\/es200157h","article-title":"Aggregation kinetics of citrate and polyvinylpyrrolidone coated silver nanoparticles in monovalent and divalent electrolyte solutions","volume":"45","author":"Huynh","year":"2011","journal-title":"Environ. Sci. Technol."},{"key":"ref_6","first-page":"3603","article-title":"Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles","volume":"8","author":"England","year":"2013","journal-title":"Int. J. Nanomed."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.toxlet.2016.03.005","article-title":"The influence of Citrate or PEG coating on silver nanoparticle toxicity to a human keratinocyte cell line","volume":"249","author":"Bastos","year":"2016","journal-title":"Toxicol.Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2079630","DOI":"10.1155\/2022\/2079630","article-title":"Exacerbation of Thrombotic Responses to Silver Nanoparticles in Hypertensive Mouse Model","volume":"2022","author":"Ferdous","year":"2022","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.nano.2017.11.013","article-title":"Evaluation of cardiovascular responses to silver nanoparticles (AgNPs) in spontaneously hypertensive rats","volume":"14","author":"Gonzalez","year":"2018","journal-title":"Nanomedicine"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.ijpharm.2005.10.010","article-title":"Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles","volume":"307","author":"Owens","year":"2006","journal-title":"Int. J. Pharm."},{"key":"ref_11","first-page":"81","article-title":"The biological effects and possible modes of action of nanosilver","volume":"223","author":"Volker","year":"2013","journal-title":"Rev. Environ. Contam Toxicol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"879","DOI":"10.2217\/nnm.11.78","article-title":"Toxicological studies on silver nanoparticles: Challenges and opportunities in assessment, monitoring and imaging","volume":"6","author":"Stensberg","year":"2011","journal-title":"Nanomedicine"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1093\/toxsci\/55.1.24","article-title":"Intratracheal instillation as an exposure technique for the evaluation of respiratory tract toxicity: Uses and limitations","volume":"55","author":"Driscoll","year":"2000","journal-title":"Toxicol. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1165\/rcmb.2009-0427OC","article-title":"Particulate Matter Induces IL-6 Translocation from the Lung to the Systemic Circulation","volume":"44","author":"Kido","year":"2011","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"23936","DOI":"10.3390\/ijms151223936","article-title":"Inhalation of silver nanomaterials\u2014Seeing the risks","volume":"15","author":"Theodorou","year":"2014","journal-title":"Int. J. Mol. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"667","DOI":"10.3109\/17435390.2011.600840","article-title":"A health surveillance case study on workers who manufacture silver nanomaterials","volume":"6","author":"Lee","year":"2012","journal-title":"Nanotoxicology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3771","DOI":"10.1016\/j.biomaterials.2009.03.044","article-title":"The antimicrobial efficacy of sustained release silver-carbene complex-loaded L-tyrosine polyphosphate nanoparticles: Characterization, in vitro and in vivo studies","volume":"30","author":"Hindi","year":"2009","journal-title":"Biomaterials"},{"key":"ref_18","first-page":"1329","article-title":"Silver nanoparticles modify VEGF signaling pathway and mucus hypersecretion in allergic airway inflammation","volume":"7","author":"Jang","year":"2012","journal-title":"Int. J. Nanomed."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1093\/toxsci\/kfu265","article-title":"Pulmonary effects of silver nanoparticle size, coating, and dose over time upon intratracheal instillation","volume":"144","author":"Silva","year":"2015","journal-title":"Toxicol. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.taap.2019.01.006","article-title":"Pulmonary exposure to silver nanoparticles impairs cardiovascular homeostasis: Effects of coating, dose and time","volume":"367","author":"Ferdous","year":"2019","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"9639035","DOI":"10.1155\/2017\/9639035","article-title":"Cerium Oxide Nanoparticles in Lung Acutely Induce Oxidative Stress, Inflammation, and DNA Damage in Various Organs of Mice","volume":"2017","author":"Nemmar","year":"2017","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"H1540","DOI":"10.1152\/ajpheart.01270.2008","article-title":"Air pollution and cardiac remodeling: A role for RhoA\/Rho-kinase","volume":"296","author":"Ying","year":"2009","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s12989-015-0092-6","article-title":"Exposure to concentrated ambient particulate matter induces reversible increase of heart weight in spontaneously hypertensive rats","volume":"12","author":"Ying","year":"2015","journal-title":"Part. Fibre Toxicol."},{"key":"ref_24","first-page":"731","article-title":"Cardioprotective effects of Commiphora mukul against isoprenaline-induced cardiotoxicity: A biochemical and histopathological evaluation","volume":"32","author":"Ojha","year":"2011","journal-title":"J. Environ. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"R252","DOI":"10.1152\/ajpregu.00697.2007","article-title":"Exposure to inhaled particulate matter impairs cardiac function in senescent mice","volume":"295","author":"Tankersley","year":"2008","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0014-5793(93)80476-B","article-title":"Increased production of endothelin-1 in the hypertrophied rat heart due to pressure overload","volume":"332","author":"Yorikane","year":"1993","journal-title":"FEBS Lett."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.cellsig.2017.04.010","article-title":"Endothelin-1 promotes hypertrophic remodelling of cardiac myocytes by activating sustained signalling and transcription downstream of endothelin type A receptors","volume":"36","author":"Archer","year":"2017","journal-title":"Cell. Signal."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1161\/01.CIR.0000105701.98663.D4","article-title":"Conditional cardiac overexpression of endothelin-1 induces inflammation and dilated cardiomyopathy in mice","volume":"109","author":"Yang","year":"2004","journal-title":"Circulation"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"415","DOI":"10.2147\/VHRM.S64460","article-title":"A review about biomarkers for the investigation of vascular function and impairment in diabetes mellitus","volume":"12","author":"Derosa","year":"2016","journal-title":"Vasc. Health Risk Manag."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1161\/01.RES.82.5.576","article-title":"Expression and Regulation of Adhesion Molecules in Cardiac Cells by Cytokines","volume":"82","author":"Kacimi","year":"1998","journal-title":"Circ. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1161\/01.HYP.37.1.58","article-title":"Upregulated Expression of Rat Heart Intercellular Adhesion Molecule-1 in Angiotensin II\u2014but Not Phenylephrine\u2014Induced Hypertension","volume":"37","author":"Kiarash","year":"2001","journal-title":"Hypertension"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2923","DOI":"10.1113\/JP276758","article-title":"Endothelial cell-cardiomyocyte crosstalk in heart development and disease","volume":"598","author":"Colliva","year":"2020","journal-title":"J. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1146\/annurev.physiol.68.040104.124629","article-title":"Endothelial-cardiomyocyte interactions in cardiac development and repair","volume":"68","author":"Hsieh","year":"2006","journal-title":"Annu. Rev. Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Haryono, A., Ramadhiani, R., Ryanto, G.R., and Emoto, N. (2022). Endothelin and the Cardiovascular System: The Long Journey and Where We Are Going. Biology, 11.","DOI":"10.3390\/biology11050759"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.pharmthera.2016.05.006","article-title":"The heart as an extravascular target of endothelin-1 in particulate matter-induced cardiac dysfunction","volume":"165","author":"Chan","year":"2016","journal-title":"Pharmacol. Ther."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2118","DOI":"10.1161\/01.CIR.99.16.2118","article-title":"Expression of endothelin-1, endothelin-converting enzyme, and endothelin receptors in chronic heart failure","volume":"99","author":"Zolk","year":"1999","journal-title":"Circulation"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1164\/ajrccm.164.9.2101036","article-title":"Passage of intratracheally instilled ultrafine particles from the lung into the systemic circulation in hamster","volume":"164","author":"Nemmar","year":"2001","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_38","first-page":"827","article-title":"The acute toxic effects of silver nanoparticles on myocardial transmembrane potential, INa and IK1 channels and heart rhythm in mice","volume":"11","author":"Lin","year":"2017","journal-title":"Nanotoxicology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"6657","DOI":"10.1016\/j.biomaterials.2014.04.093","article-title":"Endothelial cell injury and dysfunction induced by silver nanoparticles through oxidative stress via IKK\/NF-kappaB pathways","volume":"35","author":"Shi","year":"2014","journal-title":"Biomaterials"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s12989-016-0159-z","article-title":"Impact of pulmonary exposure to gold core silver nanoparticles of different size and capping agents on cardiovascular injury","volume":"13","author":"Holland","year":"2016","journal-title":"Part. Fibre Toxicol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1177\/2047487319870344","article-title":"Oxidative stress and inflammation in the evolution of heart failure: From pathophysiology to therapeutic strategies","volume":"27","author":"Aimo","year":"2020","journal-title":"Eur. J. Prev. Cardiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1089\/ars.2013.5258","article-title":"The cooperative roles of inflammation and oxidative stress in the pathogenesis of hypertension","volume":"20","author":"Crowley","year":"2014","journal-title":"Antioxid. Redox Signal."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.mrrev.2014.09.001","article-title":"Oxidative stress and inflammation generated DNA damage by exposure to air pollution particles","volume":"762","author":"Moller","year":"2014","journal-title":"Mutat. Res. Rev. Mutat. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s12989-016-0124-x","article-title":"Tissue distribution and acute toxicity of silver after single intravenous administration in mice: Nano-specific and size-dependent effects","volume":"13","author":"Recordati","year":"2016","journal-title":"Part. Fibre Toxicol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1159\/000493460","article-title":"The in Vitro Effect of Polyvinylpyrrolidone and Citrate Coated Silver Nanoparticles on Erythrocytic Oxidative Damage and Eryptosis","volume":"49","author":"Ferdous","year":"2018","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1161\/01.CIR.103.3.448","article-title":"Angiotensin II-induced hypertension accelerates the development of atherosclerosis in apoE-deficient mice","volume":"103","author":"Weiss","year":"2001","journal-title":"Circulation"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.tox.2011.03.018","article-title":"Exacerbation of thrombotic events by diesel exhaust particle in mouse model of hypertension","volume":"285","author":"Nemmar","year":"2011","journal-title":"Toxicology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1056\/NEJMoa013591","article-title":"Endothelial function and oxidative stress in renovascular hypertension","volume":"346","author":"Higashi","year":"2002","journal-title":"N. Engl. J. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"H329","DOI":"10.1152\/ajpheart.00450.2016","article-title":"Chronic exposure to water-pipe smoke induces cardiovascular dysfunction in mice","volume":"312","author":"Nemmar","year":"2017","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1016\/j.phymed.2011.03.005","article-title":"Effect of Acacia gum on blood pressure in rats with adenine-induced chronic renal failure","volume":"18","author":"Ali","year":"2011","journal-title":"Phytomedicine"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"H917","DOI":"10.1152\/ajpheart.00313.2017","article-title":"Thrombosis, systemic and cardiac oxidative stress and DNA damage induced by pulmonary exposure to diesel exhaust particles, and the effect of nootkatone thereon","volume":"314","author":"Nemmar","year":"2018","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1159\/000443109","article-title":"Prolonged Pulmonary Exposure to Diesel Exhaust Particles Exacerbates Renal Oxidative Stress, Inflammation and DNA Damage in Mice with Adenine-Induced Chronic Renal Failure","volume":"38","author":"Nemmar","year":"2016","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Zaaba, N.E., Al-Salam, S., Beegam, S., Elzaki, O., Yasin, J., and Nemmar, A. (2023). Catalpol Attenuates Oxidative Stress and Inflammation via Mechanisms Involving Sirtuin-1 Activation and NF-\u03baB Inhibition in Experimentally-Induced Chronic Kidney Disease. Nutrients, 15.","DOI":"10.3390\/nu15010237"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Al Za\u2019abi, M., Ali, B.H., Al Suleimani, Y., Adham, S.A., Ali, H., Manoj, P., Ashique, M., and Nemmar, A. (2021). The Effect of Metformin in Diabetic and Non-Diabetic Rats with Experimentally-Induced Chronic Kidney Disease. Biomolecules, 11.","DOI":"10.3390\/biom11060814"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"896","DOI":"10.3389\/fphys.2017.00896","article-title":"Lung Oxidative Stress, DNA Damage, Apoptosis, and Fibrosis in Adenine-Induced Chronic Kidney Disease in Mice","volume":"8","author":"Nemmar","year":"2017","journal-title":"Front. Physiol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/10\/8890\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:36:53Z","timestamp":1760125013000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/10\/8890"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,17]]},"references-count":55,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["ijms24108890"],"URL":"https:\/\/doi.org\/10.3390\/ijms24108890","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,17]]}}}