{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T17:37:57Z","timestamp":1781977077041,"version":"3.54.5"},"reference-count":67,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,21]],"date-time":"2023-05-21T00:00:00Z","timestamp":1684627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Operational Programme Integrated infrastructure funded by the European Regional Development Fund","award":["ITMS2014+: 313011W410"],"award-info":[{"award-number":["ITMS2014+: 313011W410"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Despite significant advances in biomedical research, osteochondral defects resulting from injury, an autoimmune condition, cancer, or other pathological conditions still represent a significant medical problem. Even though there are several conservative and surgical treatment approaches, in many cases, they do not bring the expected results and further permanent damage to the cartilage and bones occurs. Recently, cell-based therapies and tissue engineering have gradually become promising alternatives. They combine the use of different types of cells and biomaterials to induce regeneration processes or replace damaged osteochondral tissue. One of the main challenges of this approach before clinical translation is the large-scale in vitro expansion of cells without changing their biological properties, while the use of conditioned media which contains various bioactive molecules appears to be very important. The presented manuscript provides a review of the experiments focused on osteochondral regeneration by using conditioned media. In particular, the effect on angiogenesis, tissue healing, paracrine signaling, and enhancing the properties of advanced materials are pointed out.<\/jats:p>","DOI":"10.3390\/ijms24109054","type":"journal-article","created":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T03:57:32Z","timestamp":1684727852000},"page":"9054","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Mesenchymal-Stromal-Cell-Conditioned Media and Their Implication for Osteochondral Regeneration"],"prefix":"10.3390","volume":"24","author":[{"given":"Dana","family":"Ivanisova","sequence":"first","affiliation":[{"name":"Regenmed Ltd., Medena 29, 811 01 Bratislava, Slovakia"},{"name":"Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Bohac","sequence":"additional","affiliation":[{"name":"Regenmed Ltd., Medena 29, 811 01 Bratislava, Slovakia"},{"name":"Centre for Tissue Engineering and Regenerative Medicine\u2013Translational Research Unit in the Branch of Regenerative Medicine, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Culenova","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovakia"},{"name":"National Institute of Rheumatic Diseases, N\u00e1bre\u017eie I. Krasku 4, 921 12 Pie\u0161\u0165any, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Smolinska","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovakia"},{"name":"National Institute of Rheumatic Diseases, N\u00e1bre\u017eie I. 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