{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T21:30:33Z","timestamp":1785879033806,"version":"3.56.0"},"reference-count":46,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education in Poland DS45 and DS6 projects of the Medical University of Lublin"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The treatment of acne and other seborrheic diseases has arisen as a significant clinical challenge due to the increasing appearance of multi-drug resistant pathogens and a high frequency of recurrent lesions. Taking into consideration the fact that some Knautia species are valuable curatives in skin diseases in traditional medicine, we assumed that the thus far unstudied species K. drymeia and K. macedonica may be a source of active substances used in skin diseases. The purpose of this study was to evaluate the antioxidant, anti-inflammatory, antibacterial, and cytotoxic activities of their extracts and fractions. An LC-MS analysis revealed the presence of 47 compounds belonging to flavonoids and phenolic acids in both species while the GC-MS procedure allowed for the identification mainly sugar derivatives, phytosterols, and fatty acids and their esters. The ethanol as well as methanol-acetone-water (3:1:1) extracts of K. drymeia (KDE and KDM) exhibited great ability to scavenge free radicals and good capacity to inhibit cyclooxygenase-1, cyclooxygenase-2, and lipoxygenase. Moreover, they had the most favorable low minimal inhibitory concentration values against acne strains, and importantly, they were non-toxic toward normal skin fibroblasts. In conclusion, K. drymeia extracts seem to be promising and safe agents for further biomedical applications.<\/jats:p>","DOI":"10.3390\/ijms24119188","type":"journal-article","created":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T02:58:48Z","timestamp":1684983528000},"page":"9188","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Anti-Acne Potential and Chemical Composition of Knautia drymeia Heuff. and Knautia macedonica Griseb Extracts"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2775-2109","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Chrz\u0105szcz","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chod\u017aki Str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0353-123X","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Miazga-Karska","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Medical University of Lublin, 1 Chod\u017aki Str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4236-9087","authenticated-orcid":false,"given":"Katarzyna","family":"Klimek","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Medical University of Lublin, 1 Chod\u017aki Str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142 P.","family":"Dybowski","sequence":"additional","affiliation":[{"name":"Department of Chromatography, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie Sklodowska University in Lublin, 20-031 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3898-8491","authenticated-orcid":false,"given":"Rafa\u0142","family":"Typek","sequence":"additional","affiliation":[{"name":"Department of Chromatography, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie Sklodowska University in Lublin, 20-031 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota","family":"Tch\u00f3rzewska","sequence":"additional","affiliation":[{"name":"Department of Plant Anatomy and Cytology, Maria Curie-Sk\u0142odowska University, Akademicka 19, 20-033 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4884-9110","authenticated-orcid":false,"given":"Katarzyna","family":"Dos Santos Szewczyk","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chod\u017aki Str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"357","DOI":"10.2165\/11632500-000000000-00000","article-title":"Newer approaches to the treatment of acne vulgaris","volume":"13","author":"Simonart","year":"2012","journal-title":"Am. J. Clin. Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Chrz\u0105szcz, M., Miazga-Karska, M., Klimek, K., Granica, S., Tch\u00f3rzewska, D., Ginalska, G., and Szewczyk, K. (2020). Extracts from Cephalaria uralensis (Murray) Roem. & Schult. and Cephalaria gigantea (Ledeb.) Bobrov as potential agents for treatment of acne vulgaris: Chemical characterization and in vitro biological evaluation. Antioxidants, 9.","DOI":"10.3390\/antiox9090796"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.det.2011.09.001","article-title":"Acne vulgaris: Pathogenesis, treatment, and needs assessment","volume":"30","author":"Dawson","year":"2012","journal-title":"Dermatol. Clin."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1046\/j.1365-4362.2000.00907.x","article-title":"Anxiety, depression, and nature of acne vulgaris in adolescents","volume":"39","author":"Aktan","year":"2000","journal-title":"Int. J. Dermatol."},{"key":"ref_5","first-page":"93","article-title":"Pathogenesis of acne vulgaris: Simplified","volume":"20","author":"Tahir","year":"2010","journal-title":"J. Pak. Assoc. Dermatol."},{"key":"ref_6","unstructured":"(2023, April 10). Global Acne Market Report for 2016\u20132026. Available online: https:\/\/www.reportlinker.com\/p05251482."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jaad.2004.01.023","article-title":"An update on the pathogenesis and management of acne vulgaris","volume":"51","author":"Harper","year":"2004","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/s00403-019-01908-x","article-title":"From pathogenesis of acne vulgaris to anti-acne agents","volume":"311","author":"Cong","year":"2019","journal-title":"Arch. Dermatol. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"27","DOI":"10.5455\/jmp.20130127102901","article-title":"Oxidative stress in acne vulgaris: An important therapeutic target","volume":"2","author":"Sahib","year":"2013","journal-title":"J. Mol. Pathophysiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1155\/MI.2005.380","article-title":"Oxidative stress in patients with acne vulgaris","volume":"6","author":"Arican","year":"2005","journal-title":"Mediators Inflamm."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Chrz\u0105szcz, M., Szewczyk, K., and Tch\u00f3rzewska, D. (2021). Biotechnological potential of Cephalaria uralensis (Murray) Roem. & Schult. And C. gigantea (Ledeb.) Bobrov\u2014Comparative analysis of plant anatomy and the content of biologically active substances. Plants, 10.","DOI":"10.3390\/plants10050986"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1056\/NEJM199805073381909","article-title":"Multidrug resistance\u2014A sign of the times","volume":"338","author":"Levy","year":"1998","journal-title":"N. Engl. J. Med."},{"key":"ref_13","first-page":"804","article-title":"Traditional medicine\u2014Inspired approaches to drug discovery: Can Ayurveda show the way forward?","volume":"14","author":"Patwardhan","year":"2009","journal-title":"Drug Discov."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.actao.2004.01.002","article-title":"Do seed mass and family affect germination and juvenile performance in Knautia arvensis? A study using failure-time methods","volume":"25","author":"Vange","year":"2004","journal-title":"Acta Oecol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1007\/s11240-017-1288-2","article-title":"The effect of cytokinins on growth, phenolics, antioxidants and antimicrobial potential in liquid agitated shoot cultures of Knautia sarajevensis","volume":"131","author":"Karalija","year":"2017","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1515\/botcro-2017-0011","article-title":"Media composition affects seed dormancy, apical dominance and phenolic profile of Knautia sarajevensis (Dipsacaceae), Bosnian endemic","volume":"77","author":"Karalija","year":"2018","journal-title":"Acta Bot. Croat."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"99","DOI":"10.24326\/asphc.2023.4913","article-title":"Comparative analysis of the biotechnological potential of Knautia drymeia Heuff. and K. macedonica Griseb","volume":"22","year":"2023","journal-title":"Acta Sci. Pol. Hortorum Cultus."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s13596-011-0006-6","article-title":"Different approaches of alternative medicines in acne vulgaris treatment","volume":"11","author":"Ghosh","year":"2011","journal-title":"Orient. Pharm. Exp. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jep.2011.08.016","article-title":"Lonicera japonica Thunb.: Ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine","volume":"138","author":"Shang","year":"2011","journal-title":"J. Ethnopharmacol."},{"key":"ref_20","unstructured":"Mugwena, N.W. (2020). A Synopsis of Medicinally Important Indigenous Species of the Genus Scabiosa (Caprifoliaceae), an Evaluation of Their Biological Activity and Synergistic Properties of Scabiosa columbaria. [Master\u2019s Thesis, University of Johannesburg]."},{"key":"ref_21","first-page":"1627","article-title":"Phenolic constituents of Knautia arvensis aerial parts","volume":"6","author":"Moldoch","year":"2011","journal-title":"Nat. Prod. Commun."},{"key":"ref_22","first-page":"545","article-title":"Variation in phenolic composition of Knautia arvensis in correlation with geographic area and plant organ","volume":"12","author":"Karalija","year":"2017","journal-title":"Nat. Prod. Commun."},{"key":"ref_23","first-page":"79","article-title":"Effects of triterpenoid saponins of field scabious (Knautia arvensis L. Coult.), alfalfa, red clover and common soapwort on growth of Gaeumannomyces graminis var. tritici and Fusarium culmorum","volume":"32","author":"Czaban","year":"2013","journal-title":"Allelopath. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.1016\/S0031-9422(00)86428-6","article-title":"Les irido\u00efdes et s\u00e9co-irido\u00efdes: R\u00e9partition, structure, propri\u00e9t\u00e9s, biosynth\u00e8se","volume":"10","author":"Plouvier","year":"1971","journal-title":"Phytochemistry"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1007\/s11094-020-02263-2","article-title":"Composition of the field scabious (Knautia arvensis L.)","volume":"54","author":"Dargaeva","year":"2020","journal-title":"Pharm. Chem. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/s11627-019-10028-0","article-title":"Harvest time-related changes in biomass, phenolics and antioxidant potential in Knautia sarajevensis shoot cultures after elicitation with salicylic acid and yeast","volume":"56","author":"Karalija","year":"2019","journal-title":"In Vitr. Cell. Dev. Biol. Plant."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1002\/jsfa.8541","article-title":"Identification and quantification of phenolic compounds in edible wild leafy vegetables by UHPLC\/Orbitrap-MS","volume":"98","author":"Giambanelli","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1134\/S1995425519060039","article-title":"Variability of chemical elements and biologically active polyphenols in Lonicera caerulea subsp. Altaica (Caprifoliaceae) plant organs along an altitudinal gradient","volume":"12","author":"Boyarskih","year":"2019","journal-title":"Contemp. Probl. Ecol."},{"key":"ref_29","first-page":"297","article-title":"Flawonoidy wybranych gatunk\u00f3w z rodzin Caprifoliaceae i Cornaceae","volume":"2","year":"1997","journal-title":"Fragm. Flor. Geobot. Ser. Polonica."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1007\/s10600-011-9954-9","article-title":"Flavonoids from Knautia montana flowers growing in Azerbaijan","volume":"47","author":"Movsumov","year":"2011","journal-title":"Chem. Nat. Compd."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5165","DOI":"10.1021\/jf010697n","article-title":"Antioxidant activity and phenolic compounds in selected herbs","volume":"49","author":"Zheng","year":"2001","journal-title":"J. Agric. Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1002\/ptr.2189","article-title":"Identification of campesterol from Chrysanthemum coronarium L. and its antiangiogenic activities","volume":"21","author":"Choi","year":"2007","journal-title":"Phytother. Res."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Chrz\u0105szcz, M., Krzemi\u0144ska, B., Celi\u0144ski, R., and Szewczyk, K. (2021). Phenolic composition and antioxidant activity of plants belonging to the Cephalaria (Caprifoliaceae) genus. Plants, 10.","DOI":"10.3390\/plants10050952"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Krzemi\u0144ska, B., Dybowski, M.P., Klimek, K., Typek, R., Miazga-Karska, M., and Dos Santos Szewczyk, K. (2022). The anti-acne potential and chemical composition of two cultivated Cotoneaster species. Cells, 11.","DOI":"10.3390\/cells11030367"},{"key":"ref_35","first-page":"101","article-title":"Preliminary anti-fungal activity of some Dipsacaceae family plants","volume":"2","author":"Kowalczyk","year":"1999","journal-title":"Herba Pol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.phytol.2011.05.006","article-title":"Isolation and characterization of biologically active glycosides from endemic Cephalaria species in Anatolia","volume":"4","author":"Pekmez","year":"2011","journal-title":"Phytochem. Lett."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1002\/cbdv.201100191","article-title":"Chemical composition and antimicrobial activity of essential oils from Scabiosa arenaria Forssk. growing wild in Tunisia","volume":"9","author":"Besbes","year":"2012","journal-title":"Chem. Biodiv."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1016\/j.carbpol.2013.03.009","article-title":"Dipsacus asperoides polysaccharide induces apoptosis in osteosarcoma cells by modulating the PI3K\/Akt pathway","volume":"95","author":"Chen","year":"2013","journal-title":"Carbohyd. Polym."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1248\/cpb.55.102","article-title":"Cytotoxic triterpenoid saponins from the roots of Cephalaria gigantea","volume":"55","author":"Tabatadze","year":"2007","journal-title":"Chem. Pharm. Bull."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1016\/j.fitote.2012.03.004","article-title":"Triterpene saponins from the roots of Dipsacus asper and their protective effects against the A\u03b225-35 induced cytotoxicity in PC12 cells","volume":"83","author":"Ji","year":"2012","journal-title":"Fitoterapia"},{"key":"ref_41","first-page":"96","article-title":"Antioxidant properties of the extracts from different parts of broccoli in Taiwan","volume":"9","author":"Guo","year":"2001","journal-title":"J. Food Drug Anal."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/ajcp\/45.4_ts.493","article-title":"Antibiotic susceptibility testing by a standardized single disk method","volume":"45","author":"Bauer","year":"1966","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_43","unstructured":"(2008). CLSI Performance Standards for Antimicrobial Susceptibility Testing, Clinical Laboratory Standards Institute. CLSI document M7-MIC."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.ijantimicag.2009.06.031","article-title":"A study of the antimicrobial activity of selected synthetic and naturally occurring quinolines","volume":"35","author":"Smyth","year":"2010","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_45","first-page":"710","article-title":"In vitro activity of peptide fractions from Impatiens glandulifera against caries causing bacteria","volume":"74","author":"Szewczyk","year":"2017","journal-title":"Acta Pol. Pharm."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1007\/s00044-016-1599-6","article-title":"Synthesis, antibacterial and antiproliferative potential of some new 1-pyridinecarbonyl-4-substituted thiosemicarbazide derivatives","volume":"25","author":"Pitucha","year":"2016","journal-title":"Med. Chem. Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/11\/9188\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:40:58Z","timestamp":1760125258000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/11\/9188"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,24]]},"references-count":46,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["ijms24119188"],"URL":"https:\/\/doi.org\/10.3390\/ijms24119188","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,24]]}}}