{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T03:00:35Z","timestamp":1782529235059,"version":"3.54.5"},"reference-count":56,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cFondo di Ateneo per la ricerca 2019, FAR 2019\u201d","award":["Rep. 2467, Prot. 94737 07\/08\/2019"],"award-info":[{"award-number":["Rep. 2467, Prot. 94737 07\/08\/2019"]}]},{"name":"\u201cFondo di Ateneo per la ricerca 2019, FAR 2019\u201d","award":["Rep. 2465, Prot. 0097985 01\/09\/2020"],"award-info":[{"award-number":["Rep. 2465, Prot. 0097985 01\/09\/2020"]}]},{"name":"\u201cFondo di Ateneo per la ricerca 2019, FAR 2019\u201d","award":["UA2002DOTTRIC2021 articolo 9, comma 3, D.M. 8 febbraio 2013, n. 45"],"award-info":[{"award-number":["UA2002DOTTRIC2021 articolo 9, comma 3, D.M. 8 febbraio 2013, n. 45"]}]},{"name":"\u201cFondo di Ateneo per la ricerca 2020, FAR 2020\u201d","award":["Rep. 2467, Prot. 94737 07\/08\/2019"],"award-info":[{"award-number":["Rep. 2467, Prot. 94737 07\/08\/2019"]}]},{"name":"\u201cFondo di Ateneo per la ricerca 2020, FAR 2020\u201d","award":["Rep. 2465, Prot. 0097985 01\/09\/2020"],"award-info":[{"award-number":["Rep. 2465, Prot. 0097985 01\/09\/2020"]}]},{"name":"\u201cFondo di Ateneo per la ricerca 2020, FAR 2020\u201d","award":["UA2002DOTTRIC2021 articolo 9, comma 3, D.M. 8 febbraio 2013, n. 45"],"award-info":[{"award-number":["UA2002DOTTRIC2021 articolo 9, comma 3, D.M. 8 febbraio 2013, n. 45"]}]},{"name":"Progetto","award":["Rep. 2467, Prot. 94737 07\/08\/2019"],"award-info":[{"award-number":["Rep. 2467, Prot. 94737 07\/08\/2019"]}]},{"name":"Progetto","award":["Rep. 2465, Prot. 0097985 01\/09\/2020"],"award-info":[{"award-number":["Rep. 2465, Prot. 0097985 01\/09\/2020"]}]},{"name":"Progetto","award":["UA2002DOTTRIC2021 articolo 9, comma 3, D.M. 8 febbraio 2013, n. 45"],"award-info":[{"award-number":["UA2002DOTTRIC2021 articolo 9, comma 3, D.M. 8 febbraio 2013, n. 45"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The spike protein (S) of SARS-CoV-2 is able to bind to the human angiotensin-converting enzyme 2 (ACE2) receptor with a much higher affinity compared to other coronaviruses. The binding interface between the ACE2 receptor and the spike protein plays a critical role in the entry mechanism of the SARS-CoV-2 virus. There are specific amino acids involved in the interaction between the S protein and the ACE2 receptor. This specificity is critical for the virus to establish a systemic infection and cause COVID-19 disease. In the ACE2 receptor, the largest number of amino acids playing a crucial role in the mechanism of interaction and recognition with the S protein is located in the C-terminal part, which represents the main binding region between ACE2 and S. This fragment is abundant in coordination residues such as aspartates, glutamates, and histidine that could be targeted by metal ions. Zn2+ ions bind to the ACE2 receptor in its catalytic site and modulate its activity, but it could also contribute to the structural stability of the entire protein. The ability of the human ACE2 receptor to coordinate metal ions, such as Zn2+, in the same region where it binds to the S protein could have a crucial impact on the mechanism of recognition and interaction of ACE2\u2013S, with consequences on their binding affinity that deserve to be investigated. To test this possibility, this study aims to characterize the coordination ability of Zn2+, and also Cu2+ for comparison, with selected peptide models of the ACE2 binding interface using spectroscopic and potentiometric techniques.<\/jats:p>","DOI":"10.3390\/ijms24119202","type":"journal-article","created":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T02:58:48Z","timestamp":1684983528000},"page":"9202","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Zn2+ and Cu2+ Interaction with the Recognition Interface of ACE2 for SARS-CoV-2 Spike Protein"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9502-7585","authenticated-orcid":false,"given":"Alessio","family":"Pelucelli","sequence":"first","affiliation":[{"name":"Department of Chemical, Physical, Mathematical and Natural Sciences, University of Sassari, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3306-0419","authenticated-orcid":false,"given":"Massimiliano","family":"Peana","sequence":"additional","affiliation":[{"name":"Department of Chemical, Physical, Mathematical and Natural Sciences, University of Sassari, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartosz","family":"Orze\u0142","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, University of Wroclaw, 50-383 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Piasta","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, University of Wroclaw, 50-383 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9556-6378","authenticated-orcid":false,"given":"Elzbieta","family":"Gumienna-Kontecka","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, University of Wroclaw, 50-383 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4304-0251","authenticated-orcid":false,"given":"Serenella","family":"Medici","sequence":"additional","affiliation":[{"name":"Department of Chemical, Physical, Mathematical and Natural Sciences, University of Sassari, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9583-4750","authenticated-orcid":false,"given":"Maria Antonietta","family":"Zoroddu","sequence":"additional","affiliation":[{"name":"Department of Chemical, Physical, Mathematical and Natural Sciences, University of Sassari, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1186\/s13054-020-03120-0","article-title":"Role of angiotensin-converting enzyme 2 (ACE2) in COVID-19","volume":"24","author":"Ni","year":"2020","journal-title":"Crit. 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