{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T16:30:14Z","timestamp":1772814614175,"version":"3.50.1"},"reference-count":41,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T00:00:00Z","timestamp":1685059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>TRPC channels are critical players in cochlear hair cells and sensory neurons, as demonstrated in animal experiments. However, evidence for TRPC expression in the human cochlea is still lacking. This reflects the logistic and practical difficulties in obtaining human cochleae. The purpose of this study was to detect TRPC6, TRPC5 and TRPC3 in the human cochlea. Temporal bone pairs were excised from ten body donors, and the inner ear was first assessed based on computed tomography scans. Decalcification was then performed using 20% EDTA solutions. Immunohistochemistry with knockout-tested antibodies followed. The organ of Corti, the stria vascularis, the spiral lamina, spiral ganglion neurons and cochlear nerves were specifically stained. This unique report of TRPC channels in the human cochlea supports the hypothesis of the potentially critical role of TRPC channels in human cochlear health and disease which has been suggested in previous rodent experiments.<\/jats:p>","DOI":"10.3390\/ijms24119290","type":"journal-article","created":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T02:05:44Z","timestamp":1685066744000},"page":"9290","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Immunohistochemistry Reveals TRPC Channels in the Human Hearing Organ\u2014A Novel CT-Guided Approach to the Cochlea"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6746-3681","authenticated-orcid":false,"given":"Colya N.","family":"Englisch","sequence":"first","affiliation":[{"name":"Institute of Anatomy and Cell Biology, Saarland University, 66421 Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakob","family":"Steinh\u00e4user","sequence":"additional","affiliation":[{"name":"Institute of Anatomy and Cell Biology, Saarland University, 66421 Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7109-5188","authenticated-orcid":false,"given":"Silke","family":"Wemmert","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery, Saarland University, 66421 Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1482-7020","authenticated-orcid":false,"given":"Martin","family":"Jung","sequence":"additional","affiliation":[{"name":"Institute of Medical Biochemistry and Molecular Biology, Saarland University, 66421 Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua","family":"Gawlitza","sequence":"additional","affiliation":[{"name":"Institute of Radiology, Technical University of Munich, 80333 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gentiana","family":"Wenzel","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery, Saarland University, 66421 Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernhard","family":"Schick","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery, Saarland University, 66421 Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7788-1796","authenticated-orcid":false,"given":"Thomas","family":"Tschernig","sequence":"additional","affiliation":[{"name":"Institute of Anatomy and Cell Biology, Saarland University, 66421 Homburg\/Saar, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/j.1749-6632.1991.tb19572.x","article-title":"Genetic Epidemiology of Hearing Impairment","volume":"630","author":"Morton","year":"1991","journal-title":"Ann. 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