{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:10:45Z","timestamp":1760148645689,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T00:00:00Z","timestamp":1685059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Shandong Province in China","award":["ZR2021MB024","21778047","21675138","21705139"],"award-info":[{"award-number":["ZR2021MB024","21778047","21675138","21705139"]}]},{"name":"National Natural Science Foundation of China","award":["ZR2021MB024","21778047","21675138","21705139"],"award-info":[{"award-number":["ZR2021MB024","21778047","21675138","21705139"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In this paper, glutathione (GSH)-coated Zn-doped CdTe quantum dots (QDs) with different particle sizes were synthesized using the \u201creflow method\u201d, and the interaction mechanism between the two QDs and lactoferrin (LF) was investigated systemically with different spectroscopic methods. The steady-state fluorescence spectra showed that the LF formed a tight complex with the two QDs through static bursting and that the electrostatic force was the main driving force between the two LF\u2013QDs systems. The complex generation process was found to be spontaneous (\u0394G &lt; 0) and accompanied by exothermic and increasing degrees of freedom (\u0394H &lt; 0, \u0394S &gt; 0) by using the temperature-dependent fluorescence spectroscopy. The critical transfer distance (R0) and donor\u2013acceptor distance (r) of the two LF\u2013QDs systems were obtained based on the fluorescence resonance energy transfer theory. In addition, it was observed that the QDs changed the secondary and tertiary structures of LF, leading to an increase in the hydrophobicity of LF. Further, the nano-effect of orange QDs on LF is much larger than that of green QDs. The above results provide a basis for metal-doped QDs with LF in safe nano-bio applications.<\/jats:p>","DOI":"10.3390\/ijms24119325","type":"journal-article","created":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T16:19:37Z","timestamp":1685204377000},"page":"9325","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Comparative Study of Nanobio Interaction of Zn-Doped CdTe Quantum Dots with Lactoferrin Using Different Spectroscopic Methods"],"prefix":"10.3390","volume":"24","author":[{"given":"Meng","family":"Ji","sequence":"first","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liwei","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyuan","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8466-5071","authenticated-orcid":false,"given":"Xuming","family":"Zhuang","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Luan","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10939","DOI":"10.1021\/jacs.7b05267","article-title":"Chemical synthesis and luminescence applications of colloidal semiconductor quantum dots","volume":"139","author":"Owen","year":"2017","journal-title":"J. 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