{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T07:34:35Z","timestamp":1769153675757,"version":"3.49.0"},"reference-count":59,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T00:00:00Z","timestamp":1685404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["703143"],"award-info":[{"award-number":["703143"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Beckley Foundation","award":["703143"],"award-info":[{"award-number":["703143"]}]},{"name":"National Institute of Health","award":["703143"],"award-info":[{"award-number":["703143"]}]},{"name":"Medical Research Council","award":["703143"],"award-info":[{"award-number":["703143"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>(1) From mouse to man, shaking behavior (head twitches and\/or wet dog shakes) is a reliable readout of psychedelic drug action. Shaking behavior like psychedelia is thought to be mediated by serotonin 2A receptors on cortical pyramidal cells. The involvement of pyramidal cells in psychedelic-induced shaking behavior remains hypothetical, though, as experimental in vivo evidence is limited. (2) Here, we use cell type-specific voltage imaging in awake mice to address this issue. We intersectionally express the genetically encoded voltage indicator VSFP Butterfly 1.2 in layer 2\/3 pyramidal neurons. We simultaneously capture cortical hemodynamics and cell type-specific voltage activity while mice display psychedelic shaking behavior. (3) Shaking behavior is preceded by high-frequency oscillations and overlaps with low-frequency oscillations in the motor cortex. Oscillations spectrally mirror the rhythmics of shaking behavior and reflect layer 2\/3 pyramidal cell activity complemented by hemodynamics. (4) Our results reveal a clear cortical fingerprint of serotonin-2A-receptor-mediated shaking behavior and open a promising methodological avenue relating a cross-mammalian psychedelic effect to cell-type specific brain dynamics.<\/jats:p>","DOI":"10.3390\/ijms24119463","type":"journal-article","created":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T02:33:27Z","timestamp":1685414007000},"page":"9463","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Cortical Correlates of Psychedelic-Induced Shaking Behavior Revealed by Voltage Imaging"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0538-5184","authenticated-orcid":false,"given":"Tobias","family":"Buchborn","sequence":"first","affiliation":[{"name":"Laboratory for Neuronal Circuit Dynamics, Imperial College London, London W12 0NN, UK"},{"name":"Institute of Psychopharmacology, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, 69115 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3118-7344","authenticated-orcid":false,"given":"Taylor","family":"Lyons","sequence":"additional","affiliation":[{"name":"Laboratory for Neuronal Circuit Dynamics, Imperial College London, London W12 0NN, UK"},{"name":"Centre for Psychedelic Research, Department of Medicine, Imperial College London, London W12 0NN, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4574-6431","authenticated-orcid":false,"given":"Chenchen","family":"Song","sequence":"additional","affiliation":[{"name":"Laboratory for Neuronal Circuit Dynamics, Imperial College London, London W12 0NN, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda","family":"Feilding","sequence":"additional","affiliation":[{"name":"The Beckley Foundation, Beckley Park, Oxford OX3 9SY, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5718-0765","authenticated-orcid":false,"given":"Thomas","family":"Kn\u00f6pfel","sequence":"additional","affiliation":[{"name":"Laboratory for Neuronal Circuit Dynamics, Imperial College London, London W12 0NN, UK"},{"name":"Centre for Neurotechnology, Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, UK"},{"name":"Laboratory for Neuronal Circuit Dynamics, Hong Kong Baptist University, Kowloon Tong, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.bbr.2014.12.014","article-title":"Tolerance to LSD and DOB induced Shaking behaviour: Differential adaptations of frontocortical 5-HT2A and glutamate receptor binding sites","volume":"281","author":"Buchborn","year":"2015","journal-title":"Behav. 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