{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:13:37Z","timestamp":1790734417408,"version":"4.1.0"},"reference-count":36,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T00:00:00Z","timestamp":1685404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University Hospital of RWTH Aachen","award":["START  16\/22"],"award-info":[{"award-number":["START  16\/22"]}]},{"name":"University Hospital of RWTH Aachen","award":["#80-17"],"award-info":[{"award-number":["#80-17"]}]},{"name":"University Hospital of RWTH Aachen","award":["ERA-NET NEURON, 01EW1906A"],"award-info":[{"award-number":["ERA-NET NEURON, 01EW1906A"]}]},{"name":"Swiss Anorexia Nervosa Society","award":["START  16\/22"],"award-info":[{"award-number":["START  16\/22"]}]},{"name":"Swiss Anorexia Nervosa Society","award":["#80-17"],"award-info":[{"award-number":["#80-17"]}]},{"name":"Swiss Anorexia Nervosa Society","award":["ERA-NET NEURON, 01EW1906A"],"award-info":[{"award-number":["ERA-NET NEURON, 01EW1906A"]}]},{"DOI":"10.13039\/501100002347","name":"Federal Ministry of Education and Research (BMBF), together with the European Union","doi-asserted-by":"publisher","award":["START  16\/22"],"award-info":[{"award-number":["START  16\/22"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Federal Ministry of Education and Research (BMBF), together with the European Union","doi-asserted-by":"publisher","award":["#80-17"],"award-info":[{"award-number":["#80-17"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Federal Ministry of Education and Research (BMBF), together with the European Union","doi-asserted-by":"publisher","award":["ERA-NET NEURON, 01EW1906A"],"award-info":[{"award-number":["ERA-NET NEURON, 01EW1906A"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The gut microbiota composition is causally involved in the regulation of body weight. Through the gut\u2013brain axis, microbiota play a role in psychiatric disorders including anorexia nervosa (AN). Previously, we showed microbiome changes to be associated with brain volume and astrocyte reductions after chronic starvation in an AN animal model. Here, we analyzed whether these alterations are reversible after refeeding. The activity-based anorexia (ABA) model is a well-established animal model that mimics several symptoms of AN. Fecal samples and the brain were analyzed. Like previous results, significant alterations in the microbiome were observed after starvation. After refeeding, including the normalization of food intake and body weight, \u03b1- and \u03b2-diversity, as well as the relative abundance of specific genera, were largely normalized in starved rats. Brain parameters appeared to normalize alongside microbial restitution with some aberrations in the white matter. We confirmed our previous findings of microbial dysbiosis during starvation and showed a high degree of reversibility. Thus, microbiome alterations in the ABA model appear to be mostly starvation-related. These findings support the usefulness of the ABA model in investigating starvation-induced effects on the microbiota\u2013gut\u2013brain axis to help comprehend the pathomechanisms of AN and potentially develop microbiome-targeted treatments for patients.<\/jats:p>","DOI":"10.3390\/ijms24119496","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T04:40:51Z","timestamp":1685508051000},"page":"9496","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Gut Microbiota and Brain Alterations after Refeeding in a Translational Anorexia Nervosa Rat Model"],"prefix":"10.3390","volume":"24","author":[{"given":"Stefanie","family":"Trinh","sequence":"first","affiliation":[{"name":"Institute of Neuroanatomy, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Kogel","sequence":"additional","affiliation":[{"name":"Institute of Neuroanatomy, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilly","family":"Kneisel","sequence":"additional","affiliation":[{"name":"Institute of Neuroanatomy, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"M\u00fcller-Limberger","sequence":"additional","affiliation":[{"name":"Institute of Neuroanatomy, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8450-3323","authenticated-orcid":false,"given":"Beate","family":"Herpertz-Dahlmann","sequence":"additional","affiliation":[{"name":"Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, RWTH Aachen University, Neuenhofer Weg 21, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3926-2553","authenticated-orcid":false,"given":"Cordian","family":"Beyer","sequence":"additional","affiliation":[{"name":"Institute of Neuroanatomy, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0110-7980","authenticated-orcid":false,"given":"Jochen","family":"Seitz","sequence":"additional","affiliation":[{"name":"Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, RWTH Aachen University, Neuenhofer Weg 21, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"APA (2013). Diagnostic and Statistical Manual of Mental Disorders, American Psychiatric Pub Inc.. [5th ed.].","DOI":"10.1176\/appi.books.9780890425596"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1192\/bjp.2019.113","article-title":"Anorexia nervosa: 30-year outcome","volume":"216","author":"Dobrescu","year":"2020","journal-title":"Br. J. Psychiatry J. Ment. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1097\/YCO.0000000000000739","article-title":"Incidence, prevalence and mortality of anorexia nervosa and bulimia nervosa","volume":"34","author":"Hoek","year":"2021","journal-title":"Curr. Opin. Psychiatry"},{"key":"ref_4","first-page":"5821","article-title":"Long-term benefit of Microbiota Transfer Therapy on autism symptoms and gut microbiota","volume":"9","author":"Kang","year":"2019","journal-title":"Nature"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.jad.2016.05.038","article-title":"Possible association of Bifidobacterium and Lactobacillus in the gut microbiota of patients with major depressive disorder","volume":"202","author":"Aizawa","year":"2016","journal-title":"J. Affect. Disord."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.bbi.2015.03.016","article-title":"Altered fecal microbiota composition in patients with major depressive disorder","volume":"48","author":"Jiang","year":"2015","journal-title":"Brain Behav. Immun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1097\/PSY.0000000000000247","article-title":"The Intestinal Microbiota in Acute Anorexia Nervosa and During Renourishment: Relationship to Depression, Anxiety, and Eating Disorder Psychopathology","volume":"77","author":"Kleiman","year":"2015","journal-title":"Psychosom. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.2174\/1570159X16666180118101354","article-title":"Is the Impact of Starvation on the Gut Microbiota Specific or Unspecific to Anorexia Nervosa? A Narrative Review Based on a Systematic Literature Search","volume":"16","author":"Mack","year":"2018","journal-title":"Curr. Neuropharmacol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1002\/eat.23435","article-title":"Gut microbiota alteration in adolescent anorexia nervosa does not normalize with short-term weight restoration","volume":"54","author":"Schulz","year":"2021","journal-title":"Int. J. Eat. Disord."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"110114","DOI":"10.1016\/j.pnpbp.2020.110114","article-title":"Anorexia nervosa and gut microbiota: A systematic review and quantitative synthesis of pooled microbiological data","volume":"106","author":"Mondot","year":"2021","journal-title":"Prog. Neuro-Psychopharmacol. Biol. Psychiatry"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"G1288","DOI":"10.1152\/ajpgi.00341.2012","article-title":"Critical role of gut microbiota in the production of biologically active, free catecholamines in the gut lumen of mice. American journal of physiology","volume":"303","author":"Asano","year":"2012","journal-title":"Gastrointest. Liver Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/nrendo.2016.150","article-title":"Role of the gut microbiota in host appetite control: Bacterial growth to animal feeding behaviour","volume":"13","author":"Fetissov","year":"2017","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1002\/eat.22801","article-title":"Gut microbiota and body composition in anorexia nervosa inpatients in comparison to athletes, overweight, obese, and normal weight controls","volume":"50","author":"Morkl","year":"2017","journal-title":"Int. J. Eat. Disord."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1241214","DOI":"10.1126\/science.1241214","article-title":"Gut microbiota from twins discordant for obesity modulate metabolism in mice","volume":"341","author":"Ridaura","year":"2013","journal-title":"Science"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.clnu.2020.05.002","article-title":"Gut microbiota alteration in a mouse model of Anorexia Nervosa","volume":"40","author":"Breton","year":"2021","journal-title":"Clin. Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Queipo-Ortuno, M.I., Seoane, L.M., Murri, M., Pardo, M., Gomez-Zumaquero, J.M., Cardona, F., Casanueva, F., and Tinahones, F.J. (2013). Gut microbiota composition in male rat models under different nutritional status and physical activity and its association with serum leptin and ghrelin levels. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0065465"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.jpsychires.2020.12.030","article-title":"Gut microbiota and brain alterations in a translational anorexia nervosa rat model","volume":"133","author":"Trinh","year":"2021","journal-title":"J. Psychiatr. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2441","DOI":"10.1210\/en.2019-00408","article-title":"The Gut Microbiome Derived from Anorexia Nervosa Patients Impairs Weight Gain and Behavioral Performance in Female Mice","volume":"160","author":"Hata","year":"2019","journal-title":"Endocrinology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1126\/science.1229000","article-title":"Gut microbiomes of Malawian twin pairs discordant for kwashiorkor","volume":"339","author":"Smith","year":"2013","journal-title":"Science"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1897216","DOI":"10.1080\/19490976.2021.1897216","article-title":"Gut microbial communities from patients with anorexia nervosa do not influence body weight in recipient germ-free mice","volume":"13","author":"Glenny","year":"2021","journal-title":"Gut Microbes"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1016\/j.jaac.2022.03.026","article-title":"Dynamic Structural Brain Changes in Anorexia Nervosa: A Replication Study, Mega-analysis, and Virtual Histology Approach","volume":"61","author":"Bahnsen","year":"2022","journal-title":"J. Am. Acad. Child Adolesc. Psychiatry"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.2174\/1570159X15666171109145651","article-title":"Extend, Pathomechanism and Clinical Consequences of Brain Volume Changes in Anorexia Nervosa","volume":"16","author":"Seitz","year":"2018","journal-title":"Curr. Neuropharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1038\/s41398-019-0493-7","article-title":"The reduction of astrocytes and brain volume loss in anorexia nervosa-the impact of starvation and refeeding in a rodent model","volume":"9","author":"Frintrop","year":"2019","journal-title":"Transl. Psychiatry"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"105666","DOI":"10.1016\/j.appet.2021.105666","article-title":"Adolescent female rats prone to the activity based anorexia (ABA) paradigm have altered hedonic responses and cortical astrocyte density compared to resistant animals","volume":"168","author":"Hurley","year":"2022","journal-title":"Appetite"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Gabloffsky, T., and Gill, S. (2022). Food Restriction in Mice Induces Food-Anticipatory Activity and Circadian-Rhythm-Related Activity Changes. Nutrients, 14.","DOI":"10.3390\/nu14245252"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"26752","DOI":"10.1038\/srep26752","article-title":"Weight gain in anorexia nervosa does not ameliorate the faecal microbiota, branched chain fatty acid profiles, and gastrointestinal complaints","volume":"6","author":"Mack","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Morita, C., Tsuji, H., Hata, T., Gondo, M., Takakura, S., Kawai, K., Yoshihara, K., Ogata, K., Nomoto, K., and Miyazaki, K. (2015). Gut Dysbiosis in Patients with Anorexia Nervosa. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0145274"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1007\/s00394-018-1732-z","article-title":"Influence of 4-week multi-strain probiotic administration on resting-state functional connectivity in healthy volunteers","volume":"58","author":"Bagga","year":"2019","journal-title":"Eur. J. Nutr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"596381","DOI":"10.3389\/fnhum.2020.596381","article-title":"Animal Models for Anorexia Nervosa-A Systematic Review","volume":"14","author":"Scharner","year":"2020","journal-title":"Front. Hum. Neurosci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"43","DOI":"10.3389\/fnins.2020.00043","article-title":"Rethinking Therapeutic Strategies for Anorexia Nervosa: Insights from Psychedelic Medicine and Animal Models","volume":"14","author":"Foldi","year":"2020","journal-title":"Front. Neurosci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"711181","DOI":"10.3389\/fpsyt.2021.711181","article-title":"The Utility of Animal Models for Studying the Metabo-Psychiatric Origins of Anorexia Nervosa","volume":"12","author":"Zhang","year":"2021","journal-title":"Front. Psychiatry"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"33721","DOI":"10.1038\/srep33721","article-title":"IMNGS: A comprehensive open resource of processed 16S rRNA microbial profiles for ecology and diversity studies","volume":"6","author":"Lagkouvardos","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e2836","DOI":"10.7717\/peerj.2836","article-title":"Rhea: A transparent and modular R pipeline for microbial profiling based on 16S rRNA gene amplicons","volume":"5","author":"Lagkouvardos","year":"2017","journal-title":"PeerJ"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1080\/15622975.2016.1273552","article-title":"Reduced astrocyte density underlying brain volume reduction in activity-based anorexia rats","volume":"9","author":"Frintrop","year":"2018","journal-title":"World J. Biol. Psychiatry"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/7854_2010_77","article-title":"Neurobiology driving hyperactivity in activity-based anorexia","volume":"6","author":"Adan","year":"2011","journal-title":"Curr. Top. Behav. Neurosci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.jneumeth.2017.09.018","article-title":"Establishment of a chronic activity-based anorexia rat model","volume":"293","author":"Frintrop","year":"2018","journal-title":"J. Neurosci. Methods"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/11\/9496\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:45:17Z","timestamp":1760125517000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/11\/9496"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,30]]},"references-count":36,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["ijms24119496"],"URL":"https:\/\/doi.org\/10.3390\/ijms24119496","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,30]]}}}