{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T22:42:35Z","timestamp":1785883355422,"version":"3.56.0"},"reference-count":100,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T00:00:00Z","timestamp":1685491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013302","name":"King Adullah International Medical Research Center","doi-asserted-by":"publisher","award":["RC20\/346"],"award-info":[{"award-number":["RC20\/346"]}],"id":[{"id":"10.13039\/501100013302","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Among the newer choices of targeted therapies against cancer, stem cell therapy is gaining importance because of their antitumor properties. Stem cells suppress growth, metastasis, and angiogenesis, and induce apoptosis in cancer cells. In this study, we have examined the impact of the cellular component and the secretome of preconditioned and na\u00efve placenta-derived Chorionic Villus Mesenchymal Stem Cells (CVMSCs) on the functional characteristics of the Human Breast Cancer cell line MDA231. MDA231 cells were treated with preconditioned CVMSCs and their conditioned media (CM), followed by an evaluation of their functional activities and modulation in gene and protein expression. Human Mammary Epithelial Cells (HMECs) were used as a control. CM obtained from the preconditioned CVMSCs significantly altered the proliferation of MDA231 cells, yet no change in other phenotypes, such as adhesion, migration, and invasion, were observed at various concentrations and time points tested. However, the cellular component of preconditioned CVMSCs significantly inhibited several phenotypes of MDA231 cells, including proliferation, migration, and invasion. CVMSCs-treated MDA231 cells exhibited modulation in the expression of various genes involved in apoptosis, oncogenesis, and Epithelial to Mesenchymal Transition (EMT), explaining the changes in the invasive behavior of MDA231 cells. These studies reveal that preconditioned CVMSCs may make useful candidate in a stem cell-based therapy against cancer.<\/jats:p>","DOI":"10.3390\/ijms24119569","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T03:06:00Z","timestamp":1685588760000},"page":"9569","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Preconditioned Chorionic Villus Mesenchymal Stem\/Stromal Cells (CVMSCs) Minimize the Invasive Phenotypes of Breast Cancer Cell Line MDA231 In Vitro"],"prefix":"10.3390","volume":"24","author":[{"given":"Abdullah Al","family":"Subayyil","sequence":"first","affiliation":[{"name":"Blood and Cancer Research Department, King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences (KSAU), Ministry of National Guard Health Affairs (MNGHA), Riyadh 11426, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasser S.","family":"Basmaeil","sequence":"additional","affiliation":[{"name":"Blood and Cancer Research Department, King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences (KSAU), Ministry of National Guard Health Affairs (MNGHA), Riyadh 11426, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayaa Bin","family":"Kulayb","sequence":"additional","affiliation":[{"name":"Blood and Cancer Research Department, King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences (KSAU), Ministry of National Guard Health Affairs (MNGHA), Riyadh 11426, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha","family":"Alrodayyan","sequence":"additional","affiliation":[{"name":"Blood and Cancer Research Department, King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences (KSAU), Ministry of National Guard Health Affairs (MNGHA), Riyadh 11426, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lama Abdulaziz A.","family":"Alhaber","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taghreed N.","family":"Almanaa","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9320-5579","authenticated-orcid":false,"given":"Tanvir","family":"Khatlani","sequence":"additional","affiliation":[{"name":"Blood and Cancer Research Department, King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences (KSAU), Ministry of National Guard Health Affairs (MNGHA), Riyadh 11426, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1097\/JTO.0b013e3181c6f035","article-title":"First-Line Systemic Chemotherapy in the Treatment of Advanced Non-Small Cell Lung Cancer: A Systematic Review","volume":"5","author":"Goffin","year":"2010","journal-title":"J. Thorac. Oncol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1038\/nrc3775","article-title":"Non-Small-Cell Lung Cancers: A Heterogeneous Set of Diseases","volume":"14","author":"Chen","year":"2014","journal-title":"Nat. Rev. Cancer"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1002\/hon.2591","article-title":"CAR T-Cell Therapy: Full Speed Ahead","volume":"37","author":"Sermer","year":"2019","journal-title":"Hematol. Oncol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1038\/d41586-018-06756-9","article-title":"Clear up This Stem-Cell Mess","volume":"561","author":"Sipp","year":"2018","journal-title":"Nature"},{"key":"ref_6","first-page":"4747","article-title":"Deepening a Simple Question: Can MSCs Be Used to Treat Cancer?","volume":"37","author":"Gomes","year":"2017","journal-title":"Anticancer Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"840","DOI":"10.3389\/fonc.2019.00840","article-title":"New Insights Into Implementation of Mesenchymal Stem Cells in Cancer Therapy: Prospects for Anti-Angiogenesis Treatment","volume":"9","author":"Javan","year":"2019","journal-title":"Front. Oncol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"461718","DOI":"10.1155\/2012\/461718","article-title":"Mesenchymal Stem Cells Isolated from Adipose and Other Tissues: Basic Biological Properties and Clinical Applications","volume":"2012","author":"Orbay","year":"2012","journal-title":"Stem Cells Int."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.jcyt.2012.11.005","article-title":"Mesenchymal Stromal Cells: Misconceptions and Evolving Concepts","volume":"15","author":"Phinney","year":"2013","journal-title":"Cytotherapy"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1634\/stemcells.22-3-377","article-title":"Mesenchymal Stem Cells Can Be Differentiated into Endothelial Cells in Vitro","volume":"22","author":"Oswald","year":"2004","journal-title":"Stem Cells"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/978-3-030-37184-5_3","article-title":"Mesenchymal Stem Cells in the Tumor Microenvironment","volume":"1234","author":"Atiya","year":"2020","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1038\/cr.2008.40","article-title":"Suppression of Tumorigenesis by Human Mesenchymal Stem Cells in a Hepatoma Model","volume":"18","author":"Qiao","year":"2008","journal-title":"Cell Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1007\/s000180050322","article-title":"Factor(s) from Nonmacrophage Bone Marrow Stromal Cells Inhibit Lewis Lung Carcinoma and B16 Melanoma Growth in Mice","volume":"55","author":"Maestroni","year":"1999","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1038\/sj.gt.3302276","article-title":"Antitumor Effect of Genetically Engineered Mesenchymal Stem Cells in a Rat Glioma Model","volume":"11","author":"Nakamura","year":"2004","journal-title":"Gene Ther."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.cellbi.2007.08.002","article-title":"Human Mesenchymal Stem Cells Isolated from the Umbilical Cord","volume":"32","author":"Qiao","year":"2008","journal-title":"Cell Biol. Int."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4197","DOI":"10.1182\/blood-2008-09-176198","article-title":"Concentration-Dependent Inhibition of Angiogenesis by Mesenchymal Stem Cells","volume":"113","author":"Otsu","year":"2009","journal-title":"Blood"},{"key":"ref_17","first-page":"453","article-title":"Human Umbilical Cord Blood Stem Cells Show PDGF-D-Dependent Glioma Cell Tropism in Vitro and in Vivo","volume":"12","author":"Gondi","year":"2010","journal-title":"Neuro. Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.1089\/scd.2012.0486","article-title":"Umbilical Cord Blood-Derived Mesenchymal Stem Cells Inhibit, but Adipose Tissue-Derived Mesenchymal Stem Cells Promote, Glioblastoma Multiforme Proliferation","volume":"22","author":"Akimoto","year":"2013","journal-title":"Stem Cells Dev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5184601","DOI":"10.1155\/2016\/5184601","article-title":"Phenotypic and Functional Characterization of Mesenchymal Stem\/Multipotent Stromal Cells from Decidua Basalis of Human Term Placenta","volume":"2016","author":"Abomaray","year":"2016","journal-title":"Stem Cells Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1007\/s12015-012-9385-4","article-title":"Phenotypic and Functional Characterization of Mesenchymal Stem Cells from Chorionic Villi of Human Term Placenta","volume":"9","author":"Abumaree","year":"2013","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1177\/1933719116632924","article-title":"Phenotypic and Functional Characterization of Mesenchymal Stem\/Multipotent Stromal Cells From Decidua Parietalis of Human Term Placenta","volume":"23","author":"Abumaree","year":"2016","journal-title":"Reprod. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.placenta.2017.04.003","article-title":"Immunomodulatory Properties of Human Placental Mesenchymal Stem\/Stromal Cells","volume":"59","author":"Abumaree","year":"2017","journal-title":"Placenta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"650125","DOI":"10.3389\/fcell.2021.650125","article-title":"Conditions Mimicking the Cancer Microenvironment Modulate the Functional Outcome of Human Chorionic Villus Mesenchymal Stem\/Stromal Cells in Vitro","volume":"9","author":"Basmaeil","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1002\/jgm.452","article-title":"Mesenchymal Stem Cells Home to Injured Tissues When Co-Infused with Hematopoietic Cells to Treat a Radiation-Induced Multi-Organ Failure Syndrome","volume":"5","author":"Chapel","year":"2003","journal-title":"J. Gene Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1002\/path.2469","article-title":"Why Are MSCs Therapeutic? New Data: New Insight","volume":"217","author":"Caplan","year":"2009","journal-title":"J. Pathol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1038\/nri3209","article-title":"Multipotent Mesenchymal Stromal Cells and the Innate Immune System","volume":"12","author":"Mougiakakos","year":"2012","journal-title":"Nat. Rev. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1146\/annurev-pathol-011110-130230","article-title":"Mesenchymal Stem Cells: Mechanisms of Inflammation","volume":"6","author":"Singer","year":"2011","journal-title":"Annu. Rev. Pathol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.pharmthera.2014.02.013","article-title":"Mesenchymal Stem\/Stromal Cells as a Pharmacological and Therapeutic Approach to Accelerate Angiogenesis","volume":"143","author":"Bronckaers","year":"2014","journal-title":"Pharmacol. Ther."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1146\/annurev-immunol-032712-095919","article-title":"Mesenchymal Stem Cell: Keystone of the Hematopoietic Stem Cell Niche and a Stepping-Stone for Regenerative Medicine","volume":"31","author":"Frenette","year":"2013","journal-title":"Annu. Rev. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"9298","DOI":"10.3390\/ijms13079298","article-title":"The Immunomodulatory and Neuroprotective Effects of Mesenchymal Stem Cells (MSCs) in Experimental Autoimmune Encephalomyelitis (EAE): A Model of Multiple Sclerosis (MS)","volume":"13","author":"Abumaree","year":"2012","journal-title":"Int. J. Mol. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1007\/s12015-013-9455-2","article-title":"Human Placental Mesenchymal Stem Cells (PMSCs) Play a Role as Immune Suppressive Cells by Shifting Macrophage Differentiation from Inflammatory M1 to Anti-Inflammatory M2 Macrophages","volume":"9","author":"Abumaree","year":"2013","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.placenta.2017.05.001","article-title":"Human Chorionic Villous Mesenchymal Stem\/Stromal Cells Modify the Effects of Oxidative Stress on Endothelial Cell Functions","volume":"59","author":"Abumaree","year":"2017","journal-title":"Placenta"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1186\/s13287-018-0984-0","article-title":"Human Chorionic Villous Mesenchymal Stem\/Stromal Cells Protect Endothelial Cells from Injury Induced by High Level of Glucose","volume":"9","author":"Basmaeil","year":"2018","journal-title":"Stem Cell Res. Ther."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/s13287-019-1153-9","article-title":"Preconditioning Human Natural Killer Cells with Chorionic Villous Mesenchymal Stem Cells Stimulates Their Expression of Inflammatory and Anti-Tumor Molecules","volume":"10","author":"Abumaree","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"54","DOI":"10.4252\/wjsc.v14.i1.54","article-title":"Modified Mesenchymal Stem Cells in Cancer Therapy: A Smart Weapon Requiring Upgrades for Wider Clinical Applications","volume":"14","author":"Vicinanza","year":"2022","journal-title":"World J. Stem Cells"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1038\/leu.2011.108","article-title":"Mesenchymal Stromal Cell \u201cLicensing\u201d: A Multistep Process","volume":"25","author":"Krampera","year":"2011","journal-title":"Leukemia"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1007\/s12013-011-9317-y","article-title":"TNF-\u03b1 Increases Bone Marrow Mesenchymal Stem Cell Migration to Ischemic Tissues","volume":"62","author":"Xiao","year":"2012","journal-title":"Cell Biochem. Biophys."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1634\/stemcells.2007-0054","article-title":"The in Vitro Migration Capacity of Human Bone Marrow Mesenchymal Stem Cells: Comparison of Chemokine and Growth Factor Chemotactic Activities","volume":"25","author":"Ponte","year":"2007","journal-title":"Stem Cells"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1093\/jnen\/nlz016","article-title":"Transforming Growth Factor-\u03b2 Promotes Homing and Therapeutic Efficacy of Human Mesenchymal Stem Cells to Glioblastoma","volume":"78","author":"Li","year":"2019","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"29034","DOI":"10.18632\/oncotarget.4732","article-title":"IL-1\u03b2 Produced by Aggressive Breast Cancer Cells Is One of the Factors That Dictate Their Interactions with Mesenchymal Stem Cells through Chemokine Production","volume":"6","author":"Escobar","year":"2015","journal-title":"Oncotarget"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Lieblein, J.C., Ball, S., Hutzen, B., Sasser, A.K., Lin, H.-J., Huang, T.H., Hall, B.M., and Lin, J. (2008). STAT3 Can Be Activated through Paracrine Signaling in Breast Epithelial Cells. BMC Cancer, 8.","DOI":"10.1186\/1471-2407-8-302"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1038\/sj.onc.1206113","article-title":"IL-8 Expression and Its Possible Relationship with Estrogen-Receptor-Negative Status of Breast Cancer Cells","volume":"22","author":"Freund","year":"2003","journal-title":"Oncogene"},{"key":"ref_43","first-page":"3821","article-title":"Matrix Metalloproteinases (MMPs) in Breast Cancer Cell Lines of Different Tumorigenicity","volume":"21","author":"Bachmeier","year":"2001","journal-title":"Anticancer Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"41067","DOI":"10.1038\/srep41067","article-title":"IL-1\u03b2 Induces Apoptosis and Autophagy via Mitochondria Pathway in Human Degenerative Nucleus Pulposus Cells","volume":"7","author":"Shen","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3958069","DOI":"10.1155\/2017\/3958069","article-title":"Dual Roles of IL-27 in Cancer Biology and Immunotherapy","volume":"2017","author":"Fabbi","year":"2017","journal-title":"Mediat. Inflamm."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s12929-021-00725-7","article-title":"Mesenchymal Stem\/Stromal Cell-Based Therapy: Mechanism, Systemic Safety and Biodistribution for Precision Clinical Applications","volume":"28","author":"Zhuang","year":"2021","journal-title":"J. Biomed. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/j.biocel.2008.01.007","article-title":"Mesenchymal Stem Cells: A Promising Candidate in Regenerative Medicine","volume":"40","author":"Chen","year":"2008","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.cytogfr.2009.10.002","article-title":"Mechanisms Involved in the Therapeutic Properties of Mesenchymal Stem Cells","volume":"20","author":"Fontes","year":"2009","journal-title":"Cytokine Growth Factor Rev."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Linero, I., and Chaparro, O. (2014). Paracrine Effect of Mesenchymal Stem Cells Derived from Human Adipose Tissue in Bone Regeneration. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0107001"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1016\/j.yexcr.2010.05.009","article-title":"Mesenchymal Stem Cells: Paracrine Signaling and Differentiation during Cutaneous Wound Repair","volume":"316","author":"Hocking","year":"2010","journal-title":"Exp. Cell Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.molmed.2010.02.005","article-title":"Mesenchymal Stem Cell Therapy: Two Steps Forward, One Step Back","volume":"16","author":"Ankrum","year":"2010","journal-title":"Trends Mol. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/j.joca.2012.06.002","article-title":"Intra-Articular Injection of Human Mesenchymal Stem Cells (MSCs) Promote Rat Meniscal Regeneration by Being Activated to Express Indian Hedgehog That Enhances Expression of Type II Collagen","volume":"20","author":"Horie","year":"2012","journal-title":"Osteoarthr. Cartil."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"334","DOI":"10.4161\/cam.3.4.9246","article-title":"The Roles of Cell Adhesion Molecules in Tumor Suppression and Cell Migration: A New Paradox","volume":"3","author":"Moh","year":"2009","journal-title":"Cell Adh. Migr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/S0092-8674(00)81279-9","article-title":"Cell Adhesion: The Molecular Basis of Tissue Architecture and Morphogenesis","volume":"84","author":"Gumbiner","year":"1996","journal-title":"Cell"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"109389","DOI":"10.1155\/2014\/109389","article-title":"Conditioned Media from Human Adipose Tissue-Derived Mesenchymal Stem Cells and Umbilical Cord-Derived Mesenchymal Stem Cells Efficiently Induced the Apoptosis and Differentiation in Human Glioma Cell Lines in Vitro","volume":"2014","author":"Yang","year":"2014","journal-title":"Biomed Res. Int."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Velpula, K.K., Dasari, V.R., Tsung, A.J., Gondi, C.S., Klopfenstein, J.D., Mohanam, S., and Rao, J.S. (2011). Regulation of Glioblastoma Progression by Cord Blood Stem Cells Is Mediated by Downregulation of Cyclin D1. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0018017"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"631","DOI":"10.3727\/096368915X687787","article-title":"Paracrine Effects of Mesenchymal Stem Cells Induce Senescence and Differentiation of Glioblastoma Stem-like Cells","volume":"24","author":"Motaln","year":"2015","journal-title":"Cell Transplant."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"241","DOI":"10.4103\/0028-3886.79134","article-title":"Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Inhibit C6 Glioma via Downregulation of Cyclin D1","volume":"59","author":"Jiao","year":"2011","journal-title":"Neurol. India"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1158\/0008-5472.CAN-08-2750","article-title":"Rat Umbilical Cord Stern Cells Completely Abolish Rat Mammary Carcinomas with No Evidence of Metastasis or Recurrence 100 Days Post-Tumor Cell Inoculation","volume":"69","author":"Ganta","year":"2009","journal-title":"Cancer Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.1002\/jcb.24073","article-title":"Human Umbilical Cord Wharton\u2019s Jelly Stem Cell (HWJSC) Extracts Inhibit Cancer Cell Growth in Vitro","volume":"113","author":"Gauthaman","year":"2012","journal-title":"J. Cell. Biochem."},{"key":"ref_61","first-page":"8536","article-title":"Suppression of Tumor Cell Proliferation and Migration by Human Umbilical Cord Mesenchymal Stem Cells: A Possible Role for Apoptosis and Wnt Signaling","volume":"15","author":"Yuan","year":"2018","journal-title":"Oncol. Lett."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1186\/s13048-019-0546-9","article-title":"Anti-Proliferative Effects of Mesenchymal Stem Cells (MSCs) Derived from Multiple Sources on Ovarian Cancer Cell Lines: An in-Vitro Experimental Study","volume":"12","author":"Khalil","year":"2019","journal-title":"J. Ovarian Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"4842134","DOI":"10.1155\/2016\/4842134","article-title":"Wharton\u2019s Jelly-Derived Mesenchymal Stromal Cells and Fibroblast-Derived Extracellular Matrix Synergistically Activate Apoptosis in a P21-Dependent Mechanism in WHCO1 and MDA MB 231 Cancer Cells In Vitro","volume":"2016","author":"Dzobo","year":"2016","journal-title":"Stem Cells Int."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"15078","DOI":"10.1073\/pnas.1318780111","article-title":"KAISO, a Critical Regulator of P53-Mediated Transcription of CDKN1A and Apoptotic Genes","volume":"111","author":"Koh","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Larson, P.S., Schlechter, B.L., King, C.-L., Yang, Q., Glass, C.N., Mack, C., Pistey, R., de las Morenas, A., and Rosenberg, C.L. (2008). CDKN1C\/P57kip2is a Candidate Tumor Suppressor Gene in Human Breast Cancer. BMC Cancer, 8.","DOI":"10.1186\/1471-2407-8-68"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s40364-020-00228-x","article-title":"Roles of IFN-\u03b3 in Tumor Progression and Regression: A Review","volume":"8","author":"Jorgovanovic","year":"2020","journal-title":"Biomark. Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1097\/CEJ.0000000000000405","article-title":"Prognosis and Outcome in CDH1-Mutant Lobular Breast Cancer","volume":"27","author":"Corso","year":"2018","journal-title":"Eur. J. cancer Prev."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"3739","DOI":"10.1172\/JCI81568","article-title":"RB1 Deficiency in Triple-Negative Breast Cancer Induces Mitochondrial Protein Translation","volume":"126","author":"Jones","year":"2016","journal-title":"J. Clin. Investig."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1177\/1758834014530023","article-title":"The PI3K\/AKT\/MTOR Pathway in Breast Cancer: Targets, Trials and Biomarkers","volume":"6","author":"Paplomata","year":"2014","journal-title":"Ther. Adv. Med. Oncol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1023\/A:1006103831990","article-title":"Cyclin D1 in Breast Cancer","volume":"52","author":"Barnes","year":"1998","journal-title":"Breast Cancer Res. Treat."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.3892\/ijo.2016.3680","article-title":"CSF1 Is Involved in Breast Cancer Progression through Inducing Monocyte Differentiation and Homing","volume":"49","author":"Ding","year":"2016","journal-title":"Int. J. Oncol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s10549-012-2289-9","article-title":"Role of Epidermal Growth Factor Receptor in Breast Cancer","volume":"136","author":"Masuda","year":"2012","journal-title":"Breast Cancer Res. Treat."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"2940","DOI":"10.1038\/onc.2009.180","article-title":"Interleukin-6 Induces an Epithelial\u2013Mesenchymal Transition Phenotype in Human Breast Cancer Cells","volume":"28","author":"Sullivan","year":"2009","journal-title":"Oncogene"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.humpath.2006.01.022","article-title":"C-Jun Activation Is Associated with Proliferation and Angiogenesis in Invasive Breast Cancer","volume":"37","author":"Vleugel","year":"2006","journal-title":"Hum. Pathol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1007\/s12282-011-0321-2","article-title":"The Roles of TGF-\u03b2 Signaling in Carcinogenesis and Breast Cancer Metastasis","volume":"19","author":"Imamura","year":"2012","journal-title":"Breast Cancer"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/nrc865","article-title":"Dissemination and Growth of Cancer Cells in Metastatic Sites","volume":"2","author":"Chambers","year":"2002","journal-title":"Nat. Rev. Cancer"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.ceb.2005.08.002","article-title":"Cell Migration in Tumors","volume":"17","author":"Yamaguchi","year":"2005","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1038\/nrc1075","article-title":"Tumour-Cell Invasion and Migration: Diversity and Escape Mechanisms","volume":"3","author":"Friedl","year":"2003","journal-title":"Nat. Rev. Cancer"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev-pathol-020117-043854","article-title":"Epithelial Mesenchymal Transition in Tumor Metastasis","volume":"13","author":"Mittal","year":"2018","journal-title":"Annu. Rev. Pathol."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Roche, J. (2018). The Epithelial-to-Mesenchymal Transition in Cancer. Cancers, 10.","DOI":"10.3390\/cancers10020052"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"5427","DOI":"10.1007\/s13277-015-4361-7","article-title":"Emerging Prognostic Markers Related to Mesenchymal Characteristics of Poorly Differentiated Breast Cancers","volume":"37","author":"Scimeca","year":"2016","journal-title":"Tumor Biol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.2217\/fon.09.94","article-title":"Epithelial-Mesenchymal Transition in Development and Cancer","volume":"5","author":"Micalizzi","year":"2009","journal-title":"Future Oncol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1166\/ch.2013.1004","article-title":"Epithelial-Mesenchymal Transition---A Hallmark of Breast Cancer Metastasis","volume":"1","author":"Wang","year":"2013","journal-title":"Cancer Hallm."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1038\/nrc.2017.118","article-title":"EMT in Cancer","volume":"18","author":"Brabletz","year":"2018","journal-title":"Nat. Rev. Cancer"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.biocel.2017.12.013","article-title":"The Inhibitory Influence of Adipose Tissue-Derived Mesenchymal Stem Cell Environment and Wnt Antagonism on Breast Tumour Cell Lines","volume":"95","author":"Visweswaran","year":"2018","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Liu, J., Han, G., Liu, H., and Qin, C. (2013). Suppression of Cholangiocarcinoma Cell Growth by Human Umbilical Cord Mesenchymal Stem Cells: A Possible Role of Wnt and Akt Signaling. PLoS ONE, 8.","DOI":"10.1371\/annotation\/7fff93fa-8e80-41f3-b35a-e658cb7256a9"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/0955-0674(93)90028-O","article-title":"The Structural and Functional Analysis of Cadherin Calcium-Dependent Cell Adhesion Molecules","volume":"5","author":"Grunwald","year":"1993","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1002\/ijc.10176","article-title":"CDH1 Mutations Are Present in Both Ductal and Lobular Breast Cancer, but Promoter Allelic Variants Show No Detectable Breast Cancer Risk","volume":"98","author":"Lei","year":"2002","journal-title":"Int. J. Cancer"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"6107","DOI":"10.1002\/j.1460-2075.1995.tb00301.x","article-title":"E-Cadherin Is a Tumour\/Invasion Suppressor Gene Mutated in Human Lobular Breast Cancers","volume":"14","author":"Berx","year":"1995","journal-title":"EMBO J."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"5574","DOI":"10.4049\/jimmunol.167.10.5574","article-title":"Regulation of IFN-Gamma Signaling Is Essential for the Cytotoxic Activity of CD8(+) T Cells","volume":"167","author":"Tau","year":"2001","journal-title":"J. Immunol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"21319","DOI":"10.1038\/srep21319","article-title":"Increased Expression of IDO Associates with Poor Postoperative Clinical Outcome of Patients with Gastric Adenocarcinoma","volume":"6","author":"Liu","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"866014","DOI":"10.3389\/fonc.2022.866014","article-title":"IL-6\/JAK\/STAT3 Signaling in Breast Cancer Metastasis: Biology and Treatment","volume":"12","author":"Manore","year":"2022","journal-title":"Front. Oncol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s10549-014-2989-4","article-title":"Hypomethylation of the MMP7 Promoter and Increased Expression of MMP7 Distinguishes the Basal-like Breast Cancer Subtype from Other Triple-Negative Tumors","volume":"146","author":"Sizemore","year":"2014","journal-title":"Breast Cancer Res. Treat."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"9268","DOI":"10.1111\/jcmm.16848","article-title":"Human Placental Mesenchymal Stem\/Stromal Cells (PMSCs) Inhibit Agonist-Induced Platelet Functions Reducing Atherosclerosis and Thrombosis Phenotypes","volume":"25","author":"Basmaeil","year":"2021","journal-title":"J. Cell. Mol. Med."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Basmaeil, Y., Bahattab, E., Al Subayyil, A., Kulayb, H.B., Alrodayyan, M., Abumaree, M., and Khatlani, T. (2021). Decidua Parietalis Mesenchymal Stem\/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro. Cells, 10.","DOI":"10.3390\/cells10123493"},{"key":"ref_96","first-page":"121","article-title":"The Comparison of Impedance-Based Method of Cell Proliferation Monitoring with Commonly Used Metabolic-Based Techniques","volume":"30","author":"Vistejnova","year":"2009","journal-title":"Neuro Endocrinol. Lett."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.toxrep.2020.02.002","article-title":"Real-Time Cell Analysis System in Cytotoxicity Applications: Usefulness and Comparison with Tetrazolium Salt Assays","volume":"7","author":"Ochocka","year":"2020","journal-title":"Toxicol. Rep."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1042\/BSR20140031","article-title":"Using Real-Time Impedance-Based Assays to Monitor the Effects of Fibroblast-Derived Media on the Adhesion, Proliferation, Migration and Invasion of Colon Cancer Cells","volume":"34","author":"Dowling","year":"2014","journal-title":"Biosci. Rep."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1007\/s13770-019-00207-w","article-title":"Cancer Conditioned Medium Modulates Functional and Phenotypic Properties of Human Decidua Parietalis Mesenchymal Stem\/Stromal Cells","volume":"16","author":"Bahattab","year":"2019","journal-title":"Tissue Eng. Regen. Med."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s13770-020-00239-7","article-title":"Preconditioning of Human Decidua Basalis Mesenchymal Stem\/Stromal Cells with Glucose Increased Their Engraftment and Anti-Diabetic Properties","volume":"17","author":"Basmaeil","year":"2020","journal-title":"Tissue Eng. Regen. 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