{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T16:21:27Z","timestamp":1782490887622,"version":"3.54.5"},"reference-count":42,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T00:00:00Z","timestamp":1685491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto Polit\u00e9cnico Nacional","award":["SIP: 20200670"],"award-info":[{"award-number":["SIP: 20200670"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Methotrexate (MTX) is a folic acid analog and has been used to treat a wide variety of malignant and non-malignant diseases. The wide use of these substances has led to the continuous discharge of the parent compound and its metabolites in wastewater. In conventional wastewater treatment plants, the removal or degradation of drugs is not complete. In order to study the MTX degradation by photolysis and photocatalysis processes, two reactors were used with TiO2 as a catalyst and UV-C lamps as a radiation source. H2O2 addition was also studied (absence and 3 mM\/L), and different initial pHs (3.5, 7, and 9.5) were tested to define the best degradation parameters. Results were analyzed by means of ANOVA and the Tukey test. Results show that photolysis in acidic conditions with 3 mM of H2O2 added is the best condition for MTX degradation in these reactors, with a kinetic constant of 0.028 min\u22121. According to the ANOVA test, all considered factors (process, pH, H2O2 addition, and experimentation time) caused statistically significant differences in the MTX degradation results.<\/jats:p>","DOI":"10.3390\/ijms24119595","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T03:06:00Z","timestamp":1685588760000},"page":"9595","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Statistical Analysis of Methotrexate Degradation by UV-C Photolysis and UV-C\/TiO2 Photocatalysis"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3051-4817","authenticated-orcid":false,"given":"Luis A.","family":"Gonz\u00e1lez-Burciaga","sequence":"first","affiliation":[{"name":"CIIDIR-Unidad Durango, Instituto Polit\u00e9cnico Nacional, Calle Sigma 119, Fracc. 20 de Noviembre II, Durango 34220, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5572-1601","authenticated-orcid":false,"given":"Juan C.","family":"Garc\u00eda-Prieto","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n y Desarrollo Tecnol\u00f3gico del Agua, Universidad de Salamanca, Campo Charro s\/n, 37080 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2512-1111","authenticated-orcid":false,"given":"Cynthia M.","family":"N\u00fa\u00f1ez-N\u00fa\u00f1ez","sequence":"additional","affiliation":[{"name":"Ingenier\u00eda en Tecnolog\u00eda Ambiental, Universidad Polit\u00e9cnica de Durango, Carretera Durango-M\u00e9xico km 9.5, Col. Dolores Hidalgo, Durango 34300, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6356-5998","authenticated-orcid":false,"given":"Jos\u00e9 B.","family":"Proal-N\u00e1jera","sequence":"additional","affiliation":[{"name":"CIIDIR-Unidad Durango, Instituto Polit\u00e9cnico Nacional, Calle Sigma 119, Fracc. 20 de Noviembre II, Durango 34220, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"115744","DOI":"10.1016\/j.trac.2019.115744","article-title":"Recent Trends in Disposal and Treatment Technologies of Emerging-Pollutants\u2014A Critical Review","volume":"122","author":"Khan","year":"2020","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1002\/wer.1163","article-title":"Emerging Pollutants in Water Environment: Occurrence, Monitoring, Fate, and Risk Assessment","volume":"91","author":"Tang","year":"2019","journal-title":"Water Environ. Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Vasilachi, I., Asiminicesei, D., Fertu, D., and Gavrilescu, M. (2021). Occurrence and Fate of Emerging Pollutants in Water Environment and Options for Their Removal. Water, 13.","DOI":"10.3390\/w13020181"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.jenvman.2019.02.100","article-title":"Emerging Pollutants in the Urban Water Cycle in Latin America: A Review of the Current Literature","volume":"237","author":"Mora","year":"2019","journal-title":"J. Environ. Manag."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"132822","DOI":"10.1016\/j.chemosphere.2021.132822","article-title":"Pharmaceuticals as Emerging Pollutants: Case Naproxen an Overview","volume":"291","author":"Carmona","year":"2022","journal-title":"Chemosphere"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"126245","DOI":"10.1016\/j.biortech.2021.126245","article-title":"Algal-Based System for Removal of Emerging Pollutants from Wastewater: A Review","volume":"344","author":"Gondi","year":"2022","journal-title":"Bioresour. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.chroma.2014.08.035","article-title":"Photocatalytic Degradation of Selected Anticancer Drugs and Identification of Their Transformation Products in Water by Liquid Chromatography\u2013High Resolution Mass Spectrometry","volume":"1362","author":"Calza","year":"2014","journal-title":"J. Chromatogr. A"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.watres.2014.03.008","article-title":"Monitoring of Methotrexate Chlorination in Water","volume":"57","author":"Roig","year":"2014","journal-title":"Water Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.chemosphere.2015.07.069","article-title":"Removal of the Anti-Cancer Drug Methotrexate from Water by Advanced Oxidation Processes: Aerobic Biodegradation and Toxicity Studies after Treatment","volume":"141","author":"Lutterbeck","year":"2015","journal-title":"Chemosphere"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.watres.2017.01.040","article-title":"The Role of Bicarbonate Anions in Methotrexate Degradation via UV\/TiO2: Mechanisms, Reactivity and Increased Toxicity","volume":"112","author":"Lai","year":"2017","journal-title":"Water Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2492","DOI":"10.1002\/jbm.a.36755","article-title":"Methotrexate Anticancer Drug Delivery to Breast Cancer Cell Lines by Iron Oxide Magnetic Based Nanocarrier","volume":"107","author":"Attari","year":"2019","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Bedoui, Y., Guillot, X., S\u00e9lambarom, J., Guiraud, P., Giry, C., Jaffar-Bandjee, M.C., Ralandison, S., and Gasque, P. (2019). Methotrexate an Old Drug with New Tricks. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20205023"},{"key":"ref_13","first-page":"2060","article-title":"Anticancer Effects of Methotrexate in Combination with A-tocopherol and A-tocopherol Succinate on Triple-negative Breast Cancer","volume":"41","author":"Wei","year":"2019","journal-title":"Oncol. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"137","DOI":"10.4155\/ipk-2016-0022","article-title":"Clinical Pharmacokinetics of Methotrexate in Oncology","volume":"2","author":"Becker","year":"2017","journal-title":"Int. J. Pharmacokinet."},{"key":"ref_15","unstructured":"Consejo de Salubridad General, and Comisi\u00f3n Interinstitucional del Cuadro B\u00e1sico y Cat\u00e1logo de Insumos del Sector Salud (2017). Cuadro B\u00e1sico y Cat\u00e1logo de Medicamentos, Consejo de Salubridad General. [2017th ed.]."},{"key":"ref_16","unstructured":"Direcci\u00f3n de Prestaciones M\u00e9dicas, Unidad de Atenci\u00f3n M\u00e9dica, and Coordinaci\u00f3n de Unidades de Medicas de Alta Especialidad (2019). Cuadro B\u00e1sico de Medicamentos Instituto Mexicano Del Seguro Social, Instituto Mexicano del Seguro Social."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1097\/MPH.0000000000000206","article-title":"Mercaptopurine\/Methotrexate Maintenance Therapy of Childhood Acute Lymphoblastic Leukemia","volume":"36","author":"Schmiegelow","year":"2014","journal-title":"J. Pediatr. Hematol. Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"105","DOI":"10.2147\/TCRM.S154745","article-title":"Update on Subcutaneous Methotrexate for Inflammatory Arthritis and Psoriasis","volume":"14","author":"Vena","year":"2018","journal-title":"Ther. Clin. Risk Manag."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1586\/1744666X.2015.1026894","article-title":"Systemic Methotrexate for the Treatment of Psoriasis","volume":"11","author":"Puig","year":"2015","journal-title":"Expert Rev. Clin. Immunol."},{"key":"ref_20","unstructured":"(2023, March 25). Methotrexate: Dosing and Uses, Medscape.com. Available online: https:\/\/reference.medscape.com\/drug\/trexall-otrexup-methotrexate-343201."},{"key":"ref_21","unstructured":"(2023, March 25). Methotrexate Dosage, Drugs.com. Available online: https:\/\/www.drugs.com\/dosage\/methotrexate.html#Usual_Adult_Dose_for_Psoriasis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"S42","DOI":"10.1016\/j.chemosphere.2014.04.081","article-title":"Aerobic Activated Sludge Transformation of Methotrexate: Identification of Biotransformation Products","volume":"119","author":"Kosjek","year":"2015","journal-title":"Chemosphere"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"57250","DOI":"10.1039\/C4RA09582K","article-title":"Involvement of Process Parameters and Various Modes of Application of TiO2 Nanoparticles in Heterogeneous Photocatalysis of Pharmaceutical Wastes\u2014A Short Review","volume":"4","author":"Sarkar","year":"2014","journal-title":"RSC Adv."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"8948","DOI":"10.1016\/j.ijhydene.2016.11.057","article-title":"A Comparative Study of Tartrazine Degradation Using UV and Solar Fixed Bed Reactors","volume":"42","author":"Chekir","year":"2017","journal-title":"Int. J. Hydrogen Energy"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/s12665-017-6924-y","article-title":"Pharmaceutical Residues in Aquatic Environment and Water Remediation by TiO2 Heterogeneous Photocatalysis: A Review","volume":"76","author":"Lee","year":"2017","journal-title":"Environ. Earth Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"342001","DOI":"10.1088\/1361-6528\/aac6ea","article-title":"Heterogeneous Photocatalysis and Its Potential Applications in Water and Wastewater Treatment: A Review","volume":"29","author":"Ahmed","year":"2018","journal-title":"Nanotechnology"},{"key":"ref_27","unstructured":"Tokode, O., Prabhu, R., Lawton, L.A., and Robertson, P.K.J. (2015). Environmental Photochemistry Part III, Springer."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.egypro.2014.06.068","article-title":"Treatment Heterogeneous Photocatalysis; Factors Influencing the Photocatalytic Degradation by TiO2","volume":"50","author":"Yasmina","year":"2014","journal-title":"Energy Procedia"},{"key":"ref_29","unstructured":"Litter, M.I. (2005). Environmental Photochemistry Part II, Springer."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1080\/10934529.2012.680414","article-title":"A Comparison between Ozonolysis and Sonolysis\/Ozonolysis Treatments for the Degradation of the Cytostatic Drugs Methotrexate and Doxorubicin: Kinetic and Efficiency Approaches","volume":"47","author":"Somensi","year":"2012","journal-title":"J. Environ. Sci. Health Part A"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6060","DOI":"10.1007\/s11356-021-15820-3","article-title":"Photodegradation of Methotrexate in Aqueous Solution: Degradation Kinetics and Identification of Transformation Products","volume":"29","author":"Espinosa","year":"2022","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"8204","DOI":"10.1039\/C9RA00320G","article-title":"Catalytic Ozonation Process Using a MgO Nano-Catalyst to Degrade Methotrexate from Aqueous Solutions and Cytotoxicity Studies in Human Lung Epithelial Cells (A549) after Treatment","volume":"9","author":"Alinejad","year":"2019","journal-title":"RSC Adv."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2658","DOI":"10.1016\/j.cjche.2020.05.033","article-title":"Degradation of Methotrexate by UV\/Peroxymonosulfate: Kinetics, Effect of Operational Parameters and Mechanism","volume":"28","author":"Kanjal","year":"2020","journal-title":"Chin. J. Chem. Eng."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.jhazmat.2006.06.023","article-title":"Color, TOC and AOX Removals from Pulp Mill Effluent by Advanced Oxidation Processes: A Comparative Study","volume":"139","author":"Catalkaya","year":"2007","journal-title":"J. Hazard. Mater."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1021\/mp034006h","article-title":"Molecular Properties of WHO Essential Drugs and Provisional Biopharmaceutical Classification","volume":"1","author":"Kasim","year":"2004","journal-title":"Mol. Pharm."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Jang, J.-H., Jeong, S.-H., and Lee, Y.-B. (2019). Preparation and In Vitro\/In Vivo Characterization of Polymeric Nanoparticles Containing Methotrexate to Improve Lymphatic Delivery. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20133312"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Babyszko, A., Wanag, A., Sad\u0142owski, M., Kusiak-Nejman, E., and Morawski, A.W. (2022). Synthesis and Characterization of SiO2\/TiO2 as Photocatalyst on Methylene Blue Degradation. Catalysts, 12.","DOI":"10.3390\/catal12111372"},{"key":"ref_38","unstructured":"Reguzzoni, G. (2017). Degradation of Cytotoxic Compounds by TiO2-UV Photocatalysis, Politecnico de Milano."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1080\/10643389.2010.507698","article-title":"Advanced Oxidation Processes for Wastewater Treatment: Formation of Hydroxyl Radical and Application","volume":"42","author":"Wang","year":"2012","journal-title":"Crit. Rev. Environ. Sci. Technol."},{"key":"ref_40","unstructured":"Klamerth, N. (2011). Application of a Solar Photo-Fenton for the Treatment of Contaminants in Municipal Wastewater Effluents. [Ph.D. Thesis, University of Almer\u00eda]."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1016\/S0043-1354(98)00448-5","article-title":"A Kinetic Model for H2O2\/UV Process in a Completely Mixed Batch Reactor","volume":"33","author":"Crittenden","year":"1999","journal-title":"Water Res."},{"key":"ref_42","first-page":"119","article-title":"Comparative Efficiencies of Coliform Bacteria Inactivation in Municipal Wastewater by Photolysis (UV) and Photocatalysis (UV\/TiO2\/SiO2). Case: Treatment Wastewater Plant of Salamanca, Spain","volume":"14","year":"2015","journal-title":"Rev. Mex. Ing. Quim."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/11\/9595\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:46:34Z","timestamp":1760125594000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/11\/9595"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,31]]},"references-count":42,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["ijms24119595"],"URL":"https:\/\/doi.org\/10.3390\/ijms24119595","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,31]]}}}