{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:18:32Z","timestamp":1790673512518,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union-NextGenerationEU","award":["CUP B73C22001250006"],"award-info":[{"award-number":["CUP B73C22001250006"]}]},{"name":"European Union-NextGenerationEU","award":["FFC#06\/2020"],"award-info":[{"award-number":["FFC#06\/2020"]}]},{"name":"Italian Cystic Fibrosis Research Foundation","award":["CUP B73C22001250006"],"award-info":[{"award-number":["CUP B73C22001250006"]}]},{"name":"Italian Cystic Fibrosis Research Foundation","award":["FFC#06\/2020"],"award-info":[{"award-number":["FFC#06\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cystic Fibrosis (CF) is an autosomal recessive genetic disease caused by mutations in the CFTR gene, coding for the CFTR chloride channel. About 10% of the CFTR gene mutations are \u201cstop\u201d mutations that generate a premature termination codon (PTC), thus synthesizing a truncated CFTR protein. A way to bypass PTC relies on ribosome readthrough, which is the ribosome\u2019s capacity to skip a PTC, thus generating a full-length protein. \u201cTRIDs\u201d are molecules exerting ribosome readthrough; for some, the mechanism of action is still under debate. We investigate a possible mechanism of action (MOA) by which our recently synthesized TRIDs, namely NV848, NV914, and NV930, could exert their readthrough activity by in silico analysis and in vitro studies. Our results suggest a likely inhibition of FTSJ1, a tryptophan tRNA-specific 2\u2032-O-methyltransferase.<\/jats:p>","DOI":"10.3390\/ijms24119609","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T03:06:00Z","timestamp":1685588760000},"page":"9609","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Investigating the Inhibition of FTSJ1, a Tryptophan tRNA-Specific 2\u2032-O-Methyltransferase by NV TRIDs, as a Mechanism of Readthrough in Nonsense Mutated CFTR"],"prefix":"10.3390","volume":"24","author":[{"given":"Pietro Salvatore","family":"Carollo","sequence":"first","affiliation":[{"name":"Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5059-8686","authenticated-orcid":false,"given":"Marco","family":"Tutone","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9564-3962","authenticated-orcid":false,"given":"Giulia","family":"Culletta","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignazio","family":"Fiduccia","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Corrao","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5885-164X","authenticated-orcid":false,"given":"Ivana","family":"Pibiri","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4760-7179","authenticated-orcid":false,"given":"Aldo","family":"Di Leonardo","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1996-6529","authenticated-orcid":false,"given":"Maria Grazia","family":"Zizzo","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4463-8156","authenticated-orcid":false,"given":"Raffaella","family":"Melfi","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1992-2024","authenticated-orcid":false,"given":"Andrea","family":"Pace","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Maria","family":"Almerico","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1355-8252","authenticated-orcid":false,"given":"Laura","family":"Lentini","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/227561a0","article-title":"Central Dogma of Molecular Biology","volume":"227","author":"Crick","year":"1970","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1111\/brv.12657","article-title":"Deciphering the Molecular Mechanism of Stop Codon Readthrough","volume":"96","author":"Palma","year":"2021","journal-title":"Biol. 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