{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T06:24:43Z","timestamp":1781850283758,"version":"3.54.5"},"reference-count":33,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T00:00:00Z","timestamp":1685664000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the State Assignment of FSRC \u201cCrystallography and Photonics\u201d RAS in part of X-ray studies"},{"name":"the Shared Research Center of the FSRC \u201cCrystallography and Photonics\u201d RAS"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The emission of nanoscale particles from the surfaces of dental implants leads to the cumulative effect of particle complexes in the bone bed and surrounding soft tissues. Aspects of particle migration with the possibility of their involvement in the development of pathological processes of systemic nature remain unexplored. The aim of this work was to study protein production during the interaction of immunocompetent cells with nanoscale metal particles obtained from the surfaces of dental implants in the supernatants. The ability to migrate nanoscale metal particles with possible involvement in the formation of pathological structures, in particular in the formation of gallstones, was also investigated. The following methods were used: microbiological studies, X-ray microtomography, X-ray fluorescence analysis, flow cytometry, electron microscopy, dynamic light scattering, and multiplex immunofluorescence analysis. For the first time, titanium nanoparticles in gallstones were identified by X-ray fluorescence analysis and electron microscopy with elemental mapping. The multiplex analysis method revealed that the physiological response of the immune system cells, in particular neutrophils, to nanosized metal particles significantly reduced TNF-a production both through direct interaction and through double lipopolysaccharide-induced signaling. For the first time, a significant decrease in TNF-a production was demonstrated when supernatants containing nanoscale metal particles were co-cultured with proinflammatory peritoneal exudate obtained from the peritoneum of the C57Bl\/6J inbred mice line for one day.<\/jats:p>","DOI":"10.3390\/ijms24119678","type":"journal-article","created":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T10:08:41Z","timestamp":1685700521000},"page":"9678","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Emission and Migration of Nanoscale Particles during Osseointegration and Disintegration of Dental Implants in the Clinic and Experiment and the Influence on Cytokine Production"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7632-3234","authenticated-orcid":false,"given":"Varvara","family":"Labis","sequence":"first","affiliation":[{"name":"Stomatology Faculty, A.I. Yevdokimov Moscow State University of Medicine and Dentistry, 20, p. 1 Delegatskaya St., 127473 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernest","family":"Bazikyan","sequence":"additional","affiliation":[{"name":"Stomatology Faculty, A.I. Yevdokimov Moscow State University of Medicine and Dentistry, 20, p. 1 Delegatskaya St., 127473 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0846-4670","authenticated-orcid":false,"given":"Svetlana","family":"Sizova","sequence":"additional","affiliation":[{"name":"Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry RAS, 16\/10 Miklukho-Maklaya St., 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4623-4913","authenticated-orcid":false,"given":"Vladimir","family":"Oleinikov","sequence":"additional","affiliation":[{"name":"Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry RAS, 16\/10 Miklukho-Maklaya St., 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey","family":"Trulioff","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cInstitute of Experimental Medicine\u201d, 12 Acad. Pavlov St., 197022 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Serebriakova","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cInstitute of Experimental Medicine\u201d, 12 Acad. Pavlov St., 197022 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Kudryavtsev","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cInstitute of Experimental Medicine\u201d, 12 Acad. Pavlov St., 197022 Saint-Petersburg, Russia"},{"name":"National Medical Research Center of Oncology Named after N.N. Petrov of Ministry of Health of the Russian Federation, 68 Leningradskaya St., Pesochny, 197758 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry","family":"Khmelenin","sequence":"additional","affiliation":[{"name":"Federal Scientific Research Centre \u201cCrystallography and Photonics\u201d Russian Academy of Sciences, 59 Leninskiy Prospekt, 119333 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Zhigalina","sequence":"additional","affiliation":[{"name":"Federal Scientific Research Centre \u201cCrystallography and Photonics\u201d Russian Academy of Sciences, 59 Leninskiy Prospekt, 119333 Moscow, Russia"},{"name":"Department of Machine-Building Technologies, Bauman Moscow State Technical University, 5\/1 2-ya Baumanskaya St., 105005 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7615-6878","authenticated-orcid":false,"given":"Irina","family":"Dyachkova","sequence":"additional","affiliation":[{"name":"Federal Scientific Research Centre \u201cCrystallography and Photonics\u201d Russian Academy of Sciences, 59 Leninskiy Prospekt, 119333 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3701-9517","authenticated-orcid":false,"given":"Denis","family":"Zolotov","sequence":"additional","affiliation":[{"name":"Federal Scientific Research Centre \u201cCrystallography and Photonics\u201d Russian Academy of Sciences, 59 Leninskiy Prospekt, 119333 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Asadchikov","sequence":"additional","affiliation":[{"name":"Federal Scientific Research Centre \u201cCrystallography and Photonics\u201d Russian Academy of Sciences, 59 Leninskiy Prospekt, 119333 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatyana","family":"Mrugova","sequence":"additional","affiliation":[{"name":"Moscow State Clinical Hospital Named after V.V. Veresaev of the Moscow Healthcare Department, 10 Lobnenskaya St., 127644 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandr","family":"Zurochka","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, 106 Pervomaiskaya St., 620049 Ekaterinburg, Russia"},{"name":"Laboratory of Immunobiotechnology of the Russian-Chinese Center for Systemic Pathology of SUSU (NRU), Federal State Autonomous Educational Institution of Higher Education \u201cSouth Ural State University (National Research University)\u201d 76, Lenin prospekt, 454080 Chelyabinsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Khaidukov","sequence":"additional","affiliation":[{"name":"Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry RAS, 16\/10 Miklukho-Maklaya St., 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan G.","family":"Kozlov","sequence":"additional","affiliation":[{"name":"Institute of Professional Education, I.M. Sechenov First Moscow State Medical University, 8-2 Trubetskaya St., 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1111\/cid.12793","article-title":"Bone loss around oral and orthopedic implants: An immunologically based condition","volume":"21","author":"Albrektsson","year":"2019","journal-title":"Clin. Implant Dent. Relat. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1056914","DOI":"10.3389\/fimmu.2022.1056914","article-title":"Osteoimmune regulation underlies oral implant osseointegration and its perturbation","volume":"13","author":"Albrektsson","year":"2023","journal-title":"Front. 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