{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T15:52:50Z","timestamp":1785167570800,"version":"3.55.0"},"reference-count":31,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,6,3]],"date-time":"2023-06-03T00:00:00Z","timestamp":1685750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004569","name":"Ministry of Science and Higher Education","doi-asserted-by":"publisher","award":["KBN 3PO5B15522"],"award-info":[{"award-number":["KBN 3PO5B15522"]}],"id":[{"id":"10.13039\/501100004569","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004569","name":"Ministry of Science and Higher Education","doi-asserted-by":"publisher","award":["N N402 516039"],"award-info":[{"award-number":["N N402 516039"]}],"id":[{"id":"10.13039\/501100004569","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004281","name":"National Science Centre in Poland","doi-asserted-by":"publisher","award":["KBN 3PO5B15522"],"award-info":[{"award-number":["KBN 3PO5B15522"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004281","name":"National Science Centre in Poland","doi-asserted-by":"publisher","award":["N N402 516039"],"award-info":[{"award-number":["N N402 516039"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sarcoidosis (SA) is a granulomatous disorder, which mostly affects the lungs. Its clinical characteristics resemble tuberculosis (TB), but its treatment is different. The etiology of SA is unknown; however, mycobacterial antigens were proposed as environmental factors in its development. Due to previously revealed immunocomplexemia with mycobacterial antigens in the blood of our SA but not TB patients, and in the search for biomarkers for differential diagnosis of the two disorders, we studied the phagocytic activity of monocytes from both patients\u2019 groups with flow cytometry. With the use of this method, we also analyzed the occurrence of receptors for IgG (Fc\u03b3R) and complement components (CR) at the surface of these monocytes, responsible for phagocytosis of immunocomplexes. We revealed a higher phagocytic activity of monocytes in both disorders, but an increased frequency of monocytes with Fc\u03b3RIII (CD16) and decreased with CR1 (CD35) receptor in the blood of SA vs. TB patients. With regard to our other genetic study on Fc\u03b3RIII variants in SA and TB, this may account for the decreased clearance of immunocomplexes and different immune responses in the two diseases. Thus, the presented analysis not only sheds light on the pathomechanisms of SA and TB but may also support their differential diagnosis.<\/jats:p>","DOI":"10.3390\/ijms24119713","type":"journal-article","created":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T04:01:47Z","timestamp":1685937707000},"page":"9713","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparative Analysis of Fc\u03b3 and Complement Receptors Presence on Monocytes in Pulmonary Sarcoidosis and Tuberculosis"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9687-1016","authenticated-orcid":false,"given":"Marlena","family":"Typiak","sequence":"first","affiliation":[{"name":"Department of General and Medical Biochemistry, Faculty of Biology, University of Gdansk, Wita Stwosza 59 St., 80-308 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5287-5210","authenticated-orcid":false,"given":"Piotr","family":"Trzonkowski","sequence":"additional","affiliation":[{"name":"Department of Medical Immunology, Medical University of Gdansk, Debinki 7 St., 80-211 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Skotarczak","sequence":"additional","affiliation":[{"name":"1st Department of Radiology, Medical University of Gdansk, Mariana Smoluchowskiego 17 St., 80-214 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Dubaniewicz","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, Medical University of Gdansk, Mariana Smoluchowskiego 17 St., 80-214 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S240","DOI":"10.1016\/j.ijmyco.2016.09.031","article-title":"Common features of tuberculosis and sarcoidosis","volume":"5","author":"Mortaz","year":"2016","journal-title":"Int. J. Mycobacteriol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1586\/eci.13.8","article-title":"Tuberculosis in the course of sarcoidosis treatment: Is genotyping necessary for personalized therapy?","volume":"9","author":"Dubaniewicz","year":"2013","journal-title":"Expert Rev. Clin. Immunol."},{"key":"ref_3","unstructured":"Statement on sarcoidosis (1999). Joint statement of the american thoracic society (ats), the european respiratory society (ers) and the world association of sarcoidosis and other granulomatous disorders (wasog) adopted by the ats board of directors and by the ers executive committee, February. Am. J. Respir. Crit. Care Med., 160, 736\u2013755."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.ccm.2008.03.011","article-title":"Etiology of sarcoidosis","volume":"29","author":"Chen","year":"2008","journal-title":"Clin. Chest. Med."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Dubaniewicz, A. (2023). Mycobacterial heat shock proteins in sarcoidosis and tuberculosis. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065084"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.autrev.2009.11.015","article-title":"Mycobacterium tuberculosis heat shock proteins and autoimmunity in sarcoidosis","volume":"9","author":"Dubaniewicz","year":"2010","journal-title":"Autoimmun. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.tube.2005.09.004","article-title":"Serum anti-mycobacterial heat shock proteins antibodies in sarcoidosis and tuberculosis","volume":"86","author":"Dubaniewicz","year":"2006","journal-title":"Tuberculosis"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.humimm.2012.10.007","article-title":"Is mycobacterial heat shock protein 16 kda, a marker of the dormant stage of mycobacterium tuberculosis, a sarcoid antigen?","volume":"74","author":"Dubaniewicz","year":"2013","journal-title":"Hum. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3448","DOI":"10.1128\/JCM.01433-06","article-title":"Mycobacterium tuberculosis complex and mycobacterial heat shock proteins in lymph node tissue from patients with pulmonary sarcoidosis","volume":"44","author":"Dubaniewicz","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s10875-006-9011-9","article-title":"Comparative analysis of mycobacterial heat shock proteins-induced apoptosis of peripheral blood mononuclear cells in sarcoidosis and tuberculosis","volume":"26","author":"Dubaniewicz","year":"2006","journal-title":"J. Clin. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1111\/j.1440-1843.2007.01076.x","article-title":"Mycobacterial heat shock protein-induced blood t lymphocytes subsets and cytokine pattern: Comparison of sarcoidosis with tuberculosis and healthy controls","volume":"12","author":"Dubaniewicz","year":"2007","journal-title":"Respirology"},{"key":"ref_12","first-page":"PA1378","article-title":"Monocyte\/neutrophil phagocytic activity ratio in differentiating tuberculosis from sarcoidosis","volume":"54","author":"Dubaniewicz","year":"2019","journal-title":"Eur. Respir. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1016\/j.humimm.2012.05.005","article-title":"Changed phagocytic activity and pattern of fc\u03b3 and complement receptors on blood monocytes in sarcoidosis","volume":"73","author":"Dubaniewicz","year":"2012","journal-title":"Hum. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2003468","DOI":"10.1183\/13993003.03468-2020","article-title":"Monocytes in sarcoidosis are potent tumour necrosis factor producers and predict disease outcome","volume":"58","author":"Lepzien","year":"2021","journal-title":"Eur. Respir. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"J276","DOI":"10.1016\/j.jaut.2009.11.014","article-title":"The complement system in systemic autoimmune disease","volume":"34","author":"Chen","year":"2010","journal-title":"J. Autoimmun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/nri2206","article-title":"Fcgamma receptors as regulators of immune responses","volume":"8","author":"Nimmerjahn","year":"2008","journal-title":"Nat. Rev. Immunol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"624025","DOI":"10.3389\/fcell.2021.624025","article-title":"Complement receptors and their role in leukocyte recruitment and phagocytosis","volume":"9","author":"Vandendriessche","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.humimm.2014.02.011","article-title":"Polymorphism of fcgr3a gene in sarcoidosis","volume":"75","author":"Typiak","year":"2014","journal-title":"Hum. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/5584_2015_193","article-title":"Polymorphism of fcgr2a, fcgr2c, and fcgr3b genes in the pathogenesis of sarcoidosis","volume":"905","author":"Typiak","year":"2016","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Typiak, M., R\u0119kawiecki, B., R\u0119ba\u0142a, K., and Dubaniewicz, A. (2023). Comparative analysis of fcgr gene polymorphism in pulmonary sarcoidosis and tuberculosis. Cells, 12.","DOI":"10.3390\/cells12091221"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1002\/cyto.b.21280","article-title":"Monocytes and macrophages in flow: An escca initiative on advanced analyses of monocyte lineage using flow cytometry","volume":"92","author":"Lambert","year":"2017","journal-title":"Cytom. B Clin. Cytom"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1111\/j.1462-5822.2007.00981.x","article-title":"Macrophage complement receptors and pathogen clearance","volume":"9","author":"Wiesmann","year":"2007","journal-title":"Cell Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.it.2015.04.005","article-title":"Fc\u03b3r dependent mechanisms of cytotoxic, agonistic, and neutralizing antibody activities","volume":"36","author":"Nimmerjahn","year":"2015","journal-title":"Trends Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/s41577-022-00707-2","article-title":"A guide to antigen processing and presentation","volume":"22","author":"Pishesha","year":"2022","journal-title":"Nat. Rev. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1053\/srin.2003.50005","article-title":"Pulmonary granulomatous inflammation: From sarcoidosis to tuberculosis","volume":"18","author":"Perez","year":"2003","journal-title":"Semin. Respir. Infect."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1164\/rccm.201006-0865CI","article-title":"A concise review of pulmonary sarcoidosis","volume":"183","author":"Baughman","year":"2011","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1183\/13993003.00709-2016","article-title":"Can intermediate monocytes predict response to infliximab therapy in sarcoidosis?","volume":"48","author":"Hijdra","year":"2016","journal-title":"Eur. Respir. J."},{"key":"ref_28","first-page":"501","article-title":"Selective depletion of cd14+ cd16+ monocytes by glucocorticoid therapy","volume":"112","author":"Angstwurm","year":"1998","journal-title":"Clin. Exp. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1046\/j.1365-2249.1996.907600.x","article-title":"Generalized immune activation in pulmonary tuberculosis: Co-activation with hiv infection","volume":"103","author":"Vanham","year":"1996","journal-title":"Clin. Exp. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.1365-2249.1994.tb06589.x","article-title":"Altered expression of cd11\/cd18 on the peripheral blood phagocytes of patients with tuberculosis","volume":"97","author":"Yassin","year":"1994","journal-title":"Clin. Exp. Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3128","DOI":"10.4049\/jimmunol.171.6.3128","article-title":"Dynamics of macrophage cell populations during murine pulmonary tuberculosis","volume":"171","author":"Shim","year":"2003","journal-title":"J. Immunol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/11\/9713\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:48:27Z","timestamp":1760125707000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/11\/9713"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,3]]},"references-count":31,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["ijms24119713"],"URL":"https:\/\/doi.org\/10.3390\/ijms24119713","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,3]]}}}