{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T12:40:28Z","timestamp":1793968828737,"version":"4.8.0"},"reference-count":60,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T00:00:00Z","timestamp":1686700800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["E56C18001780001"],"award-info":[{"award-number":["E56C18001780001"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["DGR XI\/3450"],"award-info":[{"award-number":["DGR XI\/3450"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Lombardy Region","award":["E56C18001780001"],"award-info":[{"award-number":["E56C18001780001"]}]},{"name":"Lombardy Region","award":["DGR XI\/3450"],"award-info":[{"award-number":["DGR XI\/3450"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Listeria monocytogenes is a widespread Gram-positive pathogenic bacterium that causes listeriosis, a rather rare but severe foodborne disease. Pregnant women, infants, the elderly, and immunocompromised individuals are considered particularly at risk. L. monocytogenes can contaminate food and food-processing environments. In particular, ready-to-eat (RTE) products are the most common source associated with listeriosis. L. monocytogenes virulence factors include internalin A (InlA), a surface protein known to facilitate bacterial uptake by human intestinal epithelial cells that express the E-cadherin receptor. Previous studies have demonstrated that the presence of premature stop codon (PMSC) mutations naturally occurring in inlA lead to the production of a truncated protein correlated with attenuate virulence. In this study, 849 L. monocytogenes isolates, collected from food, food-processing plants, and clinical cases in Italy, were typed and analyzed for the presence of PMSCs in the inlA gene using Sanger sequencing or whole-genome sequencing (WGS). PMSC mutations were found in 27% of the isolates, predominantly in those belonging to hypovirulent clones (ST9 and ST121). The presence of inlA PMSC mutations in food and environmental isolates was higher than that in clinical isolates. The results reveal the distribution of the virulence potential of L. monocytogenes circulating in Italy and could help to improve risk assessment approaches.<\/jats:p>","DOI":"10.3390\/ijms241210141","type":"journal-article","created":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T01:32:57Z","timestamp":1686792777000},"page":"10141","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Evaluation of the Virulence Potential of Listeria monocytogenes through the Characterization of the Truncated Forms of Internalin A"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5535-105X","authenticated-orcid":false,"given":"Giulia","family":"Magagna","sequence":"first","affiliation":[{"name":"Food Safety Department, Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna (IZSLER), Via A. Bianchi 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6478-2791","authenticated-orcid":false,"given":"Maria","family":"Gori","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"},{"name":"Coordinated Research Centre EpiSoMI, Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5127-5686","authenticated-orcid":false,"given":"Valeria","family":"Russini","sequence":"additional","affiliation":[{"name":"Food Microbiology Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana \u201cM. Aleandri\u201d, Via Appia Nuova, 1411, 00178 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"De Angelis","sequence":"additional","affiliation":[{"name":"Food Microbiology Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana \u201cM. Aleandri\u201d, Via Appia Nuova, 1411, 00178 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Spinelli","sequence":"additional","affiliation":[{"name":"Food Safety Department, Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna (IZSLER), Via A. Bianchi 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4569-5793","authenticated-orcid":false,"given":"Virginia","family":"Filipello","sequence":"additional","affiliation":[{"name":"Food Safety Department, Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna (IZSLER), Via A. Bianchi 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8121-5956","authenticated-orcid":false,"given":"Vito Massimo","family":"Tranquillo","sequence":"additional","affiliation":[{"name":"Programmazione dei Servizi e Controllo di Gestione, Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna (IZSLER), Via A. Bianchi 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3720-6550","authenticated-orcid":false,"given":"Maria Laura","family":"De Marchis","sequence":"additional","affiliation":[{"name":"Food Microbiology Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana \u201cM. Aleandri\u201d, Via Appia Nuova, 1411, 00178 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1663-0889","authenticated-orcid":false,"given":"Teresa","family":"Boss\u00f9","sequence":"additional","affiliation":[{"name":"Food Microbiology Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana \u201cM. Aleandri\u201d, Via Appia Nuova, 1411, 00178 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Fappani","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"},{"name":"Coordinated Research Centre EpiSoMI, Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"},{"name":"Department of Clinical Sciences and Community Health, Universit\u00e0 degli Studi di Milano, 20122 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4119-701X","authenticated-orcid":false,"given":"Elisabetta","family":"Tanzi","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"},{"name":"Coordinated Research Centre EpiSoMI, Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5494-2544","authenticated-orcid":false,"given":"Guido","family":"Finazzi","sequence":"additional","affiliation":[{"name":"Food Safety Department, Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna (IZSLER), Via A. Bianchi 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1128\/CMR.14.3.584-640.2001","article-title":"Listeria Pathogenesis and Molecular Virulence Determinants","volume":"14","author":"Kuhn","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2488","DOI":"10.1038\/s41467-019-10380-0","article-title":"Hypervirulent Listeria monocytogenes Clones\u2019 Adaption to Mammalian Gut Accounts for Their Association with Dairy Products","volume":"10","author":"Maury","year":"2019","journal-title":"Nat. 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