{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T23:49:22Z","timestamp":1789775362140,"version":"4.0.1"},"reference-count":39,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T00:00:00Z","timestamp":1686787200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo\u2014FAPESP","doi-asserted-by":"publisher","award":["2019\/19949-7"],"award-info":[{"award-number":["2019\/19949-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo\u2014FAPESP","doi-asserted-by":"publisher","award":["2014\/07328-4"],"award-info":[{"award-number":["2014\/07328-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["2019\/19949-7"],"award-info":[{"award-number":["2019\/19949-7"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["2014\/07328-4"],"award-info":[{"award-number":["2014\/07328-4"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The functions of annexin A1 (ANXA1), which is expressed on membranes and in cytoplasmic granules, have been fully described. Nonetheless, the role of this protein in protecting against DNA damage in the nucleus is still emerging and requires further investigation. Here, we investigated the involvement of ANXA1 in the DNA damage response in placental cells. Placenta was collected from ANXA1 knockout mice (AnxA1\u2212\/\u2212) and pregnant women with gestational diabetes mellitus (GDM). The placental morphology and ANXA1 expression, which are related to the modulation of cellular response markers in the presence of DNA damage, were analyzed. The total area of AnxA1\u2212\/\u2212 placenta was smaller due to a reduced labyrinth zone, enhanced DNA damage, and impaired base excision repair (BER) enzymes, which resulted in the induction of apoptosis in the labyrinthine and junctional layers. The placentas of pregnant women with GDM showed reduced expression of AnxA1 in the villous compartment, increased DNA damage, apoptosis, and a reduction of enzymes involved in the BER pathway. Our translational data provide valuable insights into the possible involvement of ANXA1 in the response of placental cells to oxidative DNA damage and represent an advancement in investigations into the mechanisms involved in placental biology.<\/jats:p>","DOI":"10.3390\/ijms241210155","type":"journal-article","created":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T02:03:19Z","timestamp":1686794599000},"page":"10155","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The Role of Annexin A1 in DNA Damage Response in Placental Cells: Impact on Gestational Diabetes Mellitus"],"prefix":"10.3390","volume":"24","author":[{"given":"Jusciele Brogin","family":"Moreli","sequence":"first","affiliation":[{"name":"Post-Graduation in Structural and Functional Biology, Federal University of S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04023-062, Brazil"},{"name":"Faceres School of Medicine (FACERES), S\u00e3o Jos\u00e9 do Rio Preto 15090-305, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayk Ricardo dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Biosciences, Humanities and Exact Sciences, S\u00e3o Paulo State University (UNESP), S\u00e3o Jos\u00e9 do Rio Preto 15054-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iracema de Mattos Paranhos","family":"Calderon","sequence":"additional","affiliation":[{"name":"Graduate Program in Gynecology, Obstetrics and Mastology, Botucatu Medical School, S\u00e3o Paulo State University (UNESP), Botucatu 18618-687, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1259-6708","authenticated-orcid":false,"given":"Cristina Bichels","family":"Hebeda","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analyses, Faculty of Pharmaceutical Sciences, University of Sao Paulo (USP), S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra Helena Poliselli","family":"Farsky","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analyses, Faculty of Pharmaceutical Sciences, University of Sao Paulo (USP), S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4178-4625","authenticated-orcid":false,"given":"Estela","family":"Bevilacqua","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of S\u00e3o Paulo (USP), S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0918-2130","authenticated-orcid":false,"given":"Sonia Maria","family":"Oliani","sequence":"additional","affiliation":[{"name":"Post-Graduation in Structural and Functional Biology, Federal University of S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04023-062, Brazil"},{"name":"Department of Biology, School of Biosciences, Humanities and Exact Sciences, S\u00e3o Paulo State University (UNESP), S\u00e3o Jos\u00e9 do Rio Preto 15054-000, Brazil"},{"name":"Advanced Research Center in Medicine (CEPAM), Uni\u00e3o das Faculdades dos Grandes Lagos (Unilago), S\u00e3o Jos\u00e9 do Rio Preto 15030-070, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1111\/j.1476-5381.1988.tb11614.x","article-title":"Lipocortin and the mechanism of action of the glucocorticoids","volume":"94","author":"Flower","year":"1988","journal-title":"Br. 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