{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T05:07:37Z","timestamp":1764133657488,"version":"build-2065373602"},"reference-count":77,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T00:00:00Z","timestamp":1686182400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002809","name":"Generalitat de Catalunya","doi-asserted-by":"publisher","award":["2014SGR789","72150119"],"award-info":[{"award-number":["2014SGR789","72150119"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministerio de Educaci\u00f3n, Comisi\u00f3n Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","award":["2014SGR789","72150119"],"award-info":[{"award-number":["2014SGR789","72150119"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mast cells (MCs) are involved in several immune-related responses, including those in bacterial infections, autoimmune diseases, inflammatory bowel diseases, and cancer, among others. MCs identify microorganisms by pattern recognition receptors (PRRs), activating a secretory response. Interleukin (IL)-10 has been described as an important modulator of MC responses; however, its role in PRR-mediated activation of MC is not fully understood. We analyzed the activation of TLR2, TLR4, TLR7 and Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mucosal-like MCs (MLMCs) and peritoneum-derived cultured MCs (PCMCs) from IL-10\u2212\/\u2212 and wild-type (WT) mice. IL-10\u2212\/\u2212 mice showed a reduced expression of TLR4 and NOD2 at week 6 and TLR7 at week 20 in MLMC. In MLMC and PCMC, TLR2 activation induced a reduced secretion of IL-6 and TNF\u03b1 in IL-10\u2212\/\u2212 MCs. TLR4- and TLR7-mediated secretion of IL-6 and TNF\u03b1 was not detected in PCMCs. Finally, no cytokine release was induced by NOD2 ligand, and responses to TLR2 and TLR4 were lower in MCs at 20 weeks. These findings indicate that PRR activation in MCs depends on the phenotype, ligand, age, and IL-10.<\/jats:p>","DOI":"10.3390\/ijms24129875","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T03:59:56Z","timestamp":1686196796000},"page":"9875","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["IL-10 Modulates the Expression and Activation of Pattern Recognition Receptors in Mast Cells"],"prefix":"10.3390","volume":"24","author":[{"given":"Roberto","family":"Riquelme-Neira","sequence":"first","affiliation":[{"name":"N\u00facleo de Investigaciones Aplicadas en Ciencias Veterinarias y Agron\u00f3micas, Facultad de Medicina Veterinaria y Agronom\u00eda, Universidad de Las Am\u00e9ricas, Sede Concepci\u00f3n, Chacabuco 539, Concepci\u00f3n 4070254, Chile"},{"name":"Department of Cell Biology, Physiology and Immunology, Universitat Aut\u00f2noma de Barcelona, 08193 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romina","family":"Walker-Vergara","sequence":"additional","affiliation":[{"name":"N\u00facleo de Investigaciones Aplicadas en Ciencias Veterinarias y Agron\u00f3micas, Facultad de Medicina Veterinaria y Agronom\u00eda, Universidad de Las Am\u00e9ricas, Sede Concepci\u00f3n, Chacabuco 539, Concepci\u00f3n 4070254, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1788-4928","authenticated-orcid":false,"given":"Joan Antoni","family":"Fern\u00e1ndez-Blanco","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Physiology and Immunology, Universitat Aut\u00f2noma de Barcelona, 08193 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrocinio","family":"Vergara","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Physiology and Immunology, Universitat Aut\u00f2noma de Barcelona, 08193 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,8]]},"reference":[{"key":"ref_1","first-page":"620","article-title":"Mast Cell: A Multi-Functional Master Cell","volume":"6","author":"Dileepan","year":"2016","journal-title":"Front. 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