{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:48:32Z","timestamp":1787028512203,"version":"build-2736575974"},"reference-count":89,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T00:00:00Z","timestamp":1686268800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK 119482"],"award-info":[{"award-number":["DK 119482"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The bladder urothelium releases ATP into the lamina propria (LP) during filling, which can activate P2X receptors on afferent neurons and trigger the micturition reflex. Effective ATP concentrations are largely dependent on metabolism by membrane-bound and soluble ectonucleotidases (s-ENTDs), and the latter are released in the LP in a mechanosensitive manner. Pannexin 1 (PANX1) channel and P2X7 receptor (P2X7R) participate in urothelial ATP release and are physically and functionally coupled, hence we investigated whether they modulate s-ENTDs release. Using ultrasensitive HPLC-FLD, we evaluated the degradation of 1,N6-etheno-ATP (eATP, substrate) to eADP, eAMP, and e-adenosine (e-ADO) in extraluminal solutions that were in contact with the LP of mouse detrusor-free bladders during filling prior to substrate addition, as an indirect measure of s-ENDTS release. Deletion of Panx1 increased the distention-induced, but not the spontaneous, release of s-ENTDs, whereas activation of P2X7R by BzATP or high concentration of ATP in WT bladders increased both. In Panx1\u2212\/\u2212 bladders or WT bladders treated with the PANX1 inhibitory peptide 10Panx, however, BzATP had no effect on s-ENTDS release, suggesting that P2X7R activity depends on PANX1 channel opening. We concluded, therefore, that P2X7R and PANX1 are in complex interaction to regulate s-ENTDs release and maintain suitable ATP concentrations in the LP. Thus, while stretch-activated PANX1 hinders s-ENTDS release possibly to preserve effective ATP concentration at the end of bladder filling, P2X7R activation, presumably in cystitis, would facilitate s-ENTDs-mediated ATP degradation to counteract excessive bladder excitability.<\/jats:p>","DOI":"10.3390\/ijms24129964","type":"journal-article","created":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T09:54:32Z","timestamp":1686304472000},"page":"9964","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Pannexin 1 Channel and the P2X7 Receptor Are in Complex Interplay to Regulate the Release of Soluble Ectonucleotidases in the Murine Bladder Lamina Propria"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5210-9137","authenticated-orcid":false,"given":"Mafalda S. L.","family":"Aresta Branco","sequence":"first","affiliation":[{"name":"Department of Physiology and Cell Biology, School of Medicine, University of Nevada Reno, Reno, NV 89557, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4240-2740","authenticated-orcid":false,"given":"Alejandro","family":"Gutierrez Cruz","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, School of Medicine, University of Nevada Reno, Reno, NV 89557, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren E.","family":"Peri","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, School of Medicine, University of Nevada Reno, Reno, NV 89557, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2519-3323","authenticated-orcid":false,"given":"Violeta N.","family":"Mutafova-Yambolieva","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, School of Medicine, University of Nevada Reno, Reno, NV 89557, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"F332","DOI":"10.1152\/ajprenal.00340.2005","article-title":"Kinetics of Urothelial ATP Release","volume":"291","author":"Lewis","year":"2006","journal-title":"Am. J. Physiol.-Ren. Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1113\/JP276924","article-title":"An Ex Vivo Bladder Model with Detrusor Smooth Muscle Removed to Analyse Biologically Active Mediators Released from the Suburothelium","volume":"597","author":"Durnin","year":"2019","journal-title":"J. Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/j.1469-7793.1997.503bb.x","article-title":"ATP Is Released from Rabbit Urinary Bladder Epithelial Cells by Hydrostatic Pressure Changes-Possible Sensory Mechanism?","volume":"505","author":"Ferguson","year":"1997","journal-title":"J. Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s11302-013-9395-y","article-title":"Purinergic Signalling in the Urinary Tract in Health and Disease","volume":"10","author":"Burnstock","year":"2014","journal-title":"Purinergic Signal."},{"key":"ref_5","first-page":"1185","article-title":"Mechanosensitive Hydrolysis of ATP and ADP in Lamina Propria of the Murine Bladder by Membrane-Bound and Soluble Nucleotidases","volume":"13","author":"Dayton","year":"2022","journal-title":"Front. Physiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1038\/387076a0","article-title":"Neuronal Release of Soluble Nucleotidases and Their Role in Neurotransmitter Inactivation","volume":"387","author":"Todorov","year":"1997","journal-title":"Nature"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s11302-012-9309-4","article-title":"Cellular Function and Molecular Structure of Ecto-Nucleotidases","volume":"8","author":"Zimmermann","year":"2012","journal-title":"Purinergic Signal."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Negoro, H., Urban-Maldonado, M., Liou, L.S., Spray, D.C., Thi, M.M., and Suadicani, S.O. (2014). Pannexin 1 Channels Play Essential Roles in Urothelial Mechanotransduction and Intercellular Signaling. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0106269"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1113\/jphysiol.2014.283119","article-title":"Pannexin 1 Channels Mediate the Release of ATP into the Lumen of the Rat Urinary Bladder","volume":"593","author":"Beckel","year":"2015","journal-title":"J. Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5071","DOI":"10.1038\/sj.emboj.7601378","article-title":"Pannexin-1 Mediates Large Pore Formation and Interleukin-1\u03b2 Release by the ATP-Gated P2X7 Receptor","volume":"25","author":"Pelegrin","year":"2006","journal-title":"EMBO J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"18143","DOI":"10.1074\/jbc.M109.004804","article-title":"The Pannexin 1 Channel Activates the Inflammasome in Neurons and Astrocytes","volume":"284","author":"Silverman","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1007\/s12031-011-9646-8","article-title":"P2X7 Receptor\u2013Pannexin 1 Hemichannel Association: Effect of Extracellular Calcium on Membrane Permeabilization","volume":"46","author":"Poornima","year":"2012","journal-title":"J. Mol. Neurosci."},{"key":"ref_13","first-page":"3776","article-title":"Splice-Variants of the P2X7 Receptor Reveal Differential Agonist-Dependence and Functional Coupling with Pannexin-1","volume":"125","author":"Xu","year":"2012","journal-title":"J. Cell Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2133","DOI":"10.1042\/BCJ20170257","article-title":"P2X7 Receptor Cross-Talk Regulates ATP-Induced Pannexin 1 Internalization","volume":"474","author":"Boyce","year":"2017","journal-title":"Biochem. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4793338","DOI":"10.1155\/2021\/4793338","article-title":"Pannexin1: Role as a Sensor to Injury Is Attenuated in Pretype 2 Corneal Diabetic Epithelium","volume":"2021","author":"Rhodes","year":"2021","journal-title":"Anal. Cell. Pathol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Bravo, D., Zepeda-Morales, K., Maturana, C.J., Retamal, J.S., Hern\u00e1ndez, A., Pelissier, T., Barra, R., S\u00e1ez-Briones, P., Burgos, H., and Constandil, L. (2022). NMDA and P2X7 Receptors Require Pannexin 1 Activation to Initiate and Maintain Nociceptive Signaling in the Spinal Cord of Neuropathic Rats. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23126705"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"193","DOI":"10.4161\/chan.5.3.15765","article-title":"Pannexin Channels Are Not Gap Junction Hemichannels","volume":"5","author":"Sosinsky","year":"2011","journal-title":"Channels"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.febslet.2004.07.009","article-title":"Pannexin Membrane Channels Are Mechanosensitive Conduits for ATP","volume":"572","author":"Bao","year":"2004","journal-title":"FEBS Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"C752","DOI":"10.1152\/ajpcell.00228.2008","article-title":"P2X 7 Receptor-Pannexin1 Complex: Pharmacology and Signaling","volume":"295","author":"Iglesias","year":"2008","journal-title":"Am. J. Physiol.-Cell Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1007\/s00424-012-1077-z","article-title":"Pannexin 1 Forms an Anion-Selective Channel","volume":"463","author":"Ma","year":"2012","journal-title":"Pflugers Arch."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"118","DOI":"10.4161\/chan.27978","article-title":"Pannexin-1 as a Potentiator of Ligand-Gated Receptor Signaling","volume":"8","author":"Isakson","year":"2014","journal-title":"Channels"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.febslet.2005.12.004","article-title":"Activation of Pannexin 1 Channels by ATP through P2Y Receptors and by Cytoplasmic Calcium","volume":"580","author":"Locovei","year":"2006","journal-title":"FEBS Lett."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4747","DOI":"10.1113\/jphysiol.2014.279299","article-title":"Angiotensin II Mobilizes Intracellular Calcium and Activates Pannexin-1 Channels in Rat Carotid Body Type II Cells via AT1 Receptors","volume":"592","author":"Murali","year":"2014","journal-title":"J. Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"80","DOI":"10.3389\/fphys.2014.00080","article-title":"Connexin and Pannexin Hemichannels Are Regulated by Redox Potential","volume":"5","author":"Retamal","year":"2014","journal-title":"Front. Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1126\/science.1126241","article-title":"Ischemia Opens Neuronal Gap Junction Hemichannels","volume":"312","author":"Thompson","year":"2006","journal-title":"Science"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"7655","DOI":"10.1073\/pnas.0601037103","article-title":"Pannexin 1 in Erythrocytes: Function without a Gap","volume":"103","author":"Locovei","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1002\/glia.22338","article-title":"ATP Signaling Is Deficient in Cultured Pannexin1-Null Mouse Astrocytes","volume":"60","author":"Suadicani","year":"2012","journal-title":"Glia"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6940","DOI":"10.1074\/jbc.RA118.006982","article-title":"Constitutive SRC-Mediated Phosphorylation of Pannexin 1 at Tyrosine 198 Occurs at the Plasma Membrane","volume":"294","author":"DeLalio","year":"2019","journal-title":"J. Biol. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1038\/nn.4236","article-title":"Metabotropic NMDA Receptor Signaling Couples Src Family Kinases to Pannexin-1 during Excitotoxicity","volume":"19","author":"Weilinger","year":"2016","journal-title":"Nat. Neurosci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"14324","DOI":"10.1038\/ncomms14324","article-title":"A Quantized Mechanism for Activation of Pannexin Channels","volume":"8","author":"Chiu","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1038\/nature09413","article-title":"Pannexin 1 Channels Mediate \u2018Find-Me\u2019 Signal Release and Membrane Permeability during Apoptosis","volume":"467","author":"Chekeni","year":"2010","journal-title":"Nature"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"C290","DOI":"10.1152\/ajpcell.00302.2017","article-title":"Pannexin1: A Multifunction and Multiconductance and\/or Permeability Membrane Channel","volume":"315","author":"Wang","year":"2018","journal-title":"Am. J. Physiol.-Cell Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e202012754","DOI":"10.1085\/jgp.202012754","article-title":"Structure versus Function: Are New Conformations of Pannexin 1 yet to Be Resolved?","volume":"153","author":"Mim","year":"2021","journal-title":"J. Gen. Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.bbamcr.2016.10.006","article-title":"Pannexin1 as Mediator of Inflammation and Cell Death","volume":"1864","author":"Willebrords","year":"2017","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.bbadis.2017.12.013","article-title":"Protective Effect of Genetic Deletion of Pannexin1 in Experimental Mouse Models of Acute and Chronic Liver Disease","volume":"1864","author":"Willebrords","year":"2018","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_36","first-page":"1111","article-title":"Expression of Connexins and Pannexins in Diseased Human Liver","volume":"21","author":"Leroy","year":"2022","journal-title":"EXCLI J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0074-7696(04)40002-3","article-title":"Cellular Distribution and Functions of P2 Receptor Subtypes in Different Systems","volume":"Volume 240","author":"Burnstock","year":"2004","journal-title":"International Review of Cytology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1126\/science.272.5262.735","article-title":"The Cytolytic P2Z Receptor for Extracellular ATP Identified as a P 2X Receptor (P2X7)","volume":"272","author":"Surprenant","year":"1996","journal-title":"Science"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/j.1469-7793.2001.00025.x","article-title":"Functional Evidence of Distinct ATP Activation Sites at the Human P2X 7 Receptor","volume":"534","author":"Schmalzing","year":"2001","journal-title":"J. Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1111\/j.1476-5381.2009.00233.x","article-title":"Mammalian P2X7 Receptor Pharmacology: Comparison of Recombinant Mouse, Rat and Human P2X7 Receptors","volume":"157","author":"Namovic","year":"2009","journal-title":"Br. J. Pharmacol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"30046","DOI":"10.1074\/jbc.M001010200","article-title":"A Novel Method for Measurement of Submembrane ATP Concentration","volume":"275","author":"Gribble","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2412","DOI":"10.1172\/JCI24086","article-title":"ATP and Purinergic Receptor\u2013Dependent Membrane Traffic in Bladder Umbrella Cells","volume":"115","author":"Wang","year":"2005","journal-title":"J. Clin. Investig."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.bcp.2017.12.021","article-title":"The P2X7 Receptor: A Main Player in Inflammation","volume":"151","author":"Adinolfi","year":"2018","journal-title":"Biochem. Pharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1038\/s41568-018-0037-0","article-title":"Extracellular ATP and P2 Purinergic Signalling in the Tumour Microenvironment","volume":"18","author":"Sarti","year":"2018","journal-title":"Nat. Rev. Cancer"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1111\/j.1476-5381.2011.01535.x","article-title":"The Role of P2X7 Purinergic Receptors in Inflammatory and Nociceptive Changes Accompanying Cyclophosphamide-Induced Haemorrhagic Cystitis in Mice","volume":"165","author":"Martins","year":"2012","journal-title":"Br. J. Pharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"682520","DOI":"10.3389\/fphar.2021.682520","article-title":"P2X7 Receptor Blockade Protects Against Acrolein-Induced Bladder Damage: A Potential New Therapeutic Approach for the Treatment of Bladder Inflammatory Diseases","volume":"12","author":"Taidi","year":"2021","journal-title":"Front. Pharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3659","DOI":"10.1091\/mbc.e05-03-0222","article-title":"A Novel Recombinant Plasma Membrane-Targeted Luciferase Reveals a New Pathway for ATP Secretion","volume":"16","author":"Pellegatti","year":"2005","journal-title":"Mol. Biol. Cell"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"265","DOI":"10.3389\/fnmol.2018.00265","article-title":"New Insights Into Permeation of Large Cations Through ATP-Gated P2X Receptors","volume":"11","author":"Peverini","year":"2018","journal-title":"Front. Mol. Neurosci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.tcb.2018.01.005","article-title":"The Elusive P2X7 Macropore","volume":"28","author":"Schmalzing","year":"2018","journal-title":"Trends Cell Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1124\/jpet.108.146365","article-title":"Pharmacological Characterization of Pannexin-1 Currents Expressed in Mammalian Cells","volume":"328","author":"Ma","year":"2009","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"C250","DOI":"10.1152\/ajpcell.00433.2008","article-title":"A Permeant Regulating Its Permeation Pore: Inhibition of Pannexin 1 Channels by ATP","volume":"296","author":"Qiu","year":"2009","journal-title":"Am. J. Physiol.-Cell Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1042\/BJ20141551","article-title":"ATP Stimulates Pannexin 1 Internalization to Endosomal Compartments","volume":"470","author":"Boyce","year":"2015","journal-title":"Biochem. J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"102458","DOI":"10.1016\/j.ceca.2021.102458","article-title":"Pannexin 1 Plays a Pro-Survival Role by Attenuating P2X7 Receptor-Mediated Ca2+ Influx","volume":"99","author":"Purohit","year":"2021","journal-title":"Cell Calcium"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1038\/s41598-017-00732-5","article-title":"Unique Residues in the ATP Gated Human P2X7 Receptor Define a Novel Allosteric Binding Pocket for the Selective Antagonist AZ10606120","volume":"7","author":"Allsopp","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/nrn2401","article-title":"The Neural Control of Micturition","volume":"9","author":"Fowler","year":"2008","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Aresta Branco, M.S.L., Gutierrez Cruz, A., Borhani Peikani, M., and Mutafova-Yambolieva, V.N. (2023). Sensory Neurons, PIEZO Channels and PAC1 Receptors Regulate the Mechanosensitive Release of Soluble Ectonucleotidases in the Murine Urinary Bladder Lamina Propria. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24087322"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Gutierrez Cruz, A., Aresta Branco, M.S.L., Perrino, B.A., Sanders, K.M., and Mutafova-Yambolieva, V.N. (2022). Urinary ATP Levels Are Controlled by Nucleotidases Released from the Urothelium in a Regulated Manner. Metabolites, 13.","DOI":"10.3390\/metabo13010030"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2152","DOI":"10.1038\/srep02152","article-title":"Pannexin 1 Involvement in Bladder Dysfunction in a Multiple Sclerosis Model","volume":"3","author":"Negoro","year":"2013","journal-title":"Sci. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1096\/fj.201900961RR","article-title":"TRPV4 Receptor as a Functional Sensory Molecule in Bladder Urothelium: Stretch-independent, Tissue-specific Actions and Pathological Implications","volume":"34","author":"Roberts","year":"2020","journal-title":"FASEB J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"12785","DOI":"10.1096\/fj.202000613RRR","article-title":"Mechanical Stretch Activates Piezo1 in Caveolae of Alveolar Type I Cells to Trigger ATP Release and Paracrine Stimulation of Surfactant Secretion from Alveolar Type II Cells","volume":"34","author":"Diem","year":"2020","journal-title":"FASEB J."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1038\/sj.bjp.0703720","article-title":"P2X Receptor Expression in Mouse Urinary Bladder and the Requirement of P2X 1 Receptors for Functional P2X Receptor Responses in the Mouse Urinary Bladder Smooth Muscle","volume":"131","author":"Vial","year":"2000","journal-title":"Br. J. Pharmacol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Svennersten, K., Hall\u00e9n-Grufman, K., de Verdier, P.J., Wiklund, N.P., and Poljakovic, M. (2015). Localization of P2X Receptor Subtypes 2, 3 and 7 in Human Urinary Bladder. BMC Urol., 15.","DOI":"10.1186\/s12894-015-0075-9"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s12026-011-8203-4","article-title":"Transcriptional Control Mechanisms Associated with the Nucleotide Receptor P2X7, a Critical Regulator of Immunologic, Osteogenic, and Neurologic Functions","volume":"50","author":"Lenertz","year":"2011","journal-title":"Immunol. Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"C27","DOI":"10.1152\/ajpcell.00552.2004","article-title":"Augmented Extracellular ATP Signaling in Bladder Urothelial Cells from Patients with Interstitial Cystitis","volume":"290","author":"Sun","year":"2006","journal-title":"Am. J. Physiol.-Cell Physiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1016\/j.juro.2007.05.116","article-title":"Enhanced Adenosine Triphosphate Release From the Urothelium of Patients With Painful Bladder Syndrome: A Possible Pathophysiological Explanation","volume":"178","author":"Kumar","year":"2007","journal-title":"J. Urol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1016\/j.eururo.2009.11.042","article-title":"In Vitro Release of Adenosine Triphosphate from the Urothelium of Human Bladders with Detrusor Overactivity, Both Neurogenic and Idiopathic","volume":"57","author":"Kumar","year":"2010","journal-title":"Eur. Urol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0090-4295(02)01710-7","article-title":"Effect of Doxazosin on Stretch-Activated Adenosine Triphosphate Release in Bladder Urothelial Cells from Patients with Benign Prostatic Hyperplasia","volume":"60","author":"Sun","year":"2002","journal-title":"Urology"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"F423","DOI":"10.1152\/ajprenal.00056.2003","article-title":"Feline Interstitial Cystitis Results in Mechanical Hypersensitivity and Altered ATP Release from Bladder Urothelium","volume":"285","author":"Birder","year":"2003","journal-title":"Am. J. Physiol.-Ren. Physiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.neuint.2010.12.002","article-title":"Overactive and Underactive Bladder Dysfunction Is Reflected by Alterations in Urothelial ATP and NO Release","volume":"58","author":"Munoz","year":"2011","journal-title":"Neurochem. Int."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.neuint.2006.09.002","article-title":"Receptor Activated Bladder and Spinal ATP Release in Neurally Intact and Chronic Spinal Cord Injured Rats","volume":"50","author":"Salas","year":"2007","journal-title":"Neurochem. Int."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1016\/j.neuint.2004.06.001","article-title":"Botulinum Toxin A Inhibits ATP Release from Bladder Urothelium after Chronic Spinal Cord Injury","volume":"45","author":"Khera","year":"2004","journal-title":"Neurochem. Int."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.neuint.2005.04.021","article-title":"Enhanced ATP Release from Rat Bladder Urothelium during Chronic Bladder Inflammation: Effect of Botulinum Toxin A","volume":"47","author":"Smith","year":"2005","journal-title":"Neurochem. Int."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1016\/S1074-7613(01)00229-1","article-title":"Rapid Secretion of Interleukin-1\u03b2 by Microvesicle Shedding","volume":"15","author":"MacKenzie","year":"2001","journal-title":"Immunity"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1111\/j.1469-7793.1999.0335m.x","article-title":"Kinetics of Cell Lysis, Dye Uptake and Permeability Changes in Cells Expressing the Rat P2X 7 Receptor","volume":"519","author":"Virginio","year":"1999","journal-title":"J. Physiol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"10650","DOI":"10.1074\/jbc.M111.332676","article-title":"Transient P2X7 Receptor Activation Triggers Macrophage Death Independent of Toll-like Receptors 2 and 4, Caspase-1, and Pannexin-1 Proteins","volume":"287","author":"Hanley","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1111\/j.1365-2141.2008.07230.x","article-title":"CD39 Is Incorporated into Plasma Microparticles Where It Maintains Functional Properties and Impacts Endothelial Activation","volume":"142","author":"Banz","year":"2008","journal-title":"Br. J. Haematol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"676","DOI":"10.4049\/jimmunol.1003884","article-title":"Cancer Exosomes Express CD39 and CD73, Which Suppress T Cells through Adenosine Production","volume":"187","author":"Clayton","year":"2011","journal-title":"J. Immunol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0005-2736(81)90512-5","article-title":"Exfoliation of Membrane Ecto-Enzymes in the Form of Micro-Vesicles","volume":"645","author":"Trams","year":"1981","journal-title":"Biochim. Et Biophys. Acta (BBA)-Biomembr."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s11302-009-9141-7","article-title":"The P2X7 Receptor\u2013Pannexin Connection to Dye Uptake and IL-1\u03b2 Release","volume":"5","author":"Pelegrin","year":"2009","journal-title":"Purinergic Signal."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s11302-021-09796-5","article-title":"Involvement of P2X7 Receptors in Chronic Pain Disorders","volume":"18","author":"Ren","year":"2022","journal-title":"Purinergic Signal."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Ren, W., Rubini, P., Tang, Y., Engel, T., and Illes, P. (2021). Inherent P2X7 Receptors Regulate Macrophage Functions during Inflammatory Diseases. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23010232"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1016\/j.neuroscience.2003.08.065","article-title":"Differential Co-Localisation of the P2X7 Receptor Subunit with Vesicular Glutamate Transporters VGLUT1 and VGLUT2 in Rat CNS","volume":"123","author":"Atkinson","year":"2004","journal-title":"Neuroscience"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"7143","DOI":"10.1523\/JNEUROSCI.21-18-07143.2001","article-title":"Neuronal P2X 7 Receptors Are Targeted to Presynaptic Terminals in the Central and Peripheral Nervous Systems","volume":"21","author":"Deuchars","year":"2001","journal-title":"J. Neurosci."},{"key":"ref_84","first-page":"e60344","article-title":"A Decentralized (Ex Vivo) Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling","volume":"2019","author":"Durnin","year":"2019","journal-title":"J. Vis. Exp."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.cell.2019.09.017","article-title":"Full-Length P2X7 Structures Reveal How Palmitoylation Prevents Channel Desensitization","volume":"179","author":"McCarthy","year":"2019","journal-title":"Cell"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/0003-2697(84)90352-X","article-title":"High-Pressure Liquid Chromatographic-Fluorometric Detection of Adenosine and Adenine Nucleotides: Application to Endogenous Content and Electrically Induced Release of Adenyl Purines in Guinea Pig Vas Deferens","volume":"137","author":"Levitt","year":"1984","journal-title":"Anal. Biochem."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1006\/abio.2002.5667","article-title":"High-Performance Liquid Chromatographic Technique for Detection of a Fluorescent Analogue of ADP-Ribose in Isolated Blood Vessel Preparations","volume":"305","author":"Bobalova","year":"2002","journal-title":"Anal. Biochem."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"F708","DOI":"10.1152\/ajprenal.00387.2016","article-title":"Urothelial Purine Release during Filling of Murine and Primate Bladders","volume":"311","author":"Durnin","year":"2016","journal-title":"Am. J. Physiol.-Ren. Physiol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1038\/s41592-022-01615-y","article-title":"The EQIPD Framework for Rigor in the Design, Conduct, Analysis and Documentation of Animal Experiments","volume":"19","author":"Vollert","year":"2022","journal-title":"Nat. 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