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However, host immune responses to the vector and\/or the transgene product remain a major hurdle to successful AAV gene transfer. In contrast to antivector adaptive immunity, the initiation of the innate immunity towards rAAV is still poorly understood but is directly dependent on the interaction between the viral vector and innate immune cells. Here, we used a quantitative transcriptomic-based approach to determine the activation of inflammatory and anti-viral pathways after rAAV8-based infection of monocyte-derived dendritic cells (moDCs) obtained from 12 healthy human donors. We have shown that rAAV8 particles are efficiently internalized, but that this uptake does not induce any detectable transcriptomic change in moDCs in contrast to an adenoviral infection, which upregulates anti-viral pathways. These findings suggest an immunologically favorable profile for rAAV8 serotype with regard to in vitro activation of moDC model. Transcriptomic analysis of rAAV-infected innate immune cells is a powerful method to determine the ability of the viral vector to be seen by these sensor cells, which remains of great importance to better understand the immunogenicity of rAAV vectors and to design immune-stealth products.<\/jats:p>","DOI":"10.3390\/ijms241310447","type":"journal-article","created":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T01:49:32Z","timestamp":1687398572000},"page":"10447","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Transcriptomic Analysis Reveals the Inability of Recombinant AAV8 to Activate Human Monocyte-Derived Dendritic Cells"],"prefix":"10.3390","volume":"24","author":[{"given":"Samer","family":"Masri","sequence":"first","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, INSERM, TaRGeT\u2014Translational Research in Gene Therapy, UMR 1089, F-44200 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Carr\u00e9","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, INSERM, TaRGeT\u2014Translational Research in Gene Therapy, UMR 1089, F-44200 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8127-4323","authenticated-orcid":false,"given":"Nicolas","family":"Jaulin","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, INSERM, TaRGeT\u2014Translational Research in Gene Therapy, UMR 1089, F-44200 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2027-022X","authenticated-orcid":false,"given":"C\u00e9line","family":"Vandamme","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, INSERM, TaRGeT\u2014Translational Research in Gene Therapy, UMR 1089, F-44200 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9lia","family":"Couzini\u00e9","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, INSERM, TaRGeT\u2014Translational Research in Gene Therapy, UMR 1089, F-44200 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lien","family":"Guy-Duch\u00e9","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, INSERM, TaRGeT\u2014Translational Research in Gene Therapy, UMR 1089, F-44200 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5706-8178","authenticated-orcid":false,"given":"Jean-Baptiste","family":"Dupont","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, INSERM, TaRGeT\u2014Translational Research in Gene Therapy, UMR 1089, F-44200 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3825-4359","authenticated-orcid":false,"given":"Allwyn","family":"Pereira","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, INSERM, TaRGeT\u2014Translational Research in Gene Therapy, UMR 1089, F-44200 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Charpentier","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, CNRS, INSERM, SFR Sant\u00e9, UMS 3556, UMS016, F-44000 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3594-0353","authenticated-orcid":false,"given":"Laurent","family":"David","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ITUN, F-44000 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1268-7001","authenticated-orcid":false,"given":"Gwladys","family":"Gernoux","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, INSERM, TaRGeT\u2014Translational Research in Gene Therapy, UMR 1089, F-44200 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6853-7013","authenticated-orcid":false,"given":"Micka\u00ebl","family":"Guilbaud","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, INSERM, TaRGeT\u2014Translational Research in Gene Therapy, UMR 1089, F-44200 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oumeya","family":"Adjali","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, INSERM, TaRGeT\u2014Translational Research in Gene Therapy, UMR 1089, F-44200 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2746","DOI":"10.3389\/fmed.2021.809118","article-title":"Gene Therapy Advances: A Meta-Analysis of AAV Usage in Clinical Settings","volume":"8","author":"Au","year":"2022","journal-title":"Front. 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