{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T09:55:13Z","timestamp":1788170113145,"version":"build-2803163510"},"reference-count":84,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T00:00:00Z","timestamp":1687651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["UIDB\/50026\/2020"],"award-info":[{"award-number":["UIDB\/50026\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["UIDP\/50026\/2020"],"award-info":[{"award-number":["UIDP\/50026\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["PTDC\/NEUNMC\/3648\/2014"],"award-info":[{"award-number":["PTDC\/NEUNMC\/3648\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-016818"],"award-info":[{"award-number":["POCI-01-0145-FEDER-016818"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["2021.08121.BD"],"award-info":[{"award-number":["2021.08121.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["SFRH\/BD\/147947\/2019"],"award-info":[{"award-number":["SFRH\/BD\/147947\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["SFRH\/BD\/140624\/2018"],"award-info":[{"award-number":["SFRH\/BD\/140624\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["SFRH\/BPD\/118779\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/118779\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["UMINHO\/BIL-CNCG\/2022\/11"],"award-info":[{"award-number":["UMINHO\/BIL-CNCG\/2022\/11"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["SFRH\/BD\/101298\/2014"],"award-info":[{"award-number":["SFRH\/BD\/101298\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["2020.05109.BD"],"award-info":[{"award-number":["2020.05109.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["CEECIND\/00685\/2020"],"award-info":[{"award-number":["CEECIND\/00685\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["037\/18"],"award-info":[{"award-number":["037\/18"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["LCF\/PR\/HR21\/52410024"],"award-info":[{"award-number":["LCF\/PR\/HR21\/52410024"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000013"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National funds","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000023"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000023"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["UIDB\/50026\/2020"],"award-info":[{"award-number":["UIDB\/50026\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["UIDP\/50026\/2020"],"award-info":[{"award-number":["UIDP\/50026\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["PTDC\/NEUNMC\/3648\/2014"],"award-info":[{"award-number":["PTDC\/NEUNMC\/3648\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-016818"],"award-info":[{"award-number":["POCI-01-0145-FEDER-016818"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["2021.08121.BD"],"award-info":[{"award-number":["2021.08121.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["SFRH\/BD\/147947\/2019"],"award-info":[{"award-number":["SFRH\/BD\/147947\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["SFRH\/BD\/140624\/2018"],"award-info":[{"award-number":["SFRH\/BD\/140624\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["SFRH\/BPD\/118779\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/118779\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["UMINHO\/BIL-CNCG\/2022\/11"],"award-info":[{"award-number":["UMINHO\/BIL-CNCG\/2022\/11"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["SFRH\/BD\/101298\/2014"],"award-info":[{"award-number":["SFRH\/BD\/101298\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["2020.05109.BD"],"award-info":[{"award-number":["2020.05109.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["CEECIND\/00685\/2020"],"award-info":[{"award-number":["CEECIND\/00685\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["037\/18"],"award-info":[{"award-number":["037\/18"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["LCF\/PR\/HR21\/52410024"],"award-info":[{"award-number":["LCF\/PR\/HR21\/52410024"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000013"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"fellowships","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000023"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000023"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["UIDB\/50026\/2020"],"award-info":[{"award-number":["UIDB\/50026\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["UIDP\/50026\/2020"],"award-info":[{"award-number":["UIDP\/50026\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["PTDC\/NEUNMC\/3648\/2014"],"award-info":[{"award-number":["PTDC\/NEUNMC\/3648\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-016818"],"award-info":[{"award-number":["POCI-01-0145-FEDER-016818"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["2021.08121.BD"],"award-info":[{"award-number":["2021.08121.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["SFRH\/BD\/147947\/2019"],"award-info":[{"award-number":["SFRH\/BD\/147947\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["SFRH\/BD\/140624\/2018"],"award-info":[{"award-number":["SFRH\/BD\/140624\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["SFRH\/BPD\/118779\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/118779\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["UMINHO\/BIL-CNCG\/2022\/11"],"award-info":[{"award-number":["UMINHO\/BIL-CNCG\/2022\/11"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["SFRH\/BD\/101298\/2014"],"award-info":[{"award-number":["SFRH\/BD\/101298\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["2020.05109.BD"],"award-info":[{"award-number":["2020.05109.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["CEECIND\/00685\/2020"],"award-info":[{"award-number":["CEECIND\/00685\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["037\/18"],"award-info":[{"award-number":["037\/18"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["LCF\/PR\/HR21\/52410024"],"award-info":[{"award-number":["LCF\/PR\/HR21\/52410024"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000013"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT Scientific Employment Stimulus (CEEC)\u2014Individual Call position","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000023"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000023"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["UIDB\/50026\/2020"],"award-info":[{"award-number":["UIDB\/50026\/2020"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["UIDP\/50026\/2020"],"award-info":[{"award-number":["UIDP\/50026\/2020"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["PTDC\/NEUNMC\/3648\/2014"],"award-info":[{"award-number":["PTDC\/NEUNMC\/3648\/2014"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-016818"],"award-info":[{"award-number":["POCI-01-0145-FEDER-016818"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["2021.08121.BD"],"award-info":[{"award-number":["2021.08121.BD"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["SFRH\/BD\/147947\/2019"],"award-info":[{"award-number":["SFRH\/BD\/147947\/2019"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["SFRH\/BD\/140624\/2018"],"award-info":[{"award-number":["SFRH\/BD\/140624\/2018"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["SFRH\/BPD\/118779\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/118779\/2016"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["UMINHO\/BIL-CNCG\/2022\/11"],"award-info":[{"award-number":["UMINHO\/BIL-CNCG\/2022\/11"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["SFRH\/BD\/101298\/2014"],"award-info":[{"award-number":["SFRH\/BD\/101298\/2014"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["2020.05109.BD"],"award-info":[{"award-number":["2020.05109.BD"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["CEECIND\/00685\/2020"],"award-info":[{"award-number":["CEECIND\/00685\/2020"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["037\/18"],"award-info":[{"award-number":["037\/18"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["LCF\/PR\/HR21\/52410024"],"award-info":[{"award-number":["LCF\/PR\/HR21\/52410024"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000013"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000013"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005032","name":"Bial Foundation","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000023"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000023"]}],"id":[{"id":"10.13039\/501100005032","id-type":"DOI","asserted-by":"publisher"}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["UIDB\/50026\/2020"],"award-info":[{"award-number":["UIDB\/50026\/2020"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["UIDP\/50026\/2020"],"award-info":[{"award-number":["UIDP\/50026\/2020"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["PTDC\/NEUNMC\/3648\/2014"],"award-info":[{"award-number":["PTDC\/NEUNMC\/3648\/2014"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["POCI-01-0145-FEDER-016818"],"award-info":[{"award-number":["POCI-01-0145-FEDER-016818"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["2021.08121.BD"],"award-info":[{"award-number":["2021.08121.BD"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["SFRH\/BD\/147947\/2019"],"award-info":[{"award-number":["SFRH\/BD\/147947\/2019"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["SFRH\/BD\/140624\/2018"],"award-info":[{"award-number":["SFRH\/BD\/140624\/2018"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["SFRH\/BPD\/118779\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/118779\/2016"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["UMINHO\/BIL-CNCG\/2022\/11"],"award-info":[{"award-number":["UMINHO\/BIL-CNCG\/2022\/11"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["SFRH\/BD\/101298\/2014"],"award-info":[{"award-number":["SFRH\/BD\/101298\/2014"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["2020.05109.BD"],"award-info":[{"award-number":["2020.05109.BD"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["CEECIND\/00685\/2020"],"award-info":[{"award-number":["CEECIND\/00685\/2020"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["037\/18"],"award-info":[{"award-number":["037\/18"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["LCF\/PR\/HR21\/52410024"],"award-info":[{"award-number":["LCF\/PR\/HR21\/52410024"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["NORTE-01-0145-FEDER-000013"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000013"]}]},{"name":"\u201cthe la Caixa\u201d Foundation","award":["NORTE-01-0145-FEDER-000023"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000023"]}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["UIDB\/50026\/2020"],"award-info":[{"award-number":["UIDB\/50026\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["UIDP\/50026\/2020"],"award-info":[{"award-number":["UIDP\/50026\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["PTDC\/NEUNMC\/3648\/2014"],"award-info":[{"award-number":["PTDC\/NEUNMC\/3648\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-016818"],"award-info":[{"award-number":["POCI-01-0145-FEDER-016818"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["2021.08121.BD"],"award-info":[{"award-number":["2021.08121.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["SFRH\/BD\/147947\/2019"],"award-info":[{"award-number":["SFRH\/BD\/147947\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["SFRH\/BD\/140624\/2018"],"award-info":[{"award-number":["SFRH\/BD\/140624\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["SFRH\/BPD\/118779\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/118779\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["UMINHO\/BIL-CNCG\/2022\/11"],"award-info":[{"award-number":["UMINHO\/BIL-CNCG\/2022\/11"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["SFRH\/BD\/101298\/2014"],"award-info":[{"award-number":["SFRH\/BD\/101298\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["2020.05109.BD"],"award-info":[{"award-number":["2020.05109.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["CEECIND\/00685\/2020"],"award-info":[{"award-number":["CEECIND\/00685\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["037\/18"],"award-info":[{"award-number":["037\/18"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["LCF\/PR\/HR21\/52410024"],"award-info":[{"award-number":["LCF\/PR\/HR21\/52410024"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000013"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000023"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000023"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"ICVS Scientific Microscopy Platform","award":["UIDB\/50026\/2020"],"award-info":[{"award-number":["UIDB\/50026\/2020"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["UIDP\/50026\/2020"],"award-info":[{"award-number":["UIDP\/50026\/2020"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["PTDC\/NEUNMC\/3648\/2014"],"award-info":[{"award-number":["PTDC\/NEUNMC\/3648\/2014"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["POCI-01-0145-FEDER-016818"],"award-info":[{"award-number":["POCI-01-0145-FEDER-016818"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["2021.08121.BD"],"award-info":[{"award-number":["2021.08121.BD"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["SFRH\/BD\/147947\/2019"],"award-info":[{"award-number":["SFRH\/BD\/147947\/2019"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["SFRH\/BD\/140624\/2018"],"award-info":[{"award-number":["SFRH\/BD\/140624\/2018"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["SFRH\/BPD\/118779\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/118779\/2016"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["UMINHO\/BIL-CNCG\/2022\/11"],"award-info":[{"award-number":["UMINHO\/BIL-CNCG\/2022\/11"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["SFRH\/BD\/101298\/2014"],"award-info":[{"award-number":["SFRH\/BD\/101298\/2014"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["2020.05109.BD"],"award-info":[{"award-number":["2020.05109.BD"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["CEECIND\/00685\/2020"],"award-info":[{"award-number":["CEECIND\/00685\/2020"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["037\/18"],"award-info":[{"award-number":["037\/18"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["LCF\/PR\/HR21\/52410024"],"award-info":[{"award-number":["LCF\/PR\/HR21\/52410024"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["NORTE-01-0145-FEDER-000013"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000013"]}]},{"name":"ICVS Scientific Microscopy Platform","award":["NORTE-01-0145-FEDER-000023"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Spinocerebellar ataxia type 3 (SCA3) is a rare neurodegenerative disease caused by an abnormal polyglutamine expansion within the ataxin-3 protein (ATXN3). This leads to neurodegeneration of specific brain and spinal cord regions, resulting in a progressive loss of motor function. Despite neuronal death, non-neuronal cells, including astrocytes, are also involved in SCA3 pathogenesis. Astrogliosis is a common pathological feature in SCA3 patients and animal models of the disease. However, the contribution of astrocytes to SCA3 is not clearly defined. Inositol 1,4,5-trisphosphate receptor type 2 (IP3R2) is the predominant IP3R in mediating astrocyte somatic calcium signals, and genetically ablation of IP3R2 has been widely used to study astrocyte function. Here, we aimed to investigate the relevance of IP3R2 in the onset and progression of SCA3. For this, we tested whether IP3R2 depletion and the consecutive suppression of global astrocytic calcium signalling would lead to marked changes in the behavioral phenotype of a SCA3 mouse model, the CMVMJD135 transgenic line. This was achieved by crossing IP3R2 null mice with the CMVMJD135 mouse model and performing a longitudinal behavioral characterization of these mice using well-established motor-related function tests. Our results demonstrate that IP3R2 deletion in astrocytes does not modify SCA3 progression.<\/jats:p>","DOI":"10.3390\/ijms241310606","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T01:42:32Z","timestamp":1687743752000},"page":"10606","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Genetic Ablation of Inositol 1,4,5-Trisphosphate Receptor Type 2 (IP3R2) Fails to Modify Disease Progression in a Mouse Model of Spinocerebellar Ataxia Type 3"],"prefix":"10.3390","volume":"24","author":[{"given":"Daniela","family":"Cunha-Garcia","sequence":"first","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Monteiro-Fernandes","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5920-1067","authenticated-orcid":false,"given":"Joana Sofia","family":"Correia","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreia","family":"Neves-Carvalho","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Catarina","family":"Vila\u00e7a-Ferreira","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f3nia","family":"Guerra-Gomes","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Filipe","family":"Viana","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Filipe","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"},{"name":"IPCA-EST-2Ai, Polytechnic Institute of C\u00e1vado and Ave, Applied Artificial Intelligence Laboratory, Campus of IPCA, 4750-810 Barcelos, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4999-5996","authenticated-orcid":false,"given":"Andreia","family":"Teixeira-Castro","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0920-6350","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Maciel","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9680-4027","authenticated-orcid":false,"given":"Sara","family":"Duarte-Silva","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga\/4805-017 Guimar\u00e3es, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1212\/WNL.28.7.703","article-title":"Autosomal dominant system degeneration in Portuguese families of the Azores Islands. A new genetic disorder involving cerebellar, pyramidal, extrapyramidal and spinal cord motor functions","volume":"28","author":"Coutinho","year":"1978","journal-title":"Neurology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1212\/WNL.30.3.319","article-title":"Clinical criteria for diagnosis of Machado-Joseph disease: Report of a non-Azorena Portuguese family","volume":"30","author":"Lima","year":"1980","journal-title":"Neurology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1038\/ng1194-221","article-title":"CAG expansions in a novel gene for Machado-Joseph disease at chromosome 14q32.1","volume":"8","author":"Kawaguchi","year":"1994","journal-title":"Nat. Genet."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1007\/s100380170060","article-title":"The genomic structure and expression of MJD, the Machado-Joseph disease gene","volume":"46","author":"Ichikawa","year":"2001","journal-title":"J. Hum. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1002\/mds.870070302","article-title":"Machado-Joseph disease: An autosomal dominant motor system degeneration","volume":"7","author":"Rosenberg","year":"1992","journal-title":"Mov. Disord."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1002\/ana.410390411","article-title":"Spinocerebellar ataxia 3 and Machado-Joseph disease: Clinical, molecular, and neuropathological features","volume":"39","author":"Stevanin","year":"1996","journal-title":"Ann. Neurol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s12311-008-0013-4","article-title":"SCA3: Neurological features, pathogenesis and animal models","volume":"7","author":"Riess","year":"2008","journal-title":"Cerebellum"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1007\/s00415-011-5992-2","article-title":"Longitudinal study on MRI intensity changes of Machado-Joseph disease: Correlation between MRI findings and neuropathological changes","volume":"258","author":"Horimoto","year":"2011","journal-title":"J. Neurol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1007\/BF00692697","article-title":"The pathology of Machado-Joseph disease","volume":"58","author":"Coutinho","year":"1982","journal-title":"Acta Neuropathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1093\/brain\/awf208","article-title":"Spinocerebellar ataxia type 3 (Machado-Joseph disease): Severe destruction of the lateral reticular nucleus","volume":"125","author":"Schultz","year":"2002","journal-title":"Brain J. Neurol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1007\/s00702-005-0287-3","article-title":"Spinocerebellar ataxias types 2 and 3: Degeneration of the pre-cerebellar nuclei isolates the three phylogenetically defined regions of the cerebellum","volume":"112","author":"Gierga","year":"2005","journal-title":"J. Neural Transm."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.nbd.2014.09.020","article-title":"A new humanized ataxin-3 knock-in mouse model combines the genetic features, pathogenesis of neurons and glia and late disease onset of SCA3\/MJD","volume":"73","author":"Switonski","year":"2015","journal-title":"Neurobiol. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1007\/s12311-011-0340-8","article-title":"Pathoanatomy of cerebellar degeneration in spinocerebellar ataxia type 2 (SCA2) and type 3 (SCA3)","volume":"11","author":"Scherzed","year":"2012","journal-title":"Cerebellum"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1007\/s13311-013-0255-9","article-title":"Chronic treatment with 17-DMAG improves balance and coordination in a new mouse model of Machado-Joseph disease","volume":"11","author":"Amorim","year":"2014","journal-title":"Neurotherapeutics"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1186\/s12974-018-1278-2","article-title":"Inhibition of NF-\u03baB in astrocytes is sufficient to delay neurodegeneration induced by proteotoxicity in neurons","volume":"15","author":"Li","year":"2018","journal-title":"J. Neuroinflamm."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"187","DOI":"10.3988\/jcn.2021.17.2.187","article-title":"Clinical, Imaging, and Laboratory Markers of Premanifest Spinocerebellar Ataxia 1, 2, 3, and 6: A Systematic Review","volume":"17","author":"Kim","year":"2021","journal-title":"J. Clin. Neurol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1007\/s12311-015-0719-z","article-title":"Cytokines in Machado Joseph Disease\/Spinocerebellar Ataxia 3","volume":"15","author":"Saute","year":"2016","journal-title":"Cerebellum"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"334","DOI":"10.2478\/s13380-012-0040-y","article-title":"Homeostatic function of astrocytes: Ca2+ and Na+ signalling","volume":"3","author":"Parpura","year":"2012","journal-title":"Transl. Neurosci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/nrn.2016.159","article-title":"Functional diversity of astrocytes in neural circuit regulation","volume":"18","author":"Rowitch","year":"2017","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3389\/fnsyn.2018.00045","article-title":"Astrocyte\u2013Neuron Networks: A Multilane Highway of Signaling for Homeostatic Brain Function","volume":"10","author":"Mederos","year":"2018","journal-title":"Front. Synaptic Neurosci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1152\/physrev.00042.2016","article-title":"Physiology of Astroglia","volume":"98","author":"Verkhratsky","year":"2018","journal-title":"Physiol. Rev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/S0166-2236(98)01349-6","article-title":"Tripartite synapses: Glia, the unacknowledged partner","volume":"22","author":"Araque","year":"1999","journal-title":"Trends Neurosci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.neuron.2014.02.007","article-title":"Gliotransmitters Travel in Time and Space","volume":"81","author":"Araque","year":"2014","journal-title":"Neuron"},{"key":"ref_24","first-page":"427","article-title":"Functional Roles of Astrocyte Calcium Elevations: From Synapses to Behavior","volume":"11","author":"Sousa","year":"2017","journal-title":"Front. Cell. Neurosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1016\/j.neuron.2008.09.004","article-title":"What Is the Role of Astrocyte Calcium in Neurophysiology?","volume":"59","author":"Agulhon","year":"2008","journal-title":"Neuron"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1096\/fj.01-0380fje","article-title":"Requirement of functional ryanodine receptor type 3 for astrocyte migration","volume":"16","author":"Matyash","year":"2002","journal-title":"FASEB J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.neuron.2016.12.034","article-title":"Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes","volume":"93","author":"Agarwal","year":"2017","journal-title":"Neuron"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1002\/glia.23531","article-title":"Inositol 1,4,5-trisphosphate receptor type 2-independent Ca2+ release from the endoplasmic reticulum in astrocytes","volume":"67","author":"Okubo","year":"2019","journal-title":"Glia"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1111\/j.1471-4159.2007.04825.x","article-title":"IP3 receptor\/Ca2+ channel: From discovery to new signaling concepts","volume":"102","author":"Mikoshiba","year":"2007","journal-title":"J. Neurochem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/0959-4388(94)90089-2","article-title":"Intracellular channels","volume":"4","author":"Furuichi","year":"1994","journal-title":"Curr. Opin. Neurobiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1615\/CritRevNeurobiol.v10.i1.20","article-title":"Inositol 1,4,5-trisphosphate receptors and calcium signaling","volume":"10","author":"Michikawa","year":"1996","journal-title":"Crit. Rev. Neurobiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1002\/(SICI)1096-9861(19990405)406:2<207::AID-CNE6>3.0.CO;2-7","article-title":"Differential cellular expression of isoforms of inositol 1,4,5-triphosphate receptors in neurons and glia in brain","volume":"406","author":"Sharp","year":"1999","journal-title":"J. Comp. Neurol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.neuroscience.2007.09.058","article-title":"Distribution of inositol-1,4,5-trisphosphate receptor isotypes and ryanodine receptor isotypes during maturation of the rat hippocampus","volume":"150","author":"Hertle","year":"2007","journal-title":"Neuroscience"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2164","DOI":"10.1016\/j.bbamcr.2014.03.007","article-title":"Inositol 1,4,5-trisphosphate receptor-isoform diversity in cell death and survival","volume":"1843","author":"Ivanova","year":"2014","journal-title":"Biochim. Biophys. Acta (BBA)-Mol. Cell Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nature10671","article-title":"Excitation-induced ataxin-3 aggregation in neurons from patients with Machado-Joseph disease","volume":"480","author":"Koch","year":"2011","journal-title":"Nature"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1093\/hmg\/dds449","article-title":"Calpain-mediated ataxin-3 cleavage in the molecular pathogenesis of spinocerebellar ataxia type 3 (SCA3)","volume":"22","author":"Weber","year":"2013","journal-title":"Hum. Mol. Genet."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"12713","DOI":"10.1523\/JNEUROSCI.3909-08.2008","article-title":"Deranged calcium signaling and neurodegeneration in spinocerebellar ataxia type 3","volume":"28","author":"Chen","year":"2008","journal-title":"J. Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2602","DOI":"10.1073\/pnas.0409402102","article-title":"Disturbed Ca2+ signaling and apoptosis of medium spiny neurons in Huntington\u2019s disease","volume":"102","author":"Tang","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/S0896-6273(03)00366-0","article-title":"Huntingtin and huntingtin-associated protein 1 influence neuronal calcium signaling mediated by inositol-(1,4,5) triphosphate receptor type 1","volume":"39","author":"Tang","year":"2003","journal-title":"Neuron"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.bbrc.2011.10.096","article-title":"Genetic ablation and chemical inhibition of IP3R1 reduce mutant huntingtin aggregation","volume":"416","author":"Bauer","year":"2011","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"9148","DOI":"10.1523\/JNEUROSCI.0660-09.2009","article-title":"Deranged calcium signaling and neurodegeneration in spinocerebellar ataxia type 2","volume":"29","author":"Liu","year":"2009","journal-title":"J. Neurosci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1161\/01.RES.0000172556.05576.4c","article-title":"Endothelin-1-induced arrhythmogenic Ca2+ signaling is abolished in atrial myocytes of inositol-1,4,5-trisphosphate(IP3)-receptor type 2-deficient mice","volume":"96","author":"Li","year":"2005","journal-title":"Circ. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1126\/science.1184821","article-title":"Hippocampal short- and long-term plasticity are not modulated by astrocyte Ca2+ signaling","volume":"327","author":"Agulhon","year":"2010","journal-title":"Science"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"18155","DOI":"10.1523\/JNEUROSCI.5289-11.2011","article-title":"Astrocyte calcium signaling transforms cholinergic modulation to cortical plasticity in vivo","volume":"31","author":"Takata","year":"2011","journal-title":"J. Neurosci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"E2832","DOI":"10.1073\/pnas.1206557109","article-title":"Nucleus basalis-enabled stimulus-specific plasticity in the visual cortex is mediated by astrocytes","volume":"109","author":"Chen","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1038\/nm.3162","article-title":"Astrocyte-derived ATP modulates depressive-like behaviors","volume":"19","author":"Cao","year":"2013","journal-title":"Nat. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"384","DOI":"10.3389\/fnbeh.2014.00384","article-title":"Astrocyte IP3R2-dependent Ca2+ signaling is not a major modulator of neuronal pathways governing behavior","volume":"8","author":"Petravicz","year":"2014","journal-title":"Front. Behav. Neurosci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1038\/nn.4649","article-title":"Synapse-specific astrocyte gating of amygdala-related behavior","volume":"20","author":"Jamison","year":"2017","journal-title":"Nat. Neurosci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"6557","DOI":"10.1113\/JP274573","article-title":"Rearing-environment-dependent hippocampal local field potential differences in wild-type and inositol trisphosphate receptor type 2 knockout mice","volume":"595","author":"Tanaka","year":"2017","journal-title":"J. Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"379","DOI":"10.3389\/fncel.2018.00379","article-title":"The Role of Astrocytic Calcium Signaling in the Aged Prefrontal Cortex","volume":"12","author":"Viana","year":"2018","journal-title":"Front. Cell. Neurosci."},{"key":"ref_51","first-page":"5673","article-title":"IP3R2 null mice display a normal acquisition of somatic and neurological development milestones","volume":"54","author":"Nascimento","year":"2020","journal-title":"Eur. J. Neurosci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.nbd.2013.05.005","article-title":"Astrocytic Ca2+ waves mediate activation of extrasynaptic NMDA receptors in hippocampal neurons to aggravate brain damage during ischemia","volume":"58","author":"Dong","year":"2013","journal-title":"Neurobiol. Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1172\/JCI89354","article-title":"Astrocytic calcium release mediates peri-infarct depolarizations in a rodent stroke model","volume":"127","author":"Rakers","year":"2017","journal-title":"J. Clin. Investig."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"11612","DOI":"10.1073\/pnas.1300378110","article-title":"Calcium-dependent N-cadherin up-regulation mediates reactive astrogliosis and neuroprotection after brain injury","volume":"110","author":"Kanemaru","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1002\/glia.23300","article-title":"Aberrant astrocyte Ca2+ signals \u201cAxCa signals\u201d exacerbate pathological alterations in an Alexander disease model","volume":"66","author":"Saito","year":"2018","journal-title":"Glia"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.ceca.2015.09.004","article-title":"Disruption of IP3R2-mediated Ca2+ signaling pathway in astrocytes ameliorates neuronal death and brain damage while reducing behavioral deficits after focal ischemic stroke","volume":"58","author":"Li","year":"2015","journal-title":"Cell Calcium"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1093\/hmg\/ddw190","article-title":"Genetic ablation of IP3 receptor 2 increases cytokines and decreases survival of SOD1G93A mice","volume":"25","author":"Staats","year":"2016","journal-title":"Hum. Mol. Genet."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4967","DOI":"10.1523\/JNEUROSCI.5572-07.2008","article-title":"Loss of IP3 receptor-dependent Ca2+ increases in hippocampal astrocytes does not affect baseline CA1 pyramidal neuron synaptic activity","volume":"28","author":"Petravicz","year":"2008","journal-title":"J. Neurosci."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Takata, N., Nagai, T., Ozawa, K., Oe, Y., Mikoshiba, K., and Hirase, H. (2013). Cerebral Blood Flow Modulation by Basal Forebrain or Whisker Stimulation Can Occur Independently of Large Cytosolic Ca2+ Signaling in Astrocytes. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0066525"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/S0028-3908(00)00005-8","article-title":"CNS plasticity and assessment of forelimb sensorimotor outcome in unilateral rat models of stroke, cortical ablation, parkinsonism and spinal cord injury","volume":"39","author":"Schallert","year":"2000","journal-title":"Neuropharmacology"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1038\/nprot.2009.125","article-title":"The adhesive removal test: A sensitive method to assess sensorimotor deficits in mice","volume":"4","author":"Bouet","year":"2009","journal-title":"Nat. Protoc."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/S0006-3495(91)82193-2","article-title":"Imaging of calcium transients in skeletal muscle fibers","volume":"59","author":"Vergara","year":"1991","journal-title":"Biophys. J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1007\/s12079-018-0477-z","article-title":"Role of defective Ca2+ signaling in skeletal muscle weakness: Pharmacological implications","volume":"12","author":"Agrawal","year":"2018","journal-title":"J. Cell Commun. Signal."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.bbrc.2012.10.094","article-title":"Neuronal overexpression of IP3 receptor 2 is detrimental in mutant SOD1 mice","volume":"429","author":"Staats","year":"2012","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1038\/nn.4001","article-title":"Ca2+ signaling in astrocytes from Ip3r2(-\/-) mice in brain slices and during startle responses in vivo","volume":"18","author":"Srinivasan","year":"2015","journal-title":"Nat. Neurosci."},{"key":"ref_66","first-page":"83","article-title":"A new concept for the mechanism of Ca+(+)-regulation of muscle contraction. Implications for physiological and pharmacological approaches to modulate contractile function of myocardium","volume":"86","author":"Brenner","year":"1991","journal-title":"Basic Res. Cardiol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1124\/pr.114.009654","article-title":"The Physiology, Pathology, and Pharmacology of Voltage-Gated Calcium Channels and Their Future Therapeutic Potential","volume":"67","author":"Zamponi","year":"2015","journal-title":"Pharmacol. Rev."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.ceca.2016.02.005","article-title":"Calcium signaling in skeletal muscle development, maintenance and regeneration","volume":"59","author":"Tu","year":"2016","journal-title":"Cell Calcium"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1126\/science.2321027","article-title":"Two-photon laser scanning fluorescence microscopy","volume":"248","author":"Denk","year":"1990","journal-title":"Science"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1085\/jgp.200509475","article-title":"Calcium Release Domains in Mammalian Skeletal Muscle Studied with Two-photon Imaging and Spot Detection Techniques","volume":"127","author":"DiFranco","year":"2006","journal-title":"J. Gen. Physiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.nbd.2010.05.021","article-title":"Motor uncoordination and neuropathology in a transgenic mouse model of Machado-Joseph disease lacking intranuclear inclusions and ataxin-3 cleavage products","volume":"40","author":"Oliveira","year":"2010","journal-title":"Neurobiol. Dis."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Navarrete, M., Perea, G., de Sevilla, D.F., G\u00f3mez-Gonzalo, M., N\u00fa\u00f1ez, A., Mart\u00edn, E.D., and Araque, A. (2012). Astrocytes Mediate In Vivo Cholinergic-Induced Synaptic Plasticity. PLoS Biol., 10.","DOI":"10.1371\/journal.pbio.1001259"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"3248","DOI":"10.1523\/JNEUROSCI.19-08-03248.1999","article-title":"Characterization of progressive motor deficits in mice transgenic for the human Huntington\u2019s disease mutation","volume":"19","author":"Carter","year":"1999","journal-title":"J. Neurosci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/0091-3057(82)90452-X","article-title":"Tactile extinction: Distinguishing between sensorimotor and motor asymmetries in rats with unilateral nigrostriatal damage","volume":"16","author":"Schallert","year":"1982","journal-title":"Pharmacol. Biochem. Behav."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1007\/s003359900551","article-title":"Behavioral and functional analysis of mouse phenotype: SHIRPA, a proposed protocol for comprehensive phenotype assessment","volume":"8","author":"Rogers","year":"1997","journal-title":"Mamm. Genome"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"3221","DOI":"10.1093\/brain\/awv262","article-title":"Serotonergic signalling suppresses ataxin 3 aggregation and neurotoxicity in animal models of Machado-Joseph disease","volume":"138","author":"Jalles","year":"2015","journal-title":"Brain J. Neurol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1007\/s12311-014-0589-9","article-title":"Lithium chloride therapy fails to improve motor function in a transgenic mouse model of Machado-Joseph disease","volume":"13","author":"Oliveira","year":"2014","journal-title":"Cerebellum"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Esteves, S., Duarte-Silva, S., Naia, L., Neves-Carvalho, A., Teixeira-Castro, A., Rego, A.C., Silva-Fernandes, A., and Maciel, P. (2015). Limited Effect of Chronic Valproic Acid Treatment in a Mouse Model of Machado-Joseph Disease. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0141610"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1002\/mds.27292","article-title":"Neuroprotective Effects of Creatine in the CMVMJD135 Mouse Model of Spinocerebellar Ataxia Type 3","volume":"33","author":"Silva","year":"2018","journal-title":"Mov. Disord."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"3626","DOI":"10.1007\/s12035-018-1332-1","article-title":"Preclinical Evidence Supporting Early Initiation of Citalopram Treatment in Machado-Joseph Disease","volume":"56","author":"Esteves","year":"2019","journal-title":"Mol. Neurobiol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.neuroscience.2015.11.030","article-title":"Combined therapy with m-TOR-dependent and -independent autophagy inducers causes neurotoxicity in a mouse model of Machado\u2013Joseph disease","volume":"313","author":"Maciel","year":"2016","journal-title":"Neuroscience"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Correia, J.S., Neves-Carvalho, A., Mendes-Pinheiro, B., Pires, J., Teixeira, F.G., Lima, R., Monteiro, S., Silva, N.A., Soares-Cunha, C., and Serra, S.C. (2021). Preclinical Assessment of Mesenchymal-Stem-Cell-Based Therapies in Spinocerebellar Ataxia Type 3. Biomedicines, 9.","DOI":"10.3390\/biomedicines9121754"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Campos, A.B., Duarte-Silva, S., Fernandes, B., das Neves, S.P., Marques, F., Teixeira-Castro, A., Neves-Carvalho, A., Monteiro-Fernandes, D., Portugal, C.C., and Socodato, R. (2022). Profiling Microglia in a Mouse Model of Machado-Joseph Disease. Biomedicines, 10.","DOI":"10.3390\/biomedicines10020237"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Campos, A.B., Duarte-Silva, S., Fernandes, B., Coimbra, B., Campos, J., Monteiro-Fernandes, D., Teixeira-Castro, A., Ambr\u00f3sio, A.F., and Maciel, P. (2022). Microglial Depletion Has No Impact on Disease Progression in a Mouse Model of Machado\u2013Joseph Disease. Cells, 11.","DOI":"10.3390\/cells11132022"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/13\/10606\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:00:16Z","timestamp":1760126416000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/13\/10606"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,25]]},"references-count":84,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["ijms241310606"],"URL":"https:\/\/doi.org\/10.3390\/ijms241310606","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,25]]}}}