{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T21:44:30Z","timestamp":1774475070525,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T00:00:00Z","timestamp":1687910400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Russian Federation","award":["075-15-2020-803"],"award-info":[{"award-number":["075-15-2020-803"]}]},{"name":"Ministry of Science and Higher Education of the Russian Federation","award":["FNZG-2022-0002"],"award-info":[{"award-number":["FNZG-2022-0002"]}]},{"name":"State Research Funding for FSASI \u201cChumakov FSC R&amp;D IBP RAS\u201d (Institute of Poliomyelitis) from the Ministry of Science and Higher Education of the Russian Federation","award":["075-15-2020-803"],"award-info":[{"award-number":["075-15-2020-803"]}]},{"name":"State Research Funding for FSASI \u201cChumakov FSC R&amp;D IBP RAS\u201d (Institute of Poliomyelitis) from the Ministry of Science and Higher Education of the Russian Federation","award":["FNZG-2022-0002"],"award-info":[{"award-number":["FNZG-2022-0002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Spirocyclic compounds containing heterocyclic moieties represent promising 3D scaffolds for modern drug design. In the search for novel anti-flaviviral agents, we have obtained a series of 3-[N,N-bis(sulfonyl)amino]isoxazolines containing spiro-annulated cyclooctane rings and assessed their antiviral activity against tick-borne encephalitis (TBEV), yellow fever (YFV), and West Nile (WNV) viruses. The structural analogs of spirocyclic compounds with a single sulfonyl group or 1,2-annulated cyclooctane ring were also investigated. Almost all the studied 3-[N,N-bis(sulfonyl)amino]isoxazolines revealed antiviral activity against TBEV and WNV. The most active against TBEV was spiro-isoxazoline derivative containing p-nitrophenyl groups in the sulfonyl part (EC50 2.0 \u00b1 0.5 \u03bcM), while the highest potency against WNV was found for the compounds with lipophilic substituents in sulfonyl moiety, naphtyl being the most favorable one (EC50 1.3 \u00b1 0.5 \u03bcM). In summary, two novel scaffolds of anti-flaviviral agents based on N,N-bis(sulfonyl)amino]isoxazoline were proposed, and the compounds of this type demonstrated activity against TBEV and WNV.<\/jats:p>","DOI":"10.3390\/ijms241310758","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T00:41:45Z","timestamp":1687999305000},"page":"10758","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["3-[N,N-Bis(sulfonyl)amino]isoxazolines with Spiro-Annulated or 1,2-Annulated Cyclooctane Rings Inhibit Reproduction of Tick-Borne Encephalitis, Yellow Fever, and West Nile Viruses"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0294-9581","authenticated-orcid":false,"given":"Kseniya N.","family":"Sedenkova","sequence":"first","affiliation":[{"name":"Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Artem S.","family":"Sazonov","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitry A.","family":"Vasilenko","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristian S.","family":"Andriasov","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina G.","family":"Eremenko","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuri K.","family":"Grishin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7493-8523","authenticated-orcid":false,"given":"Evgeny V.","family":"Khvatov","sequence":"additional","affiliation":[{"name":"FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), Moscow 108819, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9297-9284","authenticated-orcid":false,"given":"Alexander S.","family":"Goryashchenko","sequence":"additional","affiliation":[{"name":"FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), Moscow 108819, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0331-9933","authenticated-orcid":false,"given":"Victoria I.","family":"Uvarova","sequence":"additional","affiliation":[{"name":"FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), Moscow 108819, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0462-2945","authenticated-orcid":false,"given":"Dmitry I.","family":"Osolodkin","sequence":"additional","affiliation":[{"name":"FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), Moscow 108819, Russia"},{"name":"Institute of Translational Medicine and Biotechnology, Sechenov Moscow State Medical University, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aydar A.","family":"Ishmukhametov","sequence":"additional","affiliation":[{"name":"FSASI \u201cChumakov FSC R&D IBP RAS\u201d (Institute of Poliomyelitis), Moscow 108819, Russia"},{"name":"Institute of Translational Medicine and Biotechnology, Sechenov Moscow State Medical University, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6107-2003","authenticated-orcid":false,"given":"Elena B.","family":"Averina","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1021\/acs.jmedchem.0c01473","article-title":"Spirocyclic Scaffolds in Medicinal Chemistry","volume":"64","author":"Hiesinger","year":"2021","journal-title":"J. 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