{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T18:51:55Z","timestamp":1774464715842,"version":"3.50.1"},"reference-count":62,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T00:00:00Z","timestamp":1687910400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["22-15-00236"],"award-info":[{"award-number":["22-15-00236"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Brain ischemia induces slow voltage shifts in the cerebral cortex, including waves of spreading depolarization (SD) and negative ultraslow potentials (NUPs), which are considered as brain injury markers. However, different electrode materials and locations yield variable SD and NUP features. Here, we compared terminal cortical events during isoflurane or sevoflurane euthanasia using intracortical linear iridium electrode arrays and Ag\/AgCl-based electrodes in the rat somatosensory cortex. Inhalation of anesthetics caused respiratory arrest, associated with hyperpolarization and followed by SD and NUP on both Ir and Ag electrodes. Ag-NUPs were bell shaped and waned within half an hour after death. Ir-NUPs were biphasic, with the early fast phase corresponding to Ag-NUP, and the late absent on Ag electrodes, phase of a progressive depolarizing voltage shift reaching \u2212100 mV by two hours after death. In addition, late Ir-NUPs were more ample in the deep layers than at the cortical surface. Thus, intracortical Ag and Ir electrodes reliably assess early manifestations of terminal brain injury including hyperpolarization, SD and the early phase of NUP, while the late, giant amplitude phase of NUP, which is present only on Ir electrodes, is probably related to the sensitivity of Ir electrodes to a yet unidentified factor related to brain death.<\/jats:p>","DOI":"10.3390\/ijms241310769","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T00:41:45Z","timestamp":1687999305000},"page":"10769","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative Study of Terminal Cortical Potentials Using Iridium and Ag\/AgCl Electrodes"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1510-0947","authenticated-orcid":false,"given":"Bulat","family":"Mingazov","sequence":"first","affiliation":[{"name":"Laboratory of Neurobiology, Kazan Federal University, Kazan 420008, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daria","family":"Vinokurova","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology, Kazan Federal University, Kazan 420008, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6175-9796","authenticated-orcid":false,"given":"Andrei","family":"Zakharov","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology, Kazan Federal University, Kazan 420008, Russia"},{"name":"Department of Physiology, Kazan State Medical University, Kazan 420012, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6763-2841","authenticated-orcid":false,"given":"Roustem","family":"Khazipov","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology, Kazan Federal University, Kazan 420008, Russia"},{"name":"Institut de Neurobiologie de la M\u00e9diterran\u00e9e (Inserm U1249), Aix-Marseille Universit\u00e9, 13273 Marseille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s12028-021-01431-w","article-title":"The Critical Role of Spreading Depolarizations in Early Brain Injury: Consensus and Contention","volume":"37","author":"Andrew","year":"2022","journal-title":"Neurocrit. Care"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1152\/physrev.00027.2014","article-title":"Spreading Depression, Spreading Depolarizations, and the Cerebral Vasculature","volume":"95","author":"Ayata","year":"2015","journal-title":"Physiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1038\/nm.2333","article-title":"The role of spreading depression, spreading depolarization and spreading ischemia in neurological disease","volume":"17","author":"Dreier","year":"2011","journal-title":"Nat. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1016\/j.neuron.2015.04.004","article-title":"The stroke-migraine depolarization continuum","volume":"86","author":"Dreier","year":"2015","journal-title":"Neuron"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"837650","DOI":"10.3389\/fncel.2022.837650","article-title":"Migraine Aura, Transient Ischemic Attacks, Stroke, and Dying of the Brain Share the Same Key Pathophysiological Process in Neurons Driven by Gibbs-Donnan Forces, Namely Spreading Depolarization","volume":"16","author":"Lemale","year":"2022","journal-title":"Front. Cell. Neurosci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1171836","DOI":"10.3389\/fncel.2023.1171836","article-title":"Common and distinct mechanisms of migraine and stroke","volume":"17","author":"Giniatullin","year":"2023","journal-title":"Front. Cell. Neurosci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1091987","DOI":"10.3389\/fneur.2022.1091987","article-title":"Less-invasive subdural electrocorticography for investigation of spreading depolarizations in patients with subarachnoid hemorrhage","volume":"13","author":"Meinert","year":"2023","journal-title":"Front. Neurol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"fcad080","DOI":"10.1093\/braincomms\/fcad080","article-title":"Spreading depolarization and angiographic spasm are separate mediators of delayed infarcts","volume":"5","author":"Horst","year":"2023","journal-title":"Brain Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1152\/jn.1947.10.6.409","article-title":"Further Observations on the Spreading Depression of Activity in the Cerebral Cortex","volume":"10","author":"Leao","year":"1947","journal-title":"J. Neurophysiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/0013-4694(51)90079-X","article-title":"The slow voltage variation of cortical spreading depression of activity","volume":"3","author":"LEAO","year":"1951","journal-title":"Electroencephalogr. Clin. Neurophysiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2738","DOI":"10.1161\/01.STR.0000043073.69602.09","article-title":"Spreading and synchronous depressions of cortical activity in acutely injured human brain","volume":"33","author":"Strong","year":"2002","journal-title":"Stroke"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1152\/physrev.2001.81.3.1065","article-title":"Mechanisms of spreading depression and hypoxic spreading depression-like depolarization","volume":"81","author":"Somjen","year":"2001","journal-title":"Physiol. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1177\/0271678X16654496","article-title":"Recording, analysis, and interpretation of spreading depolarizations in neurointensive care: Review and recommendations of the COSBID research group","volume":"37","author":"Dreier","year":"2017","journal-title":"J. Cereb. Blood Flow. Metab."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1093\/brain\/awab457","article-title":"Spreading depolarizations in ischaemia after subarachnoid haemorrhage, a diagnostic phase III study","volume":"145","author":"Dreier","year":"2022","journal-title":"Brain"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1177\/0271678X231153745","article-title":"Spreading depolarization causes reperfusion failure after cerebral ischemia","volume":"43","author":"Torteli","year":"2023","journal-title":"J. Cereb. Blood Flow. Metab."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Alsbrook, D.L., Di Napoli, M., Bhatia, K., Desai, M., Hinduja, A., Rubinos, C.A., Mansueto, G., Singh, P., Domeniconi, G.G., and Ikram, A. (2023). Pathophysiology of Early Brain Injury and Its Association with Delayed Cerebral Ischemia in Aneurysmal Subarachnoid Hemorrhage: A Review of Current Literature. J. Clin. Med., 12.","DOI":"10.3390\/jcm12031015"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1177\/1073858412453340","article-title":"Is spreading depolarization characterized by an abrupt, massive release of gibbs free energy from the human brain cortex?","volume":"19","author":"Dreier","year":"2013","journal-title":"Neuroscientist"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1734","DOI":"10.1093\/brain\/awy102","article-title":"The negative ultraslow potential, electrophysiological correlate of infarction in the human cortex","volume":"141","author":"Luckl","year":"2018","journal-title":"Brain"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"373","DOI":"10.3389\/fnins.2019.00373","article-title":"Correlates of Spreading Depolarization, Spreading Depression, and Negative Ultraslow Potential in Epidural Versus Subdural Electrocorticography","volume":"13","author":"Dreier","year":"2019","journal-title":"Front. Neurosci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.3171\/2018.7.JNS181478","article-title":"Terminal spreading depolarizations causing electrocortical silencing prior to clinical brain death: Case report","volume":"131","author":"Carlson","year":"2019","journal-title":"J. Neurosurg."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1944","DOI":"10.1177\/0271678X221107422","article-title":"Depth-profile of impairments in endothelin-1\u2014Induced focal cortical ischemia","volume":"42","author":"Vinokurova","year":"2022","journal-title":"J. Cereb. Blood Flow. Metab."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"ENEURO.0246-21.2021","DOI":"10.1523\/ENEURO.0246-21.2021","article-title":"Full-Band EEG Recordings Using Hybrid AC\/DC-Divider Filters","volume":"8","author":"Nasretdinov","year":"2021","journal-title":"eNeuro"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"408","DOI":"10.3389\/fncel.2017.00408","article-title":"Direct Current Coupled Recordings of Cortical Spreading Depression Using Silicone Probes","volume":"11","author":"Nasretdinov","year":"2017","journal-title":"Front. Cell. Neurosci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1111\/j.1399-6576.1995.tb04176.x","article-title":"Propofol and isoflurane induced EEG burst suppression patterns in rabbits","volume":"39","author":"Hartikainen","year":"1995","journal-title":"Acta Anaesthesiol. Scand."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"9850","DOI":"10.1523\/JNEUROSCI.5176-08.2009","article-title":"Cortical inhibition during burst suppression induced with isoflurane anesthesia","volume":"29","author":"Ferron","year":"2009","journal-title":"J. Neurosci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/acn3.16","article-title":"Isoflurane suppresses early cortical activity","volume":"1","author":"Sitdikova","year":"2014","journal-title":"Ann. Clin. Transl. Neurol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1111\/epi.16060","article-title":"Impaired vocal communication, sleep-related discharges, and transient alteration of slow-wave sleep in developing mice lacking the GluN2A subunit of N-methyl-d-aspartate receptors","volume":"60","author":"Salmi","year":"2019","journal-title":"Epilepsia"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"390","DOI":"10.3389\/fncel.2017.00390","article-title":"Preferential Initiation and Spread of Anoxic Depolarization in Layer 4 of Rat Barrel Cortex","volume":"11","author":"Juzekaeva","year":"2017","journal-title":"Front. Cell. Neurosci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"7189","DOI":"10.1523\/JNEUROSCI.18-18-07189.1998","article-title":"Imaging spreading depression and associated intracellular calcium waves in brain slices","volume":"18","author":"Basarsky","year":"1998","journal-title":"J. Neurosci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1152\/jn.2001.85.1.414","article-title":"Imaging anoxic depolarization during ischemia-like conditions in the mouse hemi-brain slice","volume":"85","author":"Joshi","year":"2001","journal-title":"J. Neurophysiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1177\/0271678X16659496","article-title":"Heterogeneous incidence and propagation of spreading depolarizations","volume":"37","author":"Kaufmann","year":"2017","journal-title":"J. Cereb. Blood Flow. Metab."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4733","DOI":"10.1523\/JNEUROSCI.3694-15.2016","article-title":"Susceptibility of Primary Sensory Cortex to Spreading Depolarizations","volume":"36","author":"Bogdanov","year":"2016","journal-title":"J. Neurosci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0304-3940(93)90484-3","article-title":"Spreading depression can be restricted to distinct depths of the rat cerebral cortex","volume":"152","author":"Richter","year":"1993","journal-title":"Neurosci. Lett."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1002\/ana.25147","article-title":"Terminal spreading depolarization and electrical silence in death of human cerebral cortex","volume":"83","author":"Dreier","year":"2018","journal-title":"Ann. Neurol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"10597","DOI":"10.1523\/JNEUROSCI.3440-07.2007","article-title":"Hypersensitivity of the anesthesia-induced comatose brain","volume":"27","author":"Kroeger","year":"2007","journal-title":"J. Neurosci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1002\/1531-8249(200010)48:4<632::AID-ANA10>3.0.CO;2-3","article-title":"Seizures accelerate anoxia-induced neuronal death in the neonatal rat hippocampus","volume":"48","author":"Dzhala","year":"2000","journal-title":"Ann. Neurol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"13510","DOI":"10.1523\/JNEUROSCI.1439-12.2012","article-title":"Optogenetic analysis of neuronal excitability during global ischemia reveals selective deficits in sensory processing following reperfusion in mouse cortex","volume":"32","author":"Chen","year":"2012","journal-title":"J. Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/0006-8993(89)90258-8","article-title":"Adenosine antagonists delay hypoxia-induced depression of neuronal activity in hippocampal brain slice","volume":"490","author":"Fowler","year":"1989","journal-title":"Brain Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0304-3940(93)90814-2","article-title":"Adenosine antagonists prevent hypoxia-induced depression of excitatory but not inhibitory synaptic currents","volume":"159","author":"Katchman","year":"1993","journal-title":"Neurosci. Lett."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2138","DOI":"10.1152\/jn.1995.74.5.2138","article-title":"Hippocampal CA1 lacunosum-moleculare interneurons: Comparison of effects of anoxia on excitatory and inhibitory postsynaptic currents","volume":"74","author":"Khazipov","year":"1995","journal-title":"J. Neurophysiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1002\/1531-8249(199907)46:1<95::AID-ANA14>3.0.CO;2-1","article-title":"Epileptogenic action of caffeine during anoxia in the neonatal rat hippocampus","volume":"46","author":"Dzhala","year":"1999","journal-title":"Ann. Neurol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2251","DOI":"10.1152\/jn.1993.70.6.2251","article-title":"Hippocampal inhibitory interneurons are functionally disconnected from excitatory inputs by anoxia","volume":"70","author":"Khazipov","year":"1993","journal-title":"J. Neurophysiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1038\/333662a0","article-title":"Volatile general anaesthetics activate a novel neuronal K+ current","volume":"333","author":"Franks","year":"1988","journal-title":"Nature"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1038\/8084","article-title":"Inhalational anesthetics activate two-pore-domain background K+ channels","volume":"2","author":"Patel","year":"1999","journal-title":"Nat. Neurosci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1097\/ALN.0b013e31817f5baf","article-title":"Neural mechanisms of sevoflurane-induced respiratory depression in newborn rats","volume":"109","author":"Kuribayashi","year":"2008","journal-title":"Anesthesiology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1177\/0333102412470216","article-title":"Pearls and pitfalls in experimental models of spreading depression","volume":"33","author":"Ayata","year":"2013","journal-title":"Cephalalgia"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"101733","DOI":"10.1016\/j.pneurobio.2019.101733","article-title":"Identifying neuronal correlates of dying and resuscitation in a model of reversible brain anoxia","volume":"185","author":"Schramm","year":"2020","journal-title":"Prog. Neurobiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1152\/physrev.1999.79.4.1431","article-title":"Ischemic cell death in brain neurons","volume":"79","author":"Lipton","year":"1999","journal-title":"Physiol. Rev."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2386","DOI":"10.1111\/epi.16390","article-title":"Segregation of seizures and spreading depolarization across cortical layers","volume":"60","author":"Zakharov","year":"2019","journal-title":"Epilepsia"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1152\/jn.1944.7.6.359","article-title":"Spreading depression of activity in the cerebral cortex","volume":"7","author":"Leao","year":"1944","journal-title":"J. Neurophysiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1106268","DOI":"10.3389\/fncel.2023.1106268","article-title":"Anoxic spreading depolarization in the neonatal rat cortex in vitro","volume":"17","author":"Gainutdinov","year":"2023","journal-title":"Front. Cell. Neurosci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"18970","DOI":"10.1038\/s41598-020-75975-w","article-title":"Reappraisal of anoxic spreading depolarization as a terminal event during oxygen-glucose deprivation in brain slices in vitro","volume":"10","author":"Juzekaeva","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.1523\/JNEUROSCI.5128-07.2008","article-title":"Two-photon imaging of stroke onset in vivo reveals that NMDA-receptor independent ischemic depolarization is the major cause of rapid reversible damage to dendrites and spines","volume":"28","author":"Murphy","year":"2008","journal-title":"J. Neurosci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1007\/s12028-021-01358-2","article-title":"Oxygen-Induced and pH-Induced Direct Current Artifacts on Invasive Platinum\/Iridium Electrodes for Electrocorticography","volume":"35","author":"Major","year":"2021","journal-title":"Neurocrit. Care"},{"key":"ref_55","first-page":"1","article-title":"Negative ultraslow potentials during kidney ischemia","volume":"52","author":"Vinokurova","year":"2022","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1038\/jcbfm.1984.3","article-title":"Extracellular pH changes during spreading depression and cerebral ischemia: Mechanisms of brain pH regulation","volume":"4","author":"Mutch","year":"1984","journal-title":"J. Cereb. Blood Flow. Metab."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1146\/annurev.bioeng.10.061807.160518","article-title":"Neural stimulation and recording electrodes","volume":"10","author":"Cogan","year":"2008","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"ref_58","first-page":"585","article-title":"Fixed negative charge and the Donnan effect: A description of the driving forces associated with brain tissue swelling and oedema","volume":"368","author":"Elkin","year":"2010","journal-title":"Philos. Trans. A Math. Phys. Eng. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1177\/0271678X15617172","article-title":"Molecular pathophysiology of cerebral edema","volume":"36","author":"Stokum","year":"2016","journal-title":"J. Cereb. Blood Flow. Metab."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"502","DOI":"10.3389\/fncel.2018.00502","article-title":"Dynamics of the Hypoxia-Induced Tissue Edema in the Rat Barrel Cortex in vitro","volume":"12","author":"Juzekaeva","year":"2018","journal-title":"Front. Cell. Neurosci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4748","DOI":"10.1073\/pnas.95.8.4748","article-title":"Cardiac arrest in rodents: Maximal duration compatible with a recovery of neuronal activity","volume":"95","author":"Charpak","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1063\/1.555839","article-title":"Standard Electrode-Potentials and Temperature Coefficients in Water at 298.15-K","volume":"18","author":"Bratsch","year":"1989","journal-title":"J. Phys. Chem. Ref. Data"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/13\/10769\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:02:34Z","timestamp":1760126554000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/13\/10769"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,28]]},"references-count":62,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["ijms241310769"],"URL":"https:\/\/doi.org\/10.3390\/ijms241310769","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,28]]}}}