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Nitrogen availability could modulate secondary metabolic pathways as evidenced by the significant differential expression of several core genes involved in mycotoxin biosynthesis and genes encoding polyketide synthase\/nonribosomal peptide synthetases, cytochrome P450 and carbohydrate-active enzymes in Fusarium sacchari, grown on different nitrogen sources. A combined analysis was carried out on the transcript and metabolite profiles of regulatory metabolic processes and the virulence of Fusarium sacchari grown on various nitrogen sources. The nitrogen regulation of the gibberellin gene cluster included the metabolic flux and multiple steps of gibberellin synthesis. UHPLC-MS\/MS-based metabolome analysis revealed the coordination of these related transcripts and the accumulation of gibberellin metabolites. This integrated analysis allowed us to uncover additional information for a more comprehensive understanding of biological events relevant to fungal secondary metabolic regulation in response to nitrogen availability.<\/jats:p>","DOI":"10.3390\/ijms241310832","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T00:43:30Z","timestamp":1688085810000},"page":"10832","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Integration of Transcriptomic and Metabolomic Profiles Provides Insights into the Influence of Nitrogen on Secondary Metabolism in Fusarium sacchari"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2764-5373","authenticated-orcid":false,"given":"Yixue","family":"Bao","sequence":"first","affiliation":[{"name":"State Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology & Academy of Sugarcane and Sugar Industry, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyue","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology & Academy of Sugarcane and Sugar Industry, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yao","sequence":"additional","affiliation":[{"name":"State Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology & Academy of Sugarcane and Sugar Industry, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sehrish","family":"Akbar","sequence":"additional","affiliation":[{"name":"State Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology & Academy of Sugarcane and Sugar Industry, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfeng","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology & Academy of Sugarcane and Sugar Industry, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles A.","family":"Powell","sequence":"additional","affiliation":[{"name":"IFAS Indian River Research and Education Center, University of Florida, Fort Pierce, FL 34945, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6623-1256","authenticated-orcid":false,"given":"Jianlong","family":"Xu","sequence":"additional","affiliation":[{"name":"Hainan Yazhou Bay Seed Laboratory, National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572025, China"},{"name":"Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3138-3422","authenticated-orcid":false,"given":"Muqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology & Academy of Sugarcane and Sugar Industry, Guangxi University, Nanning 530004, China"},{"name":"IFAS Indian River Research and Education Center, University of Florida, Fort Pierce, FL 34945, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1111\/j.1469-8137.2012.04300.x","article-title":"Nitrogen isotopes link mycorrhizal fungi and plants to nitrogen dynamics","volume":"196","author":"Hobbie","year":"2012","journal-title":"New. 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