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During the past decades, BPF contamination in the aquatic environment has dramatically increased due to its release from manmade products. Concerns have driven much attention to whether it may adversely impact aquatic lives or human beings. The present study performed an acute toxic exposure experiment and a 15 d developmental exposure of BPF at environmental concentrations (20, 200, and 2000 ng\/L) using Chinese medaka (Oryzias sinensis). In the acute toxic exposure, the LC50 of BPF to Chinese medaka is 87.90 mg\/L at 96 h. Developmental exposure induced a significant increase in the frequency of larvae with abnormalities in the 2000 ng\/L BPF group compared to the control group. Transcriptomic analysis of the whole larvae revealed 565 up-regulated and 493 down-regulated genes in the 2000 ng\/L BPF exposure group. Analysis of gene ontology and KEGG pathways enrichments indicated endocrine disorders to be associated with BPF-induced developmental toxicity. The present results suggest that BPF is developmentally toxic at 2000 ng\/L concentration in Chinese medaka and causes endocrine-related aberrations in the transcriptional network of genes.<\/jats:p>","DOI":"10.3390\/ijms241310898","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T16:06:37Z","timestamp":1688141197000},"page":"10898","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Transcriptome Analysis of the Developmental Effects of Bisphenol F Exposure in Chinese Medaka (Oryzias sinensis)"],"prefix":"10.3390","volume":"24","author":[{"given":"Zhiying","family":"Liang","sequence":"first","affiliation":[{"name":"Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafen","family":"Guo","sequence":"additional","affiliation":[{"name":"Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duan","family":"Pi","sequence":"additional","affiliation":[{"name":"Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingying","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongsi","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2583-1340","authenticated-orcid":false,"given":"Xiaohong","family":"Song","sequence":"additional","affiliation":[{"name":"The Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control, Guilin University of Technology, Guilin 541000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5608-7254","authenticated-orcid":false,"given":"Ramji Kumar","family":"Bhandari","sequence":"additional","affiliation":[{"name":"Department of Biology, University of North Carolina Greensboro, Greensboro, NC 27412, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4159-5402","authenticated-orcid":false,"given":"Xuegeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Modern Aquaculture Science and Engineering, Guangdong-Macao Joint Laboratory for Aquaculture Breeding Development and Innovation, College of Life Sciences, South China Normal University, Guangzhou 510631, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"138560","DOI":"10.1016\/j.chemosphere.2023.138560","article-title":"Bisphenols in water: Occurrence, effects, and mitigation strategies","volume":"328","author":"Loganathan","year":"2023","journal-title":"Chemosphere"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1093\/biolre\/ioaa169","article-title":"Bisphenol A and 17\u03b1-ethinylestradiol-induced transgenerational gene expression differences in the brain\u2013pituitary\u2013testis axis of medaka, Oryzias latipes","volume":"103","author":"Thayil","year":"2020","journal-title":"Biol. Reprod."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"105553","DOI":"10.1016\/j.aquatox.2020.105553","article-title":"Transcriptome analysis of testis reveals the effects of developmental exposure to bisphenol a or 17\u03b1-ethinylestradiol in medaka (Oryzias latipes)","volume":"225","author":"Bhandari","year":"2020","journal-title":"Aquat. Toxicol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"7014","DOI":"10.1073\/pnas.0502544102","article-title":"Estrogenic chemicals in plastic and oral contraceptives disrupt development of the fetal mouse prostate and urethra","volume":"102","author":"Timms","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.mce.2012.01.001","article-title":"The estrogenic endocrine disrupting chemical bisphenol A (BPA) and obesity","volume":"354","author":"Nagel","year":"2012","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Vrooman, L.A., Oatley, J.M., Griswold, J.E., Hassold, T.J., and Hunt, P.A. (2015). Estrogenic exposure alters the spermatogonial stem cells in the developing testis, permanently reducing crossover levels in the adult. PLoS Genet., 11.","DOI":"10.1371\/journal.pgen.1004949"},{"key":"ref_7","first-page":"10","article-title":"A dispersive liquid-liquid microextraction based on solidification of floating organic droplet followed by injector port silylation coupled with gas chromatography\u2013tandem mass spectrometry for the determination of nine bisphenols in bottled carbonated beverages","volume":"1528","author":"Mandrah","year":"2017","journal-title":"J. Chromatogr. A Incl. Electrophor. Other Sep. Methods"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1007\/s00244-018-0527-9","article-title":"Occurrence and Ecological Risk Assessment of Eight Endocrine-Disrupting Chemicals in Urban River Water and Sediments of South China","volume":"75","author":"Huang","year":"2018","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.watres.2016.07.059","article-title":"Occurrence and partitioning of bisphenol analogues in water and sediment from Liaohe River Basin and Taihu Lake, China","volume":"103","author":"Jin","year":"2016","journal-title":"Water Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1016\/j.scitotenv.2017.05.069","article-title":"Occurrence, distribution and sources of bisphenol analogues in a shallow Chinese freshwater lake (Taihu Lake): Implications for ecological and human health risk","volume":"599\u2013600","author":"Liu","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.ecoenv.2015.09.029","article-title":"Bisphenol A and other bisphenol analogues including BPS and BPF in surface water samples from Japan, China, Korea and India","volume":"122","author":"Yamazaki","year":"2015","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1016\/S0043-1354(01)00367-0","article-title":"Occurrence of phthalates and bisphenol A and F in the environment","volume":"36","author":"Fromme","year":"2002","journal-title":"Water Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.envpol.2017.03.018","article-title":"Fate and mass balance of bisphenol analogues in wastewater treatment plants in Xiamen City, China","volume":"225","author":"Sun","year":"2017","journal-title":"Environ. Pollut."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.chroma.2018.04.004","article-title":"Determination of nine bisphenols in sewage and sludge using dummy molecularly imprinted solid-phase extraction coupled with liquid chromatography tandem mass spectrometry","volume":"1552","author":"Sun","year":"2018","journal-title":"J. Chromatogr. A"},{"key":"ref_15","first-page":"744","article-title":"The occurrence and source identification of bisphenol compounds in wastewaters","volume":"616\u2013617","author":"Mislej","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_16","first-page":"2446","article-title":"The occurrence of contaminants of emerging concern in Slovenian and Croatian wastewaters and receiving Sava river","volume":"650","author":"Esen","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.scitotenv.2018.11.053","article-title":"Occurrence and exposure assessment of bisphenol analogues in source water and drinking water in China","volume":"655","author":"Zhang","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s12403-022-00483-3","article-title":"Occurrence and exposure assessment of bisphenol analogues through different types of drinking water in Korea","volume":"15","author":"Lim","year":"2023","journal-title":"Expo. Health"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1007\/s00244-014-0016-8","article-title":"A survey of alkylphenols, bisphenols, and triclosan in personal care products from China and the United States","volume":"67","author":"Liao","year":"2014","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.toxlet.2003.07.003","article-title":"Comparative study of the uterotrophic potency of 14 chemicals in a uterotrophic assay and their receptor-binding affinity","volume":"146","author":"Yamasaki","year":"2004","journal-title":"Toxicol. Lett."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"12796","DOI":"10.1021\/acs.est.7b03283","article-title":"Acute Toxicity, Teratogenic, and Estrogenic Effects of Bisphenol A and Its Alternative Replacements Bisphenol S, Bisphenol F, and Bisphenol AF in Zebrafish Embryo-Larvae","volume":"51","author":"Moreman","year":"2017","journal-title":"Environ. Sci. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"109870","DOI":"10.1016\/j.ecoenv.2019.109870","article-title":"Bisphenol F exposure impairs neurodevelopment in zebrafish larvae (Danio rerio)","volume":"188","author":"Gu","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"14638","DOI":"10.1021\/acs.est.9b04097","article-title":"Bisphenol F-Induced Neurotoxicity toward Zebrafish Embryos","volume":"53","author":"Yuan","year":"2019","journal-title":"Environ. Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.tox.2008.09.024","article-title":"Genotoxic and endocrine activities of bis(hydroxyphenyl)methane (bisphenol F) and its derivatives in the HepG2 cell line","volume":"255","author":"Cabaton","year":"2009","journal-title":"Toxicology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1016\/j.envpol.2017.11.027","article-title":"Bisphenol A alternatives bisphenol S and bisphenol F interfere with thyroid hormone signaling pathway in vitro and in vivo","volume":"237","author":"Zhang","year":"2018","journal-title":"Environ. Pollut."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.chemosphere.2015.12.080","article-title":"Waterborne exposure to bisphenol F causes thyroid endocrine disruption in zebrafish larvae","volume":"147","author":"Huang","year":"2016","journal-title":"Chemosphere"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"106190","DOI":"10.1016\/j.aquatox.2022.106190","article-title":"Long-term exposure of zebrafish to bisphenol F: Adverse effects on parental reproduction and offspring neurodevelopment","volume":"248","author":"Gu","year":"2022","journal-title":"Aquat. Toxicol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"157974","DOI":"10.1016\/j.scitotenv.2022.157974","article-title":"Bisphenol F induces liver-gut alteration in zebrafish","volume":"851","author":"Wang","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.scitotenv.2019.02.154","article-title":"In vivo actions of Bisphenol F on the reproductive neuroendocrine system after long-term exposure in zebrafish","volume":"665","author":"Qiu","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"105286","DOI":"10.1016\/j.yrtph.2022.105286","article-title":"Reproductive and developmental toxicity screening of bisphenol F by oral gavage in rats","volume":"136","author":"Lee","year":"2022","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"dvaa008","DOI":"10.1093\/eep\/dvaa008","article-title":"DNA methylation reprogramming in medaka fish, a promising animal model for environmental epigenetics research","volume":"6","author":"Wang","year":"2020","journal-title":"Environ. Epigenet."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1038\/nature13544","article-title":"The DNA methylation landscape of human early embryos","volume":"511","author":"Guo","year":"2014","journal-title":"Nature"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1080\/15592294.2019.1605816","article-title":"DNA methylation dynamics during epigenetic reprogramming of medaka embryo","volume":"14","author":"Wang","year":"2019","journal-title":"Epigenetics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1080\/15592294.2019.1695341","article-title":"The dynamics of DNA methylation during epigenetic reprogramming of primordial germ cells in medaka (Oryzias latipes)","volume":"15","author":"Wang","year":"2020","journal-title":"Epigenetics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1038\/nature751","article-title":"DMY is a Y-specific DM-domain gene required for male development in the medaka fish","volume":"417","author":"Matsuda","year":"2002","journal-title":"Nature"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1038\/nature05846","article-title":"The medaka draft genome and insights into vertebrate genome evolution","volume":"447","author":"Kasahara","year":"2007","journal-title":"Nature"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"127813","DOI":"10.1016\/j.chemosphere.2020.127813","article-title":"Oryzias sinensis, a new model organism in the application of eco-toxicity and water quality criteria (WQC)","volume":"261","author":"Cui","year":"2020","journal-title":"Chemosphere"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"120724","DOI":"10.1016\/j.envpol.2022.120724","article-title":"Genome and transcriptome of Chinese medaka (Oryzias sinensis) and its uses as a model fish for evaluating estrogenicity of surface water","volume":"317","author":"Wang","year":"2023","journal-title":"Environ. Pollut."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1021\/acs.est.2c05753","article-title":"Reproductive Toxicity and Teratogenicity of Fluorene-9-bisphenol on Chinese Medaka (Oryzias sinensis): A Study from Laboratory to Field","volume":"57","author":"Fan","year":"2023","journal-title":"Environ. Sci. Technol."},{"key":"ref_40","first-page":"251","article-title":"Comparison on Acute Toxicity of Bisphenol A with Its Substitutes to Pelophylax nigromaculatus","volume":"10","author":"Li","year":"2015","journal-title":"Asian J. Ecotoxicol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"106154","DOI":"10.1016\/j.aquatox.2022.106154","article-title":"Assessing the toxicity of bisphenol A and its six alternatives on zebrafish embryo\/larvae","volume":"246","author":"Gao","year":"2022","journal-title":"Aquat. Toxicol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.chemosphere.2017.06.010","article-title":"Bisphenol analogues in surface water and sediment from the shallow Chinese freshwater lakes: Occurrence, distribution, source apportionment, and ecological and human health risk","volume":"184","author":"Yan","year":"2017","journal-title":"Chemosphere"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2269","DOI":"10.1002\/jssc.201701087","article-title":"Simultaneous identification and quantification of bisphenol A and 12 bisphenol analogues in environmental samples using precolumn derivatization and ultra high performance liquid chromatography with tandem mass spectrometry","volume":"41","author":"Wang","year":"2018","journal-title":"J. Sep. Sci."},{"key":"ref_44","first-page":"220","article-title":"The Role of Plant Peroxisomes in ROS Signalling Network","volume":"33","author":"Cui","year":"2017","journal-title":"Zhongguo Shengwu Huaxue Yu Fenzi Shengwu Xuebao"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.chemosphere.2018.06.089","article-title":"Bisphenol A and its analogs bisphenol B, bisphenol F, and bisphenol S: Comparative in vitro and in vivo studies on the sperms and testicular tissues of rats","volume":"209","author":"Ullah","year":"2018","journal-title":"Chemosphere"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chemosphere.2017.11.125","article-title":"Immunotoxicity of bisphenol S and F are similar to that of bisphenol A during zebrafish early development","volume":"194","author":"Qiu","year":"2018","journal-title":"Chemosphere"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.micres.2017.08.006","article-title":"Apoptosis induced by Staphylococcus aureus toxins","volume":"205","author":"Zhang","year":"2017","journal-title":"Microbiol. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.ecoenv.2019.01.126","article-title":"Metabolism disruption analysis of zebrafish larvae in response to BPA and BPA analogs based on RNA-Seq technique","volume":"174","author":"Qiu","year":"2019","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"379","DOI":"10.2108\/zsj.22.379","article-title":"Molecular regulation of gonadotropin secretion by gonadotropin-releasing hormone in salmonid fishes","volume":"22","author":"Ando","year":"2005","journal-title":"Zool. Sci."},{"key":"ref_50","unstructured":"OECD (1998). Test No. 212: Fish, Short-Term Toxicity Test on Embryo and Sac-Fry Stages, OECD Publishing."},{"key":"ref_51","unstructured":"OECD (2013). Test No. 210: Fish, Early-Life Stage Toxicity Test, OECD Publishing."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1038\/nprot.2016.095","article-title":"Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown","volume":"11","author":"Pertea","year":"2016","journal-title":"Nat. Protoc. Erecipes Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Dewey, C.N., and Bo, L. (2011). RSEM: Accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-323"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene Ontology: Tool for the unification of biology","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/13\/10898\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:03:29Z","timestamp":1760126609000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/13\/10898"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,30]]},"references-count":58,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["ijms241310898"],"URL":"https:\/\/doi.org\/10.3390\/ijms241310898","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,30]]}}}